Review
Clinical Neurology
Sarah Nilkece Mesquita Araujo Nogueira Bastos, Barbara Louise Freire Barbosa, Sangela Fernandes Silva, Ana Gabriela Krymchantowski, Carla Jevoux, Abouch Krymchantowski, Raimundo Pereira Silva-Neto
Summary: This study analyzed 51 cases of cluster headache in pediatric patients published between 1990 and 2020, finding that the pain typically occurs at night or upon waking, with short duration, unilateral location, accompanying autonomic symptoms, and effective treatments including sumatriptan and oxygen inhalation.
DEVELOPMENTAL MEDICINE AND CHILD NEUROLOGY
(2021)
Article
Nursing
Fernanda Machado Silva-Rodrigues, Milena de Lucca, Ana Carolina Andrade Biaggi Leite, Willyane de Andrade Alvarenga, Michele Darezzo Rodrigues Nunes, Lucila Castanheira Nascimento
Summary: The study describes the strategies used by family caregivers of pediatric cancer patients in managing chemotherapy-associated symptoms. Various types of symptoms were mentioned, including physical, emotional, and constitutional symptoms, with pharmacological and non-pharmacological measures being used for their management.
REVISTA DA ESCOLA DE ENFERMAGEM DA USP
(2021)
Article
Nursing
Divane de Vargas, Enka Gisseth Leon Ramirez, Caroline Figueira Pereira, Sheila Ramos de Oliveira
Summary: The objective of this study was to investigate the effect of remote intervention on anxiety symptoms and alcohol use in users of primary health care service. A quasi-experimental study was conducted with 1270 participants who completed the Alcohol Use Disorders Identification Test and the State-Trait Anxiety Inventory 6. Of these, 1033 participants scored for moderate/severe anxiety symptoms (STAI-6>3) and moderate/severe alcohol use risk (AUDIT-C>3), and received interventions via phone calls with seven-day and 180-day follow-ups. Mixed-effects regression model was used for data analysis. The results showed that the intervention had a positive effect on reducing anxiety symptoms between T0 and T1 (mu=1.6, p<0.001) and reducing alcohol use pattern between t1 and t3 (mu=1.57, p<0.001). In conclusion, the follow-up results suggest a positive effect of the intervention in reducing anxiety and alcohol use pattern, which tends to be maintained over time. There is evidence that the proposed intervention can be an alternative for preventive mental health care in situations where user or professional accessibility is compromised.
REVISTA LATINO-AMERICANA DE ENFERMAGEM
(2023)
Article
Environmental Sciences
Paulo Renato Souza de Oliveira, Paulo Fernando Trugilho, Tiago Jose Pires de Oliveira
Summary: The study aims to characterize the biomass of acai seeds and establish technical parameters for producing high-quality briquettes, with temperature having a greater influence on the characteristics of the briquettes than pressure. Higher temperature and lower pressure resulted in better compression strength for the briquettes, while increased pressure led to decreased water absorption rate. Overall, improving pressure and utilizing temperature effectively can enhance the quality and energy density of the briquettes, making them suitable for combustion in boilers.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Miao Gui, Hannah Farley, Priyanka Anujan, Jacob R. Anderson, Dale W. Maxwell, Jonathan B. Whitchurch, J. Josephine Botsch, Tao Qiu, Shimi Meleppattu, Sandeep K. Singh, Qi Zhang, James Thompson, Jane S. Lucas, Colin D. Bingle, Dominic P. Norris, Sudipto Roy, Alan Brown
Summary: The study provides a detailed analysis of the 48-nm repeat model of mammalian doublet microtubules, uncovering specific features and previously unknown MIPs specific to vertebrate cilia. Loss of proteins involved leads to defective ciliary motility and laterality abnormalities in zebrafish and mice. This research helps identify candidate genes for ciliopathies and understand their functions in driving ciliary motility.
Article
Pediatrics
Patricia Marques, Tiago Madeira, Augusta Gama
Summary: This study investigates the characteristics of the menstrual cycle and the prevalence of dysmenorrhea in students from the Lisbon region. The results suggest that menstrual disorders are common and may be associated with early menarche, but not with BMI. Encouraging self-monitoring of the menstrual cycle is important for timely detection of menstrual disorders and promoting health and well-being.
REVISTA PAULISTA DE PEDIATRIA
(2022)
Article
Biochemistry & Molecular Biology
Dong Si, Jason Chen, Andrew Nakamura, Luca Chang, Haowen Guan
Summary: The study of macromolecular structures has revolutionized the pharmaceutical industry's development of new vaccines. While X-ray crystallography has been the traditional method, cryo-EM is gaining popularity due to recent advancements. Automated software is desired for labor-intensive de novo macromolecular complex modeling. This paper presents deep learning-based methods for macromolecule modeling applications.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Food Science & Technology
Sara Kosenina, Markel Martinez-Carranza, Jonathan R. Davies, Geoffrey Masuyer, Pal Stenmark
Summary: Botulinum neurotoxins (BoNTs) are highly toxic causative agents of paralytic diseases that have been widely used in cosmetic and therapeutic applications. This study utilized single-particle cryogenic electron microscopy to determine the structures of BoNT/B and /E, highlighting the structural variations and dynamics of these toxins. This research demonstrates the potential of electron microscopy in studying bacterial toxins, particularly the botulinum neurotoxin family.
