Article
Materials Science, Multidisciplinary
Constantine Tarabanis, David Miranda-Nieves, Thomas Ferrante, Carolyn A. Haller, Elliot L. Chaikof
Summary: Accurately measuring the thickness of biomaterial constructs is crucial for determining their mechanical properties. Various imaging modalities have been used, with Electron microscopy potentially leading to overestimation of mechanical properties. A standardized CLSM image acquisition approach has been developed to address these limitations and quantify thickness discrepancies in collagen-based scaffolds.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Multidisciplinary Sciences
Ana-Maria Pena, Etienne Decenciere, Sebastien Brizion, Peggy Sextius, Serge Koudoro, Therese Baldeweck, Emmanuelle Tancrede-Bohin
Summary: This study proposes an approach combining multiphoton FLIM and Pseudo-FLIM to quantify the 3D melanin distribution in vivo. By comparing the analyses of synthetic melanin, melanocytes/keratinocytes coculture, and in vivo human skin, new insights into melanin modulation are provided.
SCIENTIFIC REPORTS
(2022)
Article
Urology & Nephrology
Jesus Z. Villarreal, Javiera Perez-Anker, Susana Puig, Marc Xipell, Gerard Espinosa, Esther Barnadas, Ana B. Larque, J. Malvehy, Ricard Cervera, Arturo Pereira, Antonio Martinez-Pozo, Luis F. Quintana, Adriana Garcia-Herrera
Summary: This study demonstrates the feasibility of using ex vivo confocal microscopy for the analysis of basic patterns of lesion in nephropathology. FuCM proves to be as effective as conventional techniques in evaluating acute tubular necrosis and interstitial fibrosis changes, facilitating rapid evaluation of kidney biopsies and restructuring the clinical workflow in renal histopathology.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Nutrition & Dietetics
Jeong-Yong Park, Ji Yeon Lee, YongGyeong Kim, Chang-Ho Kang
Summary: UVB exposure leads to wrinkle formation, breakdown of collagen fiber, and transepidermal water loss. It induces the expression of MAPK, AP-1, and NF-kappa B, which impact the expression of MMP. The use of wikim0066 restores type I procollagen production and alleviates wrinkle formation in UVB-irradiated cells and mice.
Article
Dermatology
L. Tonellotto, T. Seremet, M. Vernez, E. Guenova, F. Kuonen
Summary: Through a comparison analysis of ex vivo CLSM and standard histopathology of TEN specimens, substantial agreement was found in the detection of subepidermal cleavage and confluent epidermal necrosis. Therefore, ex vivo CLSM appears to be a rapid, resource-optimizing, and reliable approach for emergency screening of TEN on fresh skin samples.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Stefania Guida, Federica Arginelli, Francesca Farnetani, Silvana Ciardo, Laura Bertoni, Marco Manfredini, Nicola Zerbinati, Caterina Longo, Giovanni Pellacani
Summary: Confocal laser scanning microscopy (CLSM) is a valuable tool in clinical settings, providing a quasi-histologic view of tissues without the need for biopsy. It has shown to improve diagnostic accuracy of skin cancers and contribute to reducing unnecessary excisions, as well as improving margin recognition for tissue sparing in excisions. Further studies using CLSM in real-life settings could enhance our understanding of its applications.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemical Research Methods
Imane Lboukili, Georgios Stamatas, Xavier Descombes
Summary: Reflectance confocal microscopy (RCM) allows for real-time visualization of the skin structure at the cellular level. In this study, an automated pipeline based on membrane detection was designed to identify and analyze epidermal cells in RCM images. The proposed method achieved higher accuracy compared to machine learning-based approaches.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Urology & Nephrology
Jesus Z. Villarreal, J. Perez-Anker, S. Puig, G. Pellacani, M. Sole, J. Malvehy, Luis F. Quintana, A. Garcia-Herrera
Summary: This study evaluated the feasibility of using ex vivo confocal microscopy in non-neoplastic kidney biopsies, and found that confocal images correlated well with conventional histological sections without introducing artifacts to the samples. The ease and speed of image acquisition, along with the quality of digitally stained images obtained, suggest promise for clinical applications in nephrology and renal transplantation.
