Article
Biochemistry & Molecular Biology
Sayandeep Gupta, Mousam Roy, Dhritiman Dey, Koustav Bhakta, Arghya Bhowmick, Dhrubajyoti Chattopadhyay, Abhrajyoti Ghosh
Summary: The study demonstrates the important role of archaeal 7S RNA in stabilizing the SRP54$FtsY targeting complex, with further assistance from SRP19. These results suggest that archaeal 7S RNA and SRP19 reinforce the targeting complex to execute protein translocation through modulation of its conformation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zachary R. Grese, Alliny C. S. Bastos, Lohany D. Mamede, Rachel L. French, Timothy M. Miller, Yuna M. Ayala
Summary: TDP-43 is an RNA-binding protein that forms ribonucleoprotein condensates through liquid-liquid phase separation, playing a central role in regulating gene expression and protein homeostasis. RNA binding promotes TDP-43 condensation through specific interactions, maintaining the liquid-like properties of condensates. Defects in this RNA-mediated activity may contribute to TDP-43-associated pathogenesis, particularly in neurodegenerative disorders such as ALS and frontotemporal dementia.
Article
Biochemistry & Molecular Biology
Priyanka Choudhury, Jens Kretschmer, Philipp Hackert, Katherine E. Bohnsack, Markus T. Bohnsack
Summary: RNA helicase DDX55 is predominantly localized in the nucleoplasm in human cells and associates with pre-ribosomal subunits, playing a crucial role in the maturation of large subunit pre-rRNAs. The C-terminal region of DDX55 is essential for its substrate specificity, recruitment to pre-ribosomes, and may target domain IV during LSU biogenesis.
Article
Biochemistry & Molecular Biology
Sandra M. Fernandez-Moya, Janina Ehses, Karl E. Bauer, Rico Schieweck, Anob M. Chakrabarti, Flora C. Y. Lee, Christin Illig, Nicholas M. Luscombe, Max Harner, Jernej Ule, Michael A. Kiebler
Summary: RNA secondary structures play a crucial role in regulating the assembly, localization, and translation of RNA granules, with different effects in neurons. Disrupting RNA hairpins impairs the observed effects of RNA localization and assembly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Hui Li, Hailian Zhao, Chunhui Yang, Ruibao Su, Min Long, Jinliang Liu, Lanying Shi, Yuanchao Xue, You-Qiang Su
Summary: Mammalian oogenesis relies on stored mRNAs for successful development, but how their fate is determined remains unclear. RNA-binding proteins (RBPs) are important regulators, but the full complement in oocytes is unknown. This study identified 1396 RBPs in mouse oocytes and found LSM14B to be an important RBP specific to oocytes, essential for oogenesis and proper translation of its bound mRNAs.
Article
Chemistry, Analytical
Dong-Hwa Lee, Sohee Oh, Kyungeun Lim, Boah Lee, Gwan-Su Yi, Young-Rok Kim, Ki-Bum Kim, Chong-Kil Lee, Seung-Wook Chi, Mi-Kyung Lee
Summary: Bacterial riboswitch RNAs are potential targets for novel antibiotics against antibiotic-resistant superbacteria. This study demonstrated the specific interaction between the adenine-sensing riboswitch aptamer domain and adenine at the single-molecule level using alpha-hemolysin nanopores, which could be a valuable platform for ultrasensitive drug screening against therapeutic RNA targets.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Haina Huang, Katrin Karbstein
Summary: This passage discusses the tendency of three-helix junctions to misfold during the assembly of small ribosomal subunits in cells, and reveals the pivotal role of ribosome assembly factors in facilitating their correct folding.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Steve L. Bonilla, Sarah K. Denny, John H. Shin, Aurora Alvarez-Buylla, William J. Greenleaf, Daniel Herschlag
Summary: Despite RNA's diverse structures, nature uses a limited set of structural motifs to build functional RNAs. Motifs can be classified into subclasses based on sequence and structure, but little is known about these subclasses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Tom Dendooven, Elisabeth Sonnleitner, Udo Blaesi, Ben F. Luisi
Summary: The bacterial RNA chaperone Hfq and its partner protein Crc form higher order assemblies on control regions of different P. aeruginosa target mRNAs. These assemblies have mRNA-specific quaternary architectures due to the combination of multivalent protein-protein interfaces and recognition of patterns in the RNA sequence. The structural polymorphism of these ribonucleoprotein assemblies enables selective translational repression of many different target mRNAs.
Article
Biochemical Research Methods
Corinna J. Ulshoefer, Christina Pfafenrot, Albrecht Bindereif, Tim Schneider
Summary: CircRNAs have been extensively studied in recent years, revealing functional roles in various cell types and organisms. However, the specific interaction partners for most circRNAs remain unknown due to technical difficulties, presenting challenges in systematically studying circRNA-protein complexes.
Article
Biochemistry & Molecular Biology
Takahiro Sawada, Koichi Nishimura, Jinichi Mori, Yoshiaki Kanemoto, Alexander Kouzmenko, Rei Amano, Akira Hayakawa, Suguru Tokiwa, Hiroaki Shimmura, Shigeaki Kato
Summary: This study identified a molecular basis for androgen-induced association of AR with chromatin on ARE-unrelated sequences, highlighting the important role of enhancer RNAs in this process.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Julianna Han, Ketaki Ganti, Veeresh Kumar Sali, Carly Twigg, Yifeng Zhang, Senthamizharasi Manivasagam, Chieh-Yu Liang, Olivia A. Vogel, Iris Huang, Shanan N. Emmanuel, Jesse Plung, Lillianna Radoshevich, Jasmine T. Perez, Anice C. Lowen, Balaji Manicassamy
Summary: It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion, but the exact role of host factors in genome assembly is not fully understood. This study shows that Rab11a+ vesicles play a crucial role in the congregation and assembly of vRNPs, enabling specific genome assembly and production of infectious virus particles. However, in the absence of Rab11a, viral RNA segments fail to congregate properly, resulting in defects in virus assembly and increased production of non-infectious particles.
Article
Biochemistry & Molecular Biology
Tyler Matheny, Briana Van Treeck, Thao Ngoc Huynh, Roy Parker
Summary: The study reveals that specific RNA and protein can influence the enrichment of RNA in stress granules. Artificial tethering of certain proteins can target mRNA into stress granules with multiple interactions required for efficient partitioning. This suggests a summation of RNA-protein, protein-protein, and RNA-RNA interactions collectively determine RNA recruitment into stress granules.
Article
Biochemistry & Molecular Biology
Margaret L. Rodgers, Yunsheng Sun, Sarah A. Woodson
Summary: During transcription, ribosomal subunits begin assembly by folding rRNA and associating it with ribosomal proteins (RPs). The initial recognition of properly folded rRNA by primary assembly proteins, like S4, is delayed due to the variable folding of rRNA during transcription. Late-binding RP S12 promotes the association of S4 with pre-16S rRNA, accelerating ribosome assembly.
Article
Biochemistry & Molecular Biology
Lisa N. Hansen, Otto A. Kletzien, Marcus Urquijo, Logan T. Schwanz, Robert T. Batey
Summary: In this study, the authors investigate three different T-loop receptor motifs found in FMN and Cbl riboswitches using a cell-based genetic screen and bioinformatic analysis. They find clear sequence signatures for these receptor motifs that align with phylogenic analysis and identify functional differences in TL/TLR interactions within the riboswitch subclass.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
