Article
Biochemical Research Methods
Matthias Hermann, Prashant Agrawal, Chang Liu, J. C. Yves LeBlanc, Thomas R. Covey, Richard D. Oleschuk
Summary: The rapid calibration chip (RCC) uses the wetting behavior of hydrophilic/hydrophobic patterns to confine droplets and obtain a calibration curve. It doesn't require advanced equipment and can form multiple droplet series quickly. Evaluation of its performance allows for determining liquid volumes.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Luan Felipe Campos Oliveira, David Ulisses Tega, Marcos Nogueira Eberlin, Alessandra Sussulini
Summary: V-EASI is a soft ambient ionization source suitable for samples in solution, without the need for voltage or heat, with low sample preparation requirements. The study validated the quantification method for bixin in annatto using the LC-V-EASI-MS system, showing good performance.
Article
Thermodynamics
Abdullah Al-Sharafi, Bekir S. Yilbas, Ahmet Z. Sahin, H. Al-Qahtani
Summary: During the heating and stretching of the water droplet, necking of the droplet in the top plate region and elongated circulating structures in the vertical direction are observed. Additionally, the Nusselt number reaches large values prior to droplet stretching but decreases after stretching.
INTERNATIONAL COMMUNICATIONS IN HEAT AND MASS TRANSFER
(2021)
Article
Materials Science, Coatings & Films
Cun Lv, Faqiang Wang, Caixia He, Jialiang Kang, Xiaoxiang He, Zhanxiong Li
Summary: Microspheres with a rough structure were prepared using blends of poly(epsilon-caprolactone) and ladder-like fluorinated polysilsesquioxane via electrostatic spraying, demonstrating excellent superhydrophobicity with optimized spraying and blending ratio. The finished cotton fabric coated with the microspheres showed excellent hydrophobicity and self-cleaning function, with good mechanical stability after sandpaper abrasion and adhesion tests.
SURFACE & COATINGS TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Yuze Li, Junyu Chen, Lingwei Meng, Liuying He, Huihui Liu, Caiqiao Xiong, Zongxiu Nie
Summary: The MasSpec Pointer is a wireless pocket-size device powered by a 3.7 V polymer Li battery, which can be used for ambient ionization mass spectrometry. It has stable discharge and good quantification performance, allowing for quick testing of various samples in both positive and negative ion modes. The device can detect pesticides, chemicals, and other substances without any modification, and has a detection limit lower than 0.1 ng/mL for atrazine.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Ceramics
Ali Ansari, Nowrouz Mohammad Nouri
Summary: A repeatable and environmentally friendly method was used to prepare a hydrophobic surface with photocatalytic features. Titanium dioxide nanoparticles and fluorinated ethylene propylene (FEP) resins were applied on an aluminum substrate using layer-by-layer and mixing methods. The prepared films were analyzed and the photocatalytic activities were explored. The hydrophobicity and photocatalytic performance were better in the samples with one layer of titanium dioxide and three layers of FEP in the layer-by-layer method, and in the sample containing 10 wt% TiO2 in the mixing method.
CERAMICS INTERNATIONAL
(2023)
Article
Chemistry, Applied
Jialiang Kang, Xueting Wang, Xiaoxiang He, Cun Lv, Caixia He, Zhanxiong Li
Summary: The hydrophobic block copolymer PCL-b-PFPE was successfully synthesised by reacting PFPE-COOH with PCL. The surface morphology of the microspheres was influenced by the solvent and ambient humidity, with chloroform forming smooth microspheres and DMF/CF mixed solvent producing a 'pit' surface morphology. The introduction of PFPE chain into PCL enhanced the hydrophobicity of coatings, providing super-hydrophobicity to the finished polyester fabric.
