Insight into helicase mechanism and function revealed through single-molecule approaches

Tracking a Molecular Motor with a Nanoscale Optical Encoder

(2010) Charles E. Wickersham et al.   NANO LETTERS

The DEAD Box Helicase YxiN Maintains a Closed Conformation during ATP Hydrolysis

(2009) Regula Aregger et al.   BIOCHEMISTRY

PcrA Helicase Tightly Couples ATP Hydrolysis to Unwinding Double-Stranded DNA, Modulated by the Initiator Protein for Plasmid Replication, RepD

(2009) Andrew F. Slatter et al.   BIOCHEMISTRY

The mechanism of ATP-dependent RNA unwinding by DEAD box proteins

(2009) Manuel Hilbert et al.   BIOLOGICAL CHEMISTRY

Studying RecBCD Helicase Translocation Along χ-DNA Using Tethered Particle Motion with a Stretching Force

(2009) Hsiu-Fang Fan et al.   BIOPHYSICAL JOURNAL

BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation

(2009) Jaya G Yodh et al.   EMBO JOURNAL

Investigation of Stoichiometry of T4 Bacteriophage Helicase Loader Protein (gp59)

(2009) Sri Ranjini Arumugam et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Single-Molecule Analysis Reveals Differential Effect of ssDNA-Binding Proteins on DNA Translocation by XPD Helicase

(2009) Masayoshi Honda et al.   MOLECULAR CELL

Coordinating DNA replication by means of priming loop and differential synthesis rate

(2009) Manjula Pandey et al.   NATURE

Intersubunit coordination in a homomeric ring ATPase

(2009) Jeffrey R. Moffitt et al.   NATURE

Coupling DNA unwinding activity with primer synthesis in the bacteriophage T4 primosome

(2009) Maria Manosas et al.   Nature Chemical Biology

A conformational change in the helicase core is necessary but not sufficient for RNA unwinding by the DEAD box helicase YxiN

(2009) Anne R. Karow et al.   NUCLEIC ACIDS RESEARCH

Real-time single-molecule observation of rolling-circle DNA replication

(2009) Nathan A. Tanner et al.   NUCLEIC ACIDS RESEARCH

Type III restriction enzymes communicate in 1D without looping between their target sites

(2009) Subramanian P. Ramanathan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cytosolic Viral Sensor RIG-I Is a 5'-Triphosphate-Dependent Translocase on Double-Stranded RNA

(2009) S. Myong et al.   SCIENCE

Advances in Single-Molecule Fluorescence Methods for Molecular Biology

(2008) Chirlmin Joo et al.   Annual Review of Biochemistry

Translocation and Unwinding Mechanisms of RNA and DNA Helicases

(2008) Anna Marie Pyle   Annual Review of Biophysics

SnapShot: Nucleic Acid Helicases and Translocases

(2008) James M. Berger   CELL

On helicases and other motor proteins

(2008) Eric J Enemark et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding

(2008) Bo Sun et al.   EMBO JOURNAL

Establishing a Mechanistic Basis for the Large Kinetic Steps of the NS3 Helicase

(2008) Victor Serebrov et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Ferroplasma acidarmanus RPA2 Facilitates Efficient Unwinding of Forked DNA Substrates by Monomers of FacXPD Helicase

(2008) Robert A. Pugh et al.   JOURNAL OF MOLECULAR BIOLOGY

Dynamics of DNA replication loops reveal temporal control of lagging-strand synthesis

(2008) Samir M. Hamdan et al.   NATURE

A practical guide to single-molecule FRET

(2008) Rahul Roy et al.   NATURE METHODS

Non-hexameric DNA helicases and translocases: mechanisms and regulation

(2008) Timothy M. Lohman et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Single-molecule studies of fork dynamics in Escherichia coli DNA replication

(2008) Nathan A Tanner et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding

(2008) Ye Yang et al.   NUCLEIC ACIDS RESEARCH

The putative RNase P motif in the DEAD box helicase Hera is dispensable for efficient interaction with RNA and helicase activity

(2008) Martin H. Linden et al.   NUCLEIC ACIDS RESEARCH

DEAD-box proteins can completely separate an RNA duplex using a single ATP

(2008) Y. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cooperative binding of ATP and RNA induces a closed conformation in a DEAD box RNA helicase

(2008) B. Theissen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ATP hydrolysis is required for DEAD-box protein recycling but not for duplex unwinding

(2008) F. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA