Article
Biochemistry & Molecular Biology
Jiangtao Ren, Xuhua Zhang, Zhenhui Zhang, Jiafeng Pan, Zhexue Hao, Jin Li, Jun Liu
Summary: The complex and costly manufacturing process of CAR-T cell therapy has hindered its widespread adoption. Induced pluripotent stem cells (iPSCs) have potential as an alternative cellular immunotherapy due to their unlimited self-renewal potential and ability to differentiate into functional immune cells. Careful selection of the original cell for iPSC seed preparation is crucial due to the presence of epigenetic imprint. Our research shows that lymphocyte-origin cells are superior to embryonic stem cells in differentiating into functional immune cells. Blocking Fas-FasL induced apoptosis in T cells significantly improves iPSC generation without affecting their differentiation potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Endocrinology & Metabolism
Ila Tewari Jasra, Nerea Cuesta-Gomez, Kevin Verhoeff, Braulio A. Marfil-Garza, Nidheesh Dadheech, A. M. James Shapiro
Summary: This review summarizes the roles and mechanisms of mitochondria in somatic cell reprogramming to iPSCs and the metabolic shift associated with directed differentiation into pancreatic beta-like cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Genetics & Heredity
Huajin Li, Wenting Xu, Sijia Xiang, Leiting Tao, Wen Fu, Jinhui Liu, Wenbin Liu, Yamei Xiao, Liangyue Peng
Summary: Pluripotency is a transient state in early embryos regulated by a network of pluripotency-related genes. This study identified four pluripotent genes in zebrafish and crucian carp and verified that three of them can be used as markers for pluripotency in induced pluripotent stem cells in fish.
FRONTIERS IN GENETICS
(2022)
Article
Computer Science, Interdisciplinary Applications
Slo-Li Chu, Kazuhiro Sudo, Hideo Yokota, Kuniya Abe, Yukio Nakamura, Ming -Dar Tsai
Summary: This study proposes a method using several deep learning models to efficiently select the reprogramming cells possibly forming hiPSCs and to predict the shapes of growing hiPSC colonies, with the aim of improving the efficiency when establishing a new hiPSC line culture.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2023)
Article
Cell Biology
Shogo Sato, Tomoaki Hishida, Kenichiro Kinouchi, Fumiaki Hatanaka, Yumei Li, Quy Nguyen, Yumay Chen, Ping H. Wang, Kai Kessenbrock, Wei Li, Juan Carlos Izpisua Belmonte, Paolo Sassone-Corsi
Summary: Distinct metabolic conditions in pluripotent stem cells (PSCs) are connected to clock functions through the circadian repressor CRY1. Metabolic reprogramming and the accumulation of CRY1 are essential for self-renewal capacity, colony organization, and metabolic signatures in PSCs. CRY1 regulates developmental and differentiation genes in PSCs, and its absence impairs induced PSC (iPSC) reprogramming efficiency.
Review
Immunology
Niclas Flosdorf, Martin Zenke
Summary: Novel methods of generating dendritic cells (DC) through reprogramming somatic cells have opened up exciting avenues for research and clinical applications. Induced pluripotent stem cells (iPS cells) and direct reprogramming of cells can both be used to generate DC.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Engineering, Biomedical
Isamu Ogawa, Daichi Onozato, Shiho Anno, Hisato Hayashi, Tatsuro Kanaki, Takahiro Iwao, Tamihide Matsunaga
Summary: This study proposes a new culture method for human induced pluripotent stem cell-derived intestinal organoids (HIOs) using low concentrations of natural polysaccharides in a suspension culture. The HIOs cultured in this method showed similarities to living intestines and exhibited pharmacokinetic functions. This method can be widely applied to drug discovery, disease research, and regenerative medicine, overcoming challenges in traditional culture methods.
Article
Cell & Tissue Engineering
Nelio A. J. Oliveira, Handan Sevim
Summary: Dendritic cells are important cells in the immune system, and researchers are looking for efficient methods to obtain dendritic cells from human induced pluripotent stem cells. Despite some challenges, progress has been made and there is potential for clinical applications.
