Article
Multidisciplinary Sciences
Xue-Fei Zhou
Summary: In this study, laccase was immobilized into different carriers and comparative studies were conducted on phenolic and nonphenolic lignin model compounds in the presence of ABTS. The graphene oxide immobilized laccase showed better performance in terms of conversion rate and reusability. Characterization of structural modifications induced in organosolv lignin was carried out using GPC and P-31-NMR, revealing higher reactivity and selectivity of the graphene oxide-immobilized laccase compared to molecular sieves.
CHIANG MAI JOURNAL OF SCIENCE
(2021)
Article
Engineering, Environmental
Lin Hu, Xian-Yong Wei, Mei-Ling Xu, Yu-Hong Kang, Xian-Hou Guo, Feng-Bin Zhang, Zhi-Min Zong, Hong-Cun Bai
Summary: This research demonstrates that Pd-based zeolite catalysts show high catalytic activity in the HDO conversion of Guaiacol, resulting in high yield of hydrocarbons.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Physical
Laura Karina Escalante Morales, Prakhar Sengar, Andrea Dorado Baeza, Rafael Vazquez-Duhalt, Kanchan Chauhan
Summary: Highly stable and reusable magnetic crosslinked enzyme aggregates (m-CLEAS) of laccase were synthesized with improved enzymatic activity. The aggregates were formed by co-aggregation of enzyme with magnetic copper ferrite nanoparticles (CFNPs) and crosslinked using glutaraldehyde. The m-CLEAS showed higher transformation rate and catalytic efficiency compared to the free enzyme. They also exhibited superior storage stability and recyclability. This study highlights the easy and cost-effective synthesis of nanometric m-CLEAS with improved laccase activity, making them suitable for various green industrial processes.
Article
Chemistry, Multidisciplinary
Hainan Yu, Bijia Wang, Yamei Wang, Enhui Xu, Rong Wang, Shouying Wu, Wei Wu, Bolin Ji, Xueling Feng, Hong Xu, Yi Zhong, Zhiping Mao
Summary: We report the synthesis of highly phenolic lignin nanoparticles (HP-LNPs) through alkaline treatments and enzymatic treatment using laccase. Urea and H2O2 were used to enhance the antioxidant properties and reduce visible light absorption of the LNPs. The HP-LNPs exhibited excellent antioxidant properties and increased shelf life of fruits.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Deepti Yadav, Bibhuti Ranjan, Nokuthula Mchunu, Marilize Le Roes-Hill, Tukayi Kudanga
Summary: In this study, magnetic nanoparticles were successfully synthesized and functionalized by silanization for the covalent immobilization of a recombinant small laccase. The immobilized enzyme showed efficient degradation of phenolic compounds in the presence of a natural mediator, good stability, and enhanced catalytic activity with selected metal ions.
Article
Engineering, Environmental
Yeonjoon Kim, Mohammed A. Jabed, David M. Price, Dmitri Kilin, Seonah Kim
Summary: This study provides rational design guidelines for supported V2O5 catalysts in the catalytic upgrading of lignocellulosic biomass, particularly in the conversion of guaiacol to phenol. The tilt angle of the aryl group in the hydrogen migration transition state is identified as a key descriptor in determining the catalytic activity of phenol formation.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Environmental Sciences
Zhonghao Chen, Wen-Da Oh, Pow-Seng Yap
Summary: Phenolic compounds are highly detrimental to human and living beings. The development of suitable remediation technologies is critically important. Utilizing enzymatic technologies for biodegradation is a promising solution to address pollution caused by phenolic compounds in aquatic environments. This review presents the latest developments in using immobilized laccase for the degradation of phenolic compounds in water.
Article
Agricultural Engineering
Carlos A. Pena, Lina F. Ballesteros, Hector Rodriguez, Eva Rodil, Jose A. Teixeira, Michele Michelin
Summary: This study investigates the valorisation of lignin in a biorefinery based on lignocellulosic biomass by treating technical lignin with recombinant laccase and mediator. The treatment resulted in a preferential reduction of guaiacyl units in the recovered lignin. Phenolic compounds deriving from guaiacyl or p-hydroxyphenyl units were identified in the aqueous phases, with higher concentrations observed when an ionic liquid was involved in the treatment.
