Article
Chemistry, Medicinal
Ankita Singh, Afruja Khan, Tamal Ghosh, Samiran Mondal, Amirul Mallick
Summary: Acute diarrheal illness and gastroenteritis caused by Campylobacter jejuni infection are significant public health risks, especially in children under five in developing countries. Limited genetic diversity among Campylobacter jejuni and understanding of host immunity are obstacles to developing effective measures to control infection. Utilizing bacterial outer-membrane vesicles as vaccine targets shows promise in inducing specific immune responses against Campylobacter jejuni.
ACS INFECTIOUS DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Chen Gao, Yin Chen, Xin Cheng, Yi Zhang, Yueyue Zhang, Ying Wang, Zhiyuan Cui, Yaling Liao, Ping Luo, Weihui Wu, Cheng Wang, Hao Zeng, Quanming Zou, Jiang Gu
Summary: This study identified a novel immunogenic epitope from the outer membrane protein PilY1 of Pseudomonas aeruginosa. A macrophage membrane-coated nanoparticle vaccine carrying this epitope induced an immune response and conferred protection in mice.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Microbiology
Hongqiang Lou, Xusheng Li, Xiusheng Sheng, Shuiqin Fang, Shaoye Wan, Aihua Sun, Haohao Chen
Summary: In this study, a tandem epitope composed of three epitopes was developed for Campylobacter jejuni. The tandem epitope showed good stability and antigenicity, and immunization with it induced protective immunity in mice. The colonization of C. jejuni was significantly reduced, suggesting that the tandem epitope could be a candidate antigen for a genetic engineering vaccine against C. jejuni MAP.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Afruja Khan, Avijit Sardar, Pradip K. Tarafdar, Amirul I. Mallick
Summary: Campylobacter jejuni uses naturally secreted outer membrane vesicles (OMVs) to deliver toxins to target cells, relying on their biophysical and compositional attributes. The study reveals that OMVs uptake is mediated by cytosolic and multiple endocytic processes, showing target cell-specific preferential uptake.
ACS INFECTIOUS DISEASES
(2023)
Article
Microbiology
Harald Nothaft, Maria Elisa Perez-Munoz, Tianfu Yang, Abarna V. M. Murugan, Michelle Miller, Daniel Kolarich, Graham S. Plastow, Jens Walter, Christine M. Szymanski
Summary: The study shows that Campylobacter jejuni spreads through contaminated poultry products, and an attenuated Escherichia coli live vaccine strain expressing the C. jejuni N-glycan can reduce the Campylobacter load in poultry, with distinct responder and non-responder outcomes. Research indicates that antibodies from responder birds have higher Campylobacter-opsonophagocytic activity, and transplantation of cecal microbiota from responder birds can enhance vaccine-induced antigen-specific IgY responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Cheng-dong Jin, Mo-fei Li
Summary: The study demonstrates that Omp1Et is a surface-located virulence factor of E. tarda, and recombinant Omp1Et can effectively induce immune protection as a subunit vaccine.
Article
Immunology
Prerna Vohra, Cosmin Chintoan-Uta, Abi Bremner, Marta Mauri, Vanessa S. Terra, Jon Cuccui, Brendan W. Wren, Lonneke Vervelde, Mark P. Stevens
Summary: Campylobacter jejuni is a common bacterial cause of human gastroenteritis, primarily transmitted through contaminated poultry meat. Previous studies have shown that glycoconjugate vaccines with C. jejuni N-glycan provide partial protection in chickens, but subunit vaccines with FlpA or SodB proteins coupled with C. jejuni N-glycans did not show significant protection. A new vaccine strategy using glycoprotein G-ExoA with multiple C. jejuni N-glycans was evaluated in chickens, but did not result in reduced colonization by C. jejuni. The study suggests that protection against C. jejuni may be influenced by the amount of glycan presented and other study-specific variables.
