Article
Biochemistry & Molecular Biology
Jun-Xue Jin, Jing-Tao Sun, Chao-Qian Jiang, Hong-Di Cui, Ya Bian, Sanghoon Lee, Lianjin Zhang, Byeong Chun Lee, Zhong-Hua Liu
Summary: This study reveals the role of melatonin receptors in lipid metabolism and redox signaling during in vitro cumulus-oocyte complex (COC) development, highlighting their potential to improve female reproductive health. Understanding the dynamics of interactions between lipid homeostasis and redox signaling driven by melatonin receptors is crucial for predicting drug targets and therapeutic effects.
Article
Reproductive Biology
Qiqi Li, Yaping Zhang, Wangchang Li, Ke Yan, Yaru Liu, Huiyan Xu, Yangqing Lu, Xingwei Liang, Xiaogan Yang
Summary: This study found that allicin can protect oocytes from LPS-induced damage and restore the normal function of the cells. These findings provide a theoretical basis for the use of plant-derived bioactive substances to improve oocyte maturation defects.
Article
Reproductive Biology
De-Cai Xiang, Bao-Yu Jia, Xiang-Wei Fu, Jian-Xiong Guo, Qiong-Hua Hong, Guo-Bo Quan, Guo-Quan Wu
Summary: The study demonstrated that Astaxanthin (Ax) significantly improved the survival rate of vitrified oocytes, promoted blastocyst yield, reduced reactive oxygen species generation, and increased glutathione level. Ax also decreased cathepsin B activity, increased lysosome fluorescence intensity, enhanced mitochondrial activity, and restored expression of specific genes in vitrified oocytes, ultimately improving developmental competence.
Article
Cell Biology
Lili Guo, Yongda Zhao, Yanjun Huan
Summary: The study showed that chlorpyrifos had toxic effects on porcine oocytes, including delaying meiotic progression, decreasing polar body extrusion, disrupting spindle assembly and causing DNA damage, as well as impairing cytoplasmic maturation. Pterostilbene was able to effectively alleviate the damage caused by chlorpyrifos and improve oocyte maturation and embryonic development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Xiaotong Luo, Xuan Chen, Yanqiu Lv, Yue Han, Xinglin Qu, Yuyang Zhang, Xin Li, Yongsheng Yu, Yi Jin
Summary: This study compared miRNA expression in porcine cumulus cells before and after oocyte maturation using RNA-seq technology and found that miR-101 regulates oocyte maturation by targeting HAS2.
Article
Pharmacology & Pharmacy
Qinyuan He, Xian Zhang, Xiaojun Yang
Summary: Beta-cypermethrin (8-CYP), a commonly used insecticide, has potential toxicity and adverse effects on the health of animals and humans. Our research found that 8-CYP exposure leads to defective nuclear maturation of porcine oocytes through abnormal spindle assembly and chromosome alignment. It also disrupts cytoplasmic maturation by affecting cortical granules, endoplasmic reticulum, and mitochondrial integrity, leading to increased reactive oxygen species and apoptosis. Supplementation with glutathione mitigates the meiotic defects induced by 8-CYP exposure by regulating ROS levels.
Article
Cell Biology
Antonella Mastrorocco, Ludovica Cacopardo, Letizia Temerario, Nicola Antonio Martino, Federico Tridente, Annalisa Rizzo, Giovanni Michele Lacalandra, Domenico Robbe, Augusto Carluccio, Maria Elena Dell'Aquila
Summary: In conventional assisted reproductive technologies, oocytes are cultured in vitro under static conditions. This study investigated a millifluidic in vitro oocyte maturation (mIVM) system, integrated with 3D printed supports, which better mimicked the physiological in vivo environment. Computational fluid dynamics and oxygen convection-reaction-diffusion models were used to model the system. The results showed that dynamic mIVM significantly reduced the maturation rate of oocytes and led to impaired mitochondria distribution and activity. However, when using specific 3D printed supports with alginate filling, oocyte maturation and mitochondria pattern were preserved, and bioenergetic/oxidative status was improved.
Article
Cell Biology
Song-Hee Lee, Ming-Hong Sun, Dongjie Zhou, Wen-Jie Jiang, Xiao-Han Li, Geun Heo, Xiang-Shun Cui
Summary: Exposure to high temperature has been found to negatively affect the development of porcine oocytes. It induces mitochondrial dysfunction, ER stress, abnormal Golgi apparatus distribution, and lysosomal damage, leading to the failure of meiotic maturation in the oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Pingping Zhang, Changyong Qi, Zhinan Ma, Yixiong Wang, Lei Zhang, Xiaojing Hou
Summary: This study reveals for the first time that in vivo exposure to PFOA impairs the meiotic maturation of oocytes in female mice, resulting in decreased rates of embryonic foam rupture and first polar body extrusion, disrupted mitochondrial metabolism, increased DNA damage, and abnormal spindle and chromosome arrangements.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Reproductive Biology
Yaping Zhang, Qiqi Li, Wangchang Li, Ke Yan, Yaru Liu, Huiyan Xu, Mingsheng Jiang, Yangqing Lu, Xingwei Liang, Jianghua Shang, Xiaogan Yang
Summary: This study found that 2-Mercaptoethanol (2-ME) can promote in vitro maturation of porcine oocytes by maintaining autophagy homeostasis, leading to improved embryo development.
Article
Reproductive Biology
Ashitha Suresh, M. K. Shukla, Dharmendra Kumar, O. P. Shrivastava, Neeraj Verma
Summary: The study shows that the simulated physiological oocyte maturation system significantly enhances the developmental competence of goat oocytes and reduces the intensity of reactive oxygen species and embryonic apoptosis.
Article
Reproductive Biology
Bethany K. Redel, Lee D. Spate, Ye Yuan, Clifton N. Murphy, R. Michael Roberts, Randall S. Prather
Summary: During in-vitro maturation of immature porcine oocytes, gonadotropins are not necessary when FGF2, LIF, and IGF1 are present. FLI directly impacts oocyte competence without the need for gonadotropins, and in the presence of gonadotropins, it enhances cumulus cell expansion.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Yue Wang, Chun-Hua Xing, Shun Chen, Shao-Chen Sun
Summary: The study showed that ZEN exposure disrupted organelle function in porcine oocyte meiotic maturation, affecting mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosome.
Article
Agriculture, Dairy & Animal Science
Pil-Soo Jeong, Hyo-Gu Kang, Bong-Seok Song, Sun-Uk Kim, Bo-Woong Sim, Sanghoon Lee
Summary: The present study aimed to investigate the restorative effects of SHH protein supplementation on the in vitro maturation (IVM) of low-quality porcine cumulus-oocyte complexes (COCs). The results showed that supplementation with SHH protein can improve the developmental competence of low-quality porcine COCs, indicating the importance of an active SHH signaling pathway for the acquisition of developmental competence in porcine COCs.
Article
Agriculture, Dairy & Animal Science
Huaxing Zhao, Xiaohua He, Xianjun Zhang, Junsong Shi, Rong Zhou, Ranbiao Mai, Qiaoyun Su, Gengyuan Cai, Sixiu Huang, Zheng Xu, Zhenfang Wu, Zicong Li
Summary: The quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, we found that progesterone and androstenedione were upregulated in the follicular fluid (FF) during in vivo pig oocyte maturation. The supplementation of progesterone or androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate and subsequent embryo developmental competence.