Article
Virology
Ramon D. Perez, Elena E. Gorbonova, Erich R. Mackow
Summary: ANDV infects pericytes and increases PMEC permeability, potentially contributing to HPS. Through the modulation of VEGF secretion, infected pericytes indirectly affect PMEC permeability.
Article
Immunology
Grant C. Paulsen, Robert Frenck Jr, Kay M. Tomashek, Rodolfo M. Alarcon, Elizabeth Hensel, Ashley Lowe, Rebecca L. Brocato, Steve A. Kwilas, Matthew D. Josleyn, Jay W. Hooper
Summary: This study evaluated the safety and immunogenicity of an ANDV DNA vaccine, which showed that the vaccine was safe and induced a robust and durable immune response.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Rene Lopez, Mauricio Espinoza, Jeronimo Graf, Gregory Mertz, Marcela Ferres, Mario Calvo, Cecilia Vial, Pablo A. Vial
Summary: Proteinuria is a common finding in patients with HCPS caused by Andes virus and is associated with a higher mortality rate.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Rheumatology
Nicole Yager, Suzanne Cole, Alicia Lledo Lara, Ash Maroof, Frank Penkava, Julian C. Knight, Paul Bowness, Hussein Al-Mossawi
Summary: By using mass cytometry combined with transcriptomic analysis, this study identified significant differences in myeloid cell populations between psoriatic arthritis (PsA) synovial fluid (SF) and blood, with expansion of intermediate monocytes, macrophages, and dendritic cells in SF. Myeloid cells, particularly classical monocytes, intermediate monocytes, and macrophages, were found to produce proinflammatory mediators like osteopontin and CCL2 spontaneously in PsA SF, while minimal cytokine production was detected in T cells. Gene expression analysis revealed upregulation of osteopontin and CCL2 genes in PsA monocytes/macrophages, which were also elevated at the protein level in PsA SF.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Mahendra Jadhao, Chun-Lin Chen, Wangta Liu, Dhanashri Deshmukh, Wei-Ting Liao, Jeff Yi-Fu Chen, Ritesh Urade, Eing-Mei Tsai, Sheng-Kai Hsu, Li-Fang Wang, Chien-Chih Chiu
Summary: This study investigated the impact of phthalate exposure on angiogenesis in triple-negative breast cancer cells. The findings revealed that exposure to phthalates induced angiogenesis in breast cancer cells. Additionally, the overexpression of the glycoprotein endoglin (ENG) was found to be associated with tumor development and growth, and knocking down ENG inhibited phthalate-induced angiogenesis. Targeting ENG could potentially serve as an alternative treatment for controlling angiogenesis, limiting metastasis, and inhibiting cancer progression.
Article
Cell Biology
Eva Mittler, Alexandra Serris, Emma S. Esterman, Catalina Florez, Laura C. Polanco, Cecilia M. O'Brien, Megan M. Slough, Janne Tynell, Remigius Groning, Yan Sun, Dafna M. Abelson, Anna Z. Wec, Denise Haslwanter, Markus Keller, Chunyan Ye, Russel R. Bakken, Rohit K. Jangra, John M. Dye, Clas Ahlm, C. Garrett Rappazzo, Rainer G. Ulrich, Larry Zeitlin, James C. Geoghegan, Steven B. Bradfute, Simone Sidoli, Mattias N. E. Forsell, Tomas Strandin, Felix A. Rey, Andrew S. Herber, Laura M. Walker, Kartik Chandran, Pablo Guardado-Calvo
Summary: We isolated a monoclonal broadly neutralizing antibody from a Puumala virus-experienced human donor and determined its structure when bound to the viral fusion complex. The antibody recognizes conserved sequences in the fusion loop of the Gc protein and the main chain of variable Gn sequences, thereby locking the Gn/Gc heterodimer in its prefusion conformation. We optimized the antibody variant to overcome its limited potency against the divergent Andes virus at endosomal acidic pH, making it a promising therapeutic candidate for pan-hantavirus treatment.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Immunology
Misa Pavletic, Misa Korva, Natasa Knap, Petra Bogovic, Lara Lusa, Klemen Strle, Mirijam Nahtigal Klevisar, Tomaz Vovko, Janez Tomazic, Stanka Lotric-Furlan, Franc Strle, Tatjana Avsic-Zupanc
Summary: Vaccinated breakthrough TBE patients display a global inflammatory state with elevated levels of inflammatory cytokines in serum and CSF, indicating localization of immune responses in the CNS. Compared to unvaccinated patients, these breakthrough cases exhibit significantly higher levels of inflammatory mediators and sustained systemic immune activation. Neutrophil-derived inflammation and increased VEGF-A expression could contribute to CNS immune response localization and BBB disruption in breakthrough TBE patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Biology
Andrew T. Jacob, Benjamin M. Ziegler, Stefania M. Farha, Lyla R. Vivian, Cora A. Zilinski, Alexis R. Armstrong, Andrew J. Burdette, Dia C. Beachboard, Christopher C. Stobart
Summary: This review provides updates on Sin Nombre virus (SNV) and other New World hantaviruses, including their history, biology, ecology, and disease in humans. It highlights the importance of studying and preventing emerging viruses in North America.
Article
Biochemistry & Molecular Biology
Christiana Magkrioti, Georgia Antonopoulou, Dionysios Fanidis, Vaia Pliaka, Theodore Sakellaropoulos, Leonidas G. Alexopoulos, Christoph Ullmer, Vassilis Aidinis
Summary: This study investigated the response of human proximal tubular epithelial cells to LPA and other pathological stimuli, identifying signaling pathways and inflammatory factors that may play a role in the pathogenesis of CKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Eva Mittler, Anna Z. Wec, Janne Tynell, Pablo Guardado-Calvo, Julia Wigren-Bystrom, Laura C. Polanco, Cecilia M. O'Brien, Megan M. Slough, Dafna M. Abelson, Alexandra Serris, Mrunal Sakharkar, Gerard Pehau-Arnaudet, Russell R. Bakken, James C. Geoghegan, Rohit K. Jangra, Markus Keller, Larry Zeitlin, Olli Vapalahti, Rainer G. Ulrich, Zachary A. Bornholdt, Clas Ahlm, Felix A. Rey, John M. Dye, Steven B. Bradfute, Tomas Strandin, Andrew S. Herbert, Mattias N. E. Forsell, Laura M. Walker, Kartik Chandran
Summary: This study isolated neutralizing antibodies against tetrameric Gn/Gc glycoprotein spikes from PUUV-experienced donors, and identified a dominant class of antibodies that recognize the capping loop of Gn. One of these antibodies, ADI-42898, blocked the cell entry of multiple HCPS- and HFRS-associated hantaviruses, and provided protection against highly virulent HCPS-causing ANDV and HFRS-causing PUUV in Syrian hamsters and bank voles, respectively. ADI-42898 is a potential therapeutic candidate for both HCPS and HFRS, and its mode of recognition informs the development of broad-spectrum hantavirus vaccines.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Entomology
Angie Gisseth Ladino Penuela, Juan Pablo Botero, Luiz Felipe Lima da Silveira
Summary: Through the revision of two Andean-endemic firefly species, their distant relationships were revealed. The study first reported the interaction between these firefly species and Andean-endemic flowers, suggesting their similarities may be attributed to mimicry rings. Additionally, the research found that Pseudolychnuris and Alychnus may act as occasional pollinators of Andean-endemic plants.
Article
Cell Biology
Jacqueline M. Ratter, Julia I. P. van Heck, Hanne M. M. Rooijackers, Henry J. Jansen, Pleun C. M. van Poppel, Cees J. Tack, Rinke Stienstra
Summary: The study found that insulin acutely activates the metabolism of human monocytes and induces a shift toward a more proinflammatory phenotype, which may contribute to chronic inflammation in patients with diabetes.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Qian Xie, Tao Wen, Aiyun Yang, Xue Zhang, Bo Chen, Jie Meng, Jian Liu, Ning Gu, Haiyan Xu
Summary: The study compared the biological effects of gamma-Fe2O3 nanoparticles and Gd-DTPA on the kidneys of healthy mice, showing that gamma-Fe2O3 nanoparticles induced weaker proinflammatory effects and better renal safety compared to Gd-DTPA.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Immunology
Seyyed Mostafa Arabi, Leila Sadat Bahrami, Mahsa MalekAhmadi, Mahla Chambari, Narges Milkarizi, Alexander N. Orekhov, Amirhossein Sahebkar
Summary: This systematic review and meta-analysis evaluates the impact of combination therapy with statins and ezetimibe on proinflammatory cytokines. The results indicate that the combination therapy does not significantly reduce IL-1 beta, TNF-alpha, and MCP-1, but shows significant reductions in IL-6 and IFN-gamma levels. Furthermore, the reduction in IL-6 is particularly significant among individuals aged >= 60 years and the Asian population.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Immunology
Ying Li, Xiaolin Wang, Hanxue Yang, Yanlin Li, Jingang Gui, Yonghua Cui
Summary: This study conducted a meta-analysis to investigate immune dysfunction in pediatric TS patients, focusing on proinflammatory cytokines and T-cell changes. The results showed elevated levels of specific proinflammatory cytokines and disproportionate changes in T-cell subpopulations in TS patients. Increased IL-6 levels and a reduced number of T helper cells were identified with small to large effect sizes, while increased TNF-alpha levels had a large effect size. These findings indicate a close association between peripheral immune activation and TS, but further exploration is needed to understand the direct and meaningful interaction between peripheral immune status and microglial activation in the central nervous system in TS patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Kimia T. Maleki, Marina Garcia, Ayelen Iglesias, Daniel Alonso, Matias Ciancaglini, Ulf Hammar, Hans-Gustaf Ljunggren, Pablo Schierloh, Valeria P. Martinez, Jonas Klingstrom
JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Virology
Lies Laenen, Valentijn Vergote, Charles H. Calisher, Boris Klempa, Jonas Klingstroem, Jens H. Kuhn, Piet Maes
Article
Cell Biology
Caries Sola-Riera, Shawon Gupta, Kimia T. Maleki, Patricia Gonzalez-Rodriguez, Dale Saidi, Christine L. Zimmer, Sindhu Vangeti, Laura Rivino, Yee-Sin Leo, David Chien Lye, Paul A. MacAry, Clas Ahlm, Anna Smed-Sorensen, Bertrand Joseph, Niklas K. Bjorkstrom, Hans-Gustaf Ljunggren, Jonas Klingstrom
Article
Virology
Wanda Christ, Janne Tynell, Jonas Klingstrom
JOURNAL OF VIROLOGY
(2020)
Article
Microbiology
Carles Sola-Riera, Marina Garcia, Hans-Gustaf Ljunggren, Jonas Klingstrom
Article
Multidisciplinary Sciences
Egle Kvedaraite, Laura Hertwig, Indranil Sinha, Andrea Ponzetta, Ida Hed Myrberg, Magda Lourda, Majda Dzidic, Mira Akber, Jonas Klingstrom, Elin Folkesson, Jagadeeswara Rao Muvva, Puran Chen, Sara Gredmark-Russ, Susanna Brighenti, Anna Norrby-Teglund, Lars Eriksson, Olav Rooyackers, Soo Aleman, Kristoffer Stralin, Hans-Gustaf Ljunggren, Florent Ginhoux, Niklas K. Bjorkstrom, Jan-Inge Henter, Mattias Svensson
Summary: This study found that COVID-19 patients experience a redistribution of monocyte subsets towards intermediate monocytes and a general decrease in circulating dendritic cells. Severe disease is associated with the appearance of monocytic myeloid-derived suppressor cell-like cells and a higher frequency of pre-DC2. The phenotypic alterations in mononuclear phagocytes are cell-lineage-specific and linked to the response against SARS-CoV-2 or COVID-19 severity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Maria Eugenia Dieterle, Carles Sola-Riera, Chunyan Ye, Samuel M. Goodfellow, Eva Mittler, Ezgi Kasikci, Steven B. Bradfute, Jonas Klingstrom, Rohit K. Jangra, Kartik Chandran
Summary: Research using CRISPR/Cas9 technology to knockout candidate hantavirus receptors in human endothelial cells found that the loss of beta 3 integrin, beta 1 integrin, and/or DAF had little or no effect on hantavirus entry, while loss of protocadherin-1 significantly reduced virus entry and infection. Major host molecules necessary for hantavirus entry by PCDH1-independent mechanisms are yet to be discovered.
Article
Infectious Diseases
Yong Jin Yi, Minsun Kang, Won-Keun Kim, Kyungmin Huh, Jonas Klingstrom, Jin-Won Song, Jaehun Jung
Summary: Patients with HFRS have a significantly higher risk of developing haematologic and solid organ malignancies compared to the general population, particularly in terms of leukemia and non-Hodgkin lymphoma.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Sebastian Havervall, August Jernbom Falk, Jonas Klingstrom, Henry Ng, Nina Greilert-Norin, Lena Gabrielsson, Ann-Christin Salomonsson, Eva Isaksson, Ann-Sofie Rudberg, Cecilia Hellstrom, Eni Andersson, Jennie Olofsson, Lovisa Skoglund, Jamil Yousef, Elisa Pin, Wanda Christ, Mikaela Olausson, My Hedhammar, Hanna Tegel, Sara Mangsbo, Mia Phillipson, Anna Manberg, Sophia Hober, Peter Nilsson, Charlotte Thalin
Summary: Current serological assays for SARS-CoV-2 generate inconsistent results and the longitudinal characteristics of antibodies targeting different antigens after asymptomatic or mild COVID-19 have not been established. This cohort study involving healthcare workers found that antibodies against the SARS-CoV-2 spike antigen remained detectable in 96% of participants at least four months after infection, even if they had no or mild symptoms. The study also confirmed virus neutralization capacity in 91% of participants at least four months after infection.
Article
Multidisciplinary Sciences
Chengzi Kaku, Elizabeth R. Champney, Johan Normark, Marina Garcia, Carl E. Johnson, Clas Ahlm, Wanda