Article
Nanoscience & Nanotechnology
Zi-Yi Yang, Wen-Huei Chang, Yi-Chun Chiu, Chun-Hung Lin
Summary: Bladder cancer is a common malignancy in the urinary system, and traditional diagnostic methods often cause discomfort for patients. This study developed a rapid, low-cost, and noninvasive sensing method using gold nanomushroom (AuNM) chips for bladder cancer biomarker detection. The AuNM chips combined sandwich immunoassay and localized surface plasmon resonance (LSPR) sensing, providing a label-free platform for diagnosing and monitoring bladder cancer. The detection limits for the three biomarkers tested were 6.5, 8.3, and 7.0 pg/mL, respectively. Compared to the commercialized enzyme-linked immunosorbent assay, the AuNM chip showed better sensing performance and specificity.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Peng Zheng, Piyush Raj, Lintong Wu, Takayuki Mizutani, Miklos Szabo, William A. Hanson, Ishan Barman
Summary: The study presents a single antibody-based biosensing platform for detecting thyroid-stimulating hormone (TSH) in patient samples. The platform utilizes the Raman spectral variations induced by the interaction between a TSH antigen and a Raman molecule-conjugated TSH antibody. In conjunction with machine learning, it accurately and precisely predicts TSH concentrations in various patient samples, robust against substrate-to-substrate, intra-substrate, and day-to-day variations.
Article
Biophysics
Shih-Mo Yang, Qiang Lin, Hongbo Zhang, Ruixue Yin, Wenjun Zhang, Minchao Zhang, Yubao Cui
Summary: The study introduced a method utilizing electrokinetic effects to improve the efficiency of cytokine detection, resulting in an increased fill percentage of encoded beads and successful calculation of the concentration of four target cytokines to the pg/ml level. The application of DEP is expected to enhance the sensitivity of digital ELISA for rapid detection of multiple targets.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Thomas Mortelmans, Dimitrios Kazazis, Celestino Padeste, Philipp Berger, Xiaodan Li, Yasin Ekinci
Summary: The development of a three-dimensional nanofluidic device for rapid and multiplexed detection of viral antibodies is presented. The device is made from poly(methyl methacrylate) and utilizes nanoscale topography variations for particle immobilization and trapping. It can be used for specific and sensitive multiplexed detection of serological antibodies against different viral proteins. The device's applications can be extended to detect various diseases simultaneously.
ACS APPLIED NANO MATERIALS
(2022)
Review
Chemistry, Physical
Georgia Geka, Anastasia Kanioura, Vlassis Likodimos, Spiros Gardelis, Nikolaos Papanikolaou, Sotirios Kakabakos, Panagiota Petrou
Summary: Early diagnosis and monitoring are crucial for the effective treatment and survival of cancer patients. Immunosensors based on surface-enhanced Raman spectroscopy (SERS) have shown promise in the sensitive detection of cancer biomarkers in biological fluids. This review article focuses on the advancements made in the immunochemical determination of cancer biomarkers using SERS, including both single and multi-analyte detection. The future prospects of SERS immunosensors for cancer marker detection are also discussed.
Article
Chemistry, Multidisciplinary
Tingting Dai, Zhen Xiao, Dingying Shan, Angel Moreno, Hongquan Li, Manu Prakash, Niaz Banaei, Jianghong Rao
Summary: In this study, a fast and culture-independent method for detecting extended-spectrum beta-lactamases (ESBLs) using surface-enhanced Raman scattering (SERS) was proposed. Raman probes were used to release sulfur-based Raman active molecules in the presence of beta-lactamases, and the released thiol molecules were captured by gold nanoparticles to amplify the Raman signals. The designed probes enabled duplex detection of bacteria expressing broad-spectrum beta-lactamases or ESBLs with a detection limit of 10^3 cfu/mL in 1 hour incubation, reducing the screening time to 1.5 hours with a portable Raman microscope.