Review
Neurosciences
Ronney Pinto Lopes, Vivian Dias Baptista Gagliardi, Felipe Torres Pacheco, Rubens Jose Gagliardi
Summary: This article reviewed the clinical and radiological profile of patients with unknown-onset stroke, the imaging methods for guiding reperfusion treatment, and proposed a treatment protocol. Studies found no significant differences between the clinical and imaging characteristics of patients with unknown-onset stroke and those with known-onset stroke. The length of the therapeutic window is based on the difference between the diffusion penumbra and clinical core mismatch. Challenges in approaching unknown-onset stroke include extending the length of the treatment window, reproducibility of real-world imaging modalities, and discovering new methods.
ARQUIVOS DE NEURO-PSIQUIATRIA
(2022)
Article
Biophysics
Luca Chang, Fengbin Wang, Kiernan Connolly, Hanze Meng, Zhangli Su, Virginija Cvirkaite-Krupovic, Mart Krupovic, Edward H. Egelman, Dong Si
Summary: The recent revolution in cryo-electron microscopy has allowed for the direct determination of macromolecular structures from cell extracts. However, accurately identifying the correct protein from a cryo-EM map is still challenging and often requires additional information. In this study, the researchers propose a server-based method called DeepTracer-ID that can identify candidate proteins from a cryo-EM map without the need for extra information. The method utilizes DeepTracer to generate a protein backbone model and searches it against a library of AlphaFold2 predictions for all proteins in the organism. The method showed high accuracy and robustness in identifying proteins from high-resolution cryo-EM maps.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Shingo Nojima, Yoko Fujita, Kanako Terakado Kimura, Norimichi Nomura, Ryoji Suno, Kazushi Morimoto, Masaki Yamamoto, Takeshi Noda, So Iwata, Hideki Shigematsu, Takuya Kobayashi
Summary: EP4, a class A GPCR, is a common drug target in various disorders. The structure features of EP4-Gs binding are formed by specific conserved residues, which may be important for understanding the binding mechanism of G protein.
Article
Forestry
Thairini Claudino Zavistanovicz, Suelen Carpenedo Aimi, Maristela Machado Araujo, Alvaro Luis Pasquetti Berghetti, Daniele Rodrigues Gomes, Felipe Manzoni Barbosa
Summary: The study showed that using plastic bags and mulching can promote the growth of Casearia sylvestris, Handroanthus heptaphyllus, and Parapiptadenia rigida seedlings in altered areas. However, there was no significant impact on physiological parameters among different treatments.
Article
Nursing
Tugba Sahbaz, Cansin Medin-Ceylan
Summary: The objective of this study is to examine the relationship between musculoskeletal problems experienced by family caregivers of stroke patients, physical health, and patient's level of disability. The results showed that low back pain was associated with Functional Ambulation Score, Functional Independence Measure, Stroke Impact Scale, and patient's Brunnstrom test. Neck pain was the second musculoskeletal complaint but not statistically associated with patient-related factors. Upper extremity problems were associated with scores on several scales. The study highlights the impact of musculoskeletal complaints on family caregivers of stroke patients and their relationship with patient's functional capacity and level of disability.
REVISTA LATINO-AMERICANA DE ENFERMAGEM
(2023)
Article
Chemistry, Multidisciplinary
Victor S. Batista, Renan L. Farias, Leonardo P. M. Simoes, Nailton M. Nascimento-Junior
Summary: This article discusses a simple and low-cost workflow for molecular docking evaluation, introducing how to use free software, web servers, and computa-tional tools supported by national funding agencies to construct, optimize, and dock bioactive substances in biomacromolecules.
Article
Biochemical Research Methods
Xiao Wang, Genki Terashi, Daisuke Kihara
Summary: DNA and RNA play vital roles in cellular processes, and understanding their functions relies on the knowledge of their three-dimensional structures. However, modeling the structures of DNA and RNA remains challenging, especially at resolutions lower than atomic level, and there is a lack of computational methods for nucleic acid structure modeling. In this study, a fully automated de novo DNA/RNA structure modeling method based on deep learning called CryoREAD is proposed. CryoREAD accurately identifies and models phosphate, sugar and base positions in cryo-EM maps, outperforming existing methods. The method was also applied to cryo-EM maps of SARS-CoV-2 biomolecular complexes.
