Article
Microbiology
Intraporn Srisangthong, Chadtida Sangseedum, Netnapa Chaichanit, Komwit Surachat, Naraid Suanyuk, Pimonsri Mittraparp-arthorn
Summary: In this study, a V. campbellii-specific bacteriophage OPA17 was isolated, characterized, and found to effectively control V. campbellii infection and had lytic activity against other bacteria. It belongs to the Siphoviridae family, can survive in artificial seawater for two months, and effectively destroys V. campbellii biofilms. Therefore, OPA17 has the potential to be a candidate for phage therapy against V. campbellii.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Jinchang Liang, Jiwen Liu, Xiaolei Wang, Hao Sun, Yulin Zhang, Feng Ju, Fabiano Thompson, Xiao-Hua Zhang
Summary: This study compared the genomes of five Vibrio campbellii strains isolated from different depths of the Mariana and Yap Trenches and identified genomic characteristics that facilitate survival in the deep sea. Pelagic strains showed genome streamlining, while deep-sea strains had more genes involved in amino acid metabolism, cell wall biogenesis, and ion transport and metabolism. Deep-sea strains also had specific antibiotic resistance and substrate degradation genes. In contrast, pelagic strains had the ability for assimilatory nitrate reduction.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
C. O. Lomeli-Ortega, A. J. Martinez-Sandez, D. R. Barajas-Sandoval, A. G. Reyes, F. Magallon-Barajas, B. Veyrand-Quiros, L. Gannon, C. Harrison, S. Michniewski, A. Millard, E. Quiroz-Guzman
Summary: This study describes a lytic phage vB_Vc_SrVc9 against a pathogenic Vibrio campbellii strain and demonstrates its potential to eliminate pathogens without impacting the natural microbiota. This novel phage is stable, has a short latent period, and can eliminate multiple strains, making it a promising strategy to reduce mortality caused by pathogenic bacteria in farmed organisms.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Immunology
Xiaoting Zheng, Biao Han, Vikash Kumar, Adam F. Feyaerts, Patrick Van Dijck, Peter Bossier
Summary: The study found that essential oils from Melaleuca alternifolia (0.0008%) and Litsea citrata (0.002%) significantly increased the survival of gnotobiotic brine shrimp Artemia franciscana challenged with Vibrio campbellii. These essential oils may modulate virulence factor production and immune-related gene expression, potentially becoming part of an intervention strategy to control vibriosis in aquaculture settings.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Sujeet Kumar, Chandra Bhushan Kumar, Vidya Rajendran, Nishawlini Abishaw, P. S. Shyne Anand, S. Kannapan, Viswas K. Nagaleekar, K. K. Vijayan, S. V. Alavandi
Summary: Luminescent vibriosis is a significant bacterial disease in shrimp hatcheries, and the presence of V. campbellii with various virulence markers, especially ToxR, plays a crucial role in regulating pathogenicity pathways. Specific amino acid sequence variants and the presence of ToxR have a significant impact on the pathogenicity of V. campbellii.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Shanshan Zhang, Qian Yang, Tom Defoirdt
Summary: Bacterial diseases are a major problem in the aquaculture industry and have led to the spread of antibiotic resistance. This study identified a class of indole analogues that have antimicrobial and antivirulence properties against Vibrio campbellii. These compounds showed a high protective effect on brine shrimp even at low concentrations, and they inhibited swimming motility and biofilm formation of V. campbellii.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Chelsea A. Simpson, Blake D. Petersen, Nicholas W. Haas, Logan J. Geyman, Aimee H. Lee, Ram Podicheti, Robert Pepin, Laura C. Brown, Douglas B. Rusch, Michael P. Manzella, Kai Papenfort, Julia C. van Kessel
Summary: This study revealed significant differences in quorum sensing systems between Vibrio campbellii DS40M4 and Vibrio campbellii BB120, as well as observed variations in LuxM/LuxN protein conservation within and between Vibrio species. Additionally, phenotypic differences between DS40M4 and BB120 were noted, with DS40M4 exhibiting stronger interbacterial cell killing and BB120 forming more robust biofilms and emitting bioluminescence. Wild isolates should be examined to gain a broader understanding of bacterial diversity in the marine ecosystem.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Blake D. Petersen, Michael S. Liu, Ram Podicheti, Albert Ying-Po Yang, Chelsea A. Simpson, Chris Hemmerich, Douglas B. Rusch, Julia C. van Kessel
Summary: The study using Vibrio campbellii as a model organism revealed a multi-tiered regulatory hierarchy of flagellar gene regulation in this bacterium, as well as critical differences from other Vibrio species.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wipa Suginta, Surapoj Sanram, Anuwat Aunkham, Mathias Winterhalter, Albert Schulte
Summary: The study investigated the pH dependency of interactions between natural substrate chitohexaose and deacetylated chitosan hexaose with chitoporin using black lipid membrane electrophysiology, revealing the crucial role of C2 substitution for chitoporin entry. Deacetylation led to loss of distinctive structural features, impacting pore access of chitosugars.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Maura Cesaria, Matteo Calcagnile, Pietro Alifano, Rosella Cataldo
Summary: Biofilms formed by Vibrio campbellii strains play a crucial role in genetic and adaptive resistance to antibiotics and disease control strategies. This study investigates mature high-coverage biofilm formations through digital image processing, revealing specific short-range orientational correlation and coherent growth pathways. These findings cannot be visualized or analyzed by traditional methods and have implications for drug and material screening.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Anuwat Aunkham, Wipa Suginta
Summary: VhChiP is a sugar-specific porin found on the outer membrane of Vibrio campbellii, with three prominent extracellular loops playing specific roles in maintaining structure, regulating binding affinity for sugar substrates, and stabilizing the open state of the channel.