Article
Microbiology
Hengchun Cao, Hanxiao Xu, Chunhui Ning, Li Xiang, Qiufang Ren, Tiantian Zhang, Yusen Zhang, Rui Gao
Summary: This study investigated the pangenome of Campylobacter jejuni, analyzing its virulence genes and potential core vaccine targets. Through experiments and analysis, five core vaccine targets with high antigenicity were identified, which play important roles in the virulence of C. jejuni in different hosts. The findings contribute to the discovery of new vaccine targets and provide a method to prevent multidrug-resistant C. jejuni.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Xiaofang Wu, Lei Ji, Yuehua Shen, Liping Chen, Deshun Xu, Fenfen Dong
Summary: Thirteen pathogenic Campylobacter strains were isolated from diarrhea cases and raw poultry meat products in Huzhou, including 11 Campylobacter jejuni and 2 Campylobacter coli strains. These strains contain multiple virulence-related genes and show high resistance to nalidixic acid, ciprofloxacin, and tetracycline. Genomic sequencing revealed the presence of tet(O) and blaOXA resistance genes in all C. jejuni strains and multiple drug resistance genes in 2 C. coli strains.
Review
Chemistry, Applied
Murat Gurbuz, Burcu Irem Omurtag Korkmaz
Summary: Poultry is a rapidly growing industry, but concerns about food safety and the presence of bacteria like Campylobacter in poultry remain. The discovery of new antimicrobials, such as eugenol, is important for ensuring the safety of poultry meat. However, further research is needed to determine the specific applications and effectiveness of eugenol as a food preservative in poultry meat safety.
Article
Biochemistry & Molecular Biology
Augustin Tshibaka Kabongo, Rajib Acharjee, Takaya Sakura, Gloria Mavinga Bundutidi, Endah Dwi Hartuti, Cadi Davies, Ozan Gundogdu, Kiyoshi Kita, Tomoo Shiba, Daniel Ken Inaoka
Summary: Campylobacter jejuni infection is a serious global health threat, and the increasing incidence and antibiotic resistance have called for the development of new drugs targeting this bacterial infection. Malate:quinone oxidoreductase (MQO) is an attractive drug target as it is essential for the survival of several bacteria and parasites but not conserved in mammals. In this study, the researchers purified and analyzed MQO from C. jejuni, showing that it can be inhibited by ferulenol and embelin, which also inhibit the growth of C. jejuni in vitro.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Benjamin Duque, Sandrine Reze, Albert Rossero, Jeanne-Marie Membre, Sandrine Guillou, Nabila Haddad
Summary: Broiler meat is considered a significant source of Campylobacter jejuni, a foodborne pathogen. Stress conditions during slaughtering can affect bacterial adaptation and resistance, leading to changes in gene expression. RT-qPCR was used to measure gene expression in 44 C. jejuni genes after thermal stresses, revealing differential expression in 26 genes. The study provides insights into the molecular mechanisms of bacterial stress response in real-life processing conditions.
Article
Food Science & Technology
Ana Rita Barata, Barbara Nunes, Ricardo Oliveira, Hugo Guedes, Carina Almeida, Maria Jose Saavedra, Gabriela Jorge da Silva, Goncalo Almeida
Summary: The growing belief in the health benefits of natural products, such as raw milk, has led to behaviors that can contribute to food safety issues and increased healthcare costs. This study investigated the occurrence and seasonality of Campylobacter spp. in raw cow's milk and the potential routes of contamination in dairy farm environments. The findings highlight the potential risk of campylobacteriosis associated with consuming raw milk and emphasize the importance of routine surveillance in dairy farms for zoonotic pathogens.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Fisheries
Mo-fei Li, Yu-ting Du, Cheng-dong Jin, Xue-peng Li, Yuan-yuan Sun
Summary: The study found that Ail1 protein in Edwardsiella piscicida plays a crucial role in its pathogenicity, affecting the survival and infection abilities of the bacteria as well as its virulence in hosts. This suggests that Ail1 may be explored as a potential subunit vaccine in aquaculture.
