Journal
RSC ADVANCES
Volume 5, Issue 76, Pages 62300-62305Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra13279g
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Funding
- Research Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-ZZB-201307]
- earmarked fund for Jiangxi Agriculture Research System [JXARS-03]
- Nanchang Technological Program [2012-CYH-DW-SP-001]
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A colloidal gold immunochromatographic signal-amplifying test strip with a sandwich format was successfully developed to detect Escherichia coli O157:H7. The detection signal was improved by addition of enhancers (HAuCl4 and NH2OH center dot HCl) to the nitrocellulose membrane of a conventional immunochromatographic strip. The detection sensitivities of the test strip before and after signal amplification were 4 x 10(4) and 5 x 10(3) CFU mL(-1), respectively, as determined by a colloidal gold strip reader. An 8-fold increase in sensitivity after signal amplification was accomplished within 20 min. No cross-reactivity with 27 non-target strains was observed, and the time for detecting E. coli O157:H7 in spiked milk samples was shortened by 1.5 h. The colloidal gold immunochromatographic signal-amplifying test strip thus provides a novel method for determining E. coli O157:H7 and is rapid, sensitive, convenient, and suitable for on-site detection.
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