Article
Biochemistry & Molecular Biology
Pedro Martin, Melisa Moncada, Karen Castillo, Federico Orsi, Geronimo Ducca, Jose Manuel Fernandez-Fernandez, Carlos Gonzalez, Veronica Milesi
Summary: Arachidonic acid (AA) directly activates BK channels co-expressed with the auxiliary beta 1 subunit, stabilizing the voltage sensor domain (VSD) on its active configuration and facilitating channel opening transitions when VSD are at rest and CSD are unoccupied. The activation is independent of intracellular Ca2+ concentration and reduced when co-expressed with the Y74A mutant of the beta 1 subunit.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Review
Cardiac & Cardiovascular Systems
Thais S. Barenco-Marins, Fernando A. C. Seara, Cristiano G. Ponte, Jose H. M. Nascimento
Summary: The BK channel plays a significant role in regulating pulmonary vascular tonus by opening under specific conditions and causing smooth muscle relaxation. Dysfunction of the BK channel has been associated with the development of pulmonary hypertension (PH). Targeting the BK channel with selective agonists has shown promise in attenuating the progression of PH without adverse effects in animal models.
CARDIOVASCULAR DRUGS AND THERAPY
(2023)
Article
Neurosciences
Kelsey C. North, Anna N. Bukiya, Alex M. Dopico
Summary: Pregnenolone constricts middle cerebral arteries independently of neuronal, glial, endothelial, and circulating factors, as well as cell integrity, organelles, membrane cytoarchitecture, and cytosolic signals, through direct inhibition of smooth muscle BK channels.
Review
Physiology
Sara V. Ochoa, Liliana Otero, Andres Felipe Aristizabal-Pachon, Fernando Hinostroza, Ingrid Carvacho, Yolima P. Torres
Summary: Hypoxia is characterized by reduced cellular oxygen levels, triggering cell-signaling cascades, oxidative stress, activation of pro-inflammatory molecules, and influencing various cardiovascular diseases. The BK channel plays a central role in oxygen sensing mechanisms, with modifications of its properties involved in physiological functions and response to hypoxia.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anna N. Bukiya, Alex M. Dopico
Summary: The study shows that a high-CLR diet can protect cerebral arteries from alcohol-induced constriction by antagonizing the inhibition of BK channels and that this protection is dependent on cell integrity and a PKC-dependent mechanism.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Biochemistry & Molecular Biology
Steven C. Mysiewicz, Sydney M. Hawks, Anna N. Bukiya, Alex M. Dopico
Summary: Calcium/voltage-activated potassium channels (BK) control smooth muscle tone and cerebral artery diameter. Beta(1) subunits provide recognition sites for aldosterone at low and high concentrations, enabling BK activation and dilation of middle cerebral artery (MCA). The involvement of BK and identification of subunits in aldosterone's cerebrovascular action have not been investigated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Liang Sun, Frank T. Horrigan
Summary: This study defines mechanisms and interaction pathways that link voltage sensors to BK channel pores, providing insights into the key role of BK channels in integrating voltage and calcium signals.
Article
Geriatrics & Gerontology
Chao Xia, Yonghui Wang, Tianyuan Jiang, Yan Hu, Yang Chen, Xinrun Ma, Xuemei Zhang, Yanhong Gao
Summary: The study found that Slo1 deficiency impairs skeletal muscle regeneration and slow-twitch fiber formation.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Limnology
Ben Christopher Howard, Ian Baker, Nicholas Kettridge, Sami Ullah, Stefan Krause
Summary: This study evaluates the effects of sample storage duration and temperature on the concentrations of resazurin (raz) and resorufin (rru), finding that freezing samples provides the best preservation with maximum changes of around 10% after 14 days compared to maximum changes of around 30% when cooled. It is recommended to store raz/rru samples cooled for up to 48 hours and frozen for up to 14 days.
LIMNOLOGY AND OCEANOGRAPHY-METHODS
(2022)
Article
Pharmacology & Pharmacy
Kelsey C. North, Man Zhang, Aditya K. Singh, Dasha Zaytseva, Alexandria Slayden, Anna N. Bukiya, Alex M. Dopico
Summary: This study reveals that inhibition of calcium/voltage-gated BK channels by membrane cholesterol requires physiological levels of internal calcium (Ca2+) and is selectively linked to high-affinity Ca2+-sensing sites located in the cytosolic tail domain.
MOLECULAR PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Zhekai Jin, Tao Chen, Yuncong Liu, Wenwen Feng, Lili Chen, Chao Wang
Summary: In this paper, a reversible ion-dipole interaction with high-temperature stability exceeding 150°C is realized. The resulting supramolecular network possesses a mechanical strength of 1.32 MPa, dynamic self-healing capability, and a stable working temperature of up to 200°C. These remarkable performances are achieved through a new molecular design that balances high enthalpy with minimized entropy-dominated thermal dissociations.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Physical
Jiqiang Li, Yanzhi Ding, Hengheng Liu, Hua He, Daoyong Yu, Xiaoqiang Wang, Xiaojuan Wang, Xiaoxi Yu, Baosheng Ge, Fang Huang
Summary: This study utilized a dual-color simultaneous tracking system to visualize and quantitatively assess the interactions between CCR5 and CCL5 in real time, revealing that different oligomeric states of CCR5 have varying binding affinities with CCL5, with dimers showing a higher affinity than monomers. The dimerization of CCR5 may lead to asymmetric conformational changes, resulting in varied binding affinities for different binding pockets. This research provides insights into the stoichiometry of interactions between surface proteins at a single-molecule level.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Physical
Weihua Mu, Jing Kong, Jianshu Cao
Summary: This study investigates the conformation-modulated kinetics of human glucokinase using a five-state kinetic model, revealing the non-Michealis-Menten correction term of activity and the violation of conformational detailed balance. Furthermore, the study reproduces and explains the resonance effect observed in human glucokinase, and shows quantitative agreement with experimental measurements. The analysis suggests the generality of kinetic cooperativity and resonance in a general chemical network beyond the five-state model.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Analytical
Jingxin Liu, Weiwu Li, Rongsong Li, Xiuzhao Yin, Shiliang He, Junqing Hu, Shuangchen Ruan
Summary: This study developed programmable pH sensors using tetrahedral DNA framework and i-motif structures, allowing fine-tuning of response characteristics and enabling pH analysis in biological environments.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Taisuke Watanabe, Yutaka Kitamura, Hachidai Aizawa, Hideo Masuki, Tetsuhiro Tsujino, Atsushi Sato, Hideo Kawabata, Kazushige Isobe, Koh Nakata, Tomoyuki Kawase
Summary: This study optimized quantification conditions and applied DAPI probe to assess platelet polyP levels in a Japanese population. Results showed significantly higher polyP levels in women compared to men, with a moderate negative correlation between age and polyP levels in women. Additionally, women exhibited significantly higher responsiveness to CaCl2 stimulation than men. This DAPI-based protocol may serve as an efficient and cost-effective method for routine laboratory testing of platelet polyP levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Cardiac & Cardiovascular Systems
Peter Downey, William Ragalie, Vadim Gudzenko, Abbas Ardehali
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2019)
Editorial Material
Anesthesiology
Wolf B. Kratzert, Vadim Gudzenko
