Article
Cell Biology
Sarah Rosli, Christopher M. Harpur, Maggie Lam, Alison C. West, Christopher Hodges, Ashley Mansell, Kate E. Lawlor, Michelle D. Tate
Summary: The pore-forming protein gasdermin D (GSDMD) plays a significant role in promoting damaging inflammation and the development of severe influenza A virus (IAV) disease. Deficiency in GSDMD reduces the severity of IAV disease, decreases neutrophil infiltration and pro-inflammatory cytokines, and improves survival outcomes. Additionally, GSDMD deficiency reduces viral burden and pulmonary pathology.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Francisco J. Zapatero-Belinchon, Rebecca Moeller, Lisa Lasswitz, Marco van Ham, Miriam Becker, Graham Brogden, Ebba Rosendal, Wenjie Bi, Belen Carriqui-Madronal, Koushikul Islam, Annasara Lenman, Antonia P. Gunesch, Jared Kirui, Thomas Pietschmann, Anna K. Overby, Lothar Jansch, Gisa Gerold
Summary: Clinical data suggests that statin therapy, particularly fluvastatin, may have a moderate beneficial effect on SARS-CoV-2 infection in human lung cells by downregulating proteins related to protein translation and viral replication, without affecting innate antiviral immune responses.
Article
Medicine, Research & Experimental
Laurence M. Black, Seth Winfree, Suraj D. Khochare, Malgorzata M. Kamocka, Amie M. Traylor, Stephanie K. Esman, Shehnaz Khan, Abolfazl Zarjou, Anupam Agarwal, Tarek M. El-Achkar
Summary: Lymphangiogenesis (LA) is a process that occurs in response to tissue injury, with unclear roles in disease progression. The study focuses on the distribution and spatial dynamics of LA in the context of acute kidney injury. The lymphatic system is crucial in physiology and has been identified as a key player in disease progression.
LABORATORY INVESTIGATION
(2021)
Article
Cardiac & Cardiovascular Systems
Mingming Liu, Meng Yan, Jinlong He, Huizhen Lv, Zhipeng Chen, Liyuan Peng, Wenbin Cai, Fang Yao, Chen Chen, Lei Shi, Kai Zhang, Xu Zhang, Dao-Wen Wang, Li Wang, Yi Zhu, Ding Ai
Summary: Inhibition of MST1/2 exacerbates cardiac dysfunction post-MI by affecting macrophage subtype distribution and LTB4 production.
CIRCULATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Haojie Zhang, Xue Zhao, Yifei Gao, Yao Shi, Lina Wei, Jingxin Li, Chuanyong Liu, Xuelian Ma
Summary: This study found that D-mannose can promote the recovery of colitis and alleviate inflammation in both animal and cell models. The mechanisms involved increasing the expression of tight junction proteins, reducing intestinal permeability, inhibiting M1 macrophage polarization and promoting M2 macrophage polarization, and inducing AMPK phosphorylation.
Article
Cardiac & Cardiovascular Systems
Prakash Doddapattar, Rishabh Dev, Madankumar Ghatge, Rakesh B. Patel, Manish Jain, Nirav Dhanesha, Steven R. Lentz, Anil K. Chauhan
Summary: This study demonstrates that myeloid cell-specific PKM2 regulates macrophage efferocytosis and atherosclerosis. Deletion or inhibition of PKM2 reduces atherosclerotic lesions, suppresses inflammation, and enhances macrophage efferocytosis.
CIRCULATION RESEARCH
(2022)
Article
Immunology
Huahua Du, Li Liang, Jiahui Li, Qingqing Xiong, Xin Yu, Hong Yu
Summary: The study found that Lcn2 plays a protective role in alleviating systemic inflammation and is an essential factor for macrophage functions.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Immunology
Laura D. Manzanares-Meza, Claudia Gutierrez-Roman, Albertana Jimenez-Pineda, Felipe Castro-Martinez, Genaro Patino-Lopez, Eunice Rodriguez-Arellano, Ricardo Valle-Rios, Vianney F. Ortiz-Navarrete, Oscar Medina-Contreras
Summary: Mucosal innate immunity is the first line of defense against pathogens. IL-1 cytokines are important regulators in inflamed mucosa. The secretion of IL-1 cytokines is regulated by unconventional pathways. Mouse macrophages experiments indicate IL-36 gamma is mainly secreted through membrane pores formed by P2X7R and Gasdermin D.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Adithap Hansakon, Siranart Jeerawattanawart, Pornpimon Angkasekwinai
Summary: The cytokines IL-33 and IL-25, derived from epithelial cells, play important roles in driving type-2 inflammation during C. neoformans infection. However, the impact of these cytokines in regulating host T helper cell response during C. neoformans infection is still unclear. Our study showed that C. neoformans infection promoted an increase in T helper cells co-expressing IL-25 and IL-33 receptors in the lung during the late infection phase. Transcriptomic analysis revealed a cooperative effect of IL-25 and IL-33 in promoting IL-13 gene expression in effector T helper cells.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Jun Tian, Tieying Song, Hong Wang, Wenli Wang, Xiaojing Ma, Yue Hu
Summary: The study investigated the potential therapeutic effects of a TLR2 antagonist CU-CPT22 on diabetic neuropathy in T2DM mice. Results showed that CU-CPT22 could prevent pro-inflammatory cytokine production, repolarize macrophages to M2 phenotype, suppress inflammation, and ameliorate neuropathic pain caused by diabetic neuropathy.
NEUROCHEMICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Suhrid Banskota, Huaqing Wang, Yun Han Kwon, Jaya Gautam, Sabah Haq, Jensine Grondin, Gregory R. Steinberg, Waliul I. Khan
Summary: Inhibiting NOX2 can alleviate colitis independently of AMPK, as shown by the study.
Article
Cell Biology
Darine W. El-Naccache, Fei Chen, Mark J. Palma, Alexander Lemenze, Matthew A. Fischer, Wenhui Wu, Pankaj K. Mishra, Holger K. Eltzschig, Simon C. Robson, Francesco Di Virgilio, George S. Yap, Karen L. Edelblum, Gyorgy Hasko, William C. Gause
Summary: Intestinal nematode parasites can cause tissue damage and promote host protective type 2 immunity by crossing the epithelial barrier and releasing danger-associated molecular patterns. The A2B adenosine receptor on intestinal epithelial cells plays an important role in this process. Blocking this receptor inhibits the host protective memory response to the parasite, affecting granuloma development at the host-parasite interface.
Article
Immunology
Vera Khodzhaeva, Yannick Schreiber, Gerd Geisslinger, Ralf P. Brandes, Bernhard Bruene, Dmitry Namgaladze
Summary: Deficiency of mitochondrial outer membrane GTPase Mitofusin 2 (MFN2) leads to decreased mitochondrial respiration, enhanced inflammatory responses, and increased interactions between endoplasmic reticulum and mitochondria. This deficiency potentiates the inflammatory signaling pathways triggered by zymosan in human macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Hematology
Juliana M. Navia-Pelaez, Colin Agatisa-Boyle, Soo-Ho Choi, Yi Sak Kim, Shenglin Li, Elena Alekseeva, Kimberly Weldy, Yury I. Miller
Summary: The plasma membrane of macrophages in atherosclerotic lesions may undergo reprogramming to maintain inflammarafts, which serve as platforms for assembling inflammatory receptor complexes.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Immunology
Jennifer Vandooren, Yoshifumi Itoh
Summary: Alpha-2-macroglobulin is known for its role as a broad-spectrum protease inhibitor and has additional functions such as regulating proteolysis, promoting cell migration, and binding cytokines, especially crucial in immune-cell function. This review provides an overview of all functions of Alpha-2-macroglobulin in the context of inflammation, immunity, and infections.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
J. A. Reales-Calderon, Z. Sun, V. Mascaraque, E. Perez-Navarro, V. Vialas, E. W. Deutsch, R. L. Moritz, C. Gil, J. L. Martinez, G. Molero
Summary: Pseudomonas aeruginosa is a significant opportunistic human pathogen, and the study of its proteome is crucial for uncovering virulence factors and antibiotic resistance mechanisms. The construction of proteomic resources like PeptideAtlas enables targeted proteomics studies, system-wide observations, and cross-species observational studies.
JOURNAL OF PROTEOMICS
(2021)
Article
Microbiology
Carmen Garcia-Duran, Raquel Martinez-Lopez, Ines Zapico, Enrique Perez, Eduardo Romeu, Javier Arroyo, Maria Luisa Hernaez, Aida Pitarch, Lucia Monteoliva, Concha Gil
Summary: The choice of stool sample processing and microbial cell disruption methods can affect metaproteomic analyses, with the use of a tube rotator and sonication increasing protein identification, while different disruption methods also impact the identification of various microbial taxa.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Catarina Vaz, Aida Pitarch, Emilia Gomez-Molero, Ahinara Amador-Garcia, Michael Weig, Oliver Bader, Lucia Monteoliva, Concha Gil
Summary: In this study, the hyphal secretome of C. albicans was characterized by gel-free proteomic analysis, and it was found that IC patients had higher antibody levels to these hyphal proteins, which may be useful for the development of future IC diagnostic tests.
