Article
Engineering, Environmental
Jiacong Wu, Xinnan Ma, Chunmei Li, Xiangtong Zhou, Juan Han, Lei Wang, Hongjun Dong, Yun Wang
Summary: A novel photon-enzyme cascade catalysis system, composed of hybrid HRP-CN/Cu-3(PO4)(2) nanoflowers, was successfully constructed. This system exhibits excellent immobilized enzyme enzymatic properties and better tolerance to extreme conditions compared to free HRP. It shows outstanding degradation performance for bisphenol A (BPA) and can replace traditional systems for the harmless treatment of BPA in wastewater.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Silvina V. Kergaravat, Silvia N. Fabiano, Adriana R. Soutullo, Silvia R. Hernandez
Summary: Glyphosate is the most widely used herbicide in Argentina, and there is controversy surrounding its safety. Two electrochemical screening methods based on horseradish peroxidase (HRP) inhibition have been developed for glyphosate detection.
MICROCHEMICAL JOURNAL
(2021)
Article
Engineering, Chemical
Jiacong Wu, Xinnan Ma, Ting He, Juan Han, Yan Zhu, Chunmei Li, Yun Wang
Summary: The structural limitation of the photocatalyst itself hinders the increase in enzyme loading amount in the photo-enzyme coupling system. A g-C3N4-based photocatalyst ACN(45) with high enzyme loading amount was synthesized to address this issue. The channels in ACN(45) matched well with the size of the enzyme molecules, enabling a loading amount 6.3 times higher than pure g-C3N4. The ACN(45) channels also improved enzyme activity and stability, and reduced enzyme leaching. The high loading amount of the photo-enzyme coupling system enhanced the photocatalytic degradation of BPA, achieving a degradation rate 5.9 times higher than pure g-C3N4 within 1 hour. These findings provide a simple approach to design a photo-enzyme coupling system with high enzyme loading and stability.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jakub Zdarta, Oliwia Degorska, Katarzyna Jankowska, Agnieszka Rybarczyk, Adam Piasecki, Filip Ciesielczyk, Teofil Jesionowski
Summary: Immobilization of horseradish peroxidase in sodium alginate capsules and further trapping in poly(vinyl chloride) electrospun fibers enhances enzyme stability, reusability, catalytic properties, and substrate affinity, making it a promising tool for enzymatic conversion of pharmaceutically active ingredients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Guangming Li, Hao Liu, Tianding Hu, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: This study reports a dimensionality engineering strategy to convert conventional 2D Fe-N-4 centers into 3D structures by integrating oxidized sulfur functionalities onto the carbon plane. The results suggest that oxidized sulfur functionalities can serve as binding sites for assisting substrate orientation and facilitating the desorption of H2O, resulting in an outstanding specific activity of up to 119.77 U mg(-1), which is 6.8 times higher than that of conventional FeN4C SAzymes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Environmental Sciences
Jing-Jing Liu, Jong-Gook Kim, Hye-Bin Kim, Shakya Abeysinghe, Ying-Wu Lin, Kitae Baek
Summary: Enzyme-based biocatalytic treatment is an effective method for degrading organic pollutants. In this study, horseradish peroxidase (HRP) was immobilized on functionalized rice straw biochar using glutaraldehyde as a crosslinker. The biochar was pretreated by electrochemical and acid treatments to enhance its immobilizing ability.
