Review
Cell Biology
Begona Canovas, Angel R. Nebreda
Summary: The ability of cells to cope with various stressful situations is crucial for survival, with p38 alpha kinase playing a key role in the cellular stress response. Understanding the diversity of p38 alpha substrates, their mechanisms, and their connections to specific cellular functions is important for insights into physiology and pathology. Dysregulation of the p38 alpha pathway has been linked to diseases such as inflammation, immune disorders, and cancer.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Mengyi Lao, Xiaozhen Zhang, Hanshen Yang, Xueli Bai, Tingbo Liang
Summary: This review highlights the crucial role of regulator of calcineurin 1 (RCAN1) in the pathogenesis of cancer and its interaction with calcineurin. High expression of RCAN1 inhibits tumor progression, except for hypopharyngeal and laryngopharynx cancer. Therefore, targeting RCAN1 may be a promising therapeutic approach for cancer treatment.
MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Juan R. Perea, Esther Garcia, Laura Valles-Saiz, Raquel Cuadros, Felix Hernandez, Marta Bolos, Jesus Avila
Summary: Tauopathies are neurodegenerative diseases characterized by the accumulation of hyperphosphorylated tau protein. Inflammation, particularly the activation of microglia, is also involved in these diseases. The p38 MAPK pathway, primarily expressed in glia, has been associated with tau phosphorylation in neurodegenerative diseases like Alzheimer's Disease. Using a mouse model, researchers found increased p38 activation in microglia of the hippocampus during aging. Interestingly, these mice also displayed activated rod microglia, although p38 activation was decreased in this subpopulation. This suggests that rod microglia may have a neuroprotective phenotype in the context of tau pathology.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Enkhtuya Radnaa, Lauren Richardson, Brett Goldman, Jared K. Burks, Tuvshintugs Baljinnyam, Natasha Vora, Hui-Juan Zhang, Elizabeth A. Bonney, Arum Han, Ramkumar Menon
Summary: This study investigated the impact of oxidative stress on p38 MAPK signaling and cell fate, using amnion epithelial cells. The results showed that excessive activation of p38 can have adverse effects on cell behavior and tissue homeostasis.
Article
Pharmacology & Pharmacy
Xiang Huang, Ziheng Wang, Mengruo Song, He Huan, Zishu Cai, Bing Wu, Jianhong Shen, You Lang Zhou, Jinlong Shi
Summary: In this study, the expression of circRNA (circIQGAP1) was found to be significantly downregulated in IDH1 mutant gliomas, and low circIQGAP1 expression was associated with better prognosis. circIQGAP1 regulated glioma cell behaviors through miR-1256/RCAN1/Bax/Bcl-2/Caspase3 and miR-622/RCAN2/Bax/Bcl-2/Caspase3 axes. The findings suggest that circIQGAP1 plays an important role in glioma development and may serve as a therapeutic target.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Olivia Molinar-Inglis, Jacob M. Wozniak, Neil J. Grimsey, Lennis B. Orduna-Castillo, Norton Cheng, Ying Lin, Monica L. Gonzalez Ramirez, Cierra A. Birch, John D. Lapek, David J. Gonzalez, JoAnn Trejo
Summary: This study identified candidate proteins regulated by PAR1-p38 signaling that may play important roles in endothelial dysfunction, by analyzing the global phosphoproteome in human cultured endothelial cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yue Zhou, Ryota Oki, Akihiro Tanaka, Leixin Song, Atsushi Takashima, Naru Hamada, Satoru Yokoyama, Seiji Yano, Hiroaki Sakurai
Summary: Under cellular stress conditions, non-canonical EphA2 activation occurs through the p38-MK2-RSK pathway and promotes glioblastoma cell migration.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Cell Biology
Christopher M. Brennan, Abby S. Hill, Michael St Andre, Xianfeng Li, Vijaya Madeti, Susanne Breitkopf, Seth Garren, Liang Xue, Tamara Gilbert, Angela Hadjipanayis, Mara Monetti, Charles P. Emerson Jr, Robert Moccia, Jane Owens, Nicolas Christoforou
Summary: This study investigated the kinetics of DUX4-induced stresses and found that the JNK and p38 MAP kinase pathways are activated in DUX4-mediated FSHD, providing further insights into potential therapeutic targets.
