Article
Endocrinology & Metabolism
Leolo Gonay, Catherine Spourquet, Matthieu Baudoin, Ludovic Lepers, Pascale Lemoine, Alexander G. Fletcher, Emmanuel Hanert, Christophe E. Pierreux
Summary: This study presents a 2D vertex model of thyroid growth, angiogenesis, and folliculogenesis that accurately simulates the differential growth of epithelial cells, migration of endothelial cells, and formation of follicular lumens during thyroid embryonic development. The model highlights the importance of cellular adhesion, movement, and polarization in organogenesis, providing valuable insights into the roles of these processes in thyroid function. By incorporating theoretical and experimental approaches, in silico models like the one described in this study have the potential to advance the fields of developmental biology and regenerative medicine.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Jifeng Liu, Hongyan Long, Dagmar Zeuschner, Andreas F. B. Rader, William J. Polacheck, Horst Kessler, Lydia Sorokin, Britta Trappmann
Summary: Current tissue engineering strategies lack materials that promote angiogenesis. Here the authors develop a microfluidic in vitro model in which chemokine-guided endothelial cell sprouting into a tunable hydrogel is followed by the formation of perfusable lumens to determine the material properties that regulate angiogenesis.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Daniel S. Levic, Michel Bagnat
Summary: A common phenomenon in animal development is the formation of fluid-filled cavities or lumina, which facilitate the transportation of gases and fluids across tissues and organs. Despite variations in size, scale, and complexity among different species, the processes of lumen formation share fundamental morphogenetic principles. This article explores the role of polarized targeting of membrane and secreted proteins, with a focus on ion transporters in vertebrate model systems, in regulating lumen formation. Mechanistic differences between invertebrates and vertebrates are discussed, as well as the involvement of Na+/K+ ATPase and junctional proteins in the polarization of immature epithelia to build lumina in developing organs.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Soojung Lee, Jonathan Chang, Sung-Min Kang, Eric Parigoris, Ji-Hoon Lee, Yun Suk Huh, Shuichi Takayama
Summary: This manuscript presents a new method for forming basal-in MCF10A organoids using commercial 384-well ultra-low attachment microplates. The development of associated live-cell imaging and automated analysis protocols allows for improved tracking of organoid formation and growth. The results and analysis protocol described in this study can serve as a practical resource for future research in this area.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Hiroshi Kurosaka, Jin Mushiake, Mithun Saha, Yanran Wu, Qi Wang, Masataka Kikuchi, Akihiro Nakaya, Sayuri Yamamoto, Toshihiro Inubushi, Satoshi Koga, Lisa L. Sandell, Paul A. Trainor, Takashi Yamashiro
Summary: By utilizing mouse models, the study uncovers the crucial role of the Gata3 gene in clinical pediatric diseases, while also revealing the critical synergy of Gata3 and retinoid signaling during embryonic development.
HUMAN MOLECULAR GENETICS
(2021)
Article
Cell Biology
Jingqiang Wang, Wenqian Song, Ruikai Yang, Chao Li, Ting Wu, Xiao Bing Dong, Bin Zhou, Xizhi Guo, Jianfeng Chen, Zhiyong Liu, Qing Cissy Yu, Wen Li, Junfen Fu, Yi Arial Zeng
Summary: This study reveals a crucial signaling axis in which fibroblasts relay Wnt signals from endothelial cells to organize epithelial patterning. It also demonstrates the importance of different niche components in coordinating epithelial organogenesis and tissue homeostasis.
Article
Biochemical Research Methods
Paul M. Van Liedekerke, Lila M. Gannoun, Axelle M. Loriot, Tim M. Johann, Frederic P. M. Lemaigre, Dirk M. Drasdo
Summary: The formation of biliary lumens involves coordination of various cellular mechanisms such as cell division, polarization, and fluid secretion. Successful lumen formation requires contributions from directed cell division, local osmotic effects from salt excretion, and controlled differentiation of cells, with apical constriction playing a moderate role. Understanding these processes is crucial for identifying intervention points to address defective bile ducts in development or disease.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Cell Biology
Kun Han, Feiyu Wang, Yulu Yue, Xihong Tan, Miao Tian, Yiliang Miao, Shuhong Zhao, Weijie Dong, Mei Yu
Summary: The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated alpha 2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine luminal epithelium.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Maria P. Kotini, Miesje M. van der Stoel, Jianmin Yin, Mitchell K. Han, Bettina Kirchmaier, Johan de Rooij, Markus Affolter, Stephan Huveneers, Heinz-Georg Belting
Summary: Blood vessel morphogenesis is driven by coordinated endothelial cell behaviors. Active remodeling of cell-cell junctions promotes cellular plasticity while preserving vascular integrity. Lumen expansion in angiogenic sprouts is accompanied by the formation of transient finger-shaped junctions. Blood pressure positively regulates the formation of junctional fingers, while flow inhibition prevents their formation. Mechanotransduction protein vinculin is recruited to junctional fingers, and its genetic deletion prevents finger formation. Endothelial cells employ force-dependent junctional remodeling to counteract external forces and maintain vascular integrity during sprouting angiogenesis.