PROGRESS IN ORGANIC COATINGS
(2023)
Article
Chemistry, Analytical
Vincen Wu, Jocelyn Tillner, Emrys Jones, James S. McKenzie, Dipa Gurung, Anna Mroz, Liam Poynter, Daniel Simon, Cristina Grau, Xavier Altafaj, Marc-Emmanuel Dumas, Ian Gilmore, Josephine Bunch, Zoltan Takats
Summary: This study examines the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue, comparing its performance to the traditional desorption electrospray ionization (DESI). The flow focusing mechanism in DESI offers advantages such as a rotationally symmetric electrospray jet, higher intensity, controllable parameters, and better portability. The optimized parameters for DEFFI result in similar images to DESI but with better sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Kaixing Luo, Yu Luo, Yanjuan Liu, Yuefei Zhang, Wei Chen, Zhengwu Bai, Sheng Tang
Summary: In this work, a new strategy was adopted to simultaneously graft two different functional ligands onto the silica surface, resulting in a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) using recycled commercial C18 columns as raw materials. The obtained Sil-CDI-DD exhibited enhanced separation selectivity and efficiency, providing multiple interaction sites for diverse target analytes and enabling the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Physical
Di Zhou, Ping Fu, Tian Gao, Zhi-Kang Xu, Ling-Shu Wan
Summary: In this work, fluorinated polymers with perfluoroalkyl groups and hydrophilic oligo(ethylene glycol) units were designed and synthesized. The elongation of hydrophilic segments and functionalization with perfluoroalkyl end groups decreased the interfacial tension and ability of the polymers to precipitate at the interface. This study suggests that low interfacial tension and strong capability of interfacial precipitation are beneficial to droplet stabilization and honeycomb pattern formation.
Article
Engineering, Biomedical
Yahya Efe Yayoglu, Ryan G. Toomey, Nathan B. Crane, Nathan D. Gallant
Summary: Magnesium and its alloys are considered promising materials for medical implants due to their biocompatibility and mechanical properties. However, the degradation rate of magnesium in aqueous environments is too high for most applications. A study found that hydrophobic textured surfaces can reduce corrosion rates on magnesium, but the specific role of texture and hydrophobic treatments in corrosion rates needs further investigation.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Engineering, Electrical & Electronic
Anwaruddin Siddiqui Mohammed, Bekir Sami Yilbas, Hussain Al-Qahtani, Abba Abdulhamid Abubakar, Muhammed Hawwa, Mahmood Kassas
Summary: This study examines the dynamics of dust mitigation from inclined hydrophilic/hydrophobic glass surfaces after introducing electrostatic repulsion. The findings suggest that hydrophobizing the surfaces reduces particle adhesion and the force of adhesion remains larger for smaller particles. Surface tilting influences the dynamics of inflight particles, dust mitigation rate, and optical characteristics of the surfaces.
JOURNAL OF ELECTROSTATICS
(2021)
Article
Polymer Science
Betul Ari, Sahin Demirci, Ramesh S. Ayyala, Bekir Salih, Nurettin Sahiner
Summary: One-step, simple preparation of superporous p(beta-cyclodextrin) (p(beta-CD)) cryogels under cryogenic conditions was reported for the first time. The prepared cryogels exhibited excellent swelling properties and showed potential as drug delivery systems for both hydrophilic and hydrophobic drugs.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Environmental Sciences
Hilary M. Brown, Patrick W. Fedick
Summary: 3D-PCSI-MS is a rapid method for detecting trace PFAS in solid media through ambient ionization mass spectrometry, with advantages of low cost, scalability, and high-throughput screening of bulk samples without sample preparation.
Article
Chemistry, Analytical
Rui Guo, Qisheng Zhong, Jiaqi Liu, Peiming Bai, Zongpeng Wang, Jieling Kou, Peijie Chen, Jie Zhang, Bo Zhang
Summary: In this study, a method called 2D-LC/TQMS was developed for simultaneous analysis of hydrophilic and hydrophobic metabolites using the combination of HILIC and RPLC. The method can detect 417 metabolites involved in various metabolic pathways with good reproducibility, linearity, and wide linear range. It was tested using different biological samples and applied to a bladder cancer case/control study, identifying 30 differential metabolites related to carbohydrate and amino acid metabolism.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Ying He, Shurong Wang, Xinyue Han, Jiayuan Shen, Yanwei Lu, Jinzhi Zhao, Chengpin Shen, Liang Qiao
Summary: This study reports an artificial photosynthesis system driven by light without the need for additional power sources. By using Sporomusa ovata-CdS biohybrids, the conversion of light energy to electric energy for carbon dioxide fixation was achieved, with a high quantum yield and long active duration time. Proteomic analysis revealed up-regulation of key enzymes in the biohybrid system, providing insights into the mechanism of artificial photosynthesis and guiding the development of new biohybrid systems using genetically engineered microbes in the future.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Guobin Han, Dandan Li, Qiuyuan Lin, Jia Yi, Qian Lyu, Qingwei Ma, Liang Qiao
Summary: In this work, a method combining EXPAR and MALDI-TOF MS is developed for label-free and simultaneous detection of multiple low-abundance miRNAs. The method can be performed in a single reaction tube under isothermal conditions and completed in less than 30 minutes. It demonstrates good quantification ability, attomolar-level sensitivity, and high specificity for miRNAs detection, including single-nucleotide resolution. Therefore, this label-free mass-spectrometric platform has the potential to be a valuable tool for miRNA analysis in complex biological samples and clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Guobin Han, Qiuyuan Lin, Jia Yi, Qian Lyu, Qingwei Ma, Liang Qiao
Summary: The researchers developed a new method for the rapid and accurate detection of SARS-CoV-2 using a three-way junction induced exponential rolling circle amplification (3WJ-eRCA) combined with MALDI-TOF MS assay. The method showed high specificity in distinguishing SARS-CoV-2 from other coronaviruses and demonstrated potential for large-scale screening of COVID-19.
