Article
Biochemistry & Molecular Biology
Xuebing Han, Yifeng Fu, Keyu Wang, Siying Li, Chang Jiang, Shuangshuang Wang, Zheng Wang, Gang Liu, Siwang Hu
Summary: EGCG can prevent osteoporosis by regulating intestinal microbiota and serum metabolites. It inhibits the decline of bone density, protects bone microstructure, increases microbial diversity, and promotes beneficial bacteria while inhibiting pathogenic bacteria. Changes in serum metabolites mainly involve sphingolipid and glycerophospholipid metabolism pathways. These findings provide a basis and reference for the prevention and treatment of osteoporosis.
Review
Pharmacology & Pharmacy
Consolato M. Sergi
Summary: In the last few decades, there has been a global increase in the use of nutraceuticals and a demand for organic foods. One particular nutraceutical that has gained attention is epigallocatechin gallate (EGCG) found in green tea. While current evidence for the use of antioxidants in preventing and treating central nervous system diseases is limited, it has been suggested that consuming antioxidants like EGCG may benefit diseases such as Parkinson's disease. This review explores the effects of EGCG on PD through in vitro and animal models, the potential mechanisms of neuroprotection involved, and recent clinical trials in human PD. It also emphasizes the need for further investigation into nutraceuticals due to the influence of environmental factors on neurodegenerative diseases.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2022)
Article
Biophysics
Fengzhan Cui, Shuang Han, Jiangyue Wang, David Julian McClements, Xuebo Liu, Fuguo Liu
Summary: A co-encapsulation system using whey protein isolate fibrils and cellulose nano-crystals was developed to improve the stability and bioaccessibility of curcumin and epigallocatechin gallate (EGCG) in emulsion-based delivery systems. The double emulsions stabilized by the whey protein isolate fibril-cellulose nano-crystal complexes exhibited higher stability, encapsulation efficiency, and bioaccessibility for curcumin and EGCG compared to those stabilized by whey protein isolates alone.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biophysics
Omur Acet, Emrah Dikici, Burcu Onal Acet, Mehmet Odabasi, Ivan Mijakovic, Santosh Pandit
Summary: Microbial adhesion and formation of biofilms pose serious problems in various areas. Preventing microbial adhesion and biofilm formation is a promising strategy to address these issues. In this study, attaching EGCG to membranes effectively prevented bacterial colonization.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Sana Iram, Safikur Rahman, Shahid Ali, Jihoe Kim
Summary: Epigallocatechin gallate inhibits the activation of plasminogen by binding to tetranectin, showing anticancer effects on CT-26 colon cancer cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Ren Yoshitomi, Motofumi Kumazoe, Kwan-Woo Lee, Yuki Marugame, Yoshinori Fujimura, Hirofumi Tachibana
Summary: This study investigated the connection between food components and circular RNA (circRNA), as well as the regulatory effect of (-)-Epigallocatechin-3-O-gallate (EGCG) on circRNA expression in the liver. The results showed that EGCG treatment altered the expression of circRNAs in mice livers, and one upregulated circRNA (mmu_circRNA_011775) affected the expression of genes related to liver fibrosis and the cardiovascular system.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2024)
Article
Chemistry, Applied
Takashi Tanaka, Miho Yasumatsu, Mayu Hirotani, Yosuke Matsuo, Na Li, Hong-Tao Zhu, Yoshinori Saito, Kanji Ishimaru, Ying-Jun Zhang
Summary: This study investigated the reaction of Theanaphthoquinone (TNQ) with epigallocatechin-3-O-gallate (EGCg) in black tea leaves, revealing the formation of novel reaction products and providing new insights into the formation of black tea pigment thearubigins.
Article
Immunology
Yong Ma, Gang Liu, Muyang Tang, Jun Fang, Hongmei Jiang
Summary: The study demonstrated the protective effect of EGCG on LPS-induced acute injury by inhibiting the increase of pro-inflammatory factors and oxidation indicators, reshaping the intestinal microbial community, improving sphingolipid metabolism, and inhibiting harmful metabolites content.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Environmental Sciences
Gyanendra Singh, Riddhi Thaker, Anupama Sharma, Dharati Parmar
Summary: Arsenic is a common toxicant in the environment, and flavonoids such as biochanin A, phloretin, and epigallocatechin-3-gallate have been found to significantly mitigate arsenic toxicity in Swiss albino mice. The flavonoids were able to restore sperm motility, reduce arsenic concentrations in kidney and liver tissues, and modulate antioxidant markers and signaling pathways to enhance the intracellular antioxidant response system.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Seungil Park, Gyeong Han Jeong, Sung Hoon Jee, Tae Hoon Kim, Seong Bong Kim
Summary: This study reported an efficient method for dimerization of EGCG, the major bioactive constituent isolated from tea leaves, using low-temperature plasma. The contribution of ozone in the reaction process was quantified as a major factor, and the mechanism of synthesizing dimers A and B through the generation of ozone and formaldehyde during plasma treatment was investigated.
