Article
Biochemistry & Molecular Biology
Meiling Zhang, Seung-Hyun Jun, Youngho Wee, Han Sol Kim, Ee Taek Hwang, Jongmin Shim, Sang Youn Hwang, Jinwoo Lee, Jungbae Kim
Summary: In this study, a nanoscale enzyme reactor (NER) for lipase from Rhizopus oryzae was prepared, which showed high stability and activity. The NER demonstrated great potential as an environmentally-friendly nanobiocatalyst for various lipase applications, including biodiesel production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Energy & Fuels
Fahimeh Esmi, Tahereh Nematian, Zeinab Salehi, Abbas Ali Khodadadi, Ajay K. Dalai
Summary: Fatty acid methyl ester (FAME) was synthesized from olive oil using Rhizopus oryzae lipase (ROL) immobilized on mesoporous silica magnetic nanoparticles (MNPs). The immobilized lipase showed increased enzyme loading efficiency and led to a higher biodiesel production compared to other nanobiocatalysts, making it a suitable nanomaterial for ROL immobilization. The physico-chemical properties of the MNPs were characterized and correlated with their activities for biodiesel synthesis.
Article
Agriculture, Multidisciplinary
Liu-Jia Xu, Tao Yang, Jing Wang, Feng-Hong Huang, Ming-Ming Zheng
Summary: Efficient enzymatic synthesis of resveratrol ester derivatives were achieved by immobilized lipase on HMSS-C-8, with high catalytic efficiency and conversion rate. The research provides a new pathway for the application of resveratrol monoester derivatives as excellent lipid-soluble antioxidants.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Priyanka V. Jawale, Bhalchandra M. Bhanage
Summary: The study focused on analyzing reaction kinetics and mechanism for synthesizing propyl benzoate in solvent-free conditions, immobilizing lipase on HPMC and PVA polymer blend, with CCL showing excellent activity. Optimizing reaction conditions and calculating kinetic parameters revealed that propyl benzoate synthesis using immobilized CCL followed the ternary complex model.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Review
Energy & Fuels
Mohsin Raza, Labeeb Ali, Abrar Inayat, Lisandra Rocha-Meneses, Shams Forruque Ahmed, Md Mofijur, Farrukh Jamil, Chukwuma Leonard Azimoh
Summary: Biodiesel is a sustainable energy substitute with environmentally friendly characteristics. Immobilized-enzyme technology improves the stability, reactivity, and reusability of biodiesel production. The potential and importance of immobilized-enzyme technology in biodiesel production are significant.
INTERNATIONAL JOURNAL OF ENERGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Misun Kang, Jong-tak Lee, Jae Young Bae
Summary: Formaldehyde emitted from household products is harmful to human health. This study compares the formaldehyde adsorption characteristics of mesoporous and mesoporous hollow silicas synthesized with or without a calcination process. The mesoporous hollow silica synthesized without a calcination process has the best adsorption performance, followed by the mesoporous hollow silica synthesized with a calcination process and mesoporous silica. This research demonstrates the potential of mesoporous hollow silica as an effective support for harmful gas adsorption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Kholoud El-Kady, Mai Raslan, Ayman H. Zaki
Summary: In this study, the immobilization of lipase from Aspergillus niger on TiO2 nanoparticles with different morphologies was investigated. The lipase immobilized on TiO2 microspheres showed the highest activity, while the hydrolytic activity was found to be the highest at a microsphere/lipase ratio of 1:0.25. Additionally, the immobilization yield activities were higher for lower microsphere/lipase ratios.
