Analysis of protein isoforms: Can we do it better?

Alternative Splice Variants, a New Class of Protein Cancer Biomarker Candidates: Findings in Pancreatic Cancer and Breast Cancer with Systems Biology Implications

(2013) Gilbert S. Omenn et al.   DISEASE MARKERS

Nano-LC FTICR Tandem Mass Spectrometry for Top-Down Proteomics: Routine Baseline Unit Mass Resolution of Whole Cell Lysate Proteins up to 72 kDa

(2012) Jeremiah D. Tipton et al.   ANALYTICAL CHEMISTRY

Automated Workflow for Large-Scale Selected Reaction Monitoring Experiments

(2012) Lars Malmström et al.   JOURNAL OF PROTEOME RESEARCH

Large-scale isotype-specific quantification of Serum amyloid A 1/2 by multiple reaction monitoring in crude sera

(2012) Hye-Jin Sung et al.   Journal of Proteomics

Characterization and stability of transthyretin isoforms in cerebrospinal fluid examined by immunoprecipitation and high-resolution mass spectrometry of intact protein

(2012) Keld Poulsen et al.   METHODS

Targeted Data Extraction of the MS/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis

(2012) Ludovic C. Gillet et al.   MOLECULAR & CELLULAR PROTEOMICS

Electron transfer dissociation mass spectrometry in proteomics

(2012) Min-Sik Kim et al.   PROTEOMICS

Induction and identification of disulfide-intact and disulfide-reduced β-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS and top-down proteomics

(2011) Clifton K. Fagerquist et al.   ANALYST

Microchip capillary electrophoresis–electrospray ionization–mass spectrometry of intact proteins using uncoated Ormocomp microchips

(2011) Tiina Sikanen et al.   ANALYTICA CHIMICA ACTA

High-resolution mass spectrometry for integrated qualitative and quantitative analysis of pharmaceuticals in biological matrices

(2011) Gérard Hopfgartner et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Tandem Mass Tag Protein Labeling for Top-Down Identification and Quantification

(2011) Chien-Wen Hung et al.   ANALYTICAL CHEMISTRY

Robust Analysis of the Yeast Proteome under 50 kDa by Molecular-Mass-Based Fractionation and Top-Down Mass Spectrometry

(2011) John F. Kellie et al.   ANALYTICAL CHEMISTRY

High Resolution Time-of-Flight Mass Analysis of the Entire Range of Intact Singly-Charged Proteins

(2011) Jeonghoon Lee et al.   ANALYTICAL CHEMISTRY

Chip-Type Asymmetrical Flow Field-Flow Fractionation Channel Coupled with Mass Spectrometry for Top-Down Protein Identification

(2011) Ki Hun Kim et al.   ANALYTICAL CHEMISTRY

Sensitive and Reproducible Intact Mass Analysis of Complex Protein Mixtures with Superficially Porous Capillary Reversed-Phase Liquid Chromatography Mass Spectrometry

(2011) Michael J. Roth et al.   ANALYTICAL CHEMISTRY

Performance Characteristics of a New Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer (TripleTOF 5600)

(2011) Genna L. Andrews et al.   ANALYTICAL CHEMISTRY

Identification and quantification of osteopontin splice variants in the plasma of lung cancer patients using immunoaffinity capture and targeted mass spectrometry

(2011) Jiang Wu et al.   BIOMARKERS

An Alternative Splicing Switch Regulates Embryonic Stem Cell Pluripotency and Reprogramming

(2011) Mathieu Gabut et al.   CELL

Alternative Spliced Variants as Biomarkers of Colorectal Cancer

(2011) Qian Yi et al.   CURRENT DRUG METABOLISM

Intact proteome fractionation strategies compatible with mass spectrometry

(2011) Alan A Doucette et al.   Expert Review of Proteomics

Fibronectin splice variants: Understanding their multiple roles in health and disease using engineered mouse models

(2011) Eric S. White et al.   IUBMB LIFE

Analysis of Intact Protein Isoforms by Mass Spectrometry

(2011) Jeremiah D. Tipton et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Intact protein separation by chromatographic and/or electrophoretic techniques for top-down proteomics

(2011) Anna Laura Capriotti et al.   JOURNAL OF CHROMATOGRAPHY A

Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I: Selection of chromatographic system and estimation of adsorption isotherms

(2011) Sivakumar Palani et al.   JOURNAL OF CHROMATOGRAPHY A

Analysis of peptides and proteins using sub-2μm fully porous and sub 3-μm shell particles

(2011) Aline Staub et al.   JOURNAL OF CHROMATOGRAPHY A

Human proteome analysis by using reversed phase monolithic silica capillary columns with enhanced sensitivity

(2011) Mio Iwasaki et al.   JOURNAL OF CHROMATOGRAPHY A

Weak anion exchange chromatographic profiling of glycoprotein isoforms on a polymer monolithic capillary

