Article
Engineering, Biomedical
Jeanette M. Metzger, Yuyuan Wang, Samuel S. Neuman, Kathy J. Snow, Stephen A. Murray, Cathleen M. Lutz, Viktoriya Bondarenko, Jesi Felton, Kirstan Gimse, Ruosen Xie, Dongdong Li, Yi Zhao, Matthew T. Flowers, Heather A. Simmons, Subhojit Roy, Krishanu Saha, Jon E. Levine, Marina E. Emborg, Shaoqin Gong
Summary: Genome editing of somatic cells using CRISPR offers hope for treating genetic disorders, including neurological diseases. However, the complex brain parenchyma and post-mitotic state of neurons pose challenges for efficient editing. Our study developed non-viral and biodegradable PEGylated nanocapsules that successfully delivered Cas9-sgRNA ribonucleoproteins to neurons in the mouse brain, resulting in safe and efficient genome editing.
Article
Biochemistry & Molecular Biology
Beatrix Paton, Elisabet Foguet-Romero, Manuel Suarez, Jordi Mayneris-Perxachs, Noemi Boque, Antoni Caimari, Nuria Canela, Pol Herrero
Summary: The consumption of diets rich in saturated fats is associated with higher mortality. Adopting healthy habits, such as adhering to a Mediterranean diet, has been shown to have a preventive effect on cardiovascular diseases and dyslipidemia. However, the effects of an suboptimal diet on brain function and structure are still poorly understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Sruti Rayaprolu, Sara Bitarafan, Juliet Santiago, Ranjita Betarbet, Sydney Sunna, Lihong Cheng, Hailian Xiao, Ruth S. Nelson, Prateek Kumar, Pritha Bagchi, Duc M. Duong, Annie M. Goettemoeller, Viktor Janos Olah, Matt Rowan, Allan Levey, Levi B. Wood, Nicholas T. Seyfried, Srikant Rangaraju
Summary: By using cell type-specific protein labeling, we can better study the proteomic differences between neurons and astrocytes, and identify potential factors related to neurological diseases.
NATURE COMMUNICATIONS
(2022)
Article
Psychiatry
Darren J. Fernandes, Shoshana Spring, Anna R. Roy, Lily R. Qiu, Yohan Yee, Brian J. Nieman, Jason P. Lerch, Mark R. Palmert
Summary: Studies have shown that exposure to a maternal high-fat diet in early-life can lead to structural changes in the brain that persist into adulthood, even after dietary modifications. These changes mainly occur in the extended amygdalar system, which may be related to reward-seeking and feeding behaviors.
TRANSLATIONAL PSYCHIATRY
(2021)
Article
Multidisciplinary Sciences
Changming Chen, Meiling Wen, Caixia Wang, Zhongwen Yuan, Ya Jin
Summary: In this study, a proteomic analysis of brain proteins in hyperlipidemic mice was conducted. The results showed significant changes in the expression of proteins related to lipid metabolism, neurological disorders, synaptic events, and nervous system development. Additionally, different expression patterns were observed in the two hemispheres of the brain, suggesting an asymmetric effect of hyperlipidemia on brain proteins.
Article
Biochemistry & Molecular Biology
Kate J. Claycombe-Larson, Amy N. Bundy, Terry Kuntz, Junguk Hur, Kathleen M. Yeater, Shanon Casperson, Dale C. Brunelle, James N. Roemmich
Summary: Maternal dietary fat and vegetable substitution can have significant effects on placental and fetal growth as well as fetal brain gene expression. The findings suggest that vegetable supplementation may protect against the negative impacts of a high-fat diet on fetal development.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yuanrui Hua, Wenshu Meng, Jing Wei, Yongtao Liu, Youhe Gao
Summary: Cardiovascular disease, particularly atherosclerosis, is the leading cause of death globally. Early diagnosis of atherosclerosis is crucial for effective management of cardiovascular disease. This study demonstrates that urinary proteomics can sensitively monitor changes in the body and has the potential to identify early biomarkers of atherosclerosis.
Article
Medicine, Research & Experimental
Stanley Ibeh, Nour-Mounira Z. Bakkar, Fatima Ahmad, Judith Nwaiwu, Chloe Barsa, Sarine Mekhjian, Mohammad Amine Reslan, Ali H. Eid, Hayat Harati, Sanaa Nabha, Yehia Mechref, Ahmed F. El-Yazbi, Firas Kobeissy
Summary: This study suggests that chronic high-fat diet worsens the outcomes of traumatic brain injury, leading to neurodegeneration and impaired brain recovery.