SCIENTIFIC REPORTS
(2022)
Review
Polymer Science
Mazlan Zawani, Mh Busra Fauzi
Summary: Immediate treatment of cutaneous injuries is a realistic approach to improve healing rate and minimize complications. Multifunctional biomaterials, especially those incorporating antioxidants, have potential in chronic skin wound management and tissue engineering advancements. Extensive research and studies have shown that epigallocatechin gallate (EGCG) is a potent antioxidant for wound healing purposes.
Article
Biodiversity Conservation
Xiaodong Shan, Feifei Chen, Huikang Lin, Hangjun Zhang, Yuchi Zhong, Zhiquan Liu, Yan Li
Summary: This study evaluated the protective effects of theaflavins (TFs) and epigallocatechin gallate (EGCG) against zinc oxide nanoparticles (ZnO-NPs) induced cytotoxicity. The results suggest that TFs and EGCG treatment can improve the toxicity of ZnO-NPs through antiapoptotic, antioxidant, and anti-inflammatory effects.
Article
Food Science & Technology
Zikun Li, Xiaohan Wang, Man Zhang, Hongjun He, Bin Liang, Chanchan Sun, Xiulian Li, Changjian Ji
Summary: In this study, EGCG-loaded BSA/PUL nanoparticles were successfully prepared with good stability and antioxidant activity. The formation of BPENs through self-assembly driven by hydrogen bonding ensured the desired EGCG loading efficiency. BPENs exhibited high antioxidant activity in vitro and sustained release over time.
Article
Cell Biology
Jaganmay Sarkar, Manjusri Das, Md Sariful Islam Howlader, Prateeksha Prateeksha, Derek Barthels, Hiranmoy Das
Summary: EGCG inhibits osteoclast differentiation through modulating mitophagy and ROCK-mediated pathways. This study provides important theoretical support for the development of new therapies for arthritis.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Chengyu Jiang, Li Wang, Xin Huang, Song Zhu, Chaoyang Ma, Hongxin Wang
Summary: Vinyl stearate was used in the enzymatic transesterification of EGCG to enhance its lipophilicity and antioxidant abilities, achieving a stearyl derivative with significantly improved lipid oxidation and potential applications in high-fat foods.
Review
Biochemistry & Molecular Biology
Fumie Nakashima, Takahiro Shibata, Koji Uchida
JOURNAL OF BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Kana Fujikawa, Kengo Nakahara, Nobumasa Takasugi, Tadashi Nishiya, Akihiro Ito, Koji Uchida, Takashi Uehara
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Kana Hasegawa, Keiko Kuwata, Jun Yoshitake, Sayako Shimomura, Koji Uchida, Takahiro Shibata
Summary: Inflammatory bowel diseases lead to upregulation of proteins in extracellular vesicles, particularly an increase in expression of epidermal growth factor receptor which promotes cell proliferation. The findings suggest that inflamed colon-derived extracellular vesicles promote tumor development by activating fibroblasts.
Article
Biochemistry & Molecular Biology
Keitaro Yoshioka, Haruki Yamashita, Kazunori Shimizu, Sayako Shimomura, Takahiro Shibata, Jun-ichi Miyazaki, Hiroyuki Honda
Summary: This study identified new peptides with FFAR1 agonist activity as potential new drug candidates for treating type 2 diabetes mellitus. By utilizing phage display and physiochemical property prediction methods, peptides with high FFAR1 agonist activity can be screened. These findings provide new insights for drug development in treating type 2 diabetes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Food Science & Technology
Hiroyuki Hattori, Takashi Mori, Takahiro Shibata, Masaki Kita, Tohru Mitsunaga
Summary: This study found that 6-paradol, a major vanilloid found in GOP, has anti-obesity effects by decreasing body weight gain and visceral and subcutaneous fats. It also suppresses hepatic cholesterol and triglyceride levels, and decreases gene expression related to fatty acid synthesis, lipid transportation, and adipocyte differentiation in both liver and adipose tissue. Additionally, 6-gingerol promoted phosphorylation of AMP-activated protein kinase (AMPK) while 6-paradol did not, suggesting that 6-paradol regulates obesity-related genes in an AMPK-independent manner.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Moeri Oshimo, Fumie Nakashima, Kenji Kai, Hirofumi Matsui, Takahiro Shibata, Mitsugu Akagawa
Summary: Sulfites, commonly used as preservatives and antioxidants in the food industry, have been shown to induce significant cytotoxicity in rat gastric mucosal cells through oxidative stress, forming protein carbonyls and 8-hydroxy-2'-deoxyguanosine. This indicates potential gastric tissue injury via formation of sulfite-related free radicals.
