Article
Pharmacology & Pharmacy
Napaphak Jaipakdee, Kanokwan Jarukamjorn, Waraporn Putalun, Ekapol Limpongsa
Summary: The study evaluated the permeation behaviors and chemical stability of miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica (PM) in transdermal gels. Results indicated that hydroalcoholic gel exhibited the best permeation of miroestrol and deoxymiroestrol, with the highest skin permeability coefficient. Long-term stability study showed that miroestrol contents remained constant while deoxymiroestrol decreased significantly after 30 days of storage, regardless of conditions.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Article
Agricultural Engineering
Dolly Rani, Khwanlada Kobtrakul, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: The study evaluated the impact of magnetized water on the growth and secondary metabolite production of Pueraria candollei var. mirifica (PM) suspension cells. The results showed that magnetized water treatment increased biomass accumulation, altered the accumulation pattern of isoflavonoids, and enhanced antioxidant activity in the cells.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemical Research Methods
Wipawee Juengsanguanpornsuk, Nut Poopanee, Supaluk Krittanai, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun, Gorawit Yusakul
Summary: Two immunoaffinity separation approaches using active fragment antigen-binding (Fab) antibodies were developed to selectively separate miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica. The methods were successful in purifying the compounds from the plant extract.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Natthapon Chuphol, Nuttikarn Nokkaew, Fonthip Makkliang, Worapol Sae-Foo, Suppalak Phaisan, Waraporn Putalun, Seiichi Sakamoto, Gorawit Yusakul
Summary: This study developed and validated an ICA with a monoclonal antibody exhibiting similar reactivity against Mi and Dmi. The ICA showed a LOD at 1 and 16 mu g/mL for Mi and Dmi, respectively. The cross-reactivity of the ICA with Dmi was lower than that observed with the icELISA, and the cross-reactivity of ICA against other compounds of the PM was correlated with those of icELISA.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biotechnology & Applied Microbiology
Dolly Rani, Visarut Buranasudja, Khwanlada Kobtrakul, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: This study investigated the effects of salicylic acid, chitosan, and methyl jasmonate on bioactive compounds in suspension cells of Pueraria candollei var. mirifica, with salicylic acid showing the most promising results. The extracts from salicylic acid-elicited cells exhibited the highest antioxidant activity and promoted fibroblast proliferation, indicating potential antiaging activities of Pueraria candollei var. mirifica extracts.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Chemistry, Applied
Gorawit Yusakul, Wipawee Juengsanguanpornsuk, Boonchoo Sritularak, Suppalak Phaisan, Thaweesak Juengwatanatrakul, Waraporn Putalun
Summary: (+)-7-O-Methylisomiroestrol (MeI), a novel chromene found in the Pueraria candollei var. mirifica (PM) root, has a higher estrogenic potency compared to isomiroestrol but lower than deoxymiroestrol and miroestrol. MeI serves as a new estrogenic biomarker for the chemical standardization of PM extract for health product development.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Fonthip Makkliang, Wipawee Juengsanguanpornsuk, Suppalak Phaisan, Attapon Sakdamas, Waraporn Putalun, Seiichi Sakamoto, Gorawit Yusakul
Summary: The study successfully enhanced the estrogenic activity of Pueraria candollei var. mirifica (PM) extract by treating it with immobilized beta-glucosidase, demonstrating potential industrial application.
Article
Biochemistry & Molecular Biology
Yaowared Chulikhit, Wichitsak Sukhano, Supawadee Daodee, Waraporn Putalun, Rakvajee Wongpradit, Charinya Khamphukdee, Kaoru Umehara, Hiroshi Noguchi, Kinzo Matsumoto, Orawan Monthakantirat
Summary: The phytoestrogen-enriched plant Pueraria mirifica extract was found to improve cognitive impairment and hippocampal oxidative stress induced by ovariectomy in mice. The treatment attenuated oxidative brain damage and dysfunctions in the hippocampal antioxidant system while preventing neuroinflammation, thereby suggesting potential benefits for cognitive deficits associated with menopause/ovariectomy.
Article
Integrative & Complementary Medicine
Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Witsarut Kraithong, Waraporn Putalun
Summary: The study focused on extracting high phytoestrogen content from Pueraria candollei var. mirifica root cortex, with the most efficient extraction achieved using 10% ethanol. It was found that potent phytoestrogens and isoflavonoids were mainly distributed in the soluble extract, while less polar unidentified compounds were concentrated in the insoluble extract. The estrogenic activity of the soluble extract was confirmed through cell proliferation assays, with deoxymiroestrol identified as the key contributor to the proliferative effects of PM.
JOURNAL OF HERBAL MEDICINE
(2021)
Article
Chemistry, Medicinal
Sayaka Masada, Junko Hosoe, Ryoko Arai, Yosuke Demizu, Takashi Hakamatsuka, Yukihiro Goda, Nahoko Uchiyama
Summary: By developing a quantitative analysis method for miroestrol in Pueraria mirifica (PM) products using kwakhurin as a reference standard, this study successfully monitored the accuracy of miroestrol content in PM products. The results showed that the developed LC/PDA/MS method with RMS was accurate for routine monitoring of miroestrol content in PM products to control their quality.
CHEMICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Chemistry, Applied
Worapol Sae-Foo, Supaluk Krittanai, Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Tharita Kitisripanya, Seiichi Sakamoto, Waraporn Putalun
Summary: A study successfully established an ELISA method for quantifying deoxymiroestrol in Pueraria candollei, demonstrating accurate detection and recovery of the original content. The developed ELISA method showed consistent results with traditional ELISA method, which can be applied for quality control of deoxymiroestrol in Pueraria candollei.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Wipawee Juengsanguanpornsuk, Gorawit Yusakul, Tharita Kitisripanya, Supaluk Krittanai, Thaweesak Juengwatanatrakul, Seiichi Sakamoto, Waraporn Putalun
Summary: The developed ELISA method is effective in detecting trace amounts of methylisomiroestrol in Pueraria candollei, which is crucial for standardization of the products and improving the quality of plant-derived products.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Engineering, Chemical
Fusong Liu, Wanru Wang, Xiaohui Feng, Fan Cao, Guilin Dai, Kewen Tang
Summary: Fractional extraction was used to separate puerarin from crude Pueraria extract, with high purity of 98.0% achieved through two-step fractional extraction. This method is effective for accurately separating active ingredients in Chinese herbal medicine on a commercial scale.
CHEMICAL ENGINEERING SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Dolly Rani, Khwanlada Kobtrakul, Jittima Amie Luckanagul, Wuttinont Thaweesest, Pornchai Rojsitthisak, Wanchai De-Eknamkul, Sornkanok Vimolmangkang
Summary: The age of Pueraria candollei var. mirifica callus significantly affects its chemical profiles and bioactivities, with different growth stages exhibiting varied gene expression and chemical compositions. This study provides important data for further research on the biosynthetic pathway of isoflavonoids.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Environmental Sciences
Nattinee Jantaratnotai, Anusorn Thampithak, Pongsak Utaisincharoen, Darawan Pinthong, Pimtip Sanvarinda
Summary: The ethyl acetate extract of Pueraria mirifica exhibits anti-inflammatory effects on microglial activation, reducing the production of inflammatory substances from activated microglia. This could potentially be beneficial in relieving the severity of neurological disorders.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Chemistry, Applied
Jiranan Chaingam, Rattanathorn Choonong, Thaweesak Juengwatanatrakul, Tripetch Kanchanapoom, Waraporn Putalun, Gorawit Yusakul
Summary: The anti-inflammatory effects of root and callus cultures of Eurycoma longifolia Jack (EL) and Eurycoma harmandiana Pierre (EH) on inflammatory cells were evaluated in this study. The tissue cultures produced compounds that exhibited anti-inflammatory effects, suggesting they could be a valuable and sustainable source of active ingredients.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Kantapich Kongpol, Namfa Sermkaew, Fonthip Makkliang, Sirinan Khongphan, Litavadee Chuaboon, Attapon Sakdamas, Seiichi Sakamoto, Waraporn Putalun, Gorawit Yusakul
Summary: This study presents the design of hydrophobic deep eutectic solvents (HDESs) and HDES-based microemulsions for the extraction of curcuminoids and ar-turmerone from Curcuma longa L. The results show that HDES and its microemulsions are more effective and environmentally friendly than conventional organic solvents, and they are also compatible with food and pharmaceutical formulations.