(2011) Jing Liu et al.   JOURNAL OF CHROMATOGRAPHY A

Intact protein analysis in the biopharmaceutical field

(2011) Aline Staub et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Ultra High Resolution Linear Ion Trap Orbitrap Mass Spectrometer (Orbitrap Elite) Facilitates Top Down LC MS/MS and Versatile Peptide Fragmentation Modes

(2011) Annette Michalski et al.   MOLECULAR & CELLULAR PROTEOMICS

Mapping intact protein isoforms in discovery mode using top-down proteomics

(2011) John C. Tran et al.   NATURE

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

(2011) Patrick J. M. Murphy et al.   Jove-Journal of Visualized Experiments

Size-Sorting Combined with Improved Nanocapillary Liquid Chromatography−Mass Spectrometry for Identification of Intact Proteins up to 80 kDa

(2010) Adaikkalam Vellaichamy et al.   ANALYTICAL CHEMISTRY

The late news on baff in autoimmune diseases

(2010) Pierre Youinou et al.   AUTOIMMUNITY REVIEWS

Towards reliable isoform quantification using RNA-SEQ data

(2010) Brian E Howard et al.   BMC BIOINFORMATICS

Splice variants of Enigma homolog, differentially expressed during heart development, promote or prevent hypertrophy

(2010) Tomoko Yamazaki et al.   CARDIOVASCULAR RESEARCH

Molecular Function of TCF7L2: Consequences of TCF7L2 Splicing for Molecular Function and Risk for Type 2 Diabetes

(2010) Ola Hansson et al.   Current Diabetes Reports

The actin gene family: Function follows isoform

(2010) Benjamin J. Perrin et al.   Cytoskeleton

Expression of neuregulin 4 splice variants in normal human tissues and prostate cancer and their effects on cell motility

(2010) N. V. L. Hayes et al.   ENDOCRINE-RELATED CANCER

Intact-protein trapping columns for proteomic analysis in capillary high-performance liquid chromatography

(2010) Xia Guan et al.   JOURNAL OF CHROMATOGRAPHY A

Depletion of highly abundant proteins in blood plasma by hydrophobic interaction chromatography for proteomic analysis

(2010) Andrea Mahn et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Role of Differential Expression of Interferon Receptor Isoforms on the Response of Multiple Sclerosis Patients to Therapy with Interferon Beta

(2010) Francesca Gilli   JOURNAL OF INTERFERON AND CYTOKINE RESEARCH

Native Electrospray and Electron-Capture Dissociation in FTICR Mass Spectrometry Provide Top-Down Sequencing of a Protein Component in an Intact Protein Assembly

(2010) Hao Zhang et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

A top-down LC-FTICR MS-based strategy for characterizing oxidized calmodulin in activated macrophages

(2010) Natacha Lourette et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Intact mass detection, interpretation, and visualization to automate Top-Down proteomics on a large scale

(2010) Kenneth R. Durbin et al.   PROTEOMICS

Multiplexed Size Separation of Intact Proteins in Solution Phase for Mass Spectrometry

(2009) John C. Tran et al.   ANALYTICAL CHEMISTRY

Splicing variants impact in thyroid normal physiology and pathological conditions

(2009) Elizabete Rosária de Miranda et al.   Arquivos Brasileiros de Endocrinologia e Metabologia

Comparison of methods for quantification of subtle splice variants

(2009) Stefanie Schindler et al.   ELECTROPHORESIS

On-line separation of native proteins by two-dimensional liquid chromatography using a single column

(2009) Xindu Geng et al.   JOURNAL OF CHROMATOGRAPHY A

A robust two-dimensional separation for top-down tandem mass spectrometry of the low-mass proteome

(2009) Ji Eun Lee et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

What does the future hold for top down mass spectrometry?

(2009) Benjamin A. Garcia   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Alternative Splicing in Lung Cancer

(2009) Ruben Pio et al.   Journal of Thoracic Oncology

Insulin-like growth factor-I E peptides: implications for ageing skeletal muscle

(2009) Cristina P. Velloso et al.   SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS

Thrombopoietin and its splicing variants: Structure and functions in thrombopoiesis and beyond

(2008) Roberto Marcucci et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

Multiple reaction monitoring for quantitative biomarker analysis in proteomics and metabolomics☆

(2008) Neil R. Kitteringham et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Nuanced roles of cytokines in three major human brain disorders

(2008) Lawrence Steinman   JOURNAL OF CLINICAL INVESTIGATION

The Neuregulin Family of Genes and their Multiple Splice Variants in Breast Cancer

(2008) Nandini V. L. Hayes et al.   JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA

New insights into form and function of fibronectin splice variants

(2008) ES White et al.   JOURNAL OF PATHOLOGY

Top-down identification and characterization of biomolecules by mass spectrometry

(2008) Kathrin Breuker et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Selected reaction monitoring for quantitative proteomics: a tutorial

(2008) Vinzenz Lange et al.   Molecular Systems Biology

Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

(2008) Qun Pan et al.   NATURE GENETICS