Article
Chemistry, Multidisciplinary
Jennifer Noro, Joana Cabo, David S. Freitas, Catarina S. Roque, Mariana de Castro, Artur Cavaco-Paulo, Carla Silva
Summary: In this study, three deep eutectic mixtures (DES) were used as mediums to synthesize glucose laurate and glucose acetate. Lipases from Aspergillus oryzae (LAO), Candida rugosa (LCR), and porcine pancreas (LPP) were employed as catalysts. The results showed that DES did not deactivate the lipases when used as medium. For transesterification reactions, combining LAO or LCR with DES 3 resulted in efficient production of glucose laurate, while LPP performed best in DES 2 for glucose acetate synthesis. Substituting vinyl laurate with vinyl acetate led to different behaviors, with LCR and LPP showing better catalytic activity in DES 1 for glucose acetate production. LAO had lower activity, with only around 40% product yield in DES 3. These findings highlight the potential of biocatalysis combined with greener solvents for the synthesis of differentiated sugar fatty acid esters.
Article
Chemistry, Inorganic & Nuclear
Wanlu Liu, Chunhui Liu, Luca Pilia, Hongliang Zhang, Pascal van der Voort, Anna M. Kaczmarek, Rik Van Deun
Summary: We synthesized a co-condensed hollow ethane-bipyridine periodic mesoporous organosilica (HEt-bpy-PMO) as a host material to anchor lanthanides for developing a multifunctional chemical sensor. The luminescence properties of the developed hybrid materials were studied in detail in the solid-state and after dispersing in water. The Eu3+ or Tb3+ incorporated materials showed ions selectivity towards Cu2+, Co2+, and Fe3+ as ion sensors, and exhibited excellent acetone sensing selectivity.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Multidisciplinary
Rebecca Hollenbach, Andre Delavault, Laura Gebhardt, Hannah Soergel, Claudia Muhle-Goll, Katrin Ochsenreither, Christoph Syldatk
Summary: In this study, the combination of biocatalysis and mechanochemistry was used to synthesize glycolipid surfactants, resulting in higher yields using unconventional dissolved reaction systems and bead mill apparatus.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Natarajan Yasvanthrajan, Pandian Sivakumar, Karuppan Muthukumar, Thanabalan Murugesan, Appusamy Arunagiri
Summary: This study demonstrates process intensification through lipase-catalyzed transesterification of waste cottonseed oil with ethanol under ultrasonication, achieving a maximum conversion rate of 98.7%. The optimized process conditions include an ethanol to oil molar ratio of 4.5:1, a reaction temperature of 45 degrees C, enzyme loading of 5 wt%, a reaction time of 6 h, ultrasonic amplitude of 40%, and a duty cycle of 15 s ON and 15 s OFF. The use of ultrasound enhances efficiency by reducing reaction time to 6 hours with minimal catalyst dosage and power consumption.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Biochemistry & Molecular Biology
Xiaonan Zhou, Wang Zhang, Linwei Zhao, Shan Gao, Tianyi Liu, Dianyu Yu
Summary: In this study, carboxyl-modified mesoporous silica MCM-41 was assembled with chitosan to create a novel nanocarrier for the immobilization of lipase. The composite was further modified with silane coupling agent KH560 to decrease pore size and prevent enzyme loss. The lipase fixed in the CTS-MCM-41 carrier showed a residual activity of 85% after seven repeated use cycles. The optimal conditions for enzyme immobilization involved a carrier to enzyme mass ratio of 4:1, 3% glutaraldehyde solution volume, 3-hour reaction time, and 45°C reaction temperature. Overall, the proposed innovative carrier allows for stable immobilization of lipase and offers potential for large-scale industrial production in an efficient miniature reactor.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Energy & Fuels
Ehsan Parandi, Maryam Safaripour, Magda H. Abdellattif, Majid Saidi, Alireza Bozorgian, Hamid Rashidi Nodeh, Shahabaldin Rezania
Summary: The main objective of this study was to produce biodiesel from waste cooking oil using immobilized lipase on a magnetic nanocomposite. The synthesized biocatalyst showed high efficiency in producing biodiesel at lower temperatures and optimal reaction conditions.