Review
Biochemical Research Methods
Yi Yang, Liang Qiao
Summary: Protein post-translational modifications (PTMs) increase the functional diversity of the cellular proteome. Accurate quantification and identification of protein PTMs is crucial in proteomics research. Recent advancements in data-independent acquisition (DIA) mass spectrometry (MS) have enabled deep coverage and accurate quantification of PTMs. This review provides an overview of DIA data processing methods for PTMs analysis, as well as deep learning methods that enhance PTMs analysis. The limitations and future directions of DIA methods for PTMs analysis are also discussed.
Article
Physics, Atomic, Molecular & Chemical
Liang Qiao
Summary: Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is widely used for bacterial identification in clinical laboratories. However, the current technique requires bacterial culture for MS analysis, limiting its application in direct bacterial antimicrobial resistance (AMR) analysis. Researchers have focused on improving bacterial identification and AMR analysis using MALDI-TOF MS, developing new methods for sample pretreatment, data acquisition, and data mining to achieve more accurate and rapid bacterial identification and direct AMR analysis. Microfluidic chips and functional nanomaterials can extract bacterial cells directly from raw materials, shortening or eliminating the need for bacterial culture. Single cell mass spectrometry techniques and advanced data mining strategies based on machine learning and deep learning techniques are expected to play important roles in bacterial identification and AMR analysis. With the development of sample pretreatment methods, MS techniques, and data analysis algorithms, the MALDI-TOF MS technique has the potential to significantly advance the clinical treatment of bacterial infectious diseases.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2022)
Article
Engineering, Biomedical
Xinyue Han, Dandan Li, Shurong Wang, Yuxiang Lin, Yun Liu, Ling Lin, Liang Qiao
Summary: In this study, a non-invasive liquid biopsy method was developed for the diagnosis of TNBC by quantifying free serum amino acids using colloidosomes-coupled MALDI-TOF MS. A machine learning-based classification model was built based on the quantities of serum free amino acids to differentiate TNBC patients from the controls. The method showed high sensitivity, specificity, and accuracy. With the advantages of low cost, low sample consumption, high throughput in analysis, and high accuracy in identification, this method is promising for clinical diagnosis of TNBC.
MATERIALS TODAY BIO
(2022)
Review
Biochemical Research Methods
Yanjun Liu, Ling Lin, Liang Qiao
Summary: Cardiovascular diseases are a major cause of illness and death worldwide, and current research relies on animal and cell culture models. However, these models have limitations in faithfully replicating human response and the in vivo microenvironment. Organ-on-a-chip technology, which combines microfabrication and tissue engineering, offers a promising solution. This review discusses the methods and materials used to create organ-on-a-chip systems, with a specific focus on the construction of vessel and heart chips. These systems can guide future cardiovascular disease research by incorporating mechanical and fluid forces and enabling the study of cardiomyocyte maturation.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Zengyu Wang, Baohong Liu, Ling Lin, Liang Qiao
Summary: Mitochondria are highly dynamic organelles involved in various cellular processes. Dysfunction of mitochondria is associated with multiple disorders, including neurodegenerative diseases, cardiovascular pathology, aging, and cancer. Significant advancements have been made in mitochondrial biology through the combination of high-throughput omics technologies and biochemical separation techniques. This review focuses on mass spectrometry-based mitochondrial proteomics, metabolomics, and multi-omics integration, as well as the development of machine learning tools for comprehensive and unprecedented insight into mitochondrial uncharted territory.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Yu Zong, Yuxin Wang, Yi Yang, Dan Zhao, Xiaoqing Wang, Chengpin Shen, Liang Qiao
Summary: DeepFLR is a deep learning-based framework that accurately predicts phosphopeptide tandem mass spectra and effectively controls false localization rates in phosphoproteomics.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Dongxue Zhang, Fan Yin, Qin Qin, Liang Qiao
Summary: Bacterial antimicrobial resistance (AMR) is a major challenge to current society, and understanding the molecular changes during bacterial self-saving responses can lead to new inhibition methods. This study used microfluidics mass spectrometry to identify metabolic changes in extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E. coli) during filamentation. The up-regulation of cyclic dinucleotide c-di-GMP, which is related to bacterial resistance and tolerance, was observed. By inhibiting the synthesis of c-di-GMP through a chemical inhibitor, the minimum inhibitory concentration of ceftriaxone against ESBL-E. coli was significantly decreased. This study uncovers molecular changes during bacterial filamentation and proposes a method to inhibit antibiotic-resistant bacteria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Chemistry, Physical
Dongxue Zhang, Liang Qiao
Summary: Population-level analysis masks heterogeneity between individual cells. Microfluidics coupled with mass spectrometry is a promising technique for studying single-cell omics. This review summarizes microfluidic chips designed for single-cell manipulation and discusses their applications in single-cell proteomics, metabolomics, lipidomics, and multi-omics.
Review
Nanoscience & Nanotechnology
Dongxue Zhang, Liang Qiao
Summary: Intestinal diseases have a significant impact on quality of life. Traditional methods of studying intestinal diseases using cell culture and animal models are not accurate representations of human physiology and pathophysiology. Intestine-on-a-chip, which mimics the human intestinal microenvironment, is a promising tool for studying intestinal diseases. This review summarizes the key elements of reconstructing the intestinal microenvironment, the different models of intestine-on-a-chip, and the commonly used analytical techniques for studying the intestinal microenvironment.
Article
Chemistry, Physical
Wanderson O. Silva, Bhawna Nagar, Dennis Ellersiek, Luc Bondaz, Jordi Espin, Mathieu Soutrenon, Hubert H. Girault
Summary: Waste tires are a major environmental concern due to their non-degradable nature and the large area occupied by them in landfills worldwide. Pyrolysis, especially photo-pyrolysis, has gained attention as a recycling process that can minimize toxic gas emissions. This study presents a new and eco-friendly strategy for treating waste tires, using high-intensity flash light irradiation to convert them into valuable products such as tire pyrolysis oil (TPO), syngas, and a carbon-rich solid residue.
SUSTAINABLE ENERGY & FUELS
(2023)
Article
Chemistry, Analytical
Yi-Hong Lin, Chih-Ning Tsai, Po-Feng Chen, Yen-Tzu Lin, Sorour Darvishi, Hubert H. Girault, Tung-Yi Lin, Mei-Yi Liao, Tzu-En Lin
Summary: Scanning electrochemical microscopy (SECM) is a scanning probe technique that allows for the study of surface morphology and electrochemical reactivity. The quality of SECM images is affected by various factors, including electrode size and other uncontrollable factors. To overcome these challenges, researchers have developed soft gold probes and utilized AI-assisted image fusion to improve the quality of SECM images.
ACS MEASUREMENT SCIENCE AU
(2022)
Article
Chemistry, Multidisciplinary
Sara N. Moya Betancourt, Julieta S. Riva, Jorge G. Uranga, Astrid J. Olaya, Hubert H. Girault
Summary: The study investigates the visible light driven water oxidation reaction using the organic electron acceptor TCNQF(4) without any metal or organometallic catalysts. The generated oxygen and protons were measured to monitor the reaction efficiency. This work opens new perspectives for water photo-oxidation in liquids and artificial photosynthesis.
CHEMICAL COMMUNICATIONS
(2022)