Article
Infectious Diseases
Andres Ceballos-Garzon, Lucia Monteoliva, Concha Gil, Carlos Alvarez-Moreno, Nelson E. Vega-Vela, David M. Engelthaler, Jolene Bowers, Patrice Le Pape, Claudia M. Parra-Giraldo
Summary: This study identified the mechanism of caspofungin resistance, evaluated the proteomic response to caspofungin, and found that calmodulin inhibition can restore caspofungin susceptibility, reduce stress response, biofilm formation, and pathogenicity in vivo.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Immunology
Jose Antonio Reales-Calderon, Gloria H. W. Tso, Alrina S. M. Tan, Pei Xiang Hor, Julia Boehme, Karen W. W. Teng, Evan W. Newell, Amit Singhal, Norman Pavelka
Summary: Serial passaging of Candida albicans in the gastrointestinal tract of antibiotics-treated mice leads to the selection of virulence-attenuated strains that induce trained immunity, resulting in expansion of neutrophils and reduction of natural killer cells to protect the host. Metabolic changes and cell analysis further uncover the mechanisms underlying trained immunity induced by gut-evolved C. albicans strains.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Ahinara Amador-Garcia, Ines Zapico, Ana Borrajo, Johan Malmstrom, Lucia Monteoliva, Concha Gil
Summary: This study investigated the global proteomic changes in Candida albicans in response to stressors like hydrogen peroxide and acetic acid, providing insights for the development of new antifungal drugs.
Article
Microbiology
Norida Velez, Lucia Monteoliva, Zilpa-Adriana Sanchez-Quitian, Ahinara Amador-Garcia, Rocio Garcia-Rodas, Andres Ceballos-Garzon, Concha Gil, Patricia Escandon, Oscar Zaragoza, Claudia-Marcela Parra-Giraldo
Summary: This study found that the combination of iron and copper can enhance the pathogenicity of Cryptococcus neoformans and increase the abundance of proteins related to virulence factors. This suggests that the uptake of metals may affect the pathogenicity of fungi.
Review
Engineering, Biomedical
Maxine Lam, Jose Antonio Reales-Calderon, Jin Rong Ow, Giulia Adriani, Andrea Pavesi
Summary: Despite advancements in diagnosis and treatment, liver cancer remains a major global health concern, with a need for improved physiological relevance and reliability in in vitro models. Researchers are working towards better understanding cancer progression and treatment options, as well as replicating the complexity of the tumor microenvironment in vitro.
APL BIOENGINEERING
(2021)
Article
Microbiology
Raquel Martinez-Lopez, Maria Luisa Hernaez, Esther Redondo, Guillermo Calvo, Sonja Radau, Mercedes Pardo, Concha Gil, Lucia Monteoliva
Summary: This first analysis of HEVs of C. albicans has revealed distinct differences between them and the YEVs of C. albicans, indicating their importance and potential use in identifying new diagnostic markers and treatment targets for C. albicans infections. The data suggest different mechanisms of YEVs and HEVs biogenesis, as well as different roles in cell biology and host interaction. YEVs play a significant role in cell wall maintenance, while HEVs are more closely associated with virulence, exerting greater effects on human immune cells. Notably, an active 20S proteasome complex is exclusively secreted by HEVs. Further investigation into the functions of proteins exclusively found in HEVs could open up new research avenues in combating C. albicans.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Claudia Saralegui, Carmen Garcia-Duran, Eduardo Romeu, Maria Luisa Hernaez-Sanchez, Ainhize Maruri, Natalia Baston-Paz, Adelaida Lamas, Saioa Vicente, Estela Perez-Ruiz, Isabel Delgado, Carmen Luna-Paredes, Juan de Dios Caballero, Javier Zamora, Lucia Monteoliva, Concepcion Gil, Rosa del Campo
Summary: In recent years, various techniques have been used to study microbial ecosystems, with 16S rRNA gene sequencing being the most common method. Metaproteomics can identify the interaction between microorganisms and human cells, but the available databases are limited and imprecise. This study compares the differences between 16S rRNA amplicon sequencing and metaproteomics in identifying microorganisms and analyzing alpha diversity. Additionally, the study explores the establishment of intestinal ecosystems in newborns with cystic fibrosis, characterized by host inflammation and enrichment of Ruminococcus gnavus.
MICROBIOLOGY SPECTRUM
(2022)
Editorial Material
Immunology
Priya Uppuluri, Concha Gil, Jose Luis Lopez-Ribot
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Meeting Abstract
Biotechnology & Applied Microbiology
M. Morente-Lopez, R. Mato-Basalo, P. Miranda, J. Fafian-Labora, C. Gil, M. Carrera, M. C. Arufe
HUMAN GENE THERAPY
(2021)
Meeting Abstract
Biotechnology & Applied Microbiology
M. Morente-Lopez, R. Mato-Basalo, P. Miranda, J. Fafian-Labora, C. Gil, M. Carrera, M. C. Arufe
HUMAN GENE THERAPY
(2021)