DISEASE MODELS & MECHANISMS
(2022)
Article
Chemistry, Medicinal
Yun Wu, Ziping Qi, Beilei Wang, Junjie Wang, Qingwang Liu, Aoli Wang, Chenliang Shi, Bin Zhou, Qianmao Liang, Wenliang Wang, Fengming Zou, Shuang Qi, Zuowei Wang, Li Wang, Wenchao Wang, Jing Liu, Qingsong Liu
Summary: A novel, potent, and selective MST1 inhibitor 19 was discovered, which protected ss cells from inflammatory cytokines in vitro and showed potential in treating diabetes in animal models.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Jeremy C. Burton, Jennifer Okalova, Neil J. Grimsey
Summary: In this study, the spatiotemporal dynamics of p38 activity were investigated using targeted fluorescence resonance energy transfer (FRET) p38 activity biosensors. The results showed that p38 activation through the MKK3/6 pathway exhibited enhanced activity in the nucleus, while p38 activation via the thrombin-mediated PAR1 pathway showed increased activity in the endosome and cytosol, limiting nuclear p38 activity.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Miljan Kuljanin, Dylan C. Mitchell, Devin K. Schweppe, Ajami S. Gikandi, David P. Nusinow, Nathan J. Bulloch, Ekaterina V. Vinogradova, David L. Wilson, Eric T. Kool, Joseph D. Mancias, Benjamin F. Cravatt, Steven P. Gygi
Summary: This study presents a redesigned workflow called streamlined cysteine activity-based protein profiling (SLC-ABPP) for measuring amino acid side-chain reactivity, which significantly improves sample throughput. The method has been successfully applied to identify proteome-wide targets of various covalent inhibitors, while also creating a resource of cysteine reactivity data for further research.
NATURE BIOTECHNOLOGY
(2021)
Article
Cell Biology
Gordana Scepanovic, Miranda Victoria Hunter, Ran Kafri, Rodrigo Fernandez-Gonzalez
Summary: The research demonstrates that in Drosophila embryos, activation of p38 kinase helps limit wound size and promote rapid wound healing by increasing cell volume around the wound.
Article
Biology
Constantinos G. Broustas, Sanjay Mukherjee, Evan L. Pannkuk, Evagelia C. Laiakis, Albert J. Fornace, Sally A. Amundson
Summary: Radiation biodosimetry based on transcriptomic analysis of peripheral blood is a valuable tool for detecting radiation exposure, but confounding factors may affect its predictive power. The p38 signaling pathway plays an important role in the response to radiation, and its attenuation may protect blood cells from radiation-induced damage.
RADIATION RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Linyao Zhao, Yixuan Wang, Yang Xu, Qian Sun, Hao Liu, Qianxue Chen, Baohui Liu
Summary: The study demonstrated that BIRB796 inhibits proliferation and invasion in GBM cells, suggesting its potential as an adjuvant therapy to enhance the efficacy of GBM treatment.
Article
Biology
Michal Otreba, Johanna Johansson Sjolander, Morten Grotli, Per Sunnerhagen
Summary: The small molecule INR119 enhances the activity of protein kinases in fission yeast and human cells under oxidative stress, with a potential conservation of its mechanism of action between yeast and humans.