BIOPHYSICAL JOURNAL
(2021)
Article
Multidisciplinary Sciences
Angela Weigert Munoz, Elisabeth Hoyer, Kilian Schumacher, Marianne Grognot, Katja M. Taute, Stephan M. Hacker, Stephan A. Sieber, Kirsten Jung
Summary: Catecholamines serve as signaling molecules between animals and bacteria, promoting bacterial growth under iron limitation and enhancing colony expansion on soft agar. These hormones affect bacterial chemotactic control, with CheW identified as a major target for EPI and NE.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Julia Schwarz, Sophie Brameyer, Elisabeth Hoyer, Kirsten Jung
Summary: The marine pathogen Vibrio campbellii activates the Cad system to resist acid threats, with the transcriptional activator AphB regulating the expression of CadC. In vitro studies show that AphB binds more tightly to DNA under low pH, allowing for tight control of the Cad system activity.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Do-Won Park, Song-Hee Kim, Jong-Hyun Park
Summary: Analysis of prophage distribution and phage characteristics based on the genome of Lactobacillus plantarum derived from kimchi revealed different types of intact prophages belonging to the Siphoviridae family. These prophages co-evolved with their hosts and could only be induced by DNA damage, while they had negligible impact on host viability during kimchi fermentation.
Article
Microbiology
Taiyeebahvi Nuidate, Aphiwat Kuaphiriyakul, Komwit Surachat, Pimonsri Mittraparp-arthorn
Summary: The study revealed the presence of a prophage named HY01 in the emerging shrimp pathogen Vibrio campbellii, describing its genome sequence and characteristics. The phage HY01 showed similarities with encoded proteins of various Gram-negative bacteria, indicating horizontal gene exchange between bacterial families has influenced its evolution.
Article
Biochemistry & Molecular Biology
Miriam Pfab, Pavel Kielkowski, Ralph Krafczyk, Wolfram Volkwein, Stephan A. Sieber, Juergen Lassak, Kirsten Jung
Summary: The study identified the kinetics of EpmA catalyzing the post-translational modification of K34 in EF-P, showing that natural (R)-beta-lysylation was more effective than synthetic modifications. This work not only provides new insights into the function of EF-P, but also introduces a new approach for post-translationally modifying proteins using EpmA.
Article
Biochemistry & Molecular Biology
Benedikt Graf von Armansperg, Franziska Koller, Nicola Gericke, Michael Hellwig, Pravin Kumar Ankush Jagtap, Ralf Heermann, Janosch Hennig, Thomas Henle, Juergen Lassak
Summary: Thermally processed foods contain compounds such as fructoselysine, which can be utilized by gut microbiota as a nutrient source. The metabolism of fructoselysine in E. coli is tightly controlled at the transcriptional level, with the global regulator CRP (CAP) and the alternative sigma factor sigma 32 (RpoH) playing key roles in promoter activation. Additionally, a transcriptional regulator FrlR has been identified as a specific repressor for fructoselysine-6-phosphate, highlighting the complex interplay between global and substrate-specific regulation for efficient utilization of unusual substrates in the human gut environment.
MOLECULAR MICROBIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Juergen Lassak, Andreas Schlundt
BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Ralph Krafczyk, Fei Qi, Alina Sieber, Judith Mehler, Kirsten Jung, Dmitrij Frishman, Juergen Lassak
Summary: Translation speed of mRNA is influenced by the amino acid being incorporated into the nascent chain, with proline causing slower peptide bond formation and ribosome stalling. The codon usage and tRNA selection also play a role in regulating pausing strength, affecting protein copy numbers. Proline codon usage and prolyl-tRNA abundance impact mRNA translation rates of polyproline-motifs in bacteria, providing insights into sequence-specific translation regulation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nikolay Manavski, Sebastien Mathieu, Margarita Rojas, Louis-Valentin Meteignier, Andreas Brachmann, Alice Barkan, Kamel Hammani
Summary: An artificial PPR protein, built from consensus PPR motifs, demonstrated in vivo RNA binding specificity and substitution capabilities for natural PPR proteins, showing potential for mimicking native PPR proteins in serving as physical barriers against exoribonucleases.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Gerasimos Gkoutselis, Stephan Rohrbach, Janno Harjes, Martin Obst, Andreas Brachmann, Marcus A. Horn, Gerhard Rambold
Summary: Microplastics serve as a carrier for diverse fungal organisms, including potential pathogens, attracting distinctive fungal communities. Studies have shown that plastic waste harbors a variety of fungal species, some of which may pose risks to human health.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Seomun Kwon, Oliver Rupp, Andreas Brachmann, Christopher Frederik Blum, Anton Kraege, Alexander Goesmann, Michael Feldbruegge
Summary: Research has shown that EV-associated mRNAs secreted by the maize smut pathogen are resistant to degradation by RNases and contain mRNAs related to metabolism and virulence. Known effectors and proteins linked to virulence are also present in EVs, and some of these mRNAs are upregulated during infection.
Review
Biochemistry & Molecular Biology
Juergen Lassak, Alina Sieber, Michael Hellwig
Summary: Among the 22 proteinogenic amino acids, lysine stands out due to its diverse post-translational modifications, which provide a wide range of possibilities to influence protein function and cellular physiology. Lysine derivatives also serve as a metabolic reservoir, conferring selective advantages to organisms that can utilize them.
BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Duncan B. Crosbie, Maryam Mahmoudi, Viviane Radl, Andreas Brachmann, Michael Schloter, Eric Kemen, Macarena Marin
Summary: Nodule microbiota, primarily consisting of symbiotic nitrogen-fixing rhizobia, are influenced by other non-rhizobial bacteria, which may impact root-nodule symbiosis in a plant host-specific manner.
Article
Biotechnology & Applied Microbiology
Nazzareno Dominelli, Heidi Yoko Jaeger, Angela Langer, Andreas Brachmann, Ralf Heermann
Summary: This study investigates the phenotypic heterogeneity in the bacterium Photorhabdus luminescens. Through genome sequencing and comparative analysis, the researchers found that the observed phenotypic differences are not caused by genetic mutations or rearrangements.
ANNALS OF MICROBIOLOGY
(2022)
Article
Microbiology
Mandy Messal, Maria Vivas, Martin Kemler, Dominik Begerow, Andreas Brachmann, Frederick Witfeld, Sanushka Naidoo, Bernard Slippers
Summary: This study aims to understand the correlation between insect-associated gall formation and the diversity of foliar fungal communities in surrounding healthy leaf tissue, as well as the co-occurrence patterns among the members of the fungal community. The results suggest that the composition and diversity of fungal communities are influenced by the severity of insect infestations.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Fede Berckx, Cyndi Mae Bandong, Daniel Wibberg, Jorn Kalinowski, Joost Willemse, Andreas Brachmann, Jessica Simbahan, Katharina Pawlowski
Summary: A novel species of Streptomyces, named Streptomyces coriariae sp. nov., was isolated from the root nodules of a Coriaria intermedia plant along Halsema Highway in Luzon, Philippines. The strain CMB-FB showed high sequence similarity to several known Streptomyces species based on 16S rRNA gene sequence analysis. The isolate also displayed characteristic features such as major menaquinones, polar lipid profile, diagnostic diamino acid of the peptidoglycan, and major fatty acids.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Mycology
Marco Alexandre Guerreiro, Stephan Kambach, Raphael Stoll, Andreas Brachmann, Juergen Senker, Dominik Begerow, Derek Persoh
Summary: In forest ecosystems, decomposition plays a crucial role in carbon and nutrient cycling. This study aimed to characterize chemical shifts during decomposition and link them to changes in decomposer fungal activity. By using proton nuclear magnetic resonance spectroscopy, the researchers analyzed the chemical composition of freshly fallen autumn leaves and the corresponding leaf litter after 1 year of decomposition. They also assessed the composition and transcriptional activity of fungal communities in the litter samples. The study found that chemical composition of the leaves changed with decomposition, and the fungal communities were functionally redundant in terms of litter degrading enzymes. Fungal genera Mycena and Chalara were found to correlate with the transcription of litter-degrading enzymes in the 1-year-old litter.
MYCOLOGICAL PROGRESS
(2023)
Article
Forestry
Tanay Bose, Maria Vivas, Bernard Slippers, Jolanda Roux, Martin Kemler, Dominik Begerow, Frederick Witfeld, Andreas Brachmann, Steven Dovey, Michael J. Wingfield
Summary: Retaining post-harvest residues in South African plantation soil can improve the diversity of beneficial soil fungi. This research demonstrates that retaining post-harvest residues is important for improving tree and soil health by enhancing the diversity of beneficial fungi in plantation soils.
FOREST ECOLOGY AND MANAGEMENT
(2023)
Article
Parasitology
Robert E. Rollins, Gabriele Margos, Andreas Brachmann, Stefan Krebs, Alexia Mouchet, Niels J. Dingemanse, Abdelkarim Laatamna, Nassiba Reghaissia, Volker Fingerle, Dirk Metzler, Noemie S. Becker, Lidia Chitimia-Dobler
Summary: Ticks are important vectors of human and animal pathogens, and understanding their taxonomy is crucial. This study utilized molecular tools to confirm the identification of Ixodes inopinatus ticks collected from Germany, and found that genetically they represented Ixodes ricinus, rather than I. inopinatus. The results also suggested that I. inopinatus may be geographically isolated and raised questions about its existence in central Europe. The study highlights the power of genomic data in answering questions regarding tick taxonomy.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2023)
