Article
Biology
Constance Kleijwegt, Florent Bressac, Coline Seurre, Wilhelm Bouchereau, Camille Cohen, Pascale Texier, Thomas Simonet, Laurent Schaeffer, Patrick Lomonte, Armelle Corpet
Summary: Promyelocytic leukemia nuclear bodies (PML NBs) play a crucial role in genome function by regulating the distribution of HIRA and histone dynamics. Additionally, they regulate the transcription of interferon-stimulated genes (ISGs) and H3.3 deposition mediated by HIRA during inflammatory response.
Review
Virology
Boris Ryabchenko, Vojtech Sroller, Lenka Hornikova, Alexey Lovtsov, Jitka Forstova, Sandra Huerfano
Summary: This review provides detailed descriptions of the diverse and complex interactions between small and medium size DNA viruses and promyelocytic leukemia nuclear bodies (PM NBs). The interactions between PM NB components and viruses can influence viral genome expression and/or replication, as well as IFN-mediated or apoptotic cell responses to viral infection. The review also discusses the interactions between PM NBs and Hepatitis B virus, papillomaviruses, polyomaviruses, and avian anellovirus. Despite numerous studies on the functions of PM NBs in the context of viral infection, there are still gaps in our understanding of the fine interactions between viruses and the highly dynamic PM NBs, which require further investigation.
Article
Biology
Myriam Scherer, Clarissa Read, Gregor Neusser, Christine Kranz, Anna K. Kuderna, Regina Mueller, Florian Full, Sonja Woerz, Anna Reichel, Eva-Maria Schilling, Paul Walther, Thomas Stamminger
Summary: PML nuclear bodies undergo rearrangement to form giant cages upon viral infection, trapping viral genomes and capsids, and providing a multilayered defense strategy against viral infections.
Article
Multidisciplinary Sciences
William Villiers, Audrey Kelly, Xiaohan He, James Kaufman-Cook, Abdurrahman Elbasir, Halima Bensmail, Paul Lavender, Richard Dillon, Borbala Mifsud, Cameron S. Osborne
Summary: The PML-RARA gene fusion is the characteristic driver of Acute Promyelocytic Leukaemia (APL) and is known to bind to the genome. Here, the authors characterise the impact of PML-RARA on gene regulation in APL cell lines and patient samples using transcriptomics, epigenomics, and machine learning.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Hsiao P. J. Voon, Linda Hii, Andrew Garvie, Maheshi Udugama, Brian Krug, Caterina Russo, Anderly C. Chueeh, Roger J. Daly, Alison Morey, Toby D. M. Bell, Stephen J. Turner, Joseph Rosenbluh, Paul Daniel, Ron Firestein, Jeffrey R. Mann, Philippe Collas, Nada Jabado, Lee H. Wong
Summary: The point mutations in H3.3 found in pediatric gliomas disrupt the formation of PML nuclear bodies and prevent differentiation. Similar to leukemias, glioma cells with these mutations are sensitive to drugs that target PML bodies.
Article
Multidisciplinary Sciences
Seishiro Hirano, Osamu Udagawa
Summary: Promyelocytic leukemia (PML) proteins play a role in the development of acute promyelocytic leukemia (APL) and can be affected by trivalent arsenic (As3+). The exposure to As3+ can lead to changes in PML-NBs, including solubility shift, SUMOylation, and late agglomeration. Understanding these changes is important for the regulation of intranuclear dynamics of PML-NBs.
Article
Plant Sciences
Karolina Kolarova, Martina Nespor Dadejova, Tomas Loja, Gabriela Lochmanova, Eva Sykorova, Martina Dvorackova
Summary: The disruption of the H2A-H2B histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) can suppress the loss-of-function phenotype of FAS1, leading to wild-type growth, decreased sensitivity to genotoxic stress, and suppression of telomere and 45S rDNA loss in Arabidopsis thaliana. This study demonstrates the essential role of NAP1 proteins in DNA repair in the absence of functional CAF-1, which is coupled to nucleosome assembly through modulation of H3 levels in the nucleus.
Review
Virology
Behdokht Jan Fada, Eleazar Reward, Haidong Gu
Summary: ND10, also known as PML-NBs, are dynamic membraneless subnuclear domains involved in key cellular processes such as DNA damage response, transcription regulation, apoptosis, oncogenesis, and antiviral defenses.
Article
Biochemistry & Molecular Biology
Hui Yang, Pinpin Sui, Ying Guo, Shi Chen, Marie E. Maloof, Guo Ge, Francine Nihozeko, Caroline R. Delma, Ganqian Zhu, Peng Zhang, Zhenqing Ye, Edward A. Medina, Nagi G. Ayad, Ruben Mesa, Stephen D. Nimer, Cheng-Ming Chiang, Mingjiang Xu, Yidong Chen, Feng-Chun Yang
Summary: This study reveals that deletion of Brd4 affects the self-renewal and differentiation of HSCs, leading to cell cycle arrest and senescence. The deletion of Brd4 results in increased chromatin accessibility and upregulation of senescence-specific genes. However, re-expression of BRD4 can rescue these effects by reducing H3 clipping and suppressing senescence gene expression.
Article
Immunology
Songbai Yang, Huaijin Liu, Zhenyu Chen, Han Wang, Xiangchen Li, Xiaolong Zhou, Ayong Zhao
Summary: This study investigates how Japanese encephalitis virus (JEV) evades immunity by disrupting the structure of PML-nuclear bodies (PML-NBs) through interaction with PML isoforms, potentially attenuating the host's antiviral immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Oncology
Mohammad B. Aljazi, Yuen Gao, Yan Wu, George Mias, Jin He
Summary: The study reveals the critical role of ASH1L in promoting MLL-AF9-induced leukemogenesis, showing that its loss impairs the maintenance and progression of leukemia cells. The enzymatic activity of ASH1L is necessary for promoting MLL-AF9-induced leukemic transformation.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Jaemin Eom, Kyuheum Jeon, Jung Sun Park, Yong-Kook Kang
Summary: SETDB1 is a histone methyltransferase that can be found in both the nucleus and cytoplasm. Its localization is primarily regulated by a balance between nuclear localization signals (NLS) and nuclear export signals (NES). Research findings suggest that cytoplasmic localization is a result of weak NLS and strong NES. In the presence of ATF7IP, SETDB1 enters the nucleus through a specific NES motif. The interaction between SETDB1 and ATF7IP in the nucleus helps maintain balanced levels of SETDB1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Plant Sciences
Aline V. Probst
Summary: This article discusses how modifying histone variants can regulate chromatin accessibility, establish functional chromatin states, and transmit chromatin states during replication. Overall, it emphasizes the importance of histone variants in maintaining chromatin structure and stability, as well as reprogramming epigenetic information.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Oncology
Wenjuan Ma, Yan Wan, Jianxiang Zhang, Jianan Yao, Yifei Wang, Jinchang Lu, Hong Liu, Xiaorui Huang, Xiuyan Zhang, Haixia Zhou, Yulong He, Depei Wu, Jianrong Wang, Yun Zhao
Summary: This study reveals that GAS2 promotes T-cell leukemogenesis through its interaction with CXCR4 to activate NOTCH1/c-MYC pathway. Loss of GAS2 has a mild effect on normal hematopoiesis.
MOLECULAR ONCOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Xiaodong Cheng
Summary: Protein SUMOylation is an essential post-translational modification for maintaining cellular homeostasis. Recent evidence suggests that liquid-liquid phase separation (LLPS)/ biomolecular condensates play a role in regulating cellular SUMOylation, providing insights into its cellular mechanism.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Review
Biochemical Research Methods
Qin Tang, Ronald M. Evans
Summary: Bile acids play significant roles in nutrient absorption, metabolic and immune homeostasis in the intestine. They are ligands for multiple nuclear receptors, regulating target genes and maintaining lipid balance. Gut microbiota biotransforms host-produced BAs diversifying the intestinal BA pool and promoting host-microbiota cross-talk.
EXPLORING NUCLEAR RECEPTORS
(2021)
Review
Biochemistry & Molecular Biology
Ronald M. Evans, Zong Wei
Summary: Pancreatic beta cells secrete insulin in response to glucose, and this process is regulated by multiple factors, including input signals from other organ systems. Impaired beta cell function is involved in the development of type 2 diabetes, and enhancing beta cell function through inter-organ communication, such as GLP-1 signaling, has been an effective therapeutic strategy.
Article
Multidisciplinary Sciences
Nasiha S. Ahmed, Jovylyn Gatchalian, Josephine Ho, Mannix J. Burns, Nasun Hah, Zong Wei, Michael Downes, Ronald M. Evans, Diana C. Hargreaves
Summary: This study found that the noncanonical BAF complex (ncBAF), a variant of the SWI/SNF complex, regulates the expression of secondary-response genes in the interferon response pathway. The BRD9 subunit of the ncBAF complex plays a specific role in the activation of interferon-stimulated genes (ISGs). Inhibition of BRD9 led to a reduction in ISG expression following endotoxin stimulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Multidisciplinary Sciences
Yanhong Shi, Ronald M. Evans, Fred H. Gage
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Endocrinology & Metabolism
Vincent Giguere, Ronald M. Evans
Summary: This review highlights the landmark discovery of the retinoic acid receptor (RAR) and subsequent studies that have enriched our understanding of the molecular mechanisms by which a low-abundant dietary compound is essential for life.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2022)
Article
Microbiology
Noemie Pied, Coralie F. Daussy, Zoe Denis, Jessica Ragues, Muriel Faure, Richard Iggo, Mario P. Tschan, Benoit Roger, Fabienne Rayne, Harald Wodrich
Summary: Intracellular pathogens cause membrane damage and cells respond by activating autophagy. The mechanism of autophagy induction during viral infections is rarely studied. This study investigates the cellular response to membrane damage during adenoviral entry and demonstrates that TBK1 kinase plays a crucial role in promoting autophagy in response to membrane penetration by adenoviruses. TBK1 is rapidly activated and recruited to the penetration sites, and its recruitment depends on damage recognition via galectin 8. This work highlights the importance of TBK1 in the cellular response to membrane damage and the activation of autophagy.
Article
Gastroenterology & Hepatology
Suzanne R. Sharpton, Tae Gyu Oh, Egbert Madamba, Chenjingyi Wang, Ruth T. Yu, Annette R. Atkins, Tao Huan, Michael Downes, Michael R. Evans, Rohit Loomba
Summary: This study provides evidence for a gut metagenome-derived signature with high diagnostic accuracy for predicting hepatic decompensation and mortality risk in patients with NAFLD-related cirrhosis.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Multidisciplinary Sciences
Vera J. M. Nies, Dicky Struik, Sihao Liu, Weilin Liu, Janine K. Kruit, Michael Downes, Tim van Zutphen, Henkjan J. Verkade, Ronald M. Evans, Johan W. Jonker
Summary: This study found that FGF1 can increase glucose uptake in adipocytes by activating GLUT4 protein. Furthermore, prolonged exposure to FGF1 can stimulate adipocyte glucose uptake by transcription of GLUT1 protein.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Gastroenterology & Hepatology
Daniel Q. Huang, Michael Downes, Ronald M. Evans, Joseph L. Witztum, Christopher K. Glass, Rohit Loomba
Summary: The global burden of nonalcoholic fatty liver disease (NAFLD) is increasing, with individuals with NAFLD at higher risk for cardiovascular disease, sharing multiple disease mechanisms.
SEMINARS IN LIVER DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Yohei Abe, Eric R. Kofman, Maria Almeida, Zhengyu Ouyang, Filipa Ponte, Jasmine R. Mueller, Grisel Cruz-Becerra, Mashito Sakai, Thomas A. Prohaska, Nathanael J. Spann, Ana Resende-Coelho, Jason S. Seidman, Joshua D. Stender, Havilah Taylor, Weiwei Fan, Verena M. Link, Isidoro Cobo, Johannes C. M. Schlachetzki, Takao Hamakubo, Kristen Jepsen, Juro Sakai, Michael Downes, Ronald M. Evans, Gene W. Yeo, James T. Kadonaga, Stavros C. Manolagas, Michael G. Rosenfeld, Christopher K. Glass
Summary: This study found that RANK signaling can convert the NCoR/HDAC3 co-repressor complex into a co-activator of AP-1 and NF-kB target genes, promoting mouse osteoclast differentiation. Mechanistically, RANK signaling activates the transcriptional co-activator PGC1(3 and inhibits HDAC3 activity for acetylated histone H3. Non-coding RNAs Dancr and Rnu12 play important roles in RANKL-induced osteoclast differentiation.
