Article
Plant Sciences
Palaniyandi Karuppaiya, Jun Wu
Summary: This study reports the successful induction of callus from Dysosma versipellis explants and the production of tumor inhibitory compounds. It was found that tender leaves exhibited the highest callus induction efficiency, and roots and rhizomes were the primary source of secondary compounds accumulation.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Selvaraj Vimalraj
Summary: Angiogenesis is the formation of new blood vessels from existing ones. Abnormal angiogenesis, seen in tumor microenvironments, is characterized by distorted and leaky blood vessels with various shapes and high perfusion efficiency. Tumor angiogenesis plays a crucial role in cancer growth, invasion, and metastasis, and is tightly regulated by signaling networks. Targeting multiple signaling pathways involved in angiogenesis is important for effective antiangiogenic therapy. New therapeutic approaches, such as blocking specific proteins or genes, are being explored to dissect alternative angiogenesis mechanisms in tumor microenvironments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Integrative & Complementary Medicine
Yuan -Hui Guo, Peng Lu, Jia-Xing He, Bao-Nan Ma, Ebuka-Olisaemeka Nwafor, Wen-Xin Wang, Chuan-Xin Liu
Summary: This article summarizes the chemical constituents, pharmacological activities, and toxicological effects of Dysosma versipellis. It also predicts and analyzes the plant's genetic characteristics, specific chemical compositions, traditional medicinal properties, efficacy, chemical composition measurability, blood components, and pharmacokinetics. The findings provide a reference for future research on Dysosma versipellis.
TRADITIONAL MEDICINE RESEARCH
(2023)
Article
Cell Biology
Ryan O. Snodgrass, Karan Govindpani, Karen Plant, Elisabeth C. Kugler, Changmin Doh, Thomas Dawson, Luis E. McCormack, Helen M. Arthur, Timothy J. A. Chico
Summary: Hereditary haemorrhagic telangiectasia (HHT) is caused by mutations in the BMP co-receptor endoglin, leading to arteriovenous malformations (AVMs) and various complications. Zebrafish models were used to study the vascular phenotypes associated with endoglin mutations and the effect of inhibiting Vegf signalling pathways. Inhibiting mTOR or MEK pathways prevented abnormal vasculature phenotypes, suggesting a potential therapeutic strategy in HHT. These findings highlight the importance of Vegf signalling modulation in mitigating HHT-like phenotypes.
DISEASE MODELS & MECHANISMS
(2023)
Article
Cell Biology
Ekta Manocha, Antonella Bugatti, Mirella Belleri, Alberto Zani, Stefania Marsico, Francesca Caccuri, Marco Presta, Arnaldo Caruso
Summary: ARV p17 is a non-structural protein that activates autophagy, inhibits angiogenesis in human endothelial cells, and renders cells insensitive to proangiogenic inducers. It upregulates the release of the tumor suppressor molecule DPP4, highlighting its potential antitumor activities and suggesting new therapeutic approaches for treating tumors and cancer metastasis.
Article
Oncology
Jing Liu, Fang Zhang, Jie He, Shuyun Wang, Leirong Wang, Juan Li, Wenjing Shi, Yali Han, Aiqin Gao, Yuping Sun
Summary: Current anti-angiogenic therapies have changed the paradigm of treating colorectal cancer (CRC) patients with advanced diseases. However, the clinical response rate is still low at less than 10% due largely to complex angiogenic factors released by tumor cells. Exploring novel mechanisms of tumor angiogenesis and identifying alternative targets for combination therapies are therefore essential to effective inhibition of tumor vascularization and CRC development.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Review
Medicine, General & Internal
Kaja Michalczyk, Natalia Niklas, Malgorzata Rychlicka, Aneta Cymbaluk-Ploska
Summary: Endometrial cancer is a common gynecological neoplasm in developed countries with high early diagnosis rates and good prognosis, but a high recurrence rate in later stages. Factors secreted by adipose tissue play a significant role in regulating glucose and lipid metabolism and influence the diagnosis and prognosis of endometrial cancer.