JOURNAL OF NEPHROLOGY
(2021)
Article
Dermatology
Ke-Jun Chen, Zi-Yi Wang, Yang Han, Yong Cui
Summary: This study compared the clarity of imaging between multiphoton microscopy (MPM) and reflectance confocal microscopy (RCM) and measured the epidermal thickness at different body sites. Noninvasive tools were used to assess skin aging. The results showed that MPM had advantages in observing the stratum granulosum, collagen fiber, and elastic fiber, while RCM provided better observation in the dermo-epidermal junction layer. Epidermal thickness and SAAID scores correlated with age, gender, and body site.
SKIN RESEARCH AND TECHNOLOGY
(2023)
Article
Clinical Neurology
Peter D. Creigh, Khai Du, Elizabeth P. Wood, Joan Mountain, Janet Sowden, Julie Charles, Steffen Behrens-Spraggins, David N. Herrmann
Summary: This study establishes normal cutoff values for Meissner corpuscle densities adjusted for age, sex, and body dimensions. Increasing age leads to decreased sensitivity of touch-pressure and vibration thresholds. Sensitivity is higher in the hand compared to the leg or foot. Body dimensions and sex also affect touch-pressure thresholds and MC densities. These results are important for the detection and monitoring of peripheral sensory nerve disorders.
Article
Chemistry, Multidisciplinary
Yuhan Li, Milo S. P. Shaffer
Summary: This study demonstrates the novel use of confocal microscopy for high-resolution characterization, in situ laser reduction, and versatile patterning of graphene oxide (GO) films. The reduction process can be attributed to thermal transfer and accumulation, which can be controlled over multiple length scales. This technique allows for the fabrication of conductive reduced GO films and 3D microscale architectures, and provides a valuable tool for well-controlled and scalable fabrication.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Editorial Material
Medicine, General & Internal
Katharine Hanlon, Meredith Thomley, Lilia Correa-Selm
Summary: Demodex folliculorum and Demodex brevis can be detected on facial skin through Reflectance Confocal Microscopy (RCM), appearing as round groupings inside the sebaceous opening. While inflammation may occur, these mites are considered part of normal skin flora. The use of RCM to identify and study these mites may become more common as technology advances.
Article
Optics
Jingxi Li, Jason Garfinkel, Xiaoran Zhang, Di Wu, Yijie Zhang, Kevin de Haan, Hongda Wang, Tairan Liu, Bijie Bai, Yair Rivenson, Gennady Rubinstein, Philip O. Scumpia, Aydogan Ozcan
Summary: The traditional pathological diagnosis of skin tumors involves invasive biopsies and histological staining, which can be cumbersome and result in unnecessary biopsies and scarring. Emerging noninvasive optical technologies, like reflectance confocal microscopy (RCM), offer label-free, cellular-level imaging of skin without the need for a biopsy, but require specialized training due to grayscale images lacking nuclear features.
LIGHT-SCIENCE & APPLICATIONS
(2021)
Article
Ophthalmology
Ipek cigdem Ucar, Fehim Esen, Semra Akkaya Turhan, Halit Oguz, Hak Celal Ulasoglu, Veysel Aykut
Summary: Patients with irritable bowel syndrome (IBS) exhibit increased dry eye-associated ocular symptoms and nerve fiber loss in the corneal subbasal nerve plexus. IBS should be considered in the differential diagnosis when there is discrepancy between ocular discomfort levels and dry eye-associated corneal findings.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Norraseth Kaeokhamloed, Samuel Legeay, Emilie Roger
Summary: Advanced drug delivery system using nanocarriers is a major application of nanotechnology in pharmacotherapeutics. However, the clinical translation of nanomedicine faces challenges, and this review focuses on utilizing Fo•rster resonance energy transfer (FRET) as a method to track intact nanocarriers in vivo for studying pharmacokinetics.
JOURNAL OF CONTROLLED RELEASE
(2022)