Article
Multidisciplinary Sciences
Min Young Kim, Hyun Young Shin, Sung Chun Cho, Sohae Yang, Aseer Intisar, Hyeong Jung Woo, Youn-Suk Choi, Chang-Lim You, Jong-Sun Kang, Yun-Il Lee, Sang Chul Park, Kyungmoo Yea, Tae Gyu Oh, Michael Downes, Ronald M. Evans, Minseok S. Kim
Summary: This study demonstrates the potential of a silver electroceutical technology to treat sarcopenia. Through the use of a high-throughput electrical stimulation screening platform, specific electrical stimulation conditions were identified that improved muscle tissue characteristics in aged mice and human-derived aged muscle cells. This research provides insight into personalized bioelectronic medicine.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Corina E. Antal, Tae Gyu Oh, Stefan Aigner, En-Ching Luo, Brian A. Yee, Tania Campos, Herve Tiriac, Katherine L. Rothamel, Zhang Cheng, Henry Jiao, Allen Wang, Nasun Hah, Elizabeth Lenkiewicz, Jan C. Lumibao, Morgan L. Truitt, Gabriela Estepa, Ester Banayo, Senada Bashi, Edgar Esparza, Ruben M. Munoz, Jolene K. Diedrich, Nicole M. Sodir, Jasmine R. Mueller, Cory R. Fraser, Erkut Borazanci, David Propper, Daniel D. Von Hoff, Christopher Liddle, Ruth T. Yu, Annette R. Atkins, Haiyong Han, Andrew M. Lowy, Michael T. Barrett, Dannielle D. Engle, Gerard I. Evan, Gene W. Yeo, Michael Downes, Ronald M. Evans
Summary: This study identifies a druggable RNA-binding protein cascade mediated by super-enhancers that supports PDAC growth through enhanced mRNA translation. The cascade is driven by a super-enhancer associated with the RBP heterogeneous nuclear ribonucleoprotein F, which stabilizes protein arginine methyltransferase 1 to control the translational mediator ubiquitin-associated protein 2-like. These genes and the regulatory super-enhancer are essential for PDAC growth and are coordinately regulated by Myc oncogene.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Ting Fu, Tao Huan, Gibraan Rahman, Hui Zhi, Zhenjiang Xu, Tae Gyu Oh, Jian Guo, Sally Coulter, Anupriya Tripathi, Cameron Martino, Justin L. Mccarville, Qiyun Zhu, Fritz Cayabyab, Brian Low, Mingxiao He, Shipei Xing, Fernando Vargas, Ruth T. Yu, Annette Atkins, Christopher Liddle, Janelle Ayres, Manuela Raffatellu, Pieter C. Dorrestein, Michael Downes, Rob Knight, Ronald M. Evans
Summary: Colorectal cancer is driven by genomic alterations and dietary influences, and the gut microbiome and metabolome play important roles in disease progression. A multi-omics study on mice reveals that diet is the major driver of microbiome and metabolome differences, with high-fat diet causing decreased diversity and changes in cecal metabolites.
Review
Endocrinology & Metabolism
Emanuel Gasser, Gencer Sancar, Michael Downes, Ronald M. Evans
Summary: FGF1 expression in adipose tissue and brain is involved in the regulation of metabolism. It can respond to dietary stress and regulate adipose tissue plasticity, and has anorexigenic properties. Recombinant FGF1 and variants with reduced mitogenicity have the potential to lower glucose, suppress adipose lipolysis, and promote insulin sensitization, making them candidates for the treatment of type 2 diabetes and associated comorbidities.
Article
Virology
Floriane Lagadec, Irene Carlon-Andres, Jessica Ragues, Sarah Port, Harald Wodrich, Ralph H. Kehlenbach
Summary: After receptor-mediated endocytosis, adenoviral capsids travel to the nuclear envelope via microtubule organizing centers. Capsid disassembly and viral genome import into nuclei involve nuclear pore complexes and the nucleoporins Nup214 and Nup358. The nuclear export receptor CRM1 is required for this process and can promote capsid disassembly even in mitotic cells, indicating an export-independent role. Additionally, inhibition of CRM1 by leptomycin B leads to a blockage of capsid movement, and a mutant CRM1 variant, W142A P143A, shows deficiency in capsid disassembly but is still capable of nuclear export of cargo proteins.
JOURNAL OF VIROLOGY
(2022)