FREE RADICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Kosuke Yamaguchi, Masanori Itakura, Roma Kitazawa, Sei-Young Lim, Koji Nagata, Takahiro Shibata, Mitsugu Akagawa, Koji Uchida
Summary: The study found that polyphenols, especially catechol-type polyphenols, exhibit lysyl oxidase-like activity and mediate oxidative deamination of lysine residues in proteins. The oxidation products of lysine were identified, and it was observed that the reaction was mediated by some polyphenols, especially o-diphenolic-type polyphenols, in the presence of copper ions. The natural immunoglobulin M monoclonal antibody recognized these oxidation products, indicating a potential role in innate immune response.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Hiromasa Tanaka, Yugo Hosoi, Kenji Ishikawa, Jun Yoshitake, Takahiro Shibata, Koji Uchida, Hiroshi Hashizume, Masaaki Mizuno, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
Summary: The study identified novel chemical products in plasma-activated L-sodium lactate solution that exhibited selective cytotoxic effects on cancer cells, shedding light on the components responsible for the selective killing of cancer cells. This provides useful data for potential development of cancer treatments using plasma-activated solutions.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kazuhiro Nishiyama, Akiyuki Nishimura, Kakeru Shimoda, Tomohiro Tanaka, Yuri Kato, Takahiro Shibata, Hiroshi Tanaka, Hitoshi Kurose, Yasu-Taka Azuma, Hideshi Ihara, Yoshito Kumagai, Takaaki Akaike, Philip Eaton, Koji Uchida, Motohiro Nishida
Summary: This study identified a mechanism called redox-dependent alternative internalization (REDAI) that promotes the internalization and degradation of the purinergic P2Y(6) receptor (P2Y(6)R). The researchers found that both synthetic and natural compounds containing electrophilic isothiocyanate groups can modify P2Y(6)R and facilitate its internalization and degradation. This mechanism, mediated by cysteine modification, may also play a role in the internalization of other G protein-coupled receptors (GPCRs) and potentially limit the progression of colitis.
Article
Chemistry, Multidisciplinary
Remi Kinoshita, Ikko Kozaki, Kazunori Shimizu, Takahiro Shibata, Akihito Ochiai, Hiroyuki Honda
Summary: A method for synthesizing a free cyclic peptide library was established via peptide array synthesis, demonstrating sequence activity of cyclic peptides. Variants of oxytocin were systematically evaluated, with some showing high antagonistic activity. Notably, the Y2W variant exhibited the highest inhibitory effect.
Article
Multidisciplinary Sciences
Masanori Itakura, Kosuke Yamaguchi, Roma Kitazawa, Sei-Young Lim, Yusuke Anan, Jun Yoshitake, Takahiro Shibata, Lumi Negishi, Hikari Sugawa, Ryoji Nagai, Koji Uchida
Summary: Reducing sugars can react with proteins to form advanced glycation end products (AGEs), which are pathogenic molecules involved in inflammation and human diseases. This study identifies histone as a cell-surface receptor for AGEs and suggests that AGEs may also play a role in homeostatic response by binding to histone.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Jun Yoshitake, Mayuko Azami, Haruka Sei, Daisuke Onoshima, Kumiko Takahashi, Akiyoshi Hirayama, Koji Uchida, Yoshinobu Baba, Takahiro Shibata
Summary: Extracellular vesicles are nanoscale lipid bilayer vesicles released by almost all cell types. They play an important role in cell-cell communication and have potential applications in disease diagnosis and drug delivery. In this study, a size-exclusion chromatography column device was developed for rapid and effective isolation of extracellular vesicles.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Fumie Nakashima, Juan A. Gimenez-Bastida, Paula B. Luis, Sai H. Presley, Robert E. Boer, Manuel Chiusa, Takahiro Shibata, Gary A. Sulikowski, Ambra Pozzi, Claus Schneider
Summary: Consecutive oxygenation of arachidonic acid by 5-lipoxygenase and cyclooxygenase-2 produces hemiketal ei-cosanoids, HKE2 and HKD2, which promote angiogenesis by inducing endothelial cell tubulogenesis. This study identifies VEGFR2 as a mediator of HKE2-induced angiogenesis in vitro and in vivo. HKE2 treatment increases the phosphorylation of VEGFR2 and downstream kinases ERK and Akt, while HKE2-mediated effects are blocked by the VEGFR2 inhibitor vatalanib. These findings suggest a potential therapeutic strategy utilizing drugs targeting the arachidonic acid pathway for antiangiogenic therapy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Takeshi Katayoshi, Yuri Kusano, Takahiro Shibata, Koji Uchida, Kentaro Tsuji-Naito
Summary: The study found that low-molecular-weight whey proteins can accelerate dermal collagen production through the TβR/Smad pathway, with components such as beta-lactoglobulin and alpha-lactalbumin playing a role in this process, highlighting their therapeutic potential in wound healing.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Suguru Ariyoshi, Yusuke Imazu, Ryuji Ohguri, Ryo Katsuta, Arata Yajima, Takahiro Shibata, Makoto Ojika
Summary: This study identified two biosynthetic intermediates, 11- and 16-hydroxyphytols, for the mating hormone alpha 2 in the plant pathogen Phytophthora. The results suggest that alpha 2 is possibly synthesized by two cytochrome P450 oxygenases via two hydroxyphytol intermediates in A2 hyphae and secreted outside.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)