Article
Multidisciplinary Sciences
Johanna Wagner, Markus Masek, Andrea Jacobs, Charlotte Soneson, Sujana Sivapatham, Nicolas Damond, Natalie de Souza, Mark D. Robinson, Bernd Bodenmiller
Summary: In this study, the EMT transition phenotypes in four non-cancerous human mammary epithelial cell lines were comprehensively profiled and compared to breast cancer cell lines. The results revealed a spectrum of EMT transition phenotypes in each cell line. These findings contribute to a better understanding of the complexity of EMT and provide valuable insights for the design of EMT time course experiments.
Article
Developmental Biology
Alejandro Torres-Sanchez, Max Kerr Winter, Guillaume Salbreux
Summary: This review explores the physical principles involved in lumen formation and coarsening, focusing on the roles of solute pumping, hydraulic flows, pressure differences, balance of forces, and electro-osmotic effects. The framework of linear irreversible thermodynamics is used to discuss the relevant force, time, and length scales. Order of magnitude estimates of physical parameters controlling lumen formation and coarsening are emphasized.
CELLS & DEVELOPMENT
(2021)
Review
Biochemistry & Molecular Biology
Swarajit Kumar Biswas, Saswati Banerjee, Ginger Wendolyn Baker, Chieh-Yin Kuo, Indrajit Chowdhury
Summary: The mammary gland, a compound and branched structure, plays a crucial role in mammalian reproduction by producing milk. Its development involves hormonal regulation and different stages of morphogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Rongze Ma, Difei Gong, Huanyang You, Chongshen Xu, Yunzhe Lu, Gabriele Bergers, Zena Werb, Ophir D. Klein, Claudia K. Petritsch, Pengfei Liu
Summary: The modulation of Integrin 01 signaling by Lgl1 is essential for directional migration, and epithelial branching in the mammary gland differs fundamentally from invertebrates.
Article
Multidisciplinary Sciences
Mariam Veschgini, Ryo Suzuki, Svenja Kling, Hendrik O. Petersen, Bruno Gideon Bergheim, Wasim Abuillan, Philipp Linke, Stefan Kaufmann, Manfred Burghammer, Ulrike Engel, Frank Stein, Suat Oezbek, Thomas W. Holstein, Motomu Tanaka
Summary: This study found that Wnt/beta-catenin signaling induces extracellular matrix (ECM) remodeling during Hydra axis formation. The arrangement of type I collagen in the ECM along Hydra's body axis was determined using high-resolution microscopy and X-ray scattering. Elasticity mapping of the ECM showed distinctive patterns along the body axis, which correlated with a gradient-like distribution of metalloproteases. Activation of the Wnt/beta-catenin pathway altered these patterns, resulting in low ECM elasticity. This study suggests that the spatiotemporal coordination of biochemical and biomechanical cues in ECM formation is a central evolutionary innovation for animal tissue morphogenesis.
Article
Microbiology
T. Essock-Burns, B. D. Bennett, D. Arencibia, S. Moriano-Gutierrez, M. Medeiros, M. J. McFall-Ngai, E. G. Ruby
Summary: This study demonstrates that quorum-sensing regulation by the Vibrio fischeri population induces a tissue phenotype that promotes the retention of this extracellular symbiont within the light organ of its host. Actin polymerization is identified as the primary mechanism underlying constriction, and host responses to the presence of symbionts change as a function of tissue maturation.
Article
Multidisciplinary Sciences
Elisa Manieri, Cintia Folgueira, Maria Elena Rodriguez, Luis Leiva-Vega, Laura Esteban-Lafuente, Chaobo Chen, Francisco Javier Cubero, Tamera Barrett, Julie Cavanagh-Kyros, Davide Seruggia, Alejandro Rosell, Fatima Sanchez-Cabo, Manuel Jose Gomez, Maria J. Monte, Jose J. G. Marin, Roger J. Davis, Alfonso Mora, Guadalupe Sabio
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Cell Biology
Myoung Sook Han, Rachel J. Perry, Joao-Paulo Camporez, Philipp E. Scherer, Gerald I. Shulman, Guangping Gao, Roger J. Davis
Summary: This study demonstrates that JNK signaling in adipocytes results in an increased circulating concentration of FGF21, regulating systemic metabolism. This mechanism of organ crosstalk involves a feed-forward regulatory loop mediated by JNK-regulated FGF21 autocrine signaling in adipocytes, promoting the expression of adiponectin and hepatic expression of FGF21. The novel signaling paradigm connects autocrine and endocrine signaling modes of the same hormone in different tissues.