Review
Biochemistry & Molecular Biology
Jhansi Nathan, Arunkumar Ramachandran
Summary: Marine biomolecules with antiangiogenic properties show potential for cancer treatment, with small molecular drug screening using the zebrafish model as an advantage in recent cancer research.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Review
Biology
Jean Eberlein, Lukas Herdt, Julian Malchow, Annegret Rittershaus, Stefan Baumeister, Christian S. M. Helker
Summary: Establishing a functional cardiovascular system is crucial for vertebrate development, with defects leading to cardiovascular diseases. Zebrafish has been instrumental in identifying novel mechanisms of vascular growth, particularly in understanding the molecular pathways involved in blood vessel formation within different tissues and organs.
Article
Developmental Biology
Elvin Leonard, Sana Safatul Hasan, Arndt F. Siekmann
Summary: Blood vessels in the zebrafish caudal fin form through a stepwise process involving different morphogenetic processes, including artery generation from vein-derived endothelial cells, production of veins by veins, formation of distinct vascular plexuses, and angiogenesis. These processes are regulated by a combination of signaling pathways and local cues. The study provides insights into the diverse mechanisms underlying the assembly of mature vasculature.
Article
Multidisciplinary Sciences
Manusmriti Agarwal, Ankush Sharma, R. Andrea Kagoo, Anamika Bhargava
Summary: As the global demand for new synthetic compounds continues to grow, the adverse health effects of these compounds have become a critical public health concern. Zebrafish, with their genomic similarity to humans, optical clarity, well-defined developmental stages, short generation time, and cost-effective maintenance, have emerged as a prominent model organism for toxicology studies. The use of zebrafish allows for faster in vivo toxicology evaluation compared to mammalian experimental systems.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Jing Chen, Yingchao Wang, Shufang Wang, Xiaoping Zhao, Lu Zhao, Yi Wang
Summary: This study demonstrated the significant pro-angiogenic effects of Guanxinning Tablet (GXNT) and its major components, Sal B and FA, in enhancing vascular growth by regulating the expression of VEGF receptors and ligands. Further research is needed to explore the molecular interactions between these compounds and the key regulators in neovascularization.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Zoology
MA Liman, QI Yongxiao, Wang Wenji, Zhong Qianyi
Summary: Ginsenoside Rh2 (G-Rh2), a natural product derived from red ginseng, has been reported to have therapeutic effects on some tumors, and its antiangiogenic effects in zebrafish were investigated in this study. It was found that G-Rh2 exerted anti-angiogenic activity in zebrafish embryos by downregulating Vegf expression.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
Article
Cell Biology
Francesca Maradonna, Camilla M. Fontana, Fiorenza Sella, Christian Giommi, Nicola Facchinello, Chiara Rampazzo, Micol Caichiolo, Seyed Hossein Hoseinifar, Luisa Dalla Valle, Hien Van Doan, Oliana Carnevali
Summary: Colon cancer is a leading cause of death worldwide. This study evaluated the effects of anandamide (AEA) exposure in zebrafish embryos with transplanted HCT116 cells. The results showed that AEA could reduce tumor size, inhibit tumor angiogenesis, and prevent micrometastasis formation. Molecular analyses further demonstrated that AEA downregulated genes involved in cell proliferation, angiogenesis, and the immune system. The study suggests the efficacy of AEA in personalized cancer therapy and supports the use of zebrafish xenograft as a model for cancer studies.
CELL DEATH & DISEASE
(2022)
Article
Anatomy & Morphology
Yuka Haneda, Sachiko Miyagawa-Tomita, Yasunobu Uchijima, Akiyasu Iwase, Rieko Asai, Takahide Kohro, Youichiro Wada, Hiroki Kurihara
Summary: This study demonstrates the novel roles of ASCs, especially in heart and thyroid development. It sheds light on the cardiovascular development of amniotes from both embryological and evolutionary perspectives.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Fisheries
Bingxi Li, Yuan Li, Siwei Wu, Yanjian Yang, Shengli Fu, Xiaoxue Yin, Xiao Tu, Liang Fang, Zheng Guo, Jianmin Ye
Summary: CD154, a multifunctional molecule highly expressed in activated T cells and important for T cell-dependent humoral immune response, was identified in Nile tilapia. The mRNA of OnCD154 is highly expressed in immune organs, and an anti-OnCD154 polyclonal antibody was successfully prepared to react with natural protein. The study also demonstrated that OnCD154 might be involved in T cell-dependent immune response and provided new insights into the humoral immune response of teleost fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Nutrition & Dietetics
Taida Huang, Mo Yang, Yunxin Zeng, Xiaomin Huang, Nan Wang, Yun Chen, Peng Li, Jinqiu Yuan, Chun Chen, Brian G. Oliver, Chenju Yi
Summary: The study found that maternal smoking during pregnancy increased body weight and fat mass in HFD-fed offspring, while offspring exposed to both maternal smoking and HFD showed the highest energy intake and glucose metabolic disorder in adulthood. In addition, postnatal HFD consumption increased body weight and worsened metabolic disorders caused by maternal smoking and maternal smoking combined with HFD.
FRONTIERS IN NUTRITION
(2021)
Review
Neurosciences
Xiaomin Huang, Yixun Su, Nan Wang, Hui Li, Zhigang Li, Guowei Yin, Hui Chen, Jianqin Niu, Chenju Yi
Summary: Astrocytes play a crucial role in maintaining the normal functions of the Central Nervous System (CNS). During neurodegenerative diseases, alterations in astrocytic connexin expression and function can affect disease progression by impacting neuronal function and survival. Targeting connexins may be a plausible therapeutic strategy for managing these neurodegenerative diseases.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Physiology
Yunxin Zeng, Taida Huang, Nan Wang, Yi Xu, Chunhui Sun, Min Huang, Chun Chen, Brian G. Oliver, Chenju Yi, Hui Chen
Summary: Maternal cigarette smoke exposure results in lighter body weight and glucose intolerance in offspring, while postnatal L-leucine supplementation can reduce fat accumulation and ameliorate glucose metabolic disorder caused by maternal smoke exposure.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Yunxing Liu, Fang Liang, Zijiong Dong, Song Li, Jianmin Ye, Wei Qin
Summary: ScCas9, derived from Streptococcus canis, shows editing activity in zebrafish and can induce mutations in the genome. Utilizing a ribonucleoprotein complex and synthetic modified crRNA:tracrRNA duplex, the editing ability of ScCas9 in zebrafish has been optimized for targeting relaxed NNG PAMs.
Article
Fisheries
Bingxi Li, Yanjian Yang, Yuan Li, Liting Wu, Siwei Wu, Zijiong Dong, Meng Chen, Fang Liang, Zheng Guo, Bei Wang, Jianmin Ye
Summary: The study identified and characterized the Nile tilapia CD40-like protein, OnCD40, and revealed its important role in the TD immune response, particularly in the expression patterns during B cell activation and differentiation. Furthermore, the study found that the expression level of OnCD40 was higher in IgMhi B cells compared to IgMlo plasma-like B cells.