Article
Chemistry, Analytical
Hong Wang, Yuehao Fu, Xiaohua Liu, Ruisong Yang, Yu Hu, Di Liu, Jiawei Wan, Zhipeng Zeng
Summary: The snowball flower-like g-C3N4/ZnFe2O4 mesoporous hollow microspheres, composed of 2D g-C3N4 nanolayers loaded onto the surface of ZnFe2O4 hollow microspheres, were successfully synthesized by a simple solid phase reaction. The concentration of g-C3N4 was found to affect the structure, morphology, and gas-sensing performance of the g-C3N4/ZnFe2O4 composites. The sensor based on g-C3N4/ZnFe2O4-15 hollow microspheres exhibited excellent sensing properties for TEA, including high response, fast response-recovery time, and outstanding stability.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Green & Sustainable Science & Technology
Rahmath Abdulla, Eryati Derman, Thivyasri K. Mathialagan, Abu Zahrim Yaser, Mohd Armi Abu Samah, Jualang Azlan Gansau, Syed Umar Faruq Syed Najmuddin
Summary: This study optimized the transesterification process to improve the efficiency of biodiesel production using WPCO. The results demonstrated that WPCO can be a potential feedstock for biodiesel production with the use of environmentally friendly biocatalysts like immobilized lipase.
Article
Chemistry, Multidisciplinary
Xiao-Meng Tang, Tian -Tian Ye, Xue-Jiao You, Xiao-Ming Yin, Jiang -Hui Ding, Wen-Xuan Shao, Meng -Yuan Chen, Bi-Feng Yuan, Yu-Qi Feng
Summary: In this study, a method for purifying plant ribosomal RNA (rRNA) was proposed and modifications in plant 18S rRNA and 25S rRNA were characterized using LC-ESI-MS/MS. The study revealed the presence of new modifications in plant rRNAs, including 2 modifications in 18S rRNA and 4 modifications in 25S rRNA. Exposure to cadmium induced significant changes in rRNA modifications, highlighting the importance of rRNA modifications in response to environmental stress. The discovery of new modifications expands our understanding of plant rRNA modifications and enhances the investigation of the functional roles of plant ribosomes in gene expression regulation.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Yan-Zhen Wang, Peng-Cheng Mei, Pei-Rong Bai, Na An, Jin-Gang He, Jie Wang, Quan-Fei Zhu, Yu-Qi Feng
Summary: Bile acids are important co-metabolites of gut microbiota and the host, maintaining metabolic homeostasis. A new mechanism of bile acid metabolism mediated by gut microbiota has been discovered, providing a new perspective for research on bile acid metabolism and gut metabolome.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Min Wang, Neng-Bin Xie, Ke-Ke Chen, Tong-Tong Ji, Jun Xiong, Xia Guo, Si-Yu Yu, Feng Tang, Conghua Xie, Yu-Qi Feng, Bi-Feng Yuan
Summary: DNA methylation (5mC) is a crucial epigenetic modification in mammals. The commonly used technique for quantitatively detecting 5mC, bisulfite sequencing (BS-seq), may cause DNA degradation. In this study, an engineered A3C sequencing (EAC-seq) method was proposed, which can map 5mC in DNA without bisulfite treatment and at single-base resolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sha Li, Zheng Zhang, Fei-Long Liu, Bi-Feng Yuan, Tian-Gang Liu, Yu-Qi Feng
Summary: Phosphomonoester metabolites play a crucial role in microbial biosynthesis and energy metabolism. Researchers have developed a LC-MS method using isotope labeling for the comprehensive analysis of phosphomonoester metabolites. They successfully identified 42 phosphomonoester metabolites, including two that were first detected in Saccharomyces cerevisiae. The relative quantification results showed significant differences in the content of these metabolites between lycopene-producible and wild-type S. cerevisiae, and further experiments confirmed the relationship between lycopene production and the activity of a specific pathway.