STEM CELLS AND DEVELOPMENT
(2022)
Review
Biochemistry & Molecular Biology
Hyun Kyu Kim, Tae Won Ha, Man Ryul Lee
Summary: Cells are the basic units of organisms, with over 30 trillion cells generated from a single-cell fertilized egg through division and differentiation. Establishing the Human Cell Atlas aims to understand the mechanisms underlying diseases and cellular activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sergey A. Sinenko, Andrey A. Kuzmin, Elena V. Skvortsova, Sergey V. Ponomartsev, Evgeniya V. Efimova, Michael Bader, Natalia Alenina, Alexey N. Tomilin
Summary: In this study, the researchers investigated the impact of endogenous serotonin on reprogramming to pluripotency and found that serotonin synthesis has a negative effect on somatic cell reprogramming.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Aleksei G. Menzorov
Summary: Human and mouse induced pluripotent stem cells (PSCs) are widely used for studying early embryonic development and for modeling of human diseases. The review focuses on the technical aspects of deriving PSCs from Carnivora species and summarizes current data on dog, feline, ferret, and American mink PSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Tomoki Murata, Naoki Hama, Tomoki Kamatani, Akihiro Mori, Ryo Otsuka, Haruka Wada, Ken-ichiro Seino
Summary: This study investigated the immune regulation in transplantation using allogeneic induced pluripotent stem cells (iPSCs). The researchers found that minor antigen mismatches are a risk factor for graft rejection, highlighting the importance of immune regulation. They demonstrated that iPSC-derived hematopoietic stem/progenitor cells (iHSPCs) can induce allograft tolerance, providing a new approach for transplantation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Multidisciplinary Sciences
Jingyang Guan, Guan Wang, Jinlin Wang, Zhengyuan Zhang, Yao Fu, Lin Cheng, Gaofan Meng, Yulin Lyu, Jialiang Zhu, Yanqin Li, Yanglu Wang, Shijia Liuyang, Bei Liu, Zirun Yang, Huanjing He, Xinxing Zhong, Qijing Chen, Xu Zhang, Shicheng Sun, Weifeng Lai, Yan Shi, Lulu Liu, Lipeng Wang, Cheng Li, Shichun Lu, Hongkui Deng
Summary: This study demonstrates the chemical reprogramming of human somatic cells to human chemically induced pluripotent stem cells, which exhibit key features of embryonic stem cells. The induction of an intermediate plastic state and inhibition of the JNK pathway are crucial for successful chemical reprogramming.
Review
Dermatology
Satoshi Fukushima, Azusa Miyashita, Haruka Kuriyama, Toshihiro Kimura, Satoru Mizuhashi, Yosuke Kubo, Satoshi Nakahara, Hisashi Kanemaru, Nobuhiro Tsuchiya, Hiroaki Mashima, Rong Zhang, Yasushi Uemura
Summary: Cancer immunotherapy is the primary treatment for unresectable cancers, but it is not a complete cure for all patients. Immune cell therapy, specifically using immune cells generated from induced pluripotent stem cells (iPSCs), shows promise for innovative cancer immunotherapy methods. iPSC-derived dendritic cells (iPS-DCs) can activate T cells, while iPSC-derived macrophages (iPS-MPs) attack cancer cells. iPSCs offer a source for genetic modification and addition of immune functions. Additionally, immortalized iPS-DCs and iPS-MPs could potentially reduce costs through mass production. This review summarizes the achievements and future prospects of cancer research using iPS-DCs and iPS-MPs.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Cell & Tissue Engineering
Kazuki Morita, Akihiro Nakamura, Masakazu Machida, Tomoyuki Kawasaki, Rina Nakanishi, Justin Ichida, Takanori Iwata, Akihiro Umezawa, Hidenori Akutsu