BIOMASS & BIOENERGY
(2023)
Article
Chemistry, Analytical
Liu Yang, Xiao-Yun Guo, Qiong-Hua Zheng, Yin Zhang, Ling Yao, Qiu-Xia Xu, Jin-Cheng Chen, Shao-Bin He, Wei Chen
Summary: In this study, platinum nanoparticles were constructed as a laccase-like nanozyme, which exhibited higher substrate affinity and catalytic effect compared to natural laccase. The nanozyme also demonstrated high stability at a wide range of pH and temperature. Additionally, it efficiently detected laccase-related phenolic compounds and showed potential applications in nanozyme research and environmental remediation.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Norah Salem Alsaiari, Abdelfattah Amari, Khadijah Mohammedsaleh Katubi, Fatimah Mohammed Alzahrani, Hamed N. Harharah, Faouzi Ben Rebah, Mohamed A. Tahoon
Summary: In this study, free laccase was immobilized on magnetic iron oxide and copper ferrite nanoparticles to improve its catalytic activity and stability. The immobilized laccase showed enhanced activity compared to free laccase, especially with CuFe2O4 nanoparticles as support. The nanobiocatalysts were also effective in degrading the anionic dye DR23, with good reusability and stability demonstrated through multiple cycles of degradation.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Qian-qian Yang, Wen-ping Hua, Hao-lan Zou, Jia-xin Yang, Xiang-zeng Wang, Tong Zhang, Dong-hao Wang, Xiao-jia Zhu, Xiao-yan Cao
Summary: This study investigated the expression patterns and functionality of SmLAC25 in Salvia miltiorrhiza. The results showed that overexpression of SmLAC25 increased lignin content in plantlets and hairy roots, but decreased the concentrations of rosmarinic acid and salvianolic acid B. Further studies revealed that the overexpression of SmLAC25 promoted lignin synthesis and inhibited salvianolic acid biosynthesis.
Article
Engineering, Chemical
Lei Zhang, Yuanyuan Feng, Linlin Li, Xiaofang Guo, Wei Du, Songbo Wang, Jun Xiang, Penggao Cheng, Na Tang
Summary: Novel Fe-enzyme complexes were fabricated with magnetic nanoparticles embedded in/on enzymes in situ, serving as efficient catalysts for high-efficiency catalysis and separation. The complexes, mainly composed of enzymes and MNPs, can be separated and collected by a magnetic field after catalytic reaction, with the preparation process controllable under mild conditions to retain enzyme activity. This approach may provide a facile and general way to prepare MNP-biomacromolecule complexes with efficient load.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Runtang Liu, Wei Zhang, Shushu Wang, Huajin Xu, Yi Hu
Summary: In this study, a highly efficient strategy for laccase immobilization to degrade phenolic pollutants was developed by combining polyethyleneimine with magnetic Fe3O4 nanoparticles and chelating laccase onto the carrier through Cu2+ chelation. The carrier demonstrated excellent immobilization ability and high activity retention. It showed improved thermal stability and storage stability compared to free laccase. The immobilized laccase also exhibited a good removal effect on various phenolic pollutants.
Article
Agricultural Engineering
Sandra Gonzalez-Rodriguez, Thelmo A. Lu-Chau, Xinyi Chen, Gemma Eibes, Antonio Pizzi, Gumersindo Feijoo, Maria Teresa Moreira
Summary: Bio-based adhesives provide a sustainable alternative to formaldehyde-based adhesives. The low reactivity of lignin for the synthesis of bio-based adhesives is a major challenge. The use of laccases to demethylate organosolv lignin showed promising results, and optimal conditions were determined by the use of surfactants and mediators. Factors such as pH, temperature, and aeration also affected the enzymatic demethylation of lignin.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Engineering, Environmental
Dan Wang, Jiangfei Lou, Jiugang Yuan, Jin Xu, Ruofei Zhu, Qiang Wang, Xuerong Fan
Summary: Amino-functionalized magnetic core-shell structure metal-organic framework microspheres were successfully constructed and employed for Trametes versicolor laccase immobilization, showing excellent catalytic performance and stability. The immobilized laccase demonstrated high efficiency in removing environmental pollutants, indicating significant potential for practical applications in wastewater treatment.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