Article
Biochemistry & Molecular Biology
Dung T. Huynh, Wouter S. P. Jong, Gregory M. Koningstein, Peter van Ulsen, Joen Luirink
Summary: Ct-MOMP, a promising subunit-based vaccine candidate, is difficult to express in its native structure in E. coli outer membrane. Co-expression of the Bam complex improved the expression and localization of recombinant Ct-MOMP, leading to correct folding and assembly into a beta-barrel conformation at the cell surface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Milton Thomas, Zhao Wang, Chithra C. Sreenivasan, Ben M. Hause, Gourapura J. Renukaradhya, Feng Li, David H. Francis, Radhey S. Kaushik, Mahesh Khatri
Review
Immunology
Gourapura J. Renukaradhya, Xiang-Jin Meng, Jay G. Calvert, Michael Roof, Kelly M. Lager
Review
Immunology
Gourapura J. Renukaradhya, Xiang-Jin Meng, Jay G. Calvert, Michael Roof, Kelly M. Lager
Article
Virology
Basavaraj Binjawadagi, Yashavanth Shaan Lakshmanappa, Zhu Longchao, Santosh Dhakal, Jagadish Hiremath, Kang Ouyang, Duan-Liang Shyu, Jesus Arcos, Shang Pengcheng, Aaron Gilbertie, Federico Zuckermann, Jordi B. Torrelles, Daral Jackwood, Ying Fang, Gourapura J. Renukaradhya
ARCHIVES OF VIROLOGY
(2016)
Article
Virology
Yanhua Li, Duan-Liang Shyu, Pengcheng Shang, Jianfa Bai, Kang Ouyang, Santosh Dhakal, Jagadish Hiremath, Basavaraj Binjawadagi, Gourapura J. Renukaradhya, Ying Fang
JOURNAL OF VIROLOGY
(2016)
Article
Microbiology
Varun Dwivedi, Cordelia Manickam, Santosh Dhakal, Basavaraj Binjawadagi, Kang Ouyang, Jagadish Hiremath, Mahesh Khatri, Jacquelyn Gervay Hague, Chang Won Lee, Gourapura J. Renukaradhya
VETERINARY MICROBIOLOGY
(2016)
Review
Agriculture, Dairy & Animal Science
Joan K. Lunney, Ying Fang, Andrea Ladinig, Nanhua Chen, Yanhua Li, Bob Rowland, Gourapura J. Renukaradhya
ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 4
(2016)
Article
Immunology
Jennifer C. Bailey, Abhirami K. Iyer, Gourapura J. Renukaradhya, Yinling Lin, Hoa Nguyen, Randy R. Brutkiewicz
Article
Nanoscience & Nanotechnology
Basavaraj Binjawadagi, Varun Dwivedi, Cordelia Manickam, Kang Ouyang, Jordi B. Torrelles, Gourapura J. Renukaradhya
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2014)
Article
Nanoscience & Nanotechnology
Basavaraj Binjawadagi, Varun Dwivedi, Cordelia Manickam, Kang Ouyang, Yun Wu, Ly James Lee, Jordi B. Torrelles, Gourapura J. Renukaradhya
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2014)
Article
Immunology
Mohamed Elaish, Ming Xia, John M. Ngunjiri, Amir Ghorbani, Hyesun Jang, K. C. Mahesh, Michael C. Abundo, Santosh Dhakal, Renukaradhya Gourapura, Xi Jiang, Chang-Won Lee
Article
Nanoscience & Nanotechnology
Sankar Renu, Ashley D. Markazi, Santosh Dhakal, Yashavanth S. Lakshmanappa, Revathi Shanmugasundaram, Ramesh K. Selvaraj, Gourapura J. Renukaradhya
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2020)
Review
Veterinary Sciences
Santosh Dhakal, Gourapura J. Renukaradhya
VETERINARY RESEARCH
(2019)
Article
Veterinary Sciences
Kang Ouyang, Jagadish Hiremath, Basavaraj Binjawadagi, Duan-Liang Shyu, Santosh Dhakal, Jesus Arcos, Rose Schleappi, Lynette Holman, Michael Roof, Jordi B. Torrelles, Gourapura J. Renukaradhya
VETERINARY RESEARCH
(2016)
Article
Veterinary Sciences
Kang Ouyang, Duan-Liang Shyu, Santosh Dhakal, Jagadish Hiremath, Basavaraj Binjawadagi, Yashavanth S. Lakshmanappa, Rui Guo, Russell Ransburgh, Kathryn M. Bondra, Phillip Gauger, Jianqiang Zhang, Terry Specht, Aaron Gilbertie, William Minton, Ying Fang, Gourapura J. Renukaradhya
VETERINARY RESEARCH
(2015)
Article
Agriculture, Dairy & Animal Science
Jesper Tessin, Judith Rohde, Arne Jung, Nicole Kemper, Jochen Schulz
Summary: In this study, samples from poultry house environment and culled birds were collected, and E. cecorum was successfully isolated. The principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate transmission routes and optimize biosecurity measures.
