Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification

Unprecedented Rates and Efficiencies Revealed for New Natural Split Inteins from Metagenomic Sources

(2012) Patricia Carvajal-Vallejos et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Intramolecular Disulfide Bond between Catalytic Cysteines in an Intein Precursor

(2012) Wen Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Kinetic Control of One-Pot Trans-Splicing Reactions by Using a Wild-Type and Designed Split Intein

(2011) Neel H. Shah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Self-cleaving fusion tags for recombinant protein production

(2011) Yifeng Li   BIOTECHNOLOGY LETTERS

Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage

(2011) Wei Lu et al.   JOURNAL OF CHROMATOGRAPHY A

Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications

(2011) Brian P Callahan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Spontaneous Proton Transfer to a Conserved Intein Residue Determines On-Pathway Protein Splicing

(2010) Brian Pereira et al.   JOURNAL OF MOLECULAR BIOLOGY

Branched intermediate formation stimulates peptide bond cleavage in protein splicing

(2010) Silvia Frutos et al.   Nature Chemical Biology

Solution structure of DnaE intein fromNostoc punctiforme: Structural basis for the design of a new split intein suitable for site-specific chemical modification

(2009) Jesper S. Oeemig et al.   FEBS LETTERS

The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction

(2009) Joachim Zettler et al.   FEBS LETTERS

Splicing of the Mycobacteriophage Bethlehem DnaB Intein

(2009) Kazuo Tori et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family

(2009) Bareket Dassa et al.   NUCLEIC ACIDS RESEARCH

Modulation of intein activity by its neighboring extein substrates

(2009) G. Amitai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optimization of ELP-intein mediated protein purification by salt substitution

(2009) Baley A. Fong et al.   PROTEIN EXPRESSION AND PURIFICATION

Controllable protein cleavages through intein fragment complementation

(2009) Gerrit Volkmann et al.   PROTEIN SCIENCE