Article
Genetics & Heredity
Songbiao Zhu, Wenxi Ding, Yuling Chen, Weixuan Wang, Renhua Xu, Chongdong Liu, Xiaohui Liu, Haiteng Deng
Summary: High expression of VHL is correlated with better disease-free survival in ccRCC patients. VHL overexpression inhibits EMT, triglyceride synthesis, and cell proliferation in ccRCC cells, and renders them more sensitive to interferon treatment.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Letter
Cell Biology
Shuyuan Zhang, Qingtai Liang, Xinheng He, Chongchong Zhao, Wenlin Ren, Ziqing Yang, Ziyi Wang, Qiang Ding, Haiteng Deng, Tong Wang, Linqi Zhang, Xinquan Wang
Article
Biochemistry & Molecular Biology
Chengting Luo, Changmei Yang, Xueying Wang, Yuling Chen, Xiaohui Liu, Haiteng Deng
Summary: Research showed that nicotinamide supplementation can reduce fat mass in obese mice, improve glucose tolerance, and enhance mitochondrial function in adipose tissue. Nicotinamide activates NAMPT and increases cellular NAD(+) levels to reprogram cellular metabolism for better adipose tissue function.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Yingying Ma, Songbiao Zhu, Meiqi Yi, Wenhao Zhang, Yuanyuan Xue, Xiaohui Liu, Haiteng Deng
Summary: Protein S-glutathionylation is an important post-translational modification that regulates cellular processes. This study investigated the changes in glutathionylome during epithelial-mesenchymal transition (EMT) and found that the glutathionylation level of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) increased, inhibiting its enzymatic activity and promoting EMT.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yaqi Chen, Sha Zhou, Kairui Wan, Long Yu, Chongchong Zhao, Haiteng Deng, Qingjian Ou, Jiayi Qin, Junbo Hu, Zhenlin Hou
Summary: RIO kinase 1 (RIOK1) plays an important role in the radioresistance of colorectal cancer (CRC) by suppressing the p53 signaling pathway. Targeting RIOK1 may alleviate the CRC tumor burden in patients. This study also revealed the interaction between RIOK1 and G3BP2, and the phosphorylation of G3BP2 by RIOK1 enhances the ubiquitination of p53.
Review
Cell Biology
Weixuan Wang, Changmei Yang, Tianxiang Wang, Haiteng Deng
Summary: Nicotinamide N-methyltransferase (NNMT) plays critical roles in cancer progression by affecting cellular methylation reactions and signaling pathways, and it has been identified as a potential biomarker and therapeutic target for tumor diagnosis and treatment.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Chengting Luo, Wenxi Ding, Songbiao Zhu, Yuling Chen, Xiaohui Liu, Haiteng Deng
Summary: It has been reported that the activities of NAD(+)-dependent deacetylase decrease in the aging mouse liver, while NMN treatment can increase healthspans. However, the changes in the acetylomic landscape and the effects of NMN treatment on protein acetylation in the aging liver have not been studied. In this study, immunoprecipitation coupled with label-free quantitative LC-MS/MS was used to identify the acetylome and investigate the effects of aging and NMN on liver protein acetylation. The results showed that the acetylation levels of proteins involved in oxidation-reduction and metabolic processes increased in aged livers, while the acetylation levels of proteins involved in transport and translation processes decreased. NMN treatment inhibited the age-related increase in acetylation of proteins regulating fatty acid beta oxidation, the TCA cycle and valine degradation.
Article
Biochemical Research Methods
Chengting Luo, Wenxi Ding, Changmei Yang, Wenhao Zhang, Xiaohui Liu, Haiteng Deng
Summary: In this study, the researchers found that the oxidation-reduction process was associated with aging in the mouse liver. They also discovered that oxidative stress activates Nrf2 and its downstream genes, but supplementation of NMN reduced oxidative stress and restored adaptive homeostasis through the Sirt3-Nrf2 axis.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Shengli Zhuang, Dong Chen, Wentao Fan, Jinyun Yuan, Lingwen Liao, Yan Zhao, Jin Li, Haiteng Deng, Jun Yang, Jinlong Yang, Zhikun Wu
Summary: This study proposes the concept of single-atom-kernelled nanoclusters and synthesizes a Pd-based trimetal nanocluster with a single-Ag atom-kernel using an alloying strategy. The nanoclusters with single-silver atom kernels exhibit higher activity and selectivity compared to the bimetal precursor nanoclusters. The single atoms in the kernel have significant influence on catalytic performance by affecting the electronic configuration.