Article
Agriculture, Dairy & Animal Science
Junqin Zhang, Zimin Xie, Yanlin Pan, Zuoxin Chen, Yunzhen Huang, Linlin Li, Jiawen Dong, Yong Xiang, Qi Zhai, Minhua Sun, Xingying Li, Shujian Huang, Ming Liao
Summary: In recent years, there has been an increase in the occurrence of fowl adenovirus 2 (FAdV-2) in China, posing a significant threat to the poultry industry. This study investigated the epidemiology, phylogenetic relationship, genomic characteristics, and pathogenicity of FAdV-2. The results showed that FAdV-2 was the most commonly detected serotype among various FAdV serotypes. Genetic analysis revealed that these FAdV-2 strains formed a distinct branch within the FAdV-D group and had a high nucleotide similarity with reference strains. Recombination analysis identified recombination events between FAdV-2 and FAdV-11 in some strains. Pathogenicity experiments demonstrated that a recombinant FAdV-2 strain caused a high mortality rate and more severe disease in chickens. These findings contribute to our understanding of FAdV-2 and provide a basis for vaccine development.
Article
Agriculture, Dairy & Animal Science
Ebtsam Iraqi, Ali Abdel Hady, Nadia Elsayed, Hanaa Khalil, Amina El-Saadany, Karim El-Sabrout
Summary: Thermal manipulation during egg incubation, particularly at 12 to 18 days, has positive effects on embryonic development, hatching characteristics, chick quality, as well as posthatch thermotolerance and performance.
Article
Agriculture, Dairy & Animal Science
Yong Liu, Mingxin Song, He Bai, Chunhua Wang, Fei Wang, Qi Yuan
Summary: Dietary curcumin supplementation can significantly improve egg quality of quails in the late laying period, primarily by improving lipid metabolism and selectively regulating the intestinal microbial community.
Article
Agriculture, Dairy & Animal Science
Lei Zhang, Zhixuan Song, Peiyao Li, Xingju Song, Xinming Tang, Dandan Hu
Summary: This study evaluated the vaccine potential of recombinant proteins for avian coccidiosis caused by Eimeria. The results showed that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.
Article
Agriculture, Dairy & Animal Science
Milan Kumar Sharma, Amit Kumar Singh, Doyun Goo, Venkata Sesha Reddy Choppa, Hanseo Ko, Hanyi Shi, Woo Kyun Kim
Summary: The study investigates the effect of Eimeria infection on gastrointestinal physiology, growth performance, and egg production of Hy-Line W-36 laying hens during peak production. Results show that Eimeria infection negatively affects body weight, feed intake, immune response, and nutrient transporter gene expression. Egg production temporarily ceases in hens infected with high and medium-high dosages of Eimeria.
Article
Agriculture, Dairy & Animal Science
Elwy A. Ashour, Mahmoud Kamal, Hayman A. A. Altaie, Ayman A. Swelum, Gamaleldin M. Suliman, Guillermo Tellez-Isaias, Mohamed E. Abd El-Hack
Summary: This study evaluated the effects of CP and ME on the performance and nutritional value of Japanese quail lay chicken. The results showed that protein and energy levels significantly influenced all productive outcomes except feed conversion ratio. The combination of dietary energy and protein levels had a substantial effect on all egg quality trials evaluated. It is recommended to feed layer Japanese quail between the ages of 8 and 20 weeks during the summer with a dietary energy content of 2,900 kcal ME/kg and 20% CP.