Article
Biophysics
Xiaohui Lu, Chanyu Yao, Linlin Sun, Zheng Li
Summary: MicroRNAs are reliable biomarkers for noninvasive detection of diseases, including cancers. Plasmon-enhanced biosensors are promising tools for miRNA analysis, using enhanced optical output to detect and analyze miRNA.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Li Ding, Shaohua Xu, Yueyue Huang, Yuanyuan Yao, Yueliang Wang, Lifen Chen, Yanbo Zeng, Lei Li, Zhenyu Lin, Longhua Guo
Summary: In this work, a novel multiplexed immunassay for simultaneous and fast analysis of cancer markers was developed by combining previous developed surface-enhanced electrochemiluminescence (SEECL) with a bioimaging device. A core-shell nanocomposite consisting of gold-silicon dioxide nanoparticles doped with Ru(bpy)(3)(2+) was employed as ECL label, which could significantly enhance the ECL emission of Ru(bpy)(3)(2+) due to the localized surface plasmon resonance (LSPR) of AuNPs. The proposed SEECL strategy showed high sensitivity, fast analysis, and high-throughput platform for clinical diagnosis of cancer markers in exhaled breath condensates (EBCs).
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Smruti R. Sahoo, Sandy Huey-Jen Hsu, Dev-Aur Chou, Gou-Jen Wang, Cheng-Chung Chang
Summary: The D/T-BIL ratio is related to the survival rate of COVID-19 pneumonia, as excessive bilirubin in human blood can cause liver and neurological damage. Therefore, it is imperative to detect bilirubin in a fast and label-free manner. A 3D-PHS nanochip was designed and successfully used to detect D-BIL in human blood without labels, showcasing a potential biosensing technology for health care units and point-of-care testing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Xiaoming Su, Xinyu Liu, Yangcenzi Xie, Mingyang Chen, Chao Zheng, Hong Zhong, Ming Li
Summary: Researchers have developed a paper-based biosensor using surface-enhanced Raman spectroscopy for quantitative analysis of exosomal proteins in clinical serum samples, which allows for accurate diagnosis and molecular subtyping of breast cancer.
Article
Nanoscience & Nanotechnology
Cheng Zong, Ji-Xin Cheng
Summary: Plasmon-enhanced stimulated Raman scattering (PESRS) microscopy has been developed to achieve single-molecule detection limit. The dispersive-like vibrational line shapes observed in PESRS can be explained by the interference between the local enhanced electromagnetic field induced by the plasmonic nanostructure and the molecular dipole-induced field. The PESRL and PESRG signals exhibit similar signal magnitudes but show reversed dispersive profiles under the same laser wavelength.
Article
Biochemistry & Molecular Biology
Giulia Rusciano, Angela Capaccio, Antonio Sasso, Alessandro Capo, Carlos Murillo Almuzara, Maria Staiano, Sabato D'Auria, Antonio Varriale
Summary: The development of sensitive methods for the detection of endotoxin molecules is crucial for ensuring food safety and health control. Traditional analytical methods are expensive and time-consuming, making the development of reliable biosensors highly desirable. In this study, a label-free SERS-based method was proposed, which combined plasmonic enhancement with the selectivity of an anti-lipid A antibody. The proposed technology has a low LOD and LOQ, and can potentially be extended to detect other biological macromolecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Qingnan Zhao, Hai Wang, Wenji Jiang, Huimin Gao, Sisi Wen, Xin Feng, Qiong Wu, Chengyan He, Youliang Zhu, Lianghai Hu, Bing Zhao, Wei Song
Summary: Understanding the catalytic process in an acetic acid-sodium acetate buffer environment is crucial for designing efficient nanozymes. This study investigated the lattice restructuration of Au-LaNiO3-delta nanofibers after acidification and revealed the role of carboxyl group insertion in enhancing oxidase-like activity and protecting vacancies. The strategy offers potential for constructing an efficient nanozyme-linked immunosorbent assay system.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Xinyi Liu, Meng Li, Xujiang Yu, Lisong Shen, Wanwan Li
Summary: Researchers have developed a Raman suspension array system using plasmonic microbeads as barcode substrates and Au nanoflowers as reporter carriers, enabling ultrasensitive detection of lung cancer markers. This new suspension array system has the potential to be a powerful tool for early disease screening and diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Qiong Wu, Guannan Chen, Sufang Qiu, Shangyuan Feng, Duo Lin
Summary: The study developed a target-triggered and self-calibration aptasensor based on SERS technology for sensitive and reliable detection of PSA protein, with a promising potential for accurate screening of prostate cancer in human blood samples. The method showed comparable detection accuracy, a larger dynamic detection range, a lower detection limit, and lower sample requirement compared to the clinical commonly used method.
