Article
Chemistry, Analytical
Shuaibing Yu, Jian Zhang, Yaodong Hu, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: The development of a convenient and efficient assay for miRNA-21 as a lung cancer marker is crucial for early cancer prevention. In this study, an electrochemical biosensor using click chemistry and photocatalytic atom transfer radical polymerization (photo-ATRP) was successfully fabricated for miRNA-21 detection under blue light excitation. The biosensor, based on hairpin DNA recognition probe, achieved signal amplification by depositing electroactive monomers on the electrode surface, exhibiting sensitivity, precision, and selectivity for miRNA-21 with different base mismatches. It showed a linear relationship in the range of 10 fM to 1 nM (R-2 = 0.995) and a detection limit of 1.35 fM. The biosensor also demonstrated anti-interference performance when analyzing RNAs in serum samples. It is a promising tool for the analysis of complex samples, offering the advantages of low cost, specificity, and anti-interference ability.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Qianqian Qi, Yutong Zhang, Wei Xiong, Xingyu Liu, Shuangyu Cui, Xiaofang Ye, Kaisong Zhang, Tian Tian, Xiang Zhou
Summary: In this study, the norbornene-tetrazine ligation chemistry is used to develop clickable RNA switches in biological contexts, and its potential applications are explored. The application of RNA-based norbornene-tetrazine ligation chemistry in controlling CRISPR systems and manipulating gene editing in human cells is successfully demonstrated.
Article
Chemistry, Multidisciplinary
Alex Gibney, Raphael E. F. de Paiva, Vandana Singh, Robert Fox, Damien Thompson, Joseph Hennessy, Creina Slator, Christine J. McKenzie, Pegah Johansson, Vickie McKee, Fredrik Westerlund, Andrew Kellett
Summary: In this study, a Cu-catalysed azide-alkyne cycloaddition reaction produced a 1,2,3-triazole linker that was used to create Cu-binding artificial metallo-nucleases (AMNs). A bioactive C-3-symmetric ligand called TC-Thio was developed using biologically inert reaction partners. The ligand formed Cu-II and Cu-I complexes through Cu coordination, and showed potent DNA binding and cleaving abilities. Mechanistic studies revealed that DNA recognition occurred at the minor groove, leading to oxidative damage through a pathway dependent on superoxide and peroxide. Single molecule imaging demonstrated that the complex caused DNA damage recognized by base excision repair enzymes, comparable to the clinical drug temozolomide.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemical Research Methods
Xiaoyu Guo, Luyao Chen, Peipei Li, Xiaofei Li, Jianfeng Wang, Liang Guo, Huaixia Yang
Summary: The study constructed an ECL biosensor for sensitively detecting TMV RNA by combining click chemistry with ARGET-ATRP for the first time. The sensor showed a good linear relationship between the ECL signal and the logarithm of tRNA concentration in the range of 0.1 pM-10 nM, with a detection limit of 2.61 fM. Additionally, the strategy can identify mismatched bases and perform well in recovery assays in real samples.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Joseph Hennessy, Brionna McGorman, Zara Molphy, Nicholas P. Farrell, Daniel Singleton, Tom Brown, Andrew Kellett
Summary: A click chemistry approach was used to generate a library of Pt-II-TFO hybrids, which were able to enhance target binding and discrimination between target and off-target sequences by incorporating modifications of thiazole orange. This method offers a potential solution to the limitations of clinical platinum(II) therapeutics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemical Research Methods
Alexandra Bristiel, Manon Cadinot, Mathieu Pizzonero, Frederic Taran, Dominique Urban, Raphael Guignard, Dominique Guianvarc'h
Summary: The researchers developed a facile method for conjugating relevant probes, ligands, or delivery agents onto oligonucleotides (ONs), which is important for both fundamental studies in chemical biology and improving the pharmacology of ONs in medicinal chemistry. They introduced novel 2'-functionalized nucleoside phosphoramidite monomers that contain bioorthogonal methylcyclopropene or sydnone moieties for the first time to ON solid-phase synthesis, allowing traceless ON postsynthetic modifications with reactive complementary probes through inverse electron-demand Diels-Alder reactions or strain-promoted sydnone-alkyne cycloaddition. These findings expand the bioorthogonal phosphoramidite building blocks and provide a new method for generating labeled ONs.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Polymer Science
Steffen A. A. Busche, Stefan Peplau, Luc Zuhse, Desiree Steimer, Dennis D. D. August, Hans G. G. Boerner
Summary: The selective ligation of stable norbornene (Nb) with an asymmetric tetrazine (Tz) is used to connect polymer segments and produce block copolymers. The Tz/Nb inverse electron-demand Diels-Alder reaction (IEDDA) is compared with the azide (Az)-norbornene Huisgen 1,3-cycloaddition (CA) to ligate end-functionalized polymers. The Tz/Nb IEDDA shows better conversion and cleaner reactions compared to the Az/Nb CA, making it a robust and potentially scalable method for ligation.
Article
Polymer Science
Alexis Theodorou, Petros Mandriotis, Athina Anastasaki, Kelly Velonia
Summary: The scope of amphiphilic protein-polymer bioconjugates can be expanded through an oxygen tolerant, photoinduced RDRP method, allowing the preparation of protein-polymer conjugates with different polymer moieties. By optimizing reaction conditions, well-defined protein-polymer conjugates with specific structures and functions can be obtained.
Article
Chemistry, Analytical
Yan Qi, Yuqing Zhai, Wenjiao Fan, Wei Ren, Zhengping Li, Chenghui Liu
Summary: The versatile single magnetic nanoparticle-confined, click chemistry-actuated digital DNA walker machine enables absolute quantification of microRNA, reflecting the initial miRNA input digitally and faithfully by counting fluorescent-positive MNPs without external calibration. This design expands the toolbox for digital biosensing with a new digital signaling concept.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Leona L. Rodrigues, Aaron S. Micallef, Michael C. Pfrunder, Vinh X. Truong, John C. McMurtrie, Tim R. Dargaville, Anja S. Goldmann, Florian Feist, Christopher Barner-Kowollik
Summary: A highly efficient ligation system based on visible light-induced rearrangement was introduced, generating a thiophenol for rapid thiol-Michael additions without the need for additives. The self-catalyzed ligation through pyridine mediated deprotonation of the photochemically generated thiophenol was described, leading to additive-free thiol-Michael reactions. The study explored various reaction conditions, solvents, and substrates, showcasing excellent end group fidelity in polymer modification.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Grant A. Knappe, Eike-Christian Wamhoff, Benjamin J. Read, Darrell J. Irvine, Mark Bathe
Summary: Researchers have developed a versatile DNA origami functionalization and characterization platform for biomedical applications. By utilizing click chemistry, they achieved quantitative in situ conversion of various moieties. This platform allows for broader access to covalently functionalized DNA origami.
Article
Biochemistry & Molecular Biology
Vladimir Burilov, Egor Makarov, Diana Mironova, Elza Sultanova, Islamiya Bilyukova, Kevser Akyol, Vladimir Evtugyn, Daut Islamov, Konstantin Usachev, Timur Mukhametzyanov, Svetlana Solovieva, Igor Antipin
Summary: This paper presents the synthesis of new water-soluble calixarene triazoles and investigates their aggregation behavior, as well as their binding and compaction abilities towards DNA. The results suggest that the tetradecyl-substituted calix[4]arene exhibits the best performance in terms of DNA binding and compaction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Mohammad Javad Taghizadeh, Ali Saadatinia
Summary: Well-defined star, di-block, and tri-block copolymers were synthesized using ATRP and click reaction. The addition of these copolymers to epoxy resin resulted in smaller nanostructures and improved thermomechanical properties. The star copolymer/epoxy resin formulation exhibited the highest tensile strength, elongation at break, and thermal properties compared to the di-block and tri-block copolymer/epoxy resin formulations.
JOURNAL OF POLYMER RESEARCH
(2022)
Article
Polymer Science
Sumit Dadhwal, Arnold Lee, Shailesh K. Goswami, Sarah Hook, Allan B. Gamble
Summary: In this study, a self-immolative PEG-aryl azide block copolymer and a non-responsive 4-fluoroaryl block copolymer were successfully synthesized through systematic investigation of the RAFT and ATRP methods. The ATRP method provided the desired polymers in a highly controlled manner, while the RAFT conditions led to higher levels of aryl azide polymer degradation. Stimuli-responsive aryl azide polymers/nanoparticles demonstrated non-cytotoxicity up to 200 μg/ml concentrations in preliminary in vitro cell viability studies.
JOURNAL OF POLYMER SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Mathias B. Danielsen, Niels Johan Christensen, Per T. Jorgensen, Knud J. Jensen, Jesper Wengel, Chenguang Lou
Summary: Cationic moieties attached to oligonucleotides increased binding affinity and stability, with the 2'-amino-LNA analogue carrying a triaminated linker showing the most pronounced effect. Molecular modeling revealed salt-bridge formation between positively charged polyamine moieties and the dsDNA targets, resulting in the observed triplex stabilization. All investigated monomers showed increased resistance against 3'-nucleolytic digestion.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Polymer Science
Wang-Kai Xiang, Qing Xie, Shan-Shan Xu, Chen-Xuan Sun, Cheng-Tao Yu, Ying Zheng, Peng-Ju Pan
Summary: Stereocomplex crystallization is an effective method to improve the physical properties of stereoregular polymers. This study investigates the effects of PDLA fraction and crystallization temperature on the crystallization kinetics and polymorphic crystalline structure using asymmetric blends of PLLA and PDLA. The results show that a high PDLA fraction and low temperature favor the crystallization of beta-form homocrystals.
CHINESE JOURNAL OF POLYMER SCIENCE
(2022)
Review
Polymer Science
Shanshan Xu, Jian Zhou, Pengju Pan
Summary: This work summarizes important advances in research on both non-crystallizable and crystallizable polymers regarding their stretching behavior near T-g. Through case studies on typical examples, the review systematically presents structural changes, including chain orientation/relaxation kinetics, dynamic network evolution, and stretch-induced phase transition. A discussion on the influence of these structural evolutions on essential end-use properties of materials is also included, aiming to provide guidance for future stretching-related studies.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2022)
Review
Chemistry, Multidisciplinary
Thiruchelvi Pulingam, Parisa Foroozandeh, Jo-Ann Chuah, Kumar Sudesh
Summary: This review article discusses the different methods employed for the production of polymeric nanoparticles (PNPs) and their physicochemical properties influenced by experimental parameters. The tailored characteristics of PNPs can be utilized as drug carriers for targeted therapies in hospitals for point-of-care diagnostics.
Article
Chemistry, Multidisciplinary
Surachada Chuaychob, Masahiro Fujita, Mizuo Maeda
Summary: In this study, a new sensing platform for the biomolecule thrombin was developed using G-quadruplex (G4) DNA-functionalized gold nanoparticles (AuNPs). Thrombin was detected by the aggregation-induced color change of the colloidal system, which was attributed to the reduction in steric repulsion caused by the mobility and flexibility of G4 DNA. This sensor showed high specificity to thrombin and exhibited a tunable detection sensitivity and analytical performance.
Article
Polymer Science
Yucheng He, Dan Liu, Kefeng Xie, Wenqing Xu, Pengju Pan, Wenbing Hu
Summary: The relaxation of structure in poly(lactic acid) blends promotes crystal nucleation, and the annealing at different temperatures has different effects on the nucleation process.
Article
Polymer Science
Jianfei Xia, Shanshan Xu, Ying Zheng, Jian Zhou, Chengtao Yu, Guorong Shan, Yongzhong Bao, Pengju Pan
Summary: This study systematically investigates the effect of comonomer defects on the crystalline structure and phase transition behavior of copolyamides. The results show that the copolyamides exhibit composition-dependent Brill transition temperatures, with a pseudoeutectic characteristic in the intermediate composition range. The copolyamides with pseudoeutectic compositions undergo an irreversible Brill transition, with the γ phase remaining unchanged upon cooling from melt. The γ-alpha transition of pseudoeutectic copolyamides is found to be temperature-dependent.
Article
Nanoscience & Nanotechnology
Li Kong, Luyang Wang, Yali Shi, Lin Peng, Xingguo Liang, Guoqing Wang, Hideyuki Mitomo, Tohru Takarada, Kuniharu Ijiro, Mizuo Maeda
Summary: This study demonstrates the development of adaptable plasmonic nanoactuators that can be precisely controlled in uncontrolled environments. By manipulating temperature, light, or pH, these nanoactuators can release stored elastic energy and perform controllable movements.
ACS APPLIED NANO MATERIALS
(2022)
Article
Microbiology
Jia Hui Wan, Lee-Mei Ng, Soon Zher Neoh, Rei Kajitani, Takehiko Itoh, Susumu Kajiwara, Kumar Sudesh
Summary: Aquitalea sp. USM4, isolated from Malaysia, is a bacteria that produces polyhydroxyalkanoate (PHA) with biodegradability and biocompatibility features. The genome analysis revealed important genes involved in PHA production, and the heterologous expression of phaC1 from Aquitalea sp. USM4 in Cupriavidus necator PHB(-)4 resulted in the incorporation of six different types of PHA monomers. This study provides valuable genomic information for understanding PHA biosynthesis.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Luyang Wang, Zhiyu He, Qianyuan Chen, Guoqing Wang, Xingguo Liang, Tohru Takarada, Mizuo Maeda
Summary: The increasing use of disposable nanomaterials and the resulting nanowastes with potential environmental risks have become a global public concern. However, the recovery of useful materials from nanowastes is currently limited due to their tiny size, high physicochemical activity, and unpredictable aggregation. A new recycling strategy for nanoprobes using alcoholic solvent refreshing has been developed, which allows for the restoration of individual nanoprobes by denaturalizing DNA to release heavy metal ions. This method not only enables sensitive detection but also achieves high recycling efficiency (>80%) while preserving the detection performance of the nanoprobes.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Chanaporn Trakunjae, Antika Boondaeng, Waraporn Apiwatanapiwat, Phornphimon Janchai, Soon Zher Neoh, Kumar Sudesh, Pilanee Vaithanomsat
Summary: In this study, the production of P(3HB-co-3HHx) copolymers with higher 3HHx monomer compositions was enhanced using response surface methodology (RSM). A maximum of 3.6 +/- 0.4 g/L of P(3HB-co-3HHx) with 4 mol% 3HHx compositions was obtained in flask scale experiments under the optimized condition. Scaling up the fermentation in a 10L-stirrer bioreactor yielded a higher 3HHx monomer composition (5 mol%). The properties of the produced polymer were similar to marketable P(3HB-co-3HHx), making it suitable for a wide range of applications.
SCIENTIFIC REPORTS
(2023)
Article
Polymer Science
Iffa Farahin Jeepery, Tatsuya Goto, Kumar Sudesh, Hideki Abe
Summary: The selected biodegradable block copolymer, atactic poly(3-hydroxybutyrate) (PHB)-b-poly(& epsilon;-caprolactone) (PCL) diblock copolymer, acted as a compatibilizer for the immiscible blends of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH)/PCL. The impact of the copolymer on the blend morphologies and mechanical properties depended on the blend composition. The mechanical properties of the PHBH/BCP blends were significantly improved when the copolymer was used as one of the blend components. The presence of the copolymer in the blend systems reduced the enzymatic degradation rate by PHB depolymerase.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Polymer Science
Chenxuan Sun, Xuekuan Ma, Lingling Ni, Mengru Ding, Jianfei Xia, Ying Zheng, Chengtao Yu, Bao Wang, Pengju Pan
Summary: Side substitution is an effective method for the chemical modification of linear polyesters, and the presence of side groups has a profound effect on the crystalline structure and phase transition of semicrystalline polyesters. This study synthesized polyesters with different side groups and investigated the effects of these groups on the crystal polymorph and phase transition of the substituted polyesters.
Article
Chemistry, Analytical
Zhiyu He, Qianyuan Chen, Shansen Ding, Guoqing Wang, Tohru Takarada, Mizuo Maeda
Summary: Researchers have developed a rapid colorimetric approach for detecting ochratoxin A (OTA) in Baijiu using DNA-functionalized gold nanoparticles (DNA-AuNPs). The method relies on the competition between OTA and DNA on the AuNP surface, resulting in a turn-on color when OTA is present. By suppressing DNA hybridization and using an alcohol solution, the DNA-AuNPs show improved reproducibility and high sensitivity to OTA. The method has a detection limit of 88 nM and can be completed in less than 17 minutes without sample pretreatment.
Article
Agricultural Engineering
Jia Yun Chang, Taufiq Ahmad Syauqi, Kumar Sudesh, Si Ling Ng
Summary: Polyhydroxyalkanoates (PHAs) serve as effective biocarriers for biofilm development, showing excellent attachment performance and stable biofilm structure in acid orange 7 treatment.
BIORESOURCE TECHNOLOGY
(2024)
Article
Chemistry, Multidisciplinary
Ying Zheng, Shanshan Xu, Chengtao Yu, Pengju Pan
Summary: This article summarizes recent advances in SC crystallization and stereocomplexed materials of polylactic acid (PLA), including various approaches for controlling the polymorphic crystallization structure and the preparation of different types of stereocomplexed materials. These stereocomplexed materials exhibit excellent physical properties and can be further functionalized. Through this research, a practical platform has been established to uncover the relationships between crystalline structure, physical properties, and functions of stereocomplexed materials.
ACCOUNTS OF MATERIALS RESEARCH
(2022)
Article
Polymer Science
Yuanzhang Jiang, Xuyi Wang, Yanting Han, Dakai Gong, Yingchun Gu, Lin Tan
Summary: In this study, FeCl3 and poly(acrylic acid) (PAA) were doped into waterborne polyurethanes (WPUs) to create multifunctional materials with self-healing and antimicrobial properties. WPU/Fe/PAA-5.5, containing 5.5 wt % PAA, exhibited excellent mechanical properties, strong self-healing capabilities, and an impressive antimicrobial rate of over 90%. These supramolecular films were also highly recyclable through hot-pressing.
Article
Polymer Science
Longyu Tian, Min Wang, Guangming Liao, Baoliang Liu, Sujuan Zhang, Yucheng Sun, Zhen Meng, Jintao Zhang, Zaijun Lu
Summary: Two kinds of benzoxazine monomers were synthesized and used to prepare polybenzoxazine anion exchange membranes (AEMs) with quaternary ammonium functionalization. The LQPBZ AEM exhibited better ion conductivity and alkaline stability.
Article
Polymer Science
Claire A. Lemarchand
Summary: This study investigates the shock behavior of three different polymers through molecular dynamics simulations. The results reveal specificities in the shock behavior of polymers, including deviations from the linear relation between shock velocity and particle velocity, as well as differences in shear stress relaxation behind the shock front. It is found that the deviation of the Hugoniot locus is related to the change in the relative contribution of bonding and non-bonding potential energies, while polymers with higher glass transition temperatures exhibit slower shear stress relaxation.
Article
Polymer Science
Zengquan Liu, Xiaochun Yin, He Zhang, Shuo Gao, Qinglin Kuang, Yanhong Feng
Summary: A powder solid-state extension (PSSE) technology was proposed to address the high melt viscosity issue of UHMWPE. By uniaxial extension and sintering in the solid state, a highly self-reinforced material was prepared. X-ray diffraction and scattering techniques were used to study the structural evolution during the PSSE process, and a solid-phase induced transformation model was established.
Article
Polymer Science
Zhike Li, Anyu Luo, Rui Zhou, Xin Li, Haiyan Li
Summary: A novel high temperature resistance IL@SiO2 nanocapsules were successfully prepared and their friction and wear properties in PA6 composites were studied. The addition of IL@SiO2 nanocapsules reduced the friction coefficient and wear rate without affecting the mechanical properties of PA6. The synergy between the IL core and SiO2 wall in the nanocapsules improved the self-lubricating performance of the PA6 composites.
Article
Polymer Science
Xin Liu, Xuhong Guo, Qi Liao
Summary: In this study, an algorithm for accurately estimating viscosity is developed using molecular dynamics simulations and the Green-Kubo formula. This algorithm can be applied to complex systems with long correlations, such as macromolecular and biological simulation systems.
Article
Polymer Science
Hao Pu, Yun-Lei Hou, Jing -Zhou Chen, Dong -Lin Zhao
Summary: The use of modified graphene improves the interfacial adherence of carbon fibers to epoxy resin, resulting in enhanced interfacial and bending properties of CF and matrix in CF/EP composites, as well as increased interlaminar shear strength and flexural strength.
Article
Polymer Science
Wei-Chung Ke, Jin-Wei Lin, Manohar Reddy Busireddy, Yueh-Hsing Lee, Jiun-Tai Chen, Chain-Shu Hsu
Summary: This study introduces a crosslinkable monomer, TAIC, to synthesize three crosslinked polyimide films, which exhibit improved thermal, mechanical, and dielectric properties. The introduction of TAIC enhances the tensile strength, reduces the dielectric constant and dielectric loss, and decreases the coefficient of thermal expansion of the polyimide films.
Article
Polymer Science
Alex Kwasi Kumi, Ruiling Fan, Ye Chen, Yumei Zhang
Summary: The difference in leaching of amylopectin from cellulose/amylopectin/1-butyl-3-methylimidazolium chloride ([Bmim][Cl]) blends during regeneration in water and aqueous ethanol has been studied. Molecular dynamics simulations showed that the dissolution and regeneration mechanisms of amylopectin in [Bmim][Cl] are similar to cellulose in ionic liquids. Water regeneration leads to weak electrostatic interactions, resulting in high leaching of amylopectin. In contrast, ethanol-water interactions enhance electrostatic interactions among amylopectin chains, limiting amylopectin leaching in aqueous ethanol.
Article
Polymer Science
Xi Zeng, Junwei Zhou, Junbiao Peng, Chunsheng Zhang, Danling Wang, Yihu Song, Qiang Zheng
Summary: This study utilizes an oligomer deep eutectic solvent (DES) based on polyethylene glycol to regulate the vulcanization kinetics of rubber. The results show that the DES can accelerate vulcanization at low temperatures without affecting the crosslinking density and Mullins effect.
Article
Polymer Science
Qianqian Yue, Yongfei Peng, Xingjian Liu, Aihua He, Huarong Nie
Summary: The addition of metal deactivators can effectively suppress the aging of TBIR, prolong its lifespan, and have no negative effects on its physical and processing properties. These research findings are of great importance for improving the stability and aging resistance of polymers.
Article
Polymer Science
J. Gomez-Caturla, J. Ivorra-Martinez, R. Tejada-Oliveros, V Moreno, D. Garcia-Garcia, R. Balart
Summary: This work focuses on the development of environmentally friendly PLA formulations by using different esters derived from geraniol as plasticizers. The results show that these esters have good compatibility with PLA, and they can effectively enhance the elongation and plasticity of PLA, reduce its glass transition temperature, and slightly improve its water absorption capabilities.
Article
Polymer Science
Dandan Li, Zhaohui Lu, Zhao Ke, Ke Xu, Fengna Dai, Youhai Yu, Guangtao Qian, Chunhai Chen
Summary: In this study, cross-linked PI aerogel membranes with low dielectric constant and high moisture resistance were prepared by co-polymerization and scraping coating technology. The incorporation of fluorinated blocks and benzimidazole ring structures resulted in novel PI aerogel membranes with fascinating dielectric properties and outstanding moisture resistance.
Article
Polymer Science
Zhaoyang Chu, Qing Zhang, Haihua Luo, Han Zhou, Fapei Zhang, Wei Chen, Wenhua Zhang
Summary: Multi-physical fields solution processing strategy is a universal and facile method for preparing various conjugated polymer films and high-performance devices. In this study, we employed a combined microfluidic flow and ultrasonication strategy (FU) for processing CP solutions, and found a pronounced synergetic effect in promoting the pre-ordering of chains in solution. The conformation order and anisotropy of the solution were revealed through various characterizations. A non-classical nucleation model for polymer crystallization in non-equilibrium solution processing was confirmed. The roles of microfluidic flow and ultrasonication in chain aggregation and crystallization were addressed through multi-physical simulations. Compared to pristine solutions, the FU strategy showed improved solution anisotropy and crystallization kinetics, resulting in higher crystallinity in films and increased mobilities in OFET devices. The FU processing strategy provides a universal approach for regulating chain conformation and aggregation in conjugated polymer solutions.