GENES & DEVELOPMENT
(2021)
Article
Biology
Maria Crespo, Barbara Gonzalez-Teran, Ivana Nikolic, Alfonso Mora, Cintia Folgueira, Elena Rodriguez, Luis Leiva-Vega, Aranzazu Pintor-Chocano, Macarena Fernandez-Chacon, Irene Ruiz-Garrido, Beatriz Cicuendez, Antonia Tomas-Loba, Noelia A-Gonzalez, Ainoa Caballero-Molano, Daniel Beiroa, Lourdes Hernandez-Cosido, Jorge L. Torres, Norman J. Kennedy, Roger J. Davis, Rui Benedito, Miguel Marcos, Ruben Nogueiras, Andres Hidalgo, Nuria Matesanz, Magdalena Leiva, Guadalupe Sabio
Article
Pathology
Keren Grynberg, Elyce Ozols, William R. Mulley, Roger J. Davis, Richard A. Flavell, David J. Nikolic-Paterson, Frank Y. Ma
Summary: The JNK1 pathway plays a specific role in IRI-induced proximal tubule cell death, leading to acute renal failure. Jnk1 deficient mice and conditional Jnk1 deletion in the proximal tubule showed significant protection against acute renal ischemia/reperfusion injury. JNK2 does not have a similar role in this process.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Katrin Mueller, Hanna Honcharova-Biletska, Christiane Koppe, Michele Egger, Lap Kwan Chan, Anne T. Schneider, Lena Kuesgens, Friederike Boehm, Yannick Boege, Marc E. Healy, Johannes Schmitt, Sarah Comtesse, Mirco Castoldi, Christian Preisinger, Marta Szydlowska, Enrico Focaccia, Nadine T. Gaisa, Sven H. Loosen, Simone Joers, Frank Tacke, Christoph Roderburg, Verena Keitel, Johannes G. Bode, Peter Boor, Roger J. Davis, Thomas Longerich, Fabian Geisler, Mathias Heikenwalder, Achim Weber, Mihael Vucur, Tom Luedde
Summary: Experimental studies revealed the role of the JNK signaling pathway in regulating liver homeostasis during aging, conditional ablation of JNK1 and JNK2 may promote age-related cyst development, which is associated with RIPK1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Amada D. Caliz, Hyung-Jin Yoo, Anastassiia Vertii, Ana C. Dolan, Cathy Tournier, Roger J. Davis, John F. Keaney, Shashi Kant
Summary: MKK4 and MKK7, members of the MAP2K family, can activate downstream MAPKs, with MKK7 playing a crucial role in JNK activation in both in vitro and in vivo settings. MKK7 is necessary for LPS-induced cytokine production, M1 polarization, and migration in macrophages, contributing significantly to the inflammatory response. On the other hand, MKK4 plays a significant, but minor role in cytokine production in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, General & Internal
Deepak K. Singh, Vaibhav G. Patel, William K. Oh, Julio A. Aguirre-Ghiso
Summary: Prostate cancer has a variable clinical course, with possibilities ranging from curable local disease to lethal metastatic spread. The dynamic interplay between dormant disseminated cancer cells (DCCs) and the primary tumor microenvironment (TME) as well as the secondary organ niche plays a crucial role in controlling the conversion between states of dormancy and activation.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Medicine, Research & Experimental
Timothy D. Calamaras, Suchita Pande, Robert A. U. Baumgartner, Seung Kyum Kim, Joseph C. McCarthy, Gregory L. Martin, Kelly Tam, Angela L. McLaughlin, Guang-rong Wang, Mark J. Aronovitz, Weiyu Lin, Jonathan I. Aguirre, Paulina Baca, Peiwen Liu, Daniel A. Richards, Roger J. Davis, Richard H. Karas, Iris Z. Jaffe, Robert M. Blanton
Summary: PKG1 alpha plays a role in promoting left ventricle compensation after pressure overload. MLK3 acts as a substrate of PKG1 alpha, mediating its protective effects on LV function without affecting acute blood pressure responses. Interestingly, MLK3 kinase-dependent effects preserve LV function, while kinase-independent signaling regulates blood pressure.
