Article
Biochemistry & Molecular Biology
Mariangela Centrone, Marianna Ranieri, Annarita Di Mise, Mariagrazia D'Agostino, Maria Venneri, Angela Ferrulli, Giovanna Valenti, Grazia Tamma
Summary: Renal collecting duct principal cells express water channels that mediate renal water reabsorption. Vasopressin stimulation activates specific signaling pathways and induces the translocation of AQP2 vesicles from the intracellular pool to the plasma membrane. AQP2 trafficking and function are regulated by post-translational modifications and several proteins involved in the redistribution of AQP2 vesicles.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Review
Medicine, General & Internal
Gheun-Ho Kim
Summary: Drug-induced hyponatremia caused by renal water retention is mainly classified into SIADH and NSIAD. Psychotropic agents, anticancer chemotherapeutic agents, and thiazide diuretics are the major causes. NSIAD, which involves the upregulation of AQP2, is the major mechanism for drug-induced hyponatremia.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Marianna Ranieri, Annarita Di Mise, Mariangela Centrone, Mariagrazia D'Agostino, Stine Julie Tingskov, Maria Venneri, Tommaso Pellegrino, Graziana Difonzo, Francesco Caponio, Rikke Norregaard, Giovanna Valenti, Grazia Tamma
Summary: The Olive Leaf Extract (OLE) has been shown to prevent the translocation of AQP2 induced by vasopressin, decrease the osmotic water permeability coefficient, and stimulate the Calcium Sensing Receptor (CaSR) in renal cells. In vivo experiments further demonstrated that OLE increases CaSR mRNA expression, decreases AQP2 mRNA expression, and increases miRNA-137 targeting AQP2, suggesting its potential to attenuate disorders involving abnormal CaSR signaling and renal water reabsorption.
SCIENTIFIC REPORTS
(2021)
Article
Physiology
Hsiu-Hui Yang, Shih-Han Su, Cheng-Hsuan Ho, Ai-Hsin Yeh, Yi-Jiun Lin, Ming-Jiun Yu
Summary: The expression of the water channel protein AQP2 is regulated by the glucocorticoid receptor and the pituitary hormone vasopressin. Transcriptomic comparison revealed positive and negative regulatory pathways maintained by the glucocorticoid receptor for the vasopressin-induced Aqp2 gene expression.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Junki Kurimoto, Hiroshi Takagi, Takashi Miyata, Yohei Kawaguchi, Yuichi Hodai, Tetsuro Tsumura, Daisuke Hagiwara, Tomoko Kobayashi, Yoshinori Yasuda, Mariko Sugiyama, Takeshi Onoue, Shintaro Iwama, Hidetaka Suga, Ryoichi Banno, Takeshi Katsuki, Fumiaki Ando, Shinichi Uchida, Hiroshi Arima
Summary: The symptoms of diabetes insipidus may be masked by adrenal insufficiency and appear after the administration of hydrocortisone, especially at high doses. Study found that high-dose corticosteroid treatment in the deficiency of arginine vasopressin (AVP) can induce polyuria, which is caused by the effects of dexamethasone or aldosterone on water balance and renal excretion.
Article
Urology & Nephrology
Kota Kakeshita, Tsutomu Koike, Teruhiko Imamura, Hayato Fujioka, Hidenori Yamazaki, Koichiro Kinugawa
Summary: AVP, cAMP, and AQP2 in the collecting ducts of the kidney are associated with fluid volume and serum osmolality regulation in patients with chronic kidney disease (CKD). Urine cAMP may be a promising marker for assessing residual function of the collecting duct.
CLINICAL AND EXPERIMENTAL NEPHROLOGY
(2022)
Article
Physiology
Sua Kim, Chor Ho Jo, Gheun-Ho Kim
Summary: This study demonstrates that antipsychotic drugs like haloperidol, sertraline, and carbamazepine can induce nephrogenic syndrome of inappropriate antidiuresis by upregulating vasopressin-2 receptor and aquaporin-2 (AQP2) through cAMP/PKA signaling. Additionally, these drugs also increase AQP2 protein abundance through dephosphorylation at Ser(261) and enhanced transcription.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Physiology
Grazia Tamma, Annarita Di Mise, Marianna Ranieri, Mariangela Centrone, Maria Venneri, Mariagrazia D'Agostino, Angela Ferrulli, Bostjan Simunic, Marco Narici, Rado Pisot, Giovanna Valenti
Summary: Exposure to actual or simulated microgravity leads to changes in renal function, fluid distribution, and bone loss. Through a study on healthy volunteers who underwent 10 days of bedrest, it was found that inactivity resulted in decreased vasopressin and AQP2 excretion, increased urinary calcium, decreased PTH, reduced urinary osteopontin, and increased adrenomedullin levels, indicating altered renal function and increased risk of stone formation.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Physiology
Cheng-Hsuan Ho, Hsiu-Hui Yang, Shih-Han Su, Ai-Hsin Yeh, Ming-Jiun Yu
Summary: Vasopressin regulates the water permeability of kidney collecting ducts by inducing the expression of aquaporin-2 through the interaction between alpha-actinin 4 and glucocorticoid receptor. This mechanism provides an important pathway for the regulation of water reabsorption in the kidney collecting ducts.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Physiology
Christina Ernstsen, Frederic H. Login, Anne-Sofie B. Schelde, Jacob R. Therkildsen, Jakob Moller-Jensen, Rikke Norregaard, Helle Praetorius, Lene N. Nejsum
Summary: This study found that the urinary concentration defect in patients with acute pyelonephritis may be related to abnormal cellular localization of AQP2. Pathogenic bacteria can induce membrane targeting of AQP2, and this effect does not depend on an increase in cAMP levels or phosphorylation of the S256 site.
