Review
Endocrinology & Metabolism
Emma Bokobza, Charlotte Hinault, Victor Tiroille, Stephan Clavel, Frederic Bost, Nicolas Chevalier
Summary: Obesity is a significant public health concern associated with increased risk of various cancers, including gastrointestinal tract cancers and hormone-dependent cancers. Adipose tissue plays a crucial role in this process, as it is considered a major internal source of endocrine disrupting chemicals that can impact hormonal balance and cell proliferation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Analytical
Sang Jun Park, Myoung Ki Cho, Dong Joon Lee, Deuk Hwa Song, Chang Su Lim, Choong-Kyun Noh, Dong Min Lee, Kyeong Sook Choi, Sung Jae Shin, Hwan Myung Kim
Summary: A two-photon probe SCX was developed to reflect the level of COX-2 in colorectal cancer patients and distinguish between cancerous and normal tissue. In vitro and cell imaging studies showed that SCX could effectively differentiate the expression levels of COX-2 in cancerous and normal cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Mikhail Raevskiy, Maxim Sorokin, Galina Zakharova, Victor Tkachev, Nicolas Borisov, Denis Kuzmin, Kristina Kremenchutckaya, Alexander Gudkov, Dmitry Kamashev, Anton Buzdin
Summary: Analyzing data at the pathway activation level can yield more consistent results when comparing RNA and protein expression profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Xing-Xing Fan, Qiang Wu
Summary: Lung cancer is highly heterogeneous, and single-cell analysis can help us understand its molecular characteristics and cell types better. Recent findings may provide new directions for the treatment strategies of lung cancer.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Zhuo Ma, Xiaofei Zhang, Wen Zhong, Hongyan Yi, Xiaowei Chen, Yinsuo Zhao, Yanlin Ma, Eli Song, Tao Xu
Summary: In this study, the authors used single-cell RNA sequencing and single-cell assay for transposase accessible chromatin sequencing to explore the molecular heterogeneity, developmental trajectory, and regulatory networks of early human pancreas development. They identified differences in gene expression patterns between dorsal and ventral pancreatic multipotent cells in humans, and identified pancreato-biliary progenitors that generate ventral multipotent cells. Notch and MAPK signals from mesenchymal cells were found to regulate the differentiation of multipotent cells into trunk and duct cells. They also discovered different subclusters of endocrine progenitors with varying differentiation potentials. While the developmental trajectories were largely conserved between humans and mice, distinct gene expression patterns were also identified.
NATURE COMMUNICATIONS
(2023)
Article
Neurosciences
Valerie Dietz, Katelyn Knox, Sherilynne Moore, Nolan Roberts, Karla Kassandra Corona, Jennifer N. Dulin
Summary: Spinal cord injury often leads to immediate and sustained neurological dysfunction, including neuropathic pain. This study found that the severity of the injury negatively correlated with the manifestation of mechanical hypersensitivity and suggests that the protection of dorsal horn neurons may be required for the development of neuropathic pain.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Peripheral Vascular Disease
Alessandra Pasut, Lisa M. Becker, Anne Cuypers, Peter Carmeliet
Summary: The plasticity of vascular endothelial cells drives physiological repair and pathological angiogenesis, posing challenges for targeted therapy due to their heterogeneity and unidentified phenotypes. Single-cell studies have revealed the diversity of EC types and states, offering insights into new subtypes and functions in health and disease. Metabolic gene signatures in different EC phenotypes may hold therapeutic potential for promoting tissue repair or inhibiting tumor angiogenesis in a tissue-specific manner.
Article
Biochemical Research Methods
Ruiyao Cai, Zeynep Ilgin Kolabas, Chenchen Pan, Hongcheng Mai, Shan Zhao, Doris Kaltenecker, Fabian F. Voigt, Muge Molbay, Tzu-lun Ohn, Cecile Vincke, Mihail I. Todorov, Fritjof Helmchen, Jo A. Van Ginderachter, Ali Ertuerk
Summary: A nanobody-based immunolabeling and clearing method is reported, which renders a whole mouse transparent in 3 weeks and enhances the signal of fluorescent proteins. This method allows the analysis of fluorescent signal in intact rodents at cellular resolution and enables the study of whole-body phenomena and connectivity.
Article
Biochemistry & Molecular Biology
Lorena Mazuecos, Cristina Pintado, Blanca Rubio, Eduardo Guisantes-Batan, Antonio Andres, Nilda Gallardo
Summary: The study demonstrates that PPAR beta/delta is involved in the up-regulation of FGF21 expression induced by leptin in visceral adipose tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemistry & Molecular Biology
Patrick R. H. Steinmetz
Summary: A new study discovers genetic similarities between the segmental pouches of sea anemones and the segmented structures of vertebrates and arthropods.
Article
Biochemical Research Methods
Jackson B. Coole, David Brenes, Julio Cesar Possati-Resende, Marcio Antoniazzi, Bruno De Oliveira Fonseca, Yajur Maker, Alex Kortum, Imran S. Vohra, Richard A. Schwarz, Jennifer Carns, Karen Cristina Borba Souza, Iara Viana Vidigal Santana, Regis Kreitchmann, Mila P. Salcedo, Nirmala Ramanujam, Kathleen M. Schmeler, Rebecca Richards-Kortum
Summary: Cervical cancer is a leading cause of cancer death among women in low- and middle-income countries. The lack of resources, infrastructure, and personnel hinders cervical cancer prevention programs globally. This study presents a multimodal mobile colposcope that can diagnose precancerous cervical lesions at the point-of-care, overcoming the limitations of high-resolution imaging.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biodiversity Conservation
Ping Xie, Weijun He, Min An, Meng Fan, Xiaohua Dong
Summary: This article analyzes the relationship between ecological environment quality (EEQ) and economic development (ED) at the industrial park scale by constructing a new remote sensing ecological index model and an ED index system. The results show that the ecological environment of the industrial park remains relatively stable while economic development continues to grow, and the relationship between the two maintains a certain level of coordination.
ECOLOGICAL INDICATORS
(2023)
Review
Biochemistry & Molecular Biology
Chien-Hsiu Li, Tai- Hsu, Yu-Chan Chang, Ming-Hsien Chan, Pei-Jung Lu, Michael Hsiao
Summary: Epithelial and mesenchymal transition mechanisms occur continuously during the cell cycle and human development. EMT in embryo development involves three steps: cell formation, subdivision, and valve formation. Malignant tumor cells acquire immortality characteristics, invade blood vessels through EMT, and undergo MET to form secondary tumors.
Article
Biochemistry & Molecular Biology
Yue Yu, Xinjie Li, Rui Jiao, Yang Lu, Xuan Jiang, Xin Li
Summary: The transition of H3K27me3 to H3K4me1 at bivalent promoters plays a crucial role in lineage differentiation by regulating tissue-specific gene expression. LSD1 interacts with PRC2 and contributes to this transition.
CELL AND BIOSCIENCE
(2023)
Review
Polymer Science
Yingxuan Zhu, Mengqi Zhang, Qingqing Sun, Xiaofeng Wang, Xiaomeng Li, Qian Li
Summary: Mechanics plays a crucial role in cell function and tissue regeneration, and the mechanical detection of cell mechanics and cellular-scale microenvironments is essential for understanding cell activities affected by physical factors. This review focuses on advanced techniques and applications of cell-scale mechanical detection, including atomic force microscopy, optical tweezer, magnetic tweezer, and traction force microscopy. It also discusses the application of mechanical testing techniques to cell mechanics and tissue engineering scaffolds. The challenges and prospects in this field are highlighted, providing valuable insights into micromechanics in tissue engineering.