Article
Nanoscience & Nanotechnology
Gema Vivo-Llorca, Angela Morella-Aucejo, Alba Garcia-Fernandez, Paula Diez, Antoni Llopis-Lorente, Mar Orzaez, Ramon Martinez-Manez
Summary: In this study, a novel nanodevice based on enzyme-functionalized gold nanoparticles for enzyme prodrug therapy (EPT) in breast cancer was designed and evaluated. The results showed that the nanodevice was effectively internalized by breast cancer cells and co-treatment with the nanodevice and enzyme prodrug significantly reduced the viability of breast cancer cells. The findings from three-dimensional tumor models also demonstrated the therapeutic effectiveness of this nanodevice.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Physical
Xiaonan Li, Xirong Huang
Summary: In this study, a functionalized organic salt was used as electrolyte and buffer to construct a H2O/AOT/isooctane bicontinuous microemulsion for enzymatic synthesis of polyaniline. The functionalized organic salt was found to have the ability to tune the phase inversion temperature of the microemulsion system. The stability and conductivity of the microemulsion were found to be better than those of the control experiments formulated with other surfactants and inorganic salt.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Medicine, Research & Experimental
Diana Humer, Valentina Furlanetto, Anna-Katharina Schruef, Angelika Wlodarczyk, Mario Kuttke, Christina Divne, Oliver Spadiut
Summary: Traditional cancer treatment relies on chemotherapy and radiation therapy, while targeted therapy shows promise with the need to address the immunogenicity issue of plant-derived enzymes. Research on unglycosylated HRP variants demonstrates the potential of targeting cancer cells.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Environmental Sciences
Cyntia Ely, Diego Hoefling Souza, Mylena Fernandes, Viviane Trevisan, Everton Skoronski
Summary: This study evaluated the bioconversion of phenols in biorefinery wastewater using Horseradish peroxidase (HRP) and the Fenton process. The enzymatic treatment achieved a phenol conversion rate of 97.5%, while the Fenton process was used as a complementary method for further phenol removal. The combination of HRP and Fenton proved to be effective in treating biorefinery wastewater and removing phenols.
ENVIRONMENTAL TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hongfei Ni, Yiwei Zhang, Chuhong Zong, Zhengbo Hou, Hang Song, Yong Chen, Xuesong Liu, Tengfei Xu, Yingjie Luo
Summary: In this study, new tropine-based functionalized acidic ionic liquids (FAILs) were synthesized and demonstrated excellent catalytic performance in the synthesis of aspirin. The ionic liquid [Trps][OTs] exhibited high yield and selectivity, proving its potential as an ideal green solvent for esterification reactions and a suitable substitute for traditional catalysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Friederike Dehli, Cosima Stubenrauch, Alexander Southan
Summary: This study demonstrates the potential of biotinylated gelatin methacryloyl in the preparation of hydrogel foams and the successful control of substrate conversion rate by altering the hydrogel morphology.
MACROMOLECULAR BIOSCIENCE
(2022)
Review
Chemistry, Physical
Vishal Kandathil, Siddappa A. Patil
Summary: Nanozymes, with their intrinsic enzyme-like activity, have gained significant attention in the scientific community worldwide. The development of Single-Atom Nanozymes (SANs) presents numerous opportunities for real-time applications in various fields. By evenly distributing active sites on a solid support, SANs exhibit high efficiency, activity, and sustainability, similar to homogeneous catalysts.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2021)
Article
Engineering, Environmental
Shanshan Zhang, Dongliang Liu
Summary: We developed a two-step method for constructing a stable enzyme@MOF biomimetic membrane, which can efficiently remove micropollutants from water. The enzyme@MOF composite membrane integrates MOF adsorption, enzyme catalysis, and membrane separation functions, and exhibits outstanding stability and high efficiency. This new method provides a simple and fast approach to improve water purification.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Chemistry, Applied
Yishun Yao, Bo Zhang, Huiyun Pang, Yequn Wang, Hongfei Fu, Xiangwei Chen, Yunyang Wang
Summary: This paper investigates the effects of radio frequency (RF) heating on the activity and structure of horseradish peroxidase (HRP). HRP was heated to different temperatures (50, 70, and 90 degrees C) with varying electrode gaps (100, 110, and 120 mm). The results show that RF heating at 120 mm electrode gap leads to more severe enzyme inactivation and conformational destruction, indicated by changes in Soret band, secondary structure content, and tryptophan fluorescence intensity.
Article
Cell Biology
Yessica Zamudio-Cuevas, Marco A. Andonegui-Elguera, Ariadna Aparicio-Juarez, Edson Aguillon-Solis, Karina Martinez-Flores, Erika Ruvalcaba-Paredes, Cristina Velasquillo-Martinez, Clemente Ibarra, Valentin Martinez-Lopez, Marwin Gutierrez, Roeb Garcia-Arrazola, Carmen G. Hernandez-Valencia, Alejandra Romero-Montero, Miguel A. Hernandez-Valdepena, Miquel Gimeno, Roberto Sanchez-Sanchez
Summary: Studies have shown that PGAL can reduce the release of pro-inflammatory cytokines, decrease cell proliferation and adhesion, potentially offering therapeutic benefits for certain inflammatory diseases.
Article
Materials Science, Biomaterials
Miguel A. Hernandez-Valdepena, Carmen G. Hernandez-Valencia, Pablo Labra-Vazquez, Carmen Wacher, Gloria Diaz-Ruiz, Alfredo Vazquez, Jose Pedraza-Chaverri, Keiko Shirai, Alberto Rosas-Aburto, Eduardo Vivaldo-Lima, Eduardo Barzana, Romina Rodriguez-Sonoja, Miquel Gimeno
Summary: Microwave-mediated grafting of L-Arginine onto naturally derived and stable multiradical poly(gallic acid) has been achieved successfully. This polymeric material showed no adverse effects on human cells and preserved its antioxidant properties, while also exhibiting antimicrobial activity against various bacterial strains. The mechanism of bacterial inhibition was related to the formation of porous channels on the membrane, influenced by the helical secondary structure of the polymer and the amino acid guanidyl moieties interacting with bacterial membranes.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Energy & Fuels
Armando Navarro, Carmina Montiel, Jesus Gracia-Fadrique, Alberto Tecante, Eduardo Barzana
Summary: Supercritical carbon dioxide (SCCO2) pretreatment of blue agave bagasse (BAB) enhances enzymatic hydrolysis (EH) to simple sugars for bioethanol fermentation.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Engineering, Chemical
Juan Carlos Morales-Huerta, Oscar Hernandez-Melendez, Martin Guillermo Hernandez-Luna, Octavio Manero, Eduardo Barzana, Eduardo Vivaldo-Lima
Summary: The study used a mathematical model to analyze the de-construction and degradation of blue agave bagasse in a laboratory-scale extruder. Experimental results showed that NaOH concentration, residence time, and temperature play key roles in the extraction of cellulose and hemicelluloses. The model demonstrated good agreement with experimental data, suggesting its potential for industrial process analysis and scaling up in the future.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Cell Biology
Yessica Zamudio-Cuevas, Valentin Martinez-Lopez, Ivan Alejandro Lujan-Juarez, Nathalie Montano-Armendariz, Karina Martinez-Flores, Javier Fernandez-Torres, Miquel Gimeno, Roberto Sanchez-Sanchez
Summary: Gout is a chronic and degenerative disease caused by the crystalline deposit of monosodium urate, and poly-gallic acid has been found to be effective in reducing MSU-induced inflammation and oxidative stress.
Article
Cell Biology
Claudia Denisse Mendoza-Viveros, Oscar Gutierrez-Perez, Maria Josefa Bernad-Bernad, Luis Medina-Torres, Minerva Monroy-Barreto, Miquel Gimeno, Maria Elena Trujillo-Ortega
Summary: The use of liposomes with trehalose appears to be a promising option for boar semen cryopreservation, with a marked effect on rheological properties. The proposed HPLC-ELSD method was effective for measuring trehalose in cryopreserved cell samples.
Article
Biotechnology & Applied Microbiology
Angel Espinoza-Gonzalez, Carmen Hernandez-Valencia, Luis Cedeno-Caero, Roberto Sanchez-Sanchez, Carmina Montiel, Miquel Gimeno
Summary: Subtilisin Carlsberg, an alkaline protease from Bacillus licheniformis, can catalyze the synthesis of high molecular weight cationic alpha-poly-L-lysine and amphiphilic poly(alpha-L-lysine-co-L-phenylalanine) in organic solvent. The reaction is conducted in hydrophobic non-toxic 1,1,1,2-tetrafluoroethane solvent, and the low water environment limits hydrolysis of the peptide chains. The organic compressed fluid used in the reaction has low-pressure requirements and evaporates completely after the reaction, leaving no solvent residues. The resulting polypeptides show no cytotoxicity in human cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Article
Polymer Science
Manuel E. Martinez, Jose Rene Rangel-Mendez, Miquel Gimeno, Alberto Tecante, Gretchen T. Lapidus, Keiko Shirai
Summary: A chitosan reinforced with poly-epsilon-caprolactone thermoplastic composite was prepared and characterized in this study. The results showed that the material had high adsorption equilibrium capacities for Zn(II), Cu(II), Fe(II), and Al(III), and had minimal mass loss during the adsorption-desorption process.
Article
Food Science & Technology
Maria Dolores Diaz-Cervantes, Emma Gloria Ramos-Ramirez, Miquel Gimeno-Seco, Juan Alfredo Salazar-Montoya
Summary: The study aimed to optimize the extraction of chan seed oil using supercritical fluid and response surface methodology, and evaluate its physicochemical properties and quality. The optimal extraction conditions were determined to be 450 bar, 80 degrees C, and 193 min, resulting in a yield of 9.21% and recovery of 62.36%. The oil exhibited good stability and high content of omega 3, 6, and 9 fatty acids.
FOOD ANALYTICAL METHODS
(2023)
Article
Chemistry, Applied
A. Navarro, M. Gimeno, S. Alatorre-Santamaria, A. Navarro-Ocana
Summary: In this study, it was found that the enzymatic extract of Raphanus sativus var sativus exhibited peroxidase activity and catalyzed oxidative coupling of phenols, resulting in the formation of vanilloid and alkyl ester dimers.
REVISTA MEXICANA DE INGENIERIA QUIMICA
(2022)
Article
Materials Science, Biomaterials
Carmen G. Hernandez-Valencia, Miguel A. Hernandez-Valdepena, Alfredo Vazquez, Luis Cedeno-Caero, Jose Pedraza-Chaverri, Roberto Sanchez-Sanchez, Miquel Gimeno
Summary: The poly(gallic acid) grafted with -L-Lysine (LL) exhibits excellent antimicrobial activity against gram-positive Staphylococcus aureus and gram-negative Escherichia coli strains. The interaction between the positive charged polymer and the negatively charged bacterial membranes suggests a mechanism of pore-formation and translocation. Furthermore, the LL hybridized polymer shows antioxidant activity, high hemolysis concentration, and no cytotoxicity on human dermal fibroblasts, making it a promising antimicrobial material with potential cell preservation effects.
BIOMATERIALS ADVANCES
(2022)
Article
Cell Biology
Yessica Zamudio-Cuevas, Valentin Martinez-Lopez, Ivan Alejandro Lujan-Juarez, Nathalie Montano-Armendariz, Karina Martinez-Flores, Javier Fernandez-Torres, Miquel Gimeno, Roberto Sanchez-Sanchez
Summary: This study evaluated the effect of poly-gallic acid (PGAL) in an in vitro model of oxidation and synovial inflammation induced by monosodium urate (MSU) crystals. The results showed that PGAL reduced cell death and vesicle generation, decreased oxidative stress, and inhibited the inflammatory response. PGAL exhibited a cytoprotective effect against MSU-induced damage in synoviocytes.
Article
Food Science & Technology
Lazaro Gabriel Trujillo-Juarez, Oscar Hernandez-Melendez, Miguel Gimeno, Jesus Gracia-Fadrique, Eduardo Barzana
Summary: Citrus peels are a rich source of essential oils (EOs), which are widely used in the food, cosmetics, and pharmaceutical industries. This study proposed a method for extracting EOs from grapefruit peel using a pilot-scale twin-screw extruder, showing high productivity and energy efficiency. The produced EOs met international quality standards and can be used for commercial purposes.
ACS FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Polymer Science
Zhongming Yuan, Hiroko Yamamoto, Shinji Kanehashi, Takeshi Shimomura
Summary: This study reports the synthesis of a flexible light-emitting polymer film using a solution blending method. The selective positioning of MEH-PPV molecules in the PS phase of the film was confirmed. The film exhibited a nanometer-scale luminescent domain with low aggregation quenching and showed good elastomeric characteristics.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2024)