JOURNAL OF CARDIOTHORACIC AND VASCULAR ANESTHESIA
(2020)
Article
Neurosciences
Antonios Pantazis, Maki Kaneko, Marina Angelini, Federica Steccanella, Annie M. Westerlund, Sarah H. Lindstrom, Michelle Nilsson, Lucie Delemotte, Sulagna C. Saitta, Riccardo Olcese
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Medicine, General & Internal
Ryan P. Barbaro, Graeme MacLaren, Philip S. Boonstra, Theodore J. Iwashyna, Arthur S. Slutsky, Eddy Fan, Robert H. Bartlett, Joseph E. Tonna, Robert Hyslop, Jeffrey J. Fanning, Peter T. Rycus, Steve J. Hyer, Marc M. Anders, Cara L. Agerstrand, Katarzyna Hryniewicz, Rodrigo Diaz, Roberto Lorusso, Alain Combes, Daniel Brodie
Article
Biochemistry & Molecular Biology
Alexander Supady, Jeff Della Volpe, Fabio Silvio Taccone, Dominik Scharpf, Matthias Ulmer, Philipp M. Lepper, Maximilian Halbe, Stephan Ziegeler, Alexander Vogt, Raj Ramanan, David Boldt, Stephanie-Susanne Stecher, Andrea Montisci, Tobias Spangenberg, Olivier Marggraf, Chandra Kunavarapu, Lorenzo Peluso, Sebastian Muenz, Monica Buerle, Naveen G. Nagaraj, Sebastian Nuding, Catalin Toma, Vadim Gudzenko, Hans Joachim Stemmler, Federico Pappalardo, Georg Trummer, Christoph Benk, Guido Michels, Daniel Duerschmied, Constantin von zur Muehlen, Christoph Bode, Klaus Kaier, Daniel Brodie, Tobias Wengenmayer, Dawid L. Staudacher
Summary: The role of veno-venous extracorporeal membrane oxygenation therapy (V-V ECMO) in severe COVID-19 acute respiratory distress syndrome (ARDS) is still debated, and the use of clinical scores for predicting mortality in these patients undergoing V-V ECMO may not be recommended, but could be considered as an additional tool for prognosis evaluation.
Review
Engineering, Biomedical
Jeieung Park, Michael Y. Lin, Christopher L. Wray, Fady M. Kaldas, Peyman Benharash, Vadim Gudzenko
Summary: The study retrospectively analyzed the role and outcomes of extracorporeal life support (ECLS) in adult liver transplantation (LT) and conducted a literature review. The results showed that ECLS can be used as a bridge to LT or rescue therapy for respiratory failure with a survival rate of 44%. In patients with cardiac failure, the use of ECLS in the perioperative setting carries a high risk but may have a role in LT patients with a functioning graft and potentially reversible cardiac failure.
Article
Cardiac & Cardiovascular Systems
Yu Xia, William Ragalie, Eric H. Yang, Gentian Lluri, Reshma Biniwale, Peyman Benharash, Vadim Gudzenko, Rajan Saggar, David Sayah, Abbas Ardehali
Summary: This study compared the outcomes of venoarterial (VA) extracorporeal membrane oxygenation (ECMO) and venovenous (VV) ECMO as bridges to lung transplantation and found similar success rates and survival rates between the two modes.
ANNALS OF THORACIC SURGERY
(2022)
Article
Engineering, Biomedical
Jeffrey Javidfar, Ahmed Labib, Gabrielle Ragazzo, Ethan Kurtzman, Maria Callahan, Silver Heinsar, Vadim Gudzenko, Peter Barrett, Jose Binongo, Jane Wenjing Wei, John Fraser, Jacky Y. Suen, Gianluigi Li Bassi, Giles Peek
Summary: By adhering to strict precautions, patients with COVID-19 can be safely cannulated at an outside hospital and transported to a quaternary care center without increased risk to the transport team.
Article
Physiology
Marina Angelini, Arash Pezhouman, Nicoletta Savalli, Marvin G. Chang, Federica Steccanella, Kyle Scranton, Guillaume Calmettes, Michela Ottolia, Antonios Pantazis, Hrayr S. Karagueuzian, James N. Weiss, Riccardo Olcese
Summary: A new antiarrhythmic strategy based on selectively reducing late I-Ca,I-L shows promise in suppressing arrhythmias triggered by EADs while preserving myocardial function. This approach modifies the gating properties of Ca(V)1.2 channels rather than blocking their pore, thereby maintaining contractility.