Article
Neurosciences
Taida Huang, Xiaomin Huang, Hui Li, Junhua Qi, Nan Wang, Yi Xu, Yunxin Zeng, Xuewen Xiao, Ruide Liu, Yik Lung Chan, Brian G. Oliver, Chenju Yi, Dan Li, Hui Chen
Summary: Maternal smoke exposure worsens neurological defects in females with hypoxic-ischemic brain injury, potentially through oxidative stress and inflammatory responses.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Fisheries
Zijiong Dong, Bingxi Li, Liting Wu, Yang Lei, Jianlin Chen, Liangliang Mu, Hairong Wu, Meng Chen, Bei Wang, Jianmin Ye, Fang Liang
Summary: The study identified and characterized two paralogous class B scavenger receptors (scarb) in Nile tilapia, which were markedly up-regulated in liver and head kidney after pathogen infection, suggesting their important roles in innate immune responses. Multiple sequence alignment and phylogenetic tree analyses revealed high sequence identity/similarity with counterparts from other teleost species, indicating close evolutionary relationship within vertebrates.
AQUACULTURE REPORTS
(2021)
Article
Multidisciplinary Sciences
Fang Liang, Yu Zhang, Lin Li, Yexin Yang, Ji-Feng Fei, Yanmei Liu, Wei Qin
Summary: The development of PAM-less base editors enables the assessment of functional impact and pathogenicity of nucleotide mutations in animals. This study successfully optimized SpG and SpRY systems in zebrafish and generated base editors mediated by SpRY. These tools can efficiently and precisely edit the zebrafish genome, providing strong support for revealing gene function, physiological mechanisms, and disease pathogenesis.
NATURE COMMUNICATIONS
(2022)
Article
Fisheries
Zijiong Dong, Jiadong Li, Ruhuang Deng, Zhenzhen Zhang, Jianlin Chen, Yang Lei, Liting Wu, Zheng Guo, Bei Wang, Bingxi Li, Fang Liang, Jianmin Ye
Summary: In this study, two forms of TLR5, OnTLR5M and OnTLR5S, were cloned and identified in Nile tilapia. They were found to be significantly up-regulated after pathogen stimulation and involved in the immune response. The results suggest that OnTLR5S, in cooperation with OnTLR5M, can activate NF-kappa B through signaling cascades to resist pathogen infection.
Article
Biochemistry & Molecular Biology
Fang Liang, Zijiong Dong, Jianmin Ye, Wei Hu, Ramji Kumar Bhandari, Kangsen Mai, Xuegeng Wang
Summary: This study demonstrates that CRISPR/dCas9-Dnmt7 and -Tet2 catalytic domain fusions can site-specifically edit genomic DNA methylation in zebrafish, and may serve as an efficient toolkit for DNA methylation editing in the zebrafish model.
Correction
Multidisciplinary Sciences
Fang Liang, Yu Zhang, Lin Li, Yexin Yang, Ji-Feng Fei, Yanmei Liu, Wei Qin
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Shaohui Zheng, Fang Liang, Yu Zhang, Ji-Feng Fei, Wei Qin, Yanmei Liu
Summary: CRISPR/Cas9 technology has enhanced the utility of zebrafish in modeling human genetic diseases and studying disease mechanisms. However, the limitations of protospacer adjacent motif (PAM) have hindered the creation of accurate animal models for human genetic disorders caused by single-nucleotide variants. In this study, an optimized adenine base editor and synthetically modified gRNA were utilized to enable efficient base conversion in zebrafish without PAM restriction, leading to the successful construction of a zebrafish disease model for studying disease mechanisms and potential treatments.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biology
Fang Liang, Xiaochan Lu, Biyu Wu, Yexin Yang, Wei Qin
Summary: Ribosomopathies are a series of disorders caused by ribosomal dysfunction, usually resulting in tissue-specific defects such as anemia. Defects in nucleolar proteins have been observed in human ribosomopathies, and it is unknown whether other ribosome proteins are involved in regulating erythropoiesis. In this study, a nop56 deficiency in zebrafish caused severe morphological abnormalities and anemia. The erythroid lineage and the maturation of erythroid cells were impaired in the nop56 mutants. Abnormal activation of the p53 and JAK2-STAT3 signaling pathways was observed, and partial rescue of the malformation and anemia was achieved through the inhibition of these pathways.