Article
Endocrinology & Metabolism
Alba Ferre, Francois Chauvigne, Magdalena Gozdowska, Ewa Kulczykowska, Roderick Nigel Finn, Joan Cerda
Summary: The neurohypophysial and paracrine vasopressinergic systems regulate the trafficking of Aqp1ab-type paralogs via a common AVP-AVPR2AA-PKA pathway to avoid competitive occupancy of the same plasma membrane space and maximize water influx during oocyte hydration in marine teleosts.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hyo-Ju Jang, Hye-Jeong Park, Hong Seok Choi, Hyun Jun Jung, Tae-Hwan Kwon
Summary: This study aimed to establish mpkCCDc14 cells constitutively expressing V2R and AQP2 via CRISPR/Cas9-mediated genome engineering technology. The results showed that these cells had higher abundance of AQP2 protein in the absence of stimulation and exhibited normal response to dDAVP stimulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Junki Kurimoto, Hiroshi Takagi, Takashi Miyata, Yohei Kawaguchi, Yuichi Hodai, Tetsuro Tsumura, Daisuke Hagiwara, Tomoko Kobayashi, Yoshinori Yasuda, Mariko Sugiyama, Takeshi Onoue, Shintaro Iwama, Hidetaka Suga, Ryoichi Banno, Takeshi Katsuki, Fumiaki Ando, Shinichi Uchida, Hiroshi Arima
Summary: The mechanism underlying polyuria induced by high-dose corticosteroid therapy in patients with diabetes insipidus is not well understood. This study investigated the effects of aldosterone and dexamethasone on water balance in patients and mice models, and found that aldosterone treatment induced polyuria by reducing the expression of aquaporin-2 in the kidney.
Article
Urology & Nephrology
Federica Petrillo, Anna Iervolino, Tiziana Angrisano, Sabina Jelen, Vincenzo Costanzo, Mariavittoria D'Acierno, Lei Cheng, Qi Wu, Ilaria Guerriero, Maria Cristina Mazzarella, Alfonso De Falco, Fulvio D'Angelo, Michele Ceccarelli, Michele Caraglia, Giovambattista Capasso, Robert A. Fenton, Francesco Trepiccione
Summary: MiRNAs play a role in regulating the expression of AQP2 in the kidney collecting duct through epigenetic and transcriptional mechanisms.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Peripheral Vascular Disease
Azuma Nanamatsu, Takayasu Mori, Fumiaki Ando, Taisuke Furusho, Shintaro Mandai, Koichiro Susa, Eisei Sohara, Tatemitsu Rai, Shinichi Uchida
Summary: Desmopressin, a vasopressin type 2 receptor agonist, significantly increases short-term tubular uromodulin secretion in mice and reduces uromodulin abundance in the kidney. It activates uromodulin secretion in kidney epithelial cells through vasopressin/cAMP signaling, dependent on epithelial cell polarity and cyclic AMP-dependent PKA pathway. This discovery of vasopressin/cAMP/PKA signaling as a physiological stimulus for urinary uromodulin secretion may lead to novel treatment strategies for urinary tract infections, kidney stones, hypertension, and chronic kidney disease.
Article
Biochemical Research Methods
Duangnapa Kovanich, Chonticha Saisawang, Potchaman Sittipaisankul, Suwipa Ramphan, Nuttiya Kalpongnukul, Poorichaya Somparn, Trairak Pisitkun, Duncan R. Smith
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Biochemical Research Methods
Ting-Chun Pan, Chieh-Wen Lo, Weng Man Chong, Chia-Ni Tsai, Kuan-Ying Lee, Pin-Yin Chen, Jung-Chi Liao, Ming-Jiun Yu
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Virology
Chia-Ni Tsai, Ting-Chun Pan, Cho-Han Chiang, Chun-Chiao Yu, Shih-Han Su, Ming-Jiun Yu
JOURNAL OF VIROLOGY
(2019)
Article
Physiology
Shu-Ting Lin, Chia-Ching Ma, Kuang-Ting Kuo, Yin-Fang Su, Wei-Ling Wang, Tzu-Hsien Chan, Shih-Han Su, Shih-Che Weng, Chian-Huei Yang, Shuei-Liong Lin, Ming-Jiun Yu
FRONTIERS IN PHYSIOLOGY
(2019)
Article
Virology
Cho-Han Chiang, Yen-Ling Lai, Yu-Ning Huang, Chun-Chiao Yu, Christine C. Lu, Guann-Yi Yu, Ming-Jiun Yu
JOURNAL OF VIROLOGY
(2020)
Article
Engineering, Chemical
Somphot Saoin, Chatikorn Boonkrai, Trairak Pisitkun, Chiraphat Kloypan, Sawitree Nangola
Summary: NGAL is considered a promising biomarker for early prediction of acute kidney injury. This study successfully expressed recombinant NGAL protein under a Tet-On inducible system, providing an efficient strategy for producing other recombinant proteins secreted from mammalian cells.