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Article
Neurosciences
Teresa Minguez-Vinas, Varsha Prakash, Kaiqian Wang, Sarah H. Lindstrom, Serena Pozzi, Stuart A. Scott, Elizabeth Spiteri, David A. Stevenson, Euan A. Ashley, Cecilia Gunnarsson, Antonios Pantazis
Summary: Two variants of the KCNA2 gene were found in pediatric patients with epilepsy and developmental delay. These variants had opposite effects on neuronal excitability, with one variant increasing surface trafficking and activity of the channel, and the other variant reducing surface levels and inhibiting channel opening.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Critical Care Medicine
Gianluigi Li Bassi, Kristen Gibbons, Jacky Y. Suen, Heidi J. Dalton, Nicole White, Amanda Corley, Sally Shrapnel, Samuel Hinton, Simon Forsyth, John G. Laffey, Eddy Fan, Jonathon P. Fanning, Mauro Panigada, Robert Bartlett, Daniel Brodie, Aidan Burrell, Davide Chiumello, Alyaa Elhazmi, Mariano Esperatti, Giacomo Grasselli, Carol Hodgson, Shingo Ichiba, Carlos Luna, Eva Marwali, Laura Merson, Srinivas Murthy, Alistair Nichol, Mark Ogino, Paolo Pelosi, Antoni Torres, Pauline Yeung Ng, John F. Fraser
Article
Cardiac & Cardiovascular Systems
Joseph Hadaya, Yas Sanaiha, Vadim Gudzenko, Nida Qadir, Sumit Singh, Ali Nsair, Nam Yong Cho, Richard J. Shemin, Peyman Benharash
Summary: This study reports on the institutional experience with mobile extracorporeal life support (ECLS) for various indications. Patients were transported using ground ambulances from different institutions, and the clinical outcomes were promising.
Article
Multidisciplinary Sciences
Michelle Nilsson, Sarah H. Lindstrom, Maki Kaneko, Kaiqian Wang, Teresa Minguez-Vinas, Marina Angelini, Federica Steccanella, Deborah Holder, Michela Ottolia, Riccardo Olcese, Antonios Pantazis
Summary: We report a heterozygous KCNA2 variant in a child with epilepsy. The mutation causes substitution F233S at the voltage-sensing domain of the K(V)1.2 subunit. Our experiments show that the K(V)1.2(F233S) subunits have impaired trafficking and reduce the surface expression of wild-type K(V)1.2 and KV1.4, significantly disrupting electrical signaling. We found that the F233S mutation causes a depolarizing shift in voltage dependence of K(V)1.2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Surgery
Leigh C. Reardon, Jeannette P. Lin, Glen S. VanArsdell, Fady M. Kaldas, Gentian Lluri, Weiyi Tan, Katrina M. Whalen, Daniel Cruz, Ali Nsair, Mario C. Deng, Melissa A. Moore, Hillel Laks, Reshma M. Biniwale, Sammy Saab, Andrew Baird, James M. Wilson, Lorraine N. Lubin, Jure Marijic, Tiffany M. Williams, Christopher L. Wray, Joseph S. Meltzer, Vadim Gudzenko, Wolf B. Kratzert, Jacques Neelankavil, Robert S. Venick, Jamil A. Aboulhosn
Summary: Single ventricle physiology comprises a range of rare congenital cardiac abnormalities that require surgical intervention in infancy for long-term survival. The Fontan operation is associated with chronic central venous hypertension and multi-organ consequences. Heart and liver transplantation in Fontan patients present challenges but also opportunities for treatment.
CURRENT TRANSPLANTATION REPORTS
(2021)
Article
Critical Care Medicine
Alexander Supady, Fabio Silvio Taccone, Philipp M. Lepper, Stephan Ziegeler, Dawid L. Staudacher