Article
Biochemical Research Methods
Preeyanut Butmee, Gamolwan Tumcharern, Chomphunuch Songsiriritthigul, Marie Jose Durand, Gerald Thouand, Margaret Kerr, Kurt Kalcher, Anchalee Samphao
Summary: This study fabricated a novel enzymatic electrochemical biosensor for detecting glyphosate-based acid phosphatase inhibition. The biosensor functioned by measuring oxidation current through enzymatic reactions, and was successfully applied for selective analysis of glyphosate in actual samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jiaxi Gao, Xiaoyu Hua, Ruo Yuan, Qiong Li, Wenju Xu
Summary: An amplified and renewable electrochemical biosensor was developed through bienzymatic cascade catalysis using glucose oxidase and horseradish peroxidase, confined in a functional Y-shaped DNA nanostructure oriented by a dual-thiol-ended hairpin probe. The method showed high sensitivity in detecting sequence-specific biomarker DNA related to Alzheimer's disease.
Article
Chemistry, Analytical
Yuri D. Ivanov, Vadim Y. Tatur, Ivan D. Shumov, Andrey F. Kozlov, Anastasia A. Valueva, Irina A. Ivanova, Maria O. Ershova, Nina D. Ivanova, Igor N. Stepanov, Andrei A. Lukyanitsa, Vadim S. Ziborov
Summary: This study investigates the effects of an electromagnetic field induced by a rotating metallic cone on the properties of horseradish peroxidase (HRP) enzyme. Atomic force microscopy and spectrophotometry are used to analyze the enzyme at both molecular and macroscopic levels. The results show that the rotation of the cone causes the disaggregation and formation of higher-order aggregates of the enzyme, without affecting its enzymatic activity. These findings have implications for the development and interpretation of enzyme-based biosensors with rotating elements.
Article
Biochemistry & Molecular Biology
Yu Xiong, Chao Wang, YuanFei Wu, Chunhua Luo, Dongping Zhan, Shizhen Wang
Summary: Using two-dimensional metal-organic layers (MOLs) as electron transfer mediators helps to enhance the sensitivity, reproducibility, and stability of redox enzyme-based sensors.
Article
Electrochemistry
Lu Yang, Nan Wu, Rongrong Bai, Minjiao Chen, Weiliang Dong, Jie Zhou, Min Jiang
Summary: We report a facile strategy for the detection of pyruvate in fermentation progress based on a non-enzymatic decarboxylation reaction. The as-prepared biosensor demonstrated high electrocatalytic performance and was successfully used for the determination of pyruvate in real microbial fermentation processes. This work provides a new approach for the design and preparation of portable sensors for indirect pyruvate detection.
ELECTROCHIMICA ACTA
(2022)
Article
Engineering, Environmental
Jiacong Wu, Xinnan Ma, Chunmei Li, Xiangtong Zhou, Juan Han, Lei Wang, Hongjun Dong, Yun Wang
Summary: A novel photon-enzyme cascade catalysis system, composed of hybrid HRP-CN/Cu-3(PO4)(2) nanoflowers, was successfully constructed. This system exhibits excellent immobilized enzyme enzymatic properties and better tolerance to extreme conditions compared to free HRP. It shows outstanding degradation performance for bisphenol A (BPA) and can replace traditional systems for the harmless treatment of BPA in wastewater.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Luis Angel Zambrano-Intriago, Celia G. Amorim, Alberto N. Araujo, Dmitrij Gritsok, Joan Manuel Rodriguez-Diaz, Maria C. B. S. M. Montenegro
Summary: This work describes the development of an affordable, simple, and accurate electrochemical biosensor for the determination of glyphosate (GLY) in river and drinking water samples. The biosensor showed high reproducibility, excellent analytical performance, repeatability, and accuracy. It proves to be selective against other pesticides, organic acids, and inorganic salts.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Lucia M. E. Ibarra Bouzada, Silvia R. Hernandez, Silvina Kergaravat