Article
Multidisciplinary Sciences
Woongjae Yoo, Jacob K. Zieba, Nora J. Foegeding, Teresa P. Torres, Catherine D. Shelton, Nicolas G. Shealy, Austin J. Byndloss, Stephanie A. Cevallos, Erik Gertz, Connor R. Tiffany, Julia D. Thomas, Yael Litvak, Henry Nguyen, Erin E. Olsan, Brian J. Bennett, Jeffrey C. Rathmell, Amy S. Major, Andreas J. Baumler, Mariana X. Byndloss
Summary: It was found that chronic exposure to a high-fat diet can increase levels of circulating trimethlamine N-oxide, a potentially harmful metabolite generated by gut microbiota.
Article
Neurosciences
Wen Yuan, Sai Ma, Juliana R. Brown, Kwanho Kim, Vanessa Murek, Lucia Trastulla, Alexander Meissner, Simona Lodato, Ashwin S. Shetty, Joshua Z. Levin, Jason D. Buenrostro, Michael J. Ziller, Paola Arlotta
Summary: This study provides an integrated epigenomic and transcriptomic analysis of mouse and marmoset neocortical neuronal classes, revealing differences in regulatory strategies during early and late stages of neuronal development. While early postmitotic neurons utilize more widely shared and evolutionarily conserved molecular regulatory programs, later stages of neuronal maturation are characterized by more brain- and neuron-specific programs with greater evolutionary divergence.
NATURE NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Byunghun So, Li Li Ji, Saba Imdad, Chounghun Kang
Summary: This study aimed to investigate the effect of voluntary wheel running (VWR) on the metabolic changes in the livers of high-fat diet (HFD)-induced NAFLD mice. LC-MS/MS was used to determine whether the tested intervention affected the protein expression profiles of the mouse livers. The results showed that VWR counteracted the adverse effects of HFD on the livers of the mice and affected the expression of proteins involved in ketogenesis, lipid metabolism, and the metabolism of drugs and xenobiotics.
Article
Multidisciplinary Sciences
Phan Tan Toi, Hyun Jae Jang, Kyeongseon Min, Sung-Phil Kim, Seung-Kyun Lee, Jongho Lee, Jeehyun Kwag, Jang-Yeon Park
Summary: Researchers have developed a two-dimensional fast line-scan approach that allows direct imaging of neuronal activity with millisecond precision, while maintaining high spatial resolution. This method was demonstrated in live mouse brain imaging, showing the sequential and laminar-specific propagation of neuronal activity along the thalamocortical pathway.
Article
Cell Biology
Aidan Schneider, Mehdi Azabou, Louis McDougall-Vigier, David F. Parks, Sahara Ensley, Kiran Bhaskaran-Nair, Tomasz Nowakowski, Eva L. Dyer, Keith B. Hengen
Summary: Cell type is a crucial factor in determining the role of neurons in a circuit. This study investigates the influence of a neuron's transcriptomic type on the timing of its activity. The researchers develop a deep-learning architecture that can analyze interevent intervals across various timescales. They find that transcriptomic cell-class information is present in the timing of single neuron activity in the brain and can be universal across different stimuli.
Article
Biology
Andres De la Rossa, Marine H. Laporte, Simone Astori, Thomas Marissal, Sylvie Montessuit, Preethi Sheshadri, Eva Ramos-Fernandez, Pablo Mendez, Abbas Khani, Charles Quairiaux, Eric B. Taylor, Jared Rutter, Jose Manuel Nunes, Alan Carleton, Michael R. Duchen, Carmen Sandi, Jean-Claude Martinou
Summary: Neuronal excitation relies on ATP from oxidative phosphorylation, and deficient oxidative phosphorylation can lead to hyperexcitability in neurons. Inhibiting GABA activity in mice with deficient mitochondrial pyruvate carrier (MPC) led to seizures and death, but providing ketone bodies restored energy and attenuated seizures. These findings provide insights into epilepsy and other neuropathologies associated with energy deficits.
Article
Neurosciences
Marija Sajic, Amy E. Rumora, Anish A. Kanhai, Giacomo Dentoni, Sharlini Varatharajah, Caroline Casey, Ryan D. R. Brown, Fabian Peters, Lucy M. Hinder, Masha G. Savelieff, Eva L. Feldman, Kenneth J. Smith
Summary: Peripheral neuropathy (PN) is a common complication in patients with prediabetes and Type 2 diabetes (T2D) and is often associated with dyslipidemia and mitochondrial dysfunction. Studies on a high-fat diet mouse model of prediabetes showed that dyslipidemia led to reduced mitochondrial membrane potential and impaired nerve impulse conduction. These results suggest that mitochondrial dysfunction may contribute to the pathogenesis of PN in prediabetes.