Summary: Chemical inhibition of the NOTCH signaling pathway and DOT1L promotes the generation of hiPSCs from keratinocytes and significantly increases iPSC colony generation from human fibroblast cells via mRNA reprogramming. The user-friendly reprogramming approach using a single-cell sorting system under efficient conditions opens up possibilities for industrial applications in disease modeling and drug screening.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Kotaro Sasaki, Shihori Yokobayashi, Tomonori Nakamura, Ikuhiro Okamoto, Yukihiro Yabuta, Kazuki Kurimoto, Hiroshi Ohta, Yoshinobu Moritoki, Chizuru Iwatani, Hideaki Tsuchiya, Shinichiro Nakamura, Kiyotoshi Sekiguchi, Tetsushi Sakuma, Takashi Yamamoto, Takahide Mori, Knut Woltjen, Masato Nakagawa, Takuya Yamamoto, Kazutoshi Takahashi, Shinya Yamanaka, Mitinori Saitou
Article
Endocrinology & Metabolism
Eisaku Mori, Junji Fujikura, Michio Noguchi, Kazuhiro Nakao, Masaki Matsubara, Masakatsu Sone, Daisuke Taura, Toru Kusakabe, Ken Ebihara, Takayuki Tanaka, Kiminori Hosoda, Kazutoshi Takahashi, Isao Asaka, Nobuya Inagaki, Kazuwa Nakao
METABOLISM-CLINICAL AND EXPERIMENTAL
(2016)
Review
Cell Biology
Kazutoshi Takahashi, Shinya Yamanaka
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Hayami Sugiyama, Kazutoshi Takahashi, Takuya Yamamoto, Mio Iwasaki, Megumi Narita, Masahiro Nakamura, Tim A. Rand, Masato Nakagawa, Akira Watanabe, Shinya Yamanaka
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Tomonaga Ameku, Daisuke Taura, Masakatsu Sone, Tomohiro Numata, Masahiro Nakamura, Fumihiko Shiota, Taro Toyoda, Satoshi Matsui, Toshikazu Araoka, Tetsuhiko Yasuno, Shin-Ichi Mae, Hatasu Kobayashi, Naoya Kondo, Fumiyo Kitaoka, Naoki Amano, Sayaka Arai, Tomoko Ichisaka, Norio Matsuura, Sumiko Inoue, Takuya Yamamoto, Kazutoshi Takahashi, Isao Asaka, Yasuhiro Yamada, Yoshifumi Ubara, Eri Muso, Atsushi Fukatsu, Akira Watanabe, Yasunori Sato, Tatsutoshi Nakahata, Yasuo Mori, Akio Koizumi, Kazuwa Nakao, Shinya Yamanaka, Kenji Osafune
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
Thomas F. Allison, Peter W. Andrews, Yishai Avior, Ivana Barbaric, Nissim Benvenisty, Christoph Bock, Jennifer Brehm, Oliver Bruestle, Ivan Damjanov, Andrew Elefanty, Daniel Felkner, Paul J. Gokhale, Florian Halbritter, Lyn E. Healy, Tim X. Hu, Barbara B. Knowles, Jeanne F. Loring, Tenneille E. Ludwig, Robyn Mayberry, Suzanne Micallef, Jameelah S. Mohamed, Franz-Josef Mueller, Christine L. Mummery, Norio Nakatsuji, Elizabeth S. Ng, Steve K. W. Oh, Orla O'Shea, Martin F. Pera, Benjamin Reubinoff, Paul Robson, Janet Rossant, Bernhard M. Schuldt, Davor Solter, Koula Sourris, Glyn Stacey, Edouard G. Stanley, Hirofumi Suemori, Kazutoshi Takahashi, Shinya Yamanaka
NATURE COMMUNICATIONS
(2018)
Article
Cell Biology
Tim A. Rand, Kenta Sutou, Koji Tanabe, Daeun Jeong, Masaki Nomura, Fumiyo Kitaoka, Emi Tomoda, Megumi Narita, Michiko Nakamura, Masahiro Nakamura, Akira Watanabe, Eric Rulifson, Shinya Yamanaka, Kazutoshi Takahashi
Review
Physiology
Peter Karagiannis, Kazutoshi Takahashi, Megumu Saito, Yoshinori Yoshida, Keisuke Okita, Akira Watanabe, Haruhisa Inoue, Jun K. Yamashita, Masaya Todani, Masato Nakagawa, Mitsujiro Osawa, Yoshimi Yashiro, Shinya Yamanaka, Kenji Osafune
PHYSIOLOGICAL REVIEWS
(2019)
Article
Cell Biology
Kazutoshi Takahashi, Daeun Jeong, Songnan Wang, Megumi Narita, Xuemei Jin, Mio Iwasaki, Samuel D. Perli, Bruce R. Conklin, Shinya Yamanaka
Article
Genetics & Heredity
Kazutoshi Takahashi, Michiko Nakamura, Chikako Okubo, Zane Kliesmete, Mari Ohnuki, Megumi Narita, Akira Watanabe, Mai Ueda, Yasuhiro Takashima, Ines Hellmann, Shinya Yamanaka
Summary: Research suggests that HERV-H type endogenous retroviruses expressed in PSCs play a crucial role in maintaining pluripotent stem cell identity. However, the study on ESRG reveals that it is dispensable for the maintenance and recapturing of human pluripotency, as it has minimal impact on the global gene expression of PSCs or their differentiation potential towards trilineage.