Summary: Two environmentally friendly methods based on enzymatic inhibition were compared for rapid determination of glyphosate in commercial formulations, utilizing spectrophotometry (UV/Vis-SPD) and spectrofluorimetry (FLD). FLD showed greater sensitivity, simplicity, and speed compared to UV/Vis-SPD, with lower limits of detection of 4 μg/L. These methods can be applied to detect glyphosate in environmental samples from commercial formulations.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jinling Fu, Jie Wu, Rui Zhang, Qiang Wu, Huangxian Ju
Summary: A universal and highly sensitive electrochemical biosensing strategy utilizing a triplet nanostructure-mediated dendritic hybridization chain reaction (HCR) was developed for the detection of Dengue virus (DENV) nucleic acid, achieving ultrasensitive electrochemical detection with the ability to distinguish single-base mutation. The stable three-dimensional dendritic nanostructure with rigid double helix ensured the excellent performance of the designed electrochemical biosensing strategy. The proposed method showed good extendability for other nucleic acids, providing a promising candidate for nucleic acid detection in early clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Fengmao Li, Yufan Fu, Hao Yang, Yunming Tang
Summary: Luteolin effectively inhibits peroxidase activity in a noncompetitive manner, forming a static complex driven by hydrogen bond and hydrophobic interaction. Interaction between luteolin and peroxidase results in intrinsic fluorescence quenching, structural and conformational alterations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Yue He, Jing Zhou, Ruijie Fu, Yanlin Liu, Yiwen Wang, Haoran Liu, Jing Zhao, Yongliang Cui, Bining Jiao
Summary: An ultrasensitive colorimetric method for detecting genomic DNA of Alternaria from citrus fruit samples was described in this work, utilizing a system of five groups of reporter probes that form a DNA-heptamer sandwich structure on magnetic beads. The method has a low detection limit and is suitable for Alternaria genetic detection with its high sensitivity.
Article
Engineering, Chemical
Jiacong Wu, Xinnan Ma, Ting He, Juan Han, Yan Zhu, Chunmei Li, Yun Wang
Summary: The structural limitation of the photocatalyst itself hinders the increase in enzyme loading amount in the photo-enzyme coupling system. A g-C3N4-based photocatalyst ACN(45) with high enzyme loading amount was synthesized to address this issue. The channels in ACN(45) matched well with the size of the enzyme molecules, enabling a loading amount 6.3 times higher than pure g-C3N4. The ACN(45) channels also improved enzyme activity and stability, and reduced enzyme leaching. The high loading amount of the photo-enzyme coupling system enhanced the photocatalytic degradation of BPA, achieving a degradation rate 5.9 times higher than pure g-C3N4 within 1 hour. These findings provide a simple approach to design a photo-enzyme coupling system with high enzyme loading and stability.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Xiangyu Ma, Kun Qian, Onome Ejeromedoghene, Martha Kandawa-Schulz, Wei Song, Yihong Wang
Summary: A label-free electrochemical platform for the determination of microRNA-21 was developed using a thionine functionalized Fe-N-C nanocomposite. The high sensitivity and specificity of the biosensor were achieved through differential pulse voltammetry (DPV) testing of the strong electrochemical reduction signal of thionine. The sensitivity of the biosensor was further enhanced by utilizing a catalytic hairpin assembly (CHA) reaction.
Article
Biochemistry & Molecular Biology
Jakub Zdarta, Oliwia Degorska, Katarzyna Jankowska, Agnieszka Rybarczyk, Adam Piasecki, Filip Ciesielczyk, Teofil Jesionowski
Summary: Immobilization of horseradish peroxidase in sodium alginate capsules and further trapping in poly(vinyl chloride) electrospun fibers enhances enzyme stability, reusability, catalytic properties, and substrate affinity, making it a promising tool for enzymatic conversion of pharmaceutically active ingredients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Guangming Li, Hao Liu, Tianding Hu, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: This study reports a dimensionality engineering strategy to convert conventional 2D Fe-N-4 centers into 3D structures by integrating oxidized sulfur functionalities onto the carbon plane. The results suggest that oxidized sulfur functionalities can serve as binding sites for assisting substrate orientation and facilitating the desorption of H2O, resulting in an outstanding specific activity of up to 119.77 U mg(-1), which is 6.8 times higher than that of conventional FeN4C SAzymes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Michel Rocha Baqueta, Augusto Cesar Costa-Santos, Ana Paula Rebellato, Gisele Marcondes Luz, Juliana Azevedo Lima Pallone, Federico Marini, Alexsandro Lara Teixeira, Douglas N. Rutledge, Patricia Valderrama
Summary: This study reports the essential element composition of new Brazilian Canephora coffees and applies chemometric tools for analysis. The results demonstrate the suitability of this method for origin and species discrimination based on the inorganic fraction of coffee.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Mengjie Li, Jiapeng Wang, You Zhou, Yang Chen, Lingying Xia
Summary: A novel photoelectrochemical biosensor based on gold nanoparticles and specific structures was developed for highly sensitive detection of mercury ion. The multiple sandwich structures hindered electron supply and light harvesting, resulting in a weakened PEC signal, while the specific structures extinguished the PEC signal, enabling quantitative detection of Hg2+. The sensor exhibited high selectivity and stability, with potential applications in environmental detection, biological analysis, and medical research.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Mohamed A. Korany, Rasha M. Youssef, Marwa A. A. Ragab, Mostafa A. Afify
Summary: A stability-indicating fit-for-purpose method was developed for the determination of HC, TL, and PS in syrup dosage form. The method successfully separated CL from THY and quantified the analytes using HPLC-DAD. The robustness of the method was assessed through statistical analysis and self-validation.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Lingling Lin, Minyu Li, Ping Li, Chenqing Ye, Huanglong Zhuang, Shaohuang Weng, Feng Chen
Summary: This study developed a highly sensitive and selective electrochemical sensor for simultaneous detection of dopamine and uric acid. The sensor exhibited excellent anti-interference and stability, and could accurately measure dopamine and uric acid in human urine samples.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Peifang Chen, Caiyun Jiang, Zhouping Wang, Hong-zhen Lian, Xiaoyuan Ma
Summary: In this study, a high-performance SERS-active aptasensor was successfully developed for the quantitative analysis of AFB1 in cereal grains, which is of great significance in the field of food safety.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
E. Abas, C. Marina-Montes, C. Perez-Marin, J. Puimedon, J. Anzano
Summary: This study conducted a comprehensive measurement of natural and anthropogenic radionuclides in soil, water, and air in different locations around Deception Island in Antarctica. The results revealed significant levels of 210Pb and anthropogenic 137Cs in soil samples near old human settlements and facilities, as well as detectable levels of 137Cs and 60Co in water samples from old hunting areas. The findings also suggested potential resuspension processes based on higher-than-expected air quality results.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Joao Franciso Allochio, Nayara A. dos Santos, Nathalia dos S. Conceicao, Clara S. D. Baptista, Keyller B. Borges, Valdemar Lacerda Jr, Wanderson Roma
Summary: This study developed a fiber spray ionization mass spectrometry (FSI-MS) method, which shows great potential in detecting illicit drugs. Compared with paper spray ionization mass spectrometry (PSI-MS), FSI-MS demonstrates higher sensitivity in COC detection.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Dina A. Ahmed, Ola G. Hussein, Mamdouh R. Rezk, Mohamed Abdelkawy, Yasmin Rostom
Summary: The main goal of this study was to develop two green chromatographic techniques, HPLC and TLC, for the determination of alcaftadine (ALF) in pharmaceutical formulation and biological samples. The developed HPLC technique showed high efficiency and minimal environmental impact, successfully identifying ALF in the presence of its degradate. The TLC method provided a stability indicating study for ALF and was determined to be safe, green, and eco-friendly.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Abdullah Taner Bisgin
Summary: A green, speedy, and handy method for separation and determination of four commonly used food colorants was established using vortex assisted sequential-simultaneous liquid phase micro-extraction (VA-SS-LPME). The method does not require any instrumental chromatographic techniques. The procedure includes sequential separation steps using different extractants and simultaneous UV-vis spectrophotometric determination steps. The method is environmentally friendly and applicable for determination of colorants in food and pharmaceuticals.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Yifan Zhang, Tong Zhang, Wenjing Ba, Li Liu, Yuan Rao, Xiaodan Zhang, Hanhan Zhang, Xiu Jin
Summary: This paper proposes a novel spectral transfer modelling method based on neural network architecture searching for the asymptomatic prediction of Akizuki pear cork spot disorder. The experiments show that the model by this method is more effective, achieving an accuracy of 82.61%.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Krzysztof Greda, Maja Welna, Anna Szymczycha-Madeja, Pawel Pohl
Summary: For the first time, a dispersive micro-solid phase extraction (D-mu SPE) based on graphene oxide was used for the ultrasensitive determination of Cd. With this method, Cd can be efficiently extracted from a mixture of alkali and alkaline earth metals without the need for an elution step. The combination of D-mu SPE and SAGD OES significantly reduces matrix effects and improves the tolerance of Cd to alkali metals. The method shows simplicity in the measurement system and the commercially available nanosorbent used.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
P. K. Fathima Anjila, G. R. Tharani, Anand Sundaramoorthy, Venkat Kumar Shanmugam, Karthikeyan Subramani, Shanmugavel Chinnathambi, Ganesh N. Pandian, Vimala Raghavan, Andrews Nirmala Grace, Singaravelu Ganesan, Mangaiyarkarasi Rajendiran
Summary: This work proposes a rapid and sensitive spectroscopic method for detecting melamine in food products using rare-earth Terbium-doped Graphene Quantum Dots (Tb-GQDs) synthesized by microwave and simple chemical conjugation methods. The synthesized Tb-GQDs show a spherical shape with a size of approximately 6 nm and a quantum yield of 52%. The maximum detection limit for melamine concentration in milk and milk products is found to be 0.31 μM. The dual emitting Tb-GQDs provide a sensitive and effective platform for real-time detection of melamine in food samples, particularly in milk, and show promising potential in the food industry.
MICROCHEMICAL JOURNAL
(2024)
Review
Chemistry, Analytical
Piyush Dey, Mandeep Kaur, Akhil Khajuria, Dilmeet Kaur, Manpreet Singh, Hema Kumari Alajangi, Neha Singla, Gurpal Singh, Ravi Pratap Barnwal
Summary: Environmental issues, such as the uncontrolled release of dangerous pollutants, have significant impacts on human health. Traditional methods for detecting pollutants require specialized personnel and equipment, but nanotechnology has improved the performance of biosensors. This article discusses the use of nanotechnology in pollutant detection and the associated techniques.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Sa Dong, Qiuyun Shi, Lingjun Guan, Yulong Wang, Pengyan Liu, Cunzheng Zhang, Jianguo Feng
Summary: In this study, a highly sensitive indirect competitive chemiluminescence enzyme immunoassay (icCLEIA) method was developed for the determination of sodium pentachlorophenolate (PCP-Na) and its metabolite pentachlorophenol (PCP). The method showed high specificity and affinity with a detection limit of 0.0032 ng/mL for PCP-Na and 0.0192 ng/mL for PCP. The method had no cross reaction with other chlorophenols compounds except for PCP. The average recoveries in chicken, pork and fish samples were 81.3-105.8% with low coefficient of variation (CV).
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Analytical
Palaniappan Ilayaraja, Murugan Manivannan, Paramasivam Parthiban
Summary: Nirmatrelvir, an antiviral drug used for the treatment of COVID-19, has been recommended by the World Health Organization. We developed an optimized analytical method for the quantification of Nirmatrelvir and its products, with high sensitivity and a wide linear range. The method also meets the requirements for greenness evaluation.
MICROCHEMICAL JOURNAL
(2024)