JOURNAL OF NEUROSCIENCE
(2021)
Correction
Multidisciplinary Sciences
Emelie Perland, Sofie V. Hellsten, Nadine Schweizer, Vasiliki Arapi, Fatemah Rezayee, Mona Bushra, Robert Fredriksson
Article
Pharmacology & Pharmacy
Gaetan Philippot, Erica Forsberg, Caroline Tahan, Henrik Viberg, Robert Fredriksson
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Genetics & Heredity
Robert Fredriksson, Smitha Sreedharan, Karin Nordenankar, Johan Alsio, Frida A. Lindberg, Ashley Hutchinson, Anders Eriksson, Sahar Roshanbin, Diana M. Ciuculete, Anica Klockars, Aniruddha Todkar, Maria G. Hagglund, Sofie Hellsten, Viktoria Hindlycke, Ake Vastermark, Ganna Shevchenko, Gaia Olivo, K. Cheng, Klas Kullander, Ali Moazzami, Jonas Bergquist, Pawel K. Olszewski, Helgi B. Schioth
Article
Physiology
Tanya Aggarwal, Sourabh Patil, Mikaela Ceder, Maher Hayder, Robert Fredriksson
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Cell Biology
Mikaela M. Ceder, Emilia Lekholm, Axel Klaesson, Rekha Tripathi, Nadine Schweizer, Lydia Weldai, Sourabh Patil, Robert Fredriksson
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell Biology
Mikaela M. Ceder, Tanya Aggarwal, Kimia Hosseini, Varun Maturi, Sourabh Patil, Emelie Perland, Michael J. Williams, Robert Fredriksson
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Emilia Lekholm, Mikaela M. Ceder, Erica C. Forsberg, Helgi B. Schioth, Robert Fredriksson
Summary: The study investigated gene expression of SV2A, SV2B and SV2C in two human neuroblastoma cell lines after differentiation, showing that different treatments affect different SV2 isoforms. Synergistic expression patterns between SV2B and CHAT, as well as SV2C and DAT, were observed, providing insights into the regulation of SV2 in neuronal cells.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2021)
Article
Genetics & Heredity
Mikaela M. Ceder, Robert Fredriksson
Summary: This study conducted a detailed mining of the SLC superfamily in humans and D. melanogaster, identifying 381 protein sequences in flies, with 55 proteins not previously identified. It found that 11 of the 65 human SLC families are not conserved in flies, while some families are highly conserved, reflecting their roles in cellular pathways.
Article
Entomology
Mikaela M. Ceder, Frida A. Lindberg, Emelie Perland, Michael J. Williams, Robert Fredriksson
Summary: The body relies on transporters to function properly, including the orphan transporter MFSD11. By studying its orthologue in fruit flies, researchers discovered its expression in the brain and its possible involvement in metabolism and locomotion. This study sheds light on the importance of characterizing orphan transporters for understanding normal conditions and diseases.
Article
Neurosciences
Jennifer Vieillard, Marina C. M. Franck, Sunniva Hartung, Jon E. T. Jakobsson, Mikaela M. Ceder, Robert E. Welsh, Malin C. Lagerstrom, Klas Kullander
Summary: Sensory-motor circuits controlling motor activity are located in the dorso-ventral interface of the spinal cord. Previous studies have associated Dmrt3-expressing neurons with locomotion coordination in horses, mice, and zebrafish. This study investigated the presynaptic inputs to spinal Dmrt3 neurons and found that they receive inputs from intrasegmental and intersegmental neurons, as well as sensory-motor control brain areas. Moreover, Dmrt3 neurons directly connect to motor neurons, suggesting their involvement in classical reflex pathways.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Neurosciences
Mikaela M. Ceder, Hannah M. Weman, Ebba Johansson, Katharina Henriksson, Kajsa A. Magnusson, Erika Roman, Malin C. Lagerstrom
Summary: The mRNA expression of the glycine receptor alpha 3 subunit (Glra3) is widely but lowly expressed in the mouse central nervous system. Male mice have higher levels of Glra3 expression in multiple brain areas compared to female mice.
Article
Biochemistry & Molecular Biology
Sofie V. Hellsten, Rekha Tripathi, Mikaela M. Ceder, Robert Fredriksson
FRONTIERS IN MOLECULAR BIOSCIENCES
(2018)