Article
Medicine, Research & Experimental
Rina Hashimoto, Ayaka Sakamoto, Sayaka Deguchi, Renxing Yi, Emi Sano, Akitsu Hotta, Kazutoshi Takahashi, Shinya Yamanaka, Kazuo Takayama
Summary: This study investigated the roles of neuropilin-1, basigin, TMPRSSs, and CTSs in SARS-CoV-2 infection using the CRISPR interference system and ACE2-expressing human iPS cells. Double knockdown of TMPRSS2 and CTSB significantly reduced the viral load. Combination of CTPB and TMPRSS2 inhibitors showed highly efficient antiviral effects against various SARS-CoV-2 variants, independent of gender.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Multidisciplinary Sciences
Mio Iwasaki, Yuka Kawahara, Chikako Okubo, Tatsuya Yamakawa, Michiko Nakamura, Tsuyoshi Tabata, Yohei Nishi, Megumi Narita, Akira Ohta, Hirohide Saito, Takuya Yamamoto, Masato Nakagawa, Shinya Yamanaka, Kazutoshi Takahashi
Summary: By combining transcriptome and proteome profiling, this study reveals the crucial role of posttranscriptionally regulated genes in the survival of iPSCs, especially those genes specifically necessary for iPSC survival.
Article
Multidisciplinary Sciences
Renxing Yi, Rina Hashimoto, Ayaka Sakamoto, Yasufumi Matsumura, Miki Nagao, Kazutoshi Takahashi, Kazuo Takayama
Summary: This study identified and characterized host factors essential for SARS-CoV-2 infection using iPS cells and airway organoids. EXOC2 was found to be an important host factor, and its knockdown downregulated SARS-CoV-2 infection by regulating IFNW1 expression. Controlling the expression level of EXOC2 may help prevent SARS-CoV-2 infection.
Article
Biochemical Research Methods
Akira Kunitomi, Ryoko Hirohata, Vanessa Arreola, Mitsujiro Osawa, Tomoaki M. Kato, Masaki Nomura, Jitsutaro Kawaguchi, Hiroto Hara, Kohji Kusano, Yasuhiro Takashima, Kazutoshi Takahashi, Keiichi Fukuda, Naoko Takasu, Shinya Yamanaka
Summary: This study reports a modified Sendai virus vector system that can generate transgene-free naive human iPSCs with superior differentiation potential. This method is applicable to various cell types and allows for the generation of naive iPSCs in a feeder-free culture, resulting in better differentiation compared to conventional methods.
CELL REPORTS METHODS
(2022)
Article
Biochemical Research Methods
Kazutoshi Takahashi, Chikako Okubo, Michiko Nakamura, Mio Iwasaki, Yuka Kawahara, Tsuyoshi Tabata, Yousuke Miyamoto, Knut Woltjen, Shinya Yamanaka
Summary: This study describes an improved method for subculturing human PSCs, which enhances their viability and reproducibility, while maintaining their characteristics and attenuating the growth advantage of abnormal subpopulations.
CELL REPORTS METHODS
(2022)