JOURNAL OF PROTEOME RESEARCH
(2023)
Review
Chemistry, Analytical
Jun Ding, Yu-Qi Feng
Summary: In clinical metabolomics, the use of mass spectrometry (MS) as an analytical platform is essential for characterizing metabolic disturbances, providing diagnostic evidence, and delivering qualitative and quantitative information of the broad metabolome in clinical samples. This review summarizes recent progress in sample collection, preparation, data acquisition, and normalization, and discusses the applications of metabolomics in biomarker screening, disease phenotyping, and clinical outcome prediction. The future directions of clinical metabolomics are also addressed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Cell Biology
Xiaolei Qiu, Zhuoyue Deng, Meijing Wang, Yuqi Feng, Lintao Bi, Lisha Li
Summary: Piezo ion channel, including Piezo1 and Piezo2, is a mechanosensitive protein on the cell membrane that responds to mechanical forces such as stretch, matrix stiffness, static pressure, and shear stress. These Piezo channels transmit mechanical signals and activate downstream pathways involved in stem cell differentiation, including integrin signaling, ERK1/2 MAPK signaling, Notch signaling, and WNT signaling. Differences in Piezo channel expression have been found in the fate of stem cell differentiation and are associated with developmental diseases. This review provides a comprehensive overview of the structure and function of Piezo channels, their relationship with mechanical signals, and their role in stem cell fate and developmental diseases.
Article
Nutrition & Dietetics
Hong-Li Gao, Xiao-Jing Yu, Yu-Qi Feng, Yu Yang, Han-Bo Hu, Yu-Yang Zhao, Jia-Hao Zhang, Kai-Li Liu, Yan Zhang, Li-Yan Fu, Ying Li, Jie Qi, Jin-An Qiao, Yu-Ming Kang
Summary: Luteolin, widely distributed in various plants, has diverse pharmacological effects including antioxidation, free radical scavenging, and anti-inflammation. This study aims to examine the effect of luteolin on the PI3K/Akt/NF-kappa B signaling pathway in the paraventricular nucleus (PVN) of hypertensive rats. The results showed that luteolin infusion significantly reduced hemodynamic parameters, expressions of oxidative stress and inflammatory markers, and increased the expression of antioxidant enzymes in the PVN. It revealed that luteolin can lower hypertension by inhibiting inflammation and PI3K/Akt signaling in the PVN.
Article
Biochemistry & Molecular Biology
Maria Zorina, Victor V. Dotsenko, Pavel N. Nesterenko, Azamat Temerdashev, Ekaterina Dmitrieva, Yu-Qi Feng, Sanka N. Atapattu
Summary: In this study, phthalylglycyl chloride was proposed as a derivatization agent for the determination of octopamine, adrenaline, and dopamine in urine for the first time. The derivatization process combined reversed-phase liquid chromatography with positive electrospray ionization-high-resolution mass spectrometry. An LC-HRMS method with quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes was developed. The derivatization with phthalylglycyl chloride at pH 6.5 and 30 degrees C resulted in a 95-97% yield of derivatives. The proposed method was successfully applied to the analysis of human urine samples and compared with conventional derivatization procedures using 9-fluorenyl-methoxycarbonyl chloride and dansyl chloride.
Article
Chemistry, Multidisciplinary
Cheng-Jie Ma, Gaojie Li, Wen-Xuan Shao, Yi-Hao Min, Ping Wang, Jiang-Hui Ding, Neng-Bin Xie, Min Wang, Feng Tang, Yu-Qi Feng, Weimin Ci, Yinsheng Wang, Bi-Feng Yuan
Summary: Researchers developed a method called deaminase-mediated sequencing (DM-seq) to map the presence of 6mA in DNA at single-nucleotide resolution. They found that 6mA is mainly located in the GATC motif in E. coli genome and identified 17 6mA sites in mtDNA of HepG2 cells, all of which are in the heavy strand. DM-seq is expected to be a valuable tool for studying the functions of 6mA in DNA and its potential roles in mitochondria-related human diseases.
ACS CENTRAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Linlin Qin, Na An, Bifeng Yuan, Quanfei Zhu, Yuqi Feng
Summary: Lipid reprogramming metabolism plays a crucial role in breast cancer growth and potential biomarkers. Fatty acid esters of hydroxy fatty acids (FAHFAs), a class of endogenous lipid metabolites, have been found to have anti-diabetic and anti-inflammatory properties. In this study, the researchers aimed to explore the relationship between FAHFAs and breast cancer by analyzing FAHFAs in tumors and adjacent normal tissues using chemical isotope labeling combined with liquid chromatography-mass spectrometry (CIL-LC-MS). Statistical analysis identified 13 altered isomers in breast cancer, which showed potential in distinguishing breast cancer tissues. Furthermore, the up-regulation of 9-oleic acid ester of hydroxy stearic acid (9-OAHSA) and down-regulation of 9-hydroxystearic acid (9-HSA) in tumors suggest that breast cancer shares similarities with colorectal cancer in terms of attenuating pro-apoptotic effects and promoting tumor survival and progression through FAHFA synthesis.
Article
Chemistry, Analytical
Yaqi Zhu, Saiyi Zhong, Xitian Peng, Qiongwei Yu, Yuqi Feng
Summary: A simple and sensitive method combining solid-phase extraction (SPE) and high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was developed for the determination of benzo[a]pyrene (BaP) in fish. Loofah sponge (LS) was carbonized and used as an SPE adsorbent. The optimized method showed good accuracy and precision with a wide linear range and low detection limit, making it a low-cost and environmentally friendly alternative for the determination of BaP in aquatic products.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Min Wang, Neng-Bin Xie, Ke-Ke Chen, Tong-Tong Ji, Jun Xiong, Xia Guo, Si-Yu Yu, Feng Tang, Conghua Xie, Yu-Qi Feng, Bi-Feng Yuan
Summary: DNA methylation (5mC) is an important epigenetic modification in mammals and its roles are deciphered by detecting 5mC at single-base resolution. Bisulfite sequencing (BS-seq) is commonly used for mapping 5mC but causes DNA damage. Here, we engineered the A3C protein to develop an engineered A3C sequencing (EAC-seq) method, which can quantitatively map 5mC without bisulfite treatment. The EAC-seq approach, employing mild reaction conditions, is a valuable tool for the quantitative detection of 5mC at single-base resolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sha Li, Zheng Zhang, Fei-Long Liu, Bi-Feng Yuan, Tian-Gang Liu, Yu-Qi Feng
Summary: We developed an LC-MS method using isotope reagents for the comprehensive analysis of phosphomonoester metabolites. This method allowed for rapid screening, identification, and relative quantification of the metabolites in Saccharomyces cerevisiae. We identified 42 phosphomonoester metabolites, including two that were detected for the first time in S. cerevisiae. The relative quantification results revealed significant differences in certain metabolites between lycopene-producible and wild-type S. cerevisiae, indicating a close relationship between phosphomonoesters and lycopene production.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Multidisciplinary Sciences
Na An, Yu Wang, Dong-Xiao He, Peng-Cheng Mei, Quan-Fei Zhu, Yu-Qi Feng
Summary: This study analyzed the presence and variety of FAHFAs in different foods using chemical isotope labeling-assisted liquid chromatography-mass spectrometry. The findings revealed the molecular diversity of these lipids and their presence in various foods, providing a foundation for further exploration of the nutritional and medicinal functions of FAHFAs.
Article
Chemistry, Analytical
Qin-Feng Zhang, Hua-Ming Xiao, Na An, Quan-Fei Zhu, Yu-Qi Feng
Summary: A chemical derivatization assisted LC-MS/MS method was developed for the detection and quantification of vitamin D metabolites. The method significantly increased the sensitivities of the metabolites and allowed for accurate quantification in various biological samples. This method provides a promising tool for studying vitamin D metabolism.
ANALYTICAL METHODS
(2023)
