Article
Physiology
Sara R. Roig, Silvia Cassinelli, Andre Zeug, Evgeni Ponimaskin, Antonio Felipe
Summary: Members of the regulatory Kv beta family play a role in modulating the kinetics and traffic of voltage-dependent Kv channels. The formation of heteromeric complexes between Kv beta subtypes influences the spatial distribution in lipid rafts and can alter the immune response. Differentially regulating Kv beta subtypes can modulate Kv beta-dependent physiological responses and the architecture of Kv beta heterotetramers.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Physical
Sheeba Malik, Smarajit Karmakar, Ananya Debnath
Summary: All-atom molecular dynamics simulations reveal a significant growth in the heterogeneity length scales of interface water (IW) in 1,2-dimyristoyl-sn-glycero-3-phosphocholine lipid membranes across fluid to ripple to gel phase transitions. IW acts as an alternate probe to capture the ripple size of the membrane and follows an activated dynamical scaling within the gel phase. These results quantify the mostly unknown correlations between the spatiotemporal scales of IW and membranes at various phases under physiological and supercooled conditions.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Dan Qiao, Himanshu Joshi, Huangtianzhi Zhu, Fushi Wang, Yang Xu, Jia Gao, Feihe Huang, Aleksei Aksimentiev, Jiandong Feng
Summary: Synthetic nanopores have been developed for applications in membrane filtration technologies and biomolecular sensing, but challenges remain in providing them with atomically defined shape and deliberate placement of chemical groups. A chemically defined transmembrane nanopore, EPM, demonstrates stable transmembrane transport functionality, particularly with up to 21-fold selectivity for potassium over sodium ions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Cell Biology
Danila Bobkov, Svetlana Semenova
Summary: This review examines the role of lipid rafts in regulating TRP channel activity, with a focus on the interaction between the channels and cholesterol. Understanding the mechanisms behind these interactions would enhance our understanding of TRP channel regulation and allow for better control of cellular responses to environmental stimuli.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Claire Poulet, Jose Sanchez-Alonso, Pamela Swiatlowska, Florence Mouy, Carla Lucarelli, Anita Alvarez-Laviada, Polina Gross, Cesare Terracciano, Steven Houser, Julia Gorelik
Summary: JPH2 promotes T-tubule structural stability and recruits functional LTCCs to the membrane, most likely by directly binding to the channel. Cholesterol is involved in the binding of JPH2 to T-tubules as well as in the modulation of LTCC activity.
CARDIOVASCULAR RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Catia Santos-Pereira, Joana P. Guedes, Debora Ferreira, Ligia R. Rodrigues, Manuela Corte-Real
Summary: The milk-derived bovine lactoferrin (bLf) has selective anticancer activity against highly metastatic cancer cells with V-ATPase at the plasma membrane. It co-localizes with V-ATPase and cholesterol-rich lipid rafts, perturbs cellular trafficking, induces cholesterol accumulation and disrupts lipid rafts, downregulates PI3K and AKT/p-AKT, and inhibits glycolysis of cancer cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Yoshimi Oka
Summary: Membrane-anchored proteins and their mimics, such as PNAs, show selective partitioning into lipid raft or non-raft domains based on their lipophilic anchors. In this study, anchor-matched PNA was found to be excluded from the lipid raft domains of giant unilamellar vesicles when hybridized with complementary flavin-labeled DNA. The distribution of PNA was not only dependent on the lipophilic anchor, but also on the structure of the hybridized DNA or PNA pair.
Article
Chemistry, Organic
Tristan Wegner, Anna L. L. Matos, Karine Porte, Kira Mehring, Marco Pierau, Hendrik Horstmeier, Volker Gerke, Frank Glorius
Summary: Cholesterol is a vital lipid in cellular membranes and plays a role in various cellular processes. Diseases like immunological disorders and cancer have been associated with abnormalities in cholesterol homeostasis. Thus, there is a need for molecular tools to study cholesterol distribution and interactions. In this study, a novel bifunctional imidazolium-based cholesterol analog (X-CHIM) was synthesized, which can serve as a versatile tool for investigating cellular cholesterol distributions and cholesterol-protein interactions.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Sergio Serrano-Buitrago, Monica Munoz-Ubeda, Victor G. Almendro-Vedia, Juan Sanchez-Camacho, Beatriz L. L. Maroto, Florencio Moreno, Jorge Banuelos, Inmaculada Garcia-Moreno, Ivan Lopez-Montero, Santiago de la Moya
Summary: A simple, small and symmetric molecular probe based on BODIPY has been developed as a bright, photostable and functionalizable probe for plasma membrane (PM). By incorporating lateral polar ammoniostyryl groups, the probe's amphiphilicity and lipid membrane partitioning were increased. This new BODIPY probe demonstrated decreased diffusion across lipid bilayers compared to its precursor and showed optical capabilities in the red region for bioimaging.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Biochemistry & Molecular Biology
Danila Bobkov, Natalia Yudintceva, Ekaterina Lomert, Alla Shatrova, Lyudmila Kever, Svetlana Semenova
Summary: The destruction of lipid rafts affects cell adhesion and migration, leading to decreased cell speed and trajectory length. Through protein interaction network analysis, it is found that TRPV6 calcium channels and Src family kinase members have a close relationship in lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2021)
Article
Medicine, Research & Experimental
Andrei N. Tsentsevitsky, Chulpan R. Gafurova, Alexey M. Petrov
Summary: The study found that KATP channels have specific features in regulating neurotransmitter release in different regions and depend on endogenous ROS levels and lipid raft integrity. Activation of KATP channels suppresses the action of extracellular adenosine and ATP on evoked release by increasing ROS production.
Article
Biochemistry & Molecular Biology
Yuliya Berson, Sofia Khaitlina, Olga Tsaplina
Summary: Cell membrane rafts are important in controlling protein-protein and lipid-protein interactions on the cell surface. Disrupting membrane rafts by M beta CD reduced the intensity of Serratia invasion. This reduction was more significant in M-HeLa cells and correlated with faster assembly of the actin cytoskeleton. Treatment with M beta CD increased the invasion of S. proteamaculans in Caco-2 cells and correlated with increased EGFR expression. Overall, this study highlights the role of membrane rafts in bacterial invasion and suggests that targeting rafts could be a potential strategy to reduce bacterial invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mohammed F. Gholam, Lauren P. Liu, Louis A. Searcy, Nancy D. Denslow, Abdel A. Alli
Summary: The SGLT2 inhibitor dapagliflozin may improve comorbidities associated with type 2 diabetes mellitus by altering lipid composition, suppressing extracellular vesicle release, and disrupting lipid rafts in the kidney.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
D. W. Allender, M. Schick
Summary: Rafts in the plasma membrane are nanoscopic domains that provide stable platforms for proteins. The microemulsion theory suggests that the interaction between rafts depends on the size of the intervening sea, which can either attract or repel rafts, contributing to the stability of the platform.
EUROPEAN PHYSICAL JOURNAL E
(2023)
Article
Food Science & Technology
Hideo Kataoka, Ayumi Saeki, Akira Hasebe, Ken-ichiro Shibata, Takeshi Into
Summary: The study demonstrates that naringenin inhibits inflammatory responses triggered by TLR2, possibly by regulating the functioning of cell surface TLR2. This suggests a novel anti-inflammatory property of naringenin through modulation of TLR2 signaling.
FOOD SCIENCE & NUTRITION
(2021)