Article
Agriculture, Dairy & Animal Science
J. E. Doornweerd, R. F. Veerkamp, B. de Klerk, M. van der Sluis, A. C. Bouwman, E. D. Ellen, G. Kootstra
Summary: Tracking group-housed individual broilers using video can provide valuable information on their health, welfare, and performance. This study conducted a comprehensive analysis on broiler tracking on video and potential tracking errors, highlighting the potential and challenges of phenotyping broiler locomotion. The results emphasized the importance of addressing tracking errors, potential algorithm improvements, and the need for an external animal identification system for efficient locomotion phenotyping.
Article
Agriculture, Dairy & Animal Science
Jiayu Wu, Yanan Wang, Yu An, Changyu Tian, Lingfeng Wang, Zuhong Liu, Desheng Qi
Summary: This study compared the gene expression differences in liver tissues of laying hens at different ages using RNA sequencing, and found that the transport and metabolism of amino acids changed during the growing period, which influenced the growth and development of the hens.
Article
Agriculture, Dairy & Animal Science
J. Raabe, G. Raveendran, W. Otten, K. Homeyer, T. Bartels
Summary: This study investigated the effects of different flicker frequencies on the behavior, performance, and stress response in male turkeys. The results showed that flicker frequencies of 165 Hz or higher had no detrimental effect on growth performance, injurious pecking, or endocrine stress response in the turkeys.
Article
Agriculture, Dairy & Animal Science
Adamu Mani Isa, Yanyan Sun, Yuanmei Wang, Yunlei Li, Jingwei Yuan, Aixin Ni, Hui Ma, Lei Shi, Hailai Hagos Tesfay, Yunhe Zong, Panlin Wang, Pingzhuang Ge, Jilan Chen
Summary: This study sequenced mRNAs and lncRNAs in the ovary stroma of specific chicken breeds and their reciprocal crossbreds to understand the molecular mechanism of heterosis for egg production. Results showed that genes with nonadditive expression were related to follicle growth and atresia as well as oxidative stress in the crossbred hens.
Article
Agriculture, Dairy & Animal Science
Zhepeng Wang, Qiu Chen, Yiwei Wang, Yulu Wang, Ruifang Liu
Summary: The study investigates the functional variants associated with chicken eggshell color in the SLCO1B3 gene by measuring the eggshell color of Lueyang black-boned chickens and analyzing the genetic data. It identifies 15 variants in SLCO1B3 that are significantly associated with eggshell color. However, the functional analysis shows that one specific variant, 1B3_SNP108, does not have a significant effect on promoter activity. This study highlights the importance of SLCO1B3 and its variants in breeding eggshell color.
Article
Agriculture, Dairy & Animal Science
P. Yin, Q. Tong, B. M. Li, W. C. Zheng, Y. Wang, H. Q. Peng, X. L. Xue, S. Q. Wei
Summary: This study investigated the welfare and health of laying hens in a multitier system. The results showed that the spatial distribution of hens changed with increasing age, and lower-ranking hens preferred to stay in lower tiers to avoid competition but still had access to resources. This improved the welfare and health of the hens near the end of the laying cycle.
Article
Agriculture, Dairy & Animal Science
Wenwen Liang, Siqiong Jie, Yiqin Wang, Xiaowei Wen, Yanling Xiong, Xiaoxi Lin, Zhengrong Lv
Summary: This study examined the effect of risk perceptions on preventive behavior among vendors in the live poultry wholesale market and the live poultry retail market in Guangdong Province using the health belief model and structural equation modeling. The results showed that perceived severity and perceived benefits positively influenced vendors' self-efficacy in the wholesale market, while perceived barriers negatively affected self-efficacy in both the wholesale and retail markets. Moreover, cues to action mediated the relationship between perceived severity, perceived benefits, and self-efficacy, and also mediated the effect of perceived susceptibility among wholesale market vendors.
Article
Agriculture, Dairy & Animal Science
Shreeya Sharma, Raveendra R. Kulkarni, Shayan Sharif, Hosni Hassan, Mohammadali Alizadeh, Scott Pratt, Khaled Abdelaziz
Summary: The study investigates the impact of exposing chick embryos to antigens via in ovo delivery of poultry-specific lactobacilli on the expression of genes associated with early bursal development and maturation. Results indicate that the supplementation of poultry-specific lactobacilli to chick embryos can accelerate the development and immunological maturation of the bursa of Fabricius.