Article
Nanoscience & Nanotechnology
Jun Zha, Xiangfu Meng, Wentao Fan, Qing You, Nan Xia, Wanmiao Gu, Yan Zhao, Lin Hu, Jin Li, Haiteng Deng, Hui Wang, Nan Yan, Zhikun Wu
Summary: In this study, surface site specific antigalvanic replacement with remaining composition/structure and surface replacement-dependent selectivity in the electrocatalytic reduction of CO2 was achieved for the first time. Density functional theory calculations explained the catalysis selectivity switch induced by replacing Ag with Cu and the facilitation of C2 production by Cu replacement. Furthermore, CO2 electroreduction to C2 on well-defined metal nanoclusters was reported for the first time in this study.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Physical
Wentao Fan, Ying Yang, Qing You, Jin Li, Haiteng Deng, Nan Yan, Zhikun Wu
Summary: There are unresolved fundamental issues regarding metal nanoclusters, including the transformation from face-centered cubic (fcc) to hexagonal close-packed (hcp) structures and photoexcitation electron transfer. This study successfully achieved the fcc-to-hcp structure transformation and revealed size and shape-dependent photoexcitation electron transfer for metal nanoclusters. A new thermally induced ligand exchange method was developed to synthesize a rod-shaped hcp Au42(SCH2Ph)32 nanocluster, which exhibited specific absorption and dual emission properties. The findings also suggest a possible structure transformation pathway and predict a novel rod-shaped hcp Au58(SR)40 nanocluster with a larger aspect ratio. Additionally, a kernel-based middle-to-both ends sp <- sp photoexcitation electron transfer mechanism was observed in rod-like gold nanoclusters, indicating the potential for tunable and practical applications.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Biochemical Research Methods
Xueying Wang, Chengting Luo, Lina Xu, Yusong Wang, Lv Jun Guo, Yupei Jiao, Haiteng Deng, Xiaohui Liu
Summary: Isotope tracing assisted metabolic analysis is a useful tool to understand metabolic regulation. This study presents a strategy called pseudo-targeted profiling of isotopic metabolomics (PtPIM) to expand the analysis of isotope labeled metabolites. The PtPIM method successfully broadened metabolite coverage in isotope tracing analysis and revealed the effects of rotenone on mitochondrial function.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Zhiwen Zhu, Dongdong Li, Zeran Jia, Wenhao Zhang, Yuling Chen, Ruixue Zhao, Yan-Ping Zhang, Wen-Hong Zhang, Haiteng Deng, Yinqing Li, Wei Li, Shouhong Guang, Guangshuo Ou
Summary: Eukaryotic organisms adapt to environmental fluctuations by altering their epigenomic landscapes and transcriptional programs. Histone H2Bs are globally degraded in Caenorhabditis elegans during starvation, and the mechanisms underlying this degradation involve ubiquitin and ubiquitin-related enzymes. The retention of aberrant nucleosomal H2Bs leads to abnormal gene expression and impacts animal viability. Furthermore, the ubiquitin-proteasome system regulates chromosomal histone turnover in human cells.
Article
Multidisciplinary Sciences
Qian Gong, Yunjing Wang, Linfang He, Fan Huang, Danfeng Zhang, Yan Wang, Xiang Wei, Meng Han, Haiteng Deng, Lan Luo, Feng Cui, Yiguo Hong, Yule Liu
Summary: This study reveals the mechanism of airborne defense (AD) against aphids and viruses. The volatile compound MeSA is perceived by neighboring plants and converted into salicylic acid, which activates a signaling cascade to induce plant anti-aphid immunity and reduce virus transmission. Aphid-transmitted viruses encode proteins that suppress AD, making plants less repellent to aphids and more suitable for aphid survival, infestation, and viral transmission.
Article
Biochemistry & Molecular Biology
Huaijin Zhang, Yuling Chen, Xiaohui Liu, Haiteng Deng
Summary: This study found that mitochondrial dysfunction, rotenone treatment, elevated Ca2+ levels, and activation of the JNK-STAT3 pathway all contribute to increased resistance of glioblastoma to TMZ. Inhibiting JNK or STAT3 can increase sensitivity to TMZ. Co-administration of TMZ with a JNK or STAT3 inhibitor holds promise as an effective treatment for glioblastoma.