Article
Biochemistry & Molecular Biology
Ekin Guney, Ana Paula Arruda, Gunes Parlakgul, Erika Cagampan, Nina Min, Yankun Lee, Lily Greene, Eva Tsaousidou, Karen Inouye, Myoung Sook Han, Roger J. Davis, Gokhan S. Hotamisligil
Summary: Chronic metabolic inflammation, obesity, and diabetes are closely related to altered regulation of the Ca2+ channel inositol trisphosphate receptor (IP3R) in adipocytes. Studies in mice have shown that adipocyte-specific loss of IP3R1/2 protects against adipose tissue inflammation and insulin resistance, indicating that targeting IP3R-mediated Ca2+ homeostasis in adipocytes could be a potential therapeutic approach for metabolic diseases.
Article
Genetics & Heredity
Abigail O. Smith, Julie A. Jonassen, Kenley M. Preval, Roger J. Davis, Gregory J. Pazour
Summary: Polycystic kidney disease is a genetic disorder caused by mutations in Pkd1 and Pkd2. Research indicates that targeting the JNK pathway may hold promise as a potential therapeutic approach for cystic kidney disease.
Article
Oncology
Chaobo Chen, Hanghang Wu, Hui Ye, Agustin Tortajada, Sandra Rodriguez-Perales, Raul Torres-Ruiz, August Vidal, Maria Isabel Peligros, Johanna Reissing, Tony Bruns, Mohamed Ramadan Mohamed, Kang Zheng, Amaia Lujambio, Maria J. Iraburu, Leticia Colyn, Maria Ujue Latasa, Maria Arechederra, Maite G. Fernandez-Barrena, Carmen Berasain, Javier Vaquero, Rafael Banares, Leonard J. Nelson, Christian Trautwein, Roger J. Davis, Eduardo Martinez-Naves, Yulia A. Nevzorova, Alberto Villanueva, Matias A. Avila, Francisco Javier Cubero
Summary: This study reveals the contribution of unfolded protein response activation and liver tissue scarring to the development of polycystic liver disease (PLD) and early cancer. The mutant mice used in this study serve as an ideal tool for investigating PLD and early cancer development. The findings suggest that activation of unfolded protein response and fibrosis may trigger the formation of hepatic cysts and early stages of cholangiocarcinoma (CCA).
Article
Cell Biology
G. Kenneth Gray, Carman Man-Chung Li, Jennifer M. Rosenbluth, Laura M. Selfors, Nomeda Girnius, Jia-Ren Lin, Ron C. J. Schackmann, Walter L. Goh, Kaitlin Moore, Hana K. Shapiro, Shaolin Mei, Kurt D'Andrea, Katherine L. Nathanson, Peter K. Sorger, Sandro Santagata, Aviv Regev, Judy E. Garber, Deborah A. Dillon, Joan S. Brugge
Summary: This study presents a high-resolution breast atlas by integrating various techniques, defining different types of breast cells and their associations with breast cancer risk factors. A subset of cells associated with basal-like breast cancer was identified, and molecular factors regulating cell proportions were identified.
DEVELOPMENTAL CELL
(2022)
Article
Cell Biology
G. Kenneth Gray, Nomeda Girnius, Hendrik J. Kuiken, Aylin Z. Henstridge, Joan S. Brugge
Summary: This study uses mass cytometry and cyclic immunofluorescence to reveal the heterogeneity of murine mammary epithelial cells and uncover the connection between endocrine regulation and lineage suppression.
Article
Oncology
Julie S. Di Martino, Ana Rita Nobre, Chandrani Mondal, Isra Taha, Eduardo F. Farias, Elana J. Fertig, Alexandra Naba, Julio A. Aguirre-Ghiso, Jose Javier Bravo-Cordero
Summary: Cancer cells disseminate and seed in distant organs, remaining dormant before forming detectable metastases. Research shows that dormant cancer cells assemble an ECM niche enriched with type III collagen, which is essential for sustaining tumor dormancy. Disruption of tumor-derived type III collagen can restore tumor cell proliferation and lead to dormancy-to-reactivation transition.
Article
Gastroenterology & Hepatology
Francisco Javier Cubero, Mohamed Ramadan Mohamed, Marius M. Woitok, Gang Zhao, Maximilian Hatting, Yulia A. Nevzorova, Chaobo Chen, Johannes Haybaeck, Alain de Bruin, Matias A. Avila, Mark Boekschoten, Roger J. Davis, Christian Trautwein
HEPATOLOGY COMMUNICATIONS
(2020)