Article
Plant Sciences
Rattanathorn Choonong, Sasiporn Ruangdachsuwan, Theeraporn Churod, Somnuek Palabodeewat, Surat Punyahathaikul, Sanjira Juntarapornchai, Kunjimas Ketsuwan, Jukrapun Komaikul, Promsin Masrinoul, Tharita Kitisripanya, Thaweesak Juengwatanatrakul, Gorawit Yusakul, Tripetch Kanchanapoom, Waraporn Putalun
Summary: This study investigated the antiviral activity of Eurycoma longifolia and Eurycoma harmandiana and found that their quassinoid constituents have potential therapeutic effects against coronaviruses. These findings provide new insights for the treatment of COVID-19.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Worapol Sae-Foo, Gorawit Yusakul, Tharita Kitisripanya, Poomraphie Nuntawong, Seiichi Sakamoto, Waraporn Putalun
Summary: In this study, recombinant Fab antibodies against miroestrol were developed and applied for the quality control of Pueraria candollei. The Fab antibodies showed higher stability and better detection ability compared to ScFv antibodies.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Natthapon Chuphol, Nuttikarn Nokkaew, Fonthip Makkliang, Worapol Sae-Foo, Suppalak Phaisan, Waraporn Putalun, Seiichi Sakamoto, Gorawit Yusakul
Summary: This study developed and validated an ICA with a monoclonal antibody exhibiting similar reactivity against Mi and Dmi. The ICA showed a LOD at 1 and 16 mu g/mL for Mi and Dmi, respectively. The cross-reactivity of the ICA with Dmi was lower than that observed with the icELISA, and the cross-reactivity of ICA against other compounds of the PM was correlated with those of icELISA.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Wipawee Juengsanguanpornsuk, Nut Poopanee, Supaluk Krittanai, Seiichi Sakamoto, Hiroyuki Tanaka, Waraporn Putalun, Gorawit Yusakul
Summary: Two immunoaffinity separation approaches using active fragment antigen-binding (Fab) antibodies were developed to selectively separate miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica. The methods were successful in purifying the compounds from the plant extract.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Gorawit Yusakul, Chongdee Thammakhet-Buranachai, Sujittra Poorahong, Seiichi Sakamoto, Fonthip Makkliang
Summary: An eco-friendly and reusable DESs-MCC/PVA sponge was synthesized and showed good efficiency and reproducibility in the extraction of parabens in milk, beverages, and tea samples. The sponge retained > 80% extraction efficiency and could be reused up to five times. This method offers a simple and environmentally friendly approach for the extraction of trace organic substances.
MICROCHEMICAL JOURNAL
(2023)
Correction
Chemistry, Multidisciplinary
Attapon Sakdamas, Fonthip Makkliang, Waraporn Putalun, Thaweesak Juengwatanatrakul, Tripetch Kanchanapoom, Seiichi Sakamoto, Gorawit Yusakul
Article
Chemistry, Multidisciplinary
Attapon Sakdamas, Fonthip Makliang, Waraporn Putalun, Thaweesak Juengwatanatrakul, Tripetch Kanchanapoom, Seiichi Sakamoto, Gorawit Yusakul
Summary: Micellar liquid chromatography (MLC) was used to analyze low concentration canthin-6-one alkaloids in extracts of Eurycoma longifolia Jack (EL) and Eurycoma harmandiana Pierre (EH). The MLC method showed comparable sensitivity, precision, accuracy, and acetonitrile consumption to the conventional high-performance liquid chromatography (HPLC) method. However, the HPLC method exhibited better performance for samples with low analyte concentrations and varying sample matrices, especially EL and EH samples. Overall, the MLC analysis of canthin-6-one alkaloids was limited to trace analytes due to interference by the matrix.
Article
Agricultural Engineering
Rattanathorn Choonong, Supaluk Krittanai, Khunkhang Butdapheng, Thaweesak Juengwatanatrakul, Gorawit Yusakul, Tripetch Kanchanapoom, Waraporn Putalun
Summary: The study investigated the growth kinetics of adventitious root culture of E. harmandiana and the effect of various elicitors and precursors on the contents of bioactive compounds. It was found that the elicitors and precursor significantly increased the accumulation of quassinoids and canthin-6-ones, leading to stronger anti-inflammatory effects.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Meeting Abstract
Plant Sciences
W. Sae-Foo, N. Nualkaew, G. Yusakul, W. Putalun
Meeting Abstract
Plant Sciences
S. Krittanai, W. Putalun