Letter
Oncology
Nut Pipatpanyanugoon, Nicha Wareesawetsuwan, Sunisa Prasopporn, Wannapan Poolex, Trairak Pisitkun, Worasak Kaewkong, Somponnat Sampattavanich, Siwanon Jirawatnotai
CANCER COMMUNICATIONS
(2022)
Article
Virology
Chun-Chiao Yu, Pei-Chen Lin, Cho-Han Chiang, Shu-Tang Jen, Yen-Ling Lai, Shih-Chin Hsu, Lee-Chiang Lo, Jing-Jer Lin, Nei-Li Chan, Ming-Jiun Yu
Summary: In this study, we discovered for the first time that the ATP-binding domain of NS3 is involved in NS5A phosphorylation. In vitro assays showed that casein kinase I alpha is a very potent kinase responsible for NS5A phosphorylation at serines 225, 232, and 235. Our data suggest that ATP binding by NS3 probably results in conformational changes that recruit casein kinase I alpha to phosphorylate NS5A, initially at S225 and subsequently at S232 and S235. Our discovery reveals intricate requirements of the structural integrity of NS3 for NS5A hyperphosphorylation and HCV replication.
JOURNAL OF VIROLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Piyanoot Fonghem, Trairak Pisitkun, Kasem Rattanapinyopituk, Sirintra Sirivisoot, Anudep Rungsipipat
Summary: This study aimed to identify differential serum protein expressions of canine B- and T-cell lymphomas compared with healthy dogs using quantitative proteomics. The findings revealed potential lymphoma biomarkers in dogs and highlighted the need for further research with a larger sample size.
Article
Biochemical Research Methods
Nuttiya Kalpongnukul, Rungnapa Bootsri, Piriya Wongkongkathep, Pornchai Kaewsapsak, Chaiyaboot Ariyachet, Trairak Pisitkun, Naphat Chantaravisoot
Summary: This study investigated the abnormal mTORC2 signaling pathway in GBM cells and found that mTORC2 inhibition might be involved in DNA repair and cancer cell survival. Additionally, it was discovered that mTORC2 controls the phosphorylation of γH2AX to repair DNA. These findings provide important insights into the function of mTORC2 in oncogenic DNA damage response and offer potential mTORC2 treatments for brain cancer patients.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jiradej Makjaroen, Pornpimol Phuengmaung, Wilasinee Saisorn, Suwasin Udomkarnjananun, Trairak Pisitkun, Asada Leelahavanichkul
Summary: Lipopolysaccharide (LPS) tolerance, which reduces the immune response to repeated LPS stimulation, may contribute to sepsis-induced immune exhaustion. Macrophages with LPS tolerance showed decreased energy, reduced protein abundance, and down-regulated anti-viral genes. In asymptomatic mice, LPS tolerance was associated with higher viral abundance in the cecum and decreased expression of anti-viral genes, suggesting it could lead to viral reactivation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Sumonwan Chamsuwan, Chollada Buranakarl, Kris Angkanaporn, Thasinas Dissayabutra, Natthaya Chuaypen, Trairak Pisitkun, Nuttiya Kalpongnukul
Summary: This study found differences in urinary proteomic profiles between dogs with hypercalciuric CaOx urolithiasis and hypercalciuric stone-free dogs, suggesting that urinary proteomic profiles may serve as potential biomarkers for urinary tract injury in CaOx urolithiasis in dogs.
Article
Chemistry, Analytical
Pornchanok Punnoy, Tatiya Siripongpreda, Trairak Pisitkun, Nadnudda Rodthongkum, Pranut Potiyaraj
Summary: A novel lab-on-paper screening method using gold nanoparticles and laser desorption ionization-mass spectrometry (LDI-MS) was developed for rapid diagnosis of the SARS-CoV-2 Omicron BA.2 variant. The method allows for sensitive detection of SARS-CoV-2 antigen and can be used for quantitation of the antigen in saliva without the need for sample preparation. This platform could potentially be used for non-invasive diagnosis of the Omicron BA.2 variant.
Article
Physiology
Kit Yee Wong, Wei-Ling Wang, Shih-Han Su, Chin-Fu Liu, Ming-Jiun Yu
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2020)
Article
Physiology
Wei-Ling Wang, Shih-Han Su, Kit Yee Wong, Chan-Wei Yang, Chin-Fu Liu, Ming-Jiun Yu
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2020)