Article
Chemistry, Multidisciplinary
Beibei Shan, Huyun Liu, Linhu Li, Yaxuan Lu, Ming Li
Summary: This study presents an all-in-one phototheranostic platform based on dpGCS NCs for combined CDT, PCT, and PTT therapies triggered by a single NIR-II laser. The platform demonstrates remarkable therapeutic efficacy in both in vitro and in vivo experiments, achieving complete inhibition of tumor growth, providing a distinctive paradigm for the development of NIR-II phototheranostic platforms.
Article
Chemistry, Analytical
Yangcenzi Xie, Yu Wen, Xiaoming Su, Chao Zheng, Ming Li
Summary: A machine learning-driven surface-enhanced Raman spectroscopy (SERS)-integrated strategy is developed for label-free detection of cellular HER2, achieving high accuracy in cell identification and classification for HER2+ breast cancer. The combination of label-free SERS detection and machine learning-driven analysis enables longitudinal monitoring of therapeutic efficacy, aiding in decision-making and management.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Beibei Shan, Yaxuan Lu, Chao Zheng, Ming Li
Summary: The influence of structural symmetry breaking in metal-semiconductor heterostructures for cancer phototheranostics is rare studied. In this study, the plasmonic properties of different structural symmetric plasmonic Au-Cu2-xSe nanocrystals (GCS NCs) were investigated. The Janus-GCS NCs demonstrated superior near-infrared II (NIR-II) plasmonic and photothermal properties, making them a promising theranostic nanoplatform for cancer therapy.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Yangcenzi Xie, Xiaoming Su, Yu Wen, Chao Zheng, Ming Li
Summary: This study reports an artificial intelligent surface-enhanced Raman spectroscopy (SERS) strategy for accurate diagnosis and assessment of breast cancer. The deep learning algorithm trained with SERS spectra achieved a 100% prediction accuracy for different breast cancer subtypes, and when combined with similarity analysis, it can evaluate the surgical outcomes.
Article
Multidisciplinary Sciences
Linhu Li, Renting Jiang, Beibei Shan, Yaxuan Lu, Chao Zheng, Ming Li
Summary: Researchers report a class of porous nanostructures composed of cubic AuAg alloy nanoshell and numerous nanopores. These porous nanostructures exhibit excellent plasmonic properties in the near-infrared II spectral range, which can be tuned by varying the pore features. Near-infrared II SERS probes created with these porous cubic AuAg nanoshells demonstrate remarkable capability for in vivo visualization of sub-millimeter microtumors in a living mouse model.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Environmental
Guangzhi Zhou, Ming Li
Summary: This study reports a new type of Cu2-xTe nanosheets that can be degraded by gluta-thione, which can effectively treat cancer through mild-photothermally enhanced chemodynamic therapy. The results show that the nanosheets have excellent plasmonic absorption and photothermal properties, and can efficiently catalyze the production of & BULL;OH in cancer cells. The study demonstrates the potential of these nanosheets as a potent CDT agent for efficient cancer treatment.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Physical
Mingyang Chen, Renata Solarska, Ming Li
Summary: Despite progress, precise evaluation of the surface-enhanced Raman scattering (SERS) substrate enhancement factor (EF) still remains unresolved. This study emphasizes the importance of probe molecules in terms of diffusion kinetics and surface coverage for accurate determination of the SERS EFs, which has not been addressed in previous research.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Nanoscience & Nanotechnology
Bin Meng, Yong Xie, Le Chen, Haitao Wang, Ming Li, Zhuxin Dong
