Article
Chemistry, Multidisciplinary
Kelly K. Miller, Sarah K. Springthorpe, Jennifer Imbrogno, David J. F. Walker, Shruti Gadiyar, Benjamin K. Keitz, Andrew D. Ellington, Nathaniel A. Lynd, Hal S. Alper
Summary: Research has shown that pyomelanin can serve as a biosourced material with excellent properties such as electron transport and ultraviolet light absorption, as well as superior performance in various applications. By metabolically engineering a strain of Yarrowia lipolytica, pyomelanin can be produced for use in areas such as polymer plasticization, demonstrating performance on par with or better than chemically sourced analogs.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Jolanta Malajowicz, Mariola Kozlowska
Summary: Yarrowia lipolytica yeast is used in environmentally friendly biotransformation reactions and can produce important compounds for the food and cosmetics industries. This study focused on biosynthesis of fat-derived fragrances gamma-decalactone (GDL) and hexanal, with parameters such as lipid substrate concentration and emulsification found to significantly impact production yield.
APPLIED SCIENCES-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Lu Liu, Jing Guo, Xue-Feng Zhou, Ze Li, Hai-Xiang Zhou, Wei-Qing Song
Summary: This study identified a novel tannase, TanA, with remarkable properties including high thermostability and multifunctionality, making it a potential candidate for food and agricultural processing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yixuan Wu, Yen-Han Lin
Summary: The redox potential control using potassium ferricyanide as an oxidant was found to enhance the production of PDO by Lactobacillus panis PM1. The addition of oxidant reduced fermentation time and resulted in co-substrate utilization of glucose and glycerol. This control strategy can benefit other PDO-producing strains and enhance their productivity.
PROCESS BIOCHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Francisco A. Guardiola, Maria angeles Esteban, Carlos Angulo
Summary: Yarrowia lipolytica has been industrially used for over two decades to produce docosahexaenoic acid and eicosapentaenoic acid, and is now gaining attention for its potential use as a functional feed additive for animals. Studies have shown that this yeast is safe, has probiotic and nutritional properties, and can enhance productive and immune parameters in various animals. Additionally, compounds derived from Y. lipolytica have shown improvements in productive performance, immune status, and disease resistance in animals, indicating promising opportunities for its future applications in the animal industry.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Food Science & Technology
Bruno Nicolau Paulino, Adones Sales, Lorena Felipe, Glaucia Maria Pastore, Gustavo Molina, Juliano Lemos Bicas
Summary: Aroma is a crucial attribute of food directly impacting consumer acceptance. In recent decades, interest in biotechnologically produced aroma compounds has grown as a sustainable way to provide natural additives for the food industry. Recent advances in microbial and enzymatic production of aroma compounds demonstrate their high potential for commercial applications.
CURRENT OPINION IN FOOD SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Xiangyu Zhang, Herong Wang, Qingsi Li, Zhaoxu Yin, Haishan Qi, Jing Yang, Xiaodong Wang, Wenhai Xiao, Lei Zhang
Summary: This study developed a new class of hydrophobic and lipophilic organogels for efficient encapsulation of oleaginous yeasts. The optimized organogel achieved a high viability of around 96% and showed potential for long-term storage by inhibiting cryoinjuries. The organogels also exhibited different temperature-dependent phase transition properties based on alkyl lengths, enabling rapid selection of targeted yeasts for steganography.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Biotechnology & Applied Microbiology
Young-Kyoung Park, Rodrigo Ledesma-Amaro
Summary: Yarrowia lipolytica is a promising candidate for industrial bioproduction of chemicals, fuels, foods, and pharmaceuticals, thanks to its natural and engineered traits. In recent years, extensive research has been conducted to assess its potential, improve its features, and scale up its processes. The increasing number of publications and patents related to Y. lipolytica demonstrates its importance and potential in the field. Despite the remaining challenges, it is expected that Y. lipolytica will become one of the preferred choices for industrial applications in the near future.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Patrycja Szczepanska, Magdalena Rychlicka, Pawel Moroz, Tomasz Janek, Anna Gliszczynska, Zbigniew Lazar
Summary: Phospholipids are a class of lipids with diverse biological functions and are commonly used in lipid replacement therapy. This study found that Yarrowia lipolytica is a promising producer of phospholipids, especially using waste substrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemical Research Methods
Andreea M. Palage, Valerie C. A. Ward
Summary: The production of hydrophobic or lipophilic compounds through biosynthesis in microbes is challenging due to their accumulation in intracellular hydrophobic microenvironments. This can result in cytotoxic effects, leading to the cessation of biomolecule production or even cell death. This article discusses strategies that have been developed in recent years to engineer greater storage capacity in microbes producing hydrophobic compounds.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Steven A. van der Hoek, Matej Rusnak, Irene Hjorth Jacobsen, Jose L. Martinez, Douglas B. Kell, Irina Borodina
Summary: Ergothioneine, a natural antioxidant, was successfully produced using the oleaginous yeast Yarrowia lipolytica as a host, reaching a high production yield in fed-batch fermentation. This study demonstrates the potential of Y. lipolytica for efficient ergothioneine production, highlighting its promise for industrial applications.
Article
Agricultural Engineering
Wen-Yan Tang, Dong-Ping Wang, Yun Tian, Xiao Fan, Chong Wang, Xiang-Yang Lu, Pei-Wang Li, Xiao-Jun Ji, Hu-Hu Liu
Summary: The study successfully increased the production of squalene in Yarrowia lipolytica using pathway engineering and bioprocess engineering, achieving significant experimental results.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Teba Torres-Zapata, Patricia Lozano-Martinez, M. Victoria Martinez-Lorenzo, Ruben M. Buey, Nicolas Martin-Sanchez
Summary: The accumulation of plastic waste is a pressing issue that needs to be addressed urgently. This study proposes a method to convert the carbon content of plastic waste into triglycerides, with a focus on polyethylene. The research demonstrates the feasibility of using polyethylene as a carbon source for fermentation, resulting in the production of triglycerides.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Veronika Urbanikova, Young-Kyoung Park, Daniela Krajciova, Mehdi Tachekort, Milan Certik, Ioana Grigoras, Roman Holic, Jean-Marc Nicaud, Peter Gajdos
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was used as a host for the production of Punicic acid (PuA). By supplementing the medium with pomegranate seed oil, lipids containing PuA esters were accumulated up to 31.2% in Y. lipolytica. Strategies involving the expression of the bifunctional fatty acid conjugase/desaturase gene from Punica granatum (PgFADX) allowed for de novo production of PuA in Y. lipolytica. The optimized promoter for PgFADX expression resulted in a PuA production of 36.6 mg/L. These findings highlight the potential of Y. lipolytica as a promising host for PuA production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Zedi Wang, Linlin Zhou, Minrui Lu, Yuwei Zhang, Samina Perveen, Huarong Zhou, Zhiqiang Wen, Zhaoxian Xu, Mingjie Jin
Summary: Yarrowia lipolytica strain was evolved through adaptive laboratory evolution to increase tolerance to ferulic acid, leading to a new strain with improved viability under high concentrations of ferulic acid, showing promise for application in lignocellulose conversion.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
