Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes

Duplication-Insertion Recombineering: a fast and scar-free method for efficient transfer of multiple mutations in bacteria

(2016) Joakim Näsvall et al.   NUCLEIC ACIDS RESEARCH

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

(2016) Ákos Nyerges et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli

(2015) Chris R. Reisch et al.   Scientific Reports

Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites

(2015) Selwyn Quan et al.   G3-Genes Genomes Genetics

Excision of Unstable Artificial Gene-Specific Inverted Repeats Mediates Scar-Free Gene Deletions in Escherichia coli

(2014) Crystal Jing Ying Tear et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

(2013) Xin-tian Li et al.   NUCLEIC ACIDS RESEARCH

Improved seamless mutagenesis by recombineering using ccdB for counterselection

(2013) Hailong Wang et al.   NUCLEIC ACIDS RESEARCH

Real-Time Evolution of New Genes by Innovation, Amplification, and Divergence

(2012) J. Nasvall et al.   SCIENCE

Activation of cryptic aminoglycoside resistance in Salmonella enterica

(2011) Sanna Koskiniemi et al.   MOLECULAR MICROBIOLOGY

Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome

(2011) Kathrin Blank et al.   PLoS One

Single-stranded heteroduplex intermediates in λ Red homologous recombination

(2010) Marcello Maresca et al.   BMC MOLECULAR BIOLOGY

Lambda Red Recombineering in Escherichia coli Occurs Through a Fully Single-Stranded Intermediate

(2010) J. A. Mosberg et al.   GENETICS

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Mutagenic inverted repeat assisted genome engineering (MIRAGE)

(2008) Nikhil U. Nair et al.   NUCLEIC ACIDS RESEARCH