Summary: This paper presents a convenient technical scheme for enhancing the performance of atomic force microscopy (AFM) tips in tip-enhanced Raman spectroscopy (TERS) applications. The silver decoration on homemade tips is confined to the apex of commercial tips through an AFM-controlled electrochemical reaction. The efficiency of the metal coating is ensured by the reduction of Ag+ in a highly sealed environment. Key factors affecting the process include the use of silver nitrate solution, ambient relative humidity and temperature, electric potential bias, and tip-surface distance. The silver-coated tips are then tested for TERS measurement of carbon nanotubes (CNTs), resulting in simultaneous characterization of the CNTs' morphology and chemical properties. The Raman spectra show that our plasmonic tip exhibits a local field signal increase of approximately 30-fold, and the corresponding TERS image can resolve at the single-pixel level laterally.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Xiaoming Su, Xinyu Liu, Yangcenzi Xie, Mingyang Chen, Chao Zheng, Hong Zhong, Ming Li
Summary: Researchers have developed a paper-based biosensor using surface-enhanced Raman spectroscopy for quantitative analysis of exosomal proteins in clinical serum samples, which allows for accurate diagnosis and molecular subtyping of breast cancer.
Article
Chemistry, Analytical
Xiaoming Su, Xinyu Liu, Yangcenzi Xie, Mingyang Chen, Hong Zhong, Ming Li
Summary: The widespread abuse of fentanyl has become a major global public health concern, leading to a drastic increase in overdose deaths. A recently developed freestanding surface-enhanced Raman spectroscopy (SERS) biosensor, called the FrEnSERS biosensor, has shown excellent capability in identifying and quantifying trace amounts of fentanyl in biofluids. This biosensor, composed of a reduced graphene oxide membrane decorated with hydrophobic Au nanostars, combines high SERS enhancement and analyte enrichment to achieve remarkable SERS performance. It has demonstrated sensitive and quantitative detection of fentanyl in serum and urine, with a wide dynamic range and low limits of detection. The FrEnSERS biosensor is a promising tool for forensic analysis and clinical diagnosis, providing a sensitive, cost-effective, and reliable method for rapid quantitative analysis of fentanyl in biofluids.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Haitao Wang, Huiming Zhang, Beibei Shan, Renata Solarska, Ming Li
Summary: This study numerically investigates the plasmonic and photothermal properties of Au-silica-Cu2-x Se hybrid nanoshells (Cu2-x Se HNSs) with varied core offsets. The plasmon modes in the symmetry-broken Cu2-x Se HNSs are explained by plasmon hybridization theory. The results show tunability of NIR-II thermoplasmonic properties in the Cu2-x Se HNSs through varying the core offset.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Physical
Huiming Zhang, Ting Zhu, Ming Li
Summary: In this study, the photothermal effects of Au nanoparticles (NPs) of different shapes were systematically investigated. It was found that the photothermal properties of Au NPs are closely related to their geometric shape and localized surface plasmon resonance (LSPR) wavelength. Specifically, Au NPs with sharp structural features such as Au nanorod, nanostar, and nanobipyramid exhibited exceptional light-to-heat conversion ability, indicated by their larger Joule (Jo) number.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Nanoscience & Nanotechnology
Xiaoming Su, Yangcenzi Xie, Xinyu Liu, Mingyang Chen, Chao Zheng, Hong Zhong, Ming Li
Summary: Quantitative analysis of serum exosomes was performed using surface-enhanced Raman spectroscopy (SERS) combined with a paper-based lateral flow strip (LFS) biosensor and multivariate spectral unmixing analysis. This technique allowed for absolute quantification of two different serum exosomes with a detection limit of 10(6) particles/mL. The strategy was successfully applied in the quantitative dual-plex detection of serum exosomes from breast cancer patients.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Renting Jiang, Linhu Li, Ming Li
Summary: A degradable DNAzyme biocapsule has been developed for efficient gene therapy of breast cancer. The biocapsule effectively downregulates EGR-1 and Bcl-2 protein expression, inducing apoptosis in tumor cells. This study provides a promising strategy for constructing degradable DNAzyme-based platforms for effective gene therapy of metastatic breast cancer.
Article
Chemistry, Multidisciplinary
Yangcenzi Xie, Mingyang Chen, Xinyu Liu, Xiaoming Su, Ming Li
Summary: An artificial intelligence-assisted label-free surface-enhanced Raman spectroscopy (SERS) biosensor is developed for simultaneous quantification of C-5-methylcytosine (C-5m) level and methylation ratio in DNA samples.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Alexander M. Prophet, Kritanjan Polley, Gary J. Van Berkel, David T. Limmer, Kevin R. Wilson
Summary: The oxidation kinetics of iodide by ozone at the air-water interface is studied in single microdroplets. Molecular simulations and kinetic modeling are used to understand the underlying multiphase mechanism.
Article
Chemistry, Multidisciplinary
Rong Zhang, Jiajing Lan, Fei Wang, Shumei Chen, Jian Zhang
Summary: By utilizing 1,1'-ferrocene dicarboxylic acid as a chelating and surface protection ligand, we have synthesized multi-nuclear indium oxide clusters with varying nuclear sizes, including heptanuclear and thirteen-nuclear clusters. These clusters possess labile coordination sites, allowing for structural modification and self-assembly, resulting in the formation of various cluster structures.
Article
Chemistry, Multidisciplinary
Hui Wang, Hui Liu, Mingsen Wang, Jiaheng Hou, Yongjun Li, Yuancheng Wang, Yingjie Zhao
Summary: Two supramolecular complexes were prepared using CBs and M1, and their single-crystal structures were analyzed by SCXRD. The unexpected 1:2 self-assembly structure between M1 and CB[8] was discovered for the first time. These complexes exhibit unique photophysical properties and provide valuable information about the structure and photophysical properties of supramolecular complexes.
Article
Chemistry, Multidisciplinary
Chang-Hee Lee, Sookil Park, Sanggil Kim, Ji Young Hyun, Hyun Soo Lee, Injae Shin
Summary: The epidermal growth factor receptor (EGFR) is a cell-surface glycoprotein involved in cell proliferation and tumor development. This study used a fluorescently labeled EGFR to investigate its time-dependent endocytosis in live cells and found that appended glycans affect EGFR internalization. Additionally, the study detected sialic acid residues attached to EGFR on the live cell surface using FRET-based imaging. This research provides valuable insights into the cellular functions of EGFR.
Article
Chemistry, Multidisciplinary
Subhankar Sahu, Lokesh Kumar, Sumita Das, Dipti Gupta, Ruchi Anand
Summary: This study proposes a strategy that combines organic electronics with biosensor scaffolds to create a compact device for monitoring environmental aromatic pollution. By coupling biosensing protein MopR with an organic electrochemical transistor (OECT), a sensor module capable of efficient detection of phenol was designed. Exclusive phenol detection with minimal loss of sensitivity could be achieved in complex pollutant mixtures and real environmental samples.
Review
Chemistry, Multidisciplinary
Changseok Lee, Hyung-Joon Kang, Sungwoo Hong
Summary: The formation of C-N bonds through hydroamination reactions catalyzed by nickel hydrides has been a topic of recent interest. This approach offers a way to efficiently transform a variety of alkene and alkyne substrates into compounds enriched with C-N bonds. The review provides a concise overview of the underlying reaction mechanisms and aims to stimulate further progress in NiH-catalytic techniques and catalyst design.
Article
Chemistry, Multidisciplinary
Yueci Wu, Lu-Lu Sun, Hai-Hao Han, Xiao-Peng He, Weiguo Cao, Tony D. James
Summary: Drug-induced liver injury (DILI) is a common cause of acute liver failure in the USA and Europe, but most cases can be recovered or prevented by discontinuing the offending drug. Recent research has found that peroxynitrite (ONOO-) can be used as a potential indicator for early diagnosis of DILI, and there is an urgent need to establish a method to detect and track peroxynitrite in DILI cases. In this study, a FRET-based nano fluorescent probe CD-N-I was developed, which showed high selectivity and sensitivity in detecting peroxynitrite. The probe successfully detected exogenous peroxynitrite in live cells and endogenous peroxynitrite in APAP-induced liver injury of HepG2 cells.
Article
Chemistry, Multidisciplinary
Dmitry L. Lipilin, Mikhail O. Zubkov, Mikhail D. Kosobokov, Alexander D. Dilman
Summary: This article describes a direct photocatalytic method for the thiolation of unprotected acids, which was previously challenging. By using a thionocarbonate reagent with an N-O bond, the efficient conversion of carboxylic acids to thiols is achieved.
Article
Chemistry, Multidisciplinary
Jason Malenfant, Lucille Kuster, Yohann Gagne, Kouassi Signo, Maxime Denis, Sylvain Canesi, Mathieu Frenette
Summary: Raman microscopy can reveal compound-specific vibrational fingerprints without sample preparation. The combination of efficient theoretical calculations and a user-friendly software can accurately predict peak positions and provide match scores to assist with structure determination.
Article
Chemistry, Multidisciplinary
Jayoh A. Hernandez, Paul S. Micus, Sean Alec Lois Sunga, Luca Mazzei, Stefano Ciurli, Gabriele Meloni
Summary: Essential trace metals play crucial roles in the survival and virulence of bacterial pathogens. Helicobacter pylori requires nickel for colonization and persistence in the stomach, and NixA is an essential nickel transporter in this process. This study characterizes the selectivity and electrogenic nature of NixA-mediated nickel transport.
Article
Chemistry, Multidisciplinary
Tarali Devi, Kuheli Dutta, Jennifer Deutscher, Stefan Mebs, Uwe Kuhlmann, Michael Haumann, Beatrice Cula, Holger Dau, Peter Hildebrandt, Kallol Ray
Summary: This study emphasizes the importance of subtle electronic changes and secondary interactions in the stability of biologically relevant metal-dioxygen intermediates. It also shows that the role of the chloride ligand in stabilizing the Fe-III-(OOBu)-Bu-t moiety can extend to other anions, including the thiolate ligand.
Article
Chemistry, Multidisciplinary
Jacqueline R. Santhouse, Jeremy M. G. Leung, Lillian T. Chong, W. Seth Horne
Summary: By studying the folding kinetics and mechanism of the BdpA sequence, researchers found that altering the backbone connectivity can affect protein folding. This suggests that protein mimetic chains have a significant degree of plasticity in transitioning between unfolded and folded states.
Article
Chemistry, Multidisciplinary
Divanshu Gupta, Ralf Einholz, Holger F. Bettinger
Summary: This study presents the first direct spectroscopic evidence of a cyclic seven-membered iminoborane. Compared to linear amino-iminoboranes, this cyclic iminoborane exhibits weakened bond strength and lower Lewis acidity value. The study suggests that the reduced ring strain of cyclic iminoborane prevents nitrogen fixation but allows facile (2 + 2) cycloaddition reaction with C2H4.
Article
Chemistry, Multidisciplinary
Renny Mathew, Aniruddha Mazumder, Praveen Kumar, Julie Matula, Sharmarke Mohamed, Petr Brazda, Mahesh Hariharan, Brijith Thomas
Summary: This study reveals the packing arrangement of partially disordered nitro-perylenediimide (NO2-PDI) using a synergistic approach that combines 3D ED, ssNMR, and DFT techniques. By overcoming these challenges, this methodology opens up new avenues for material characterization, driving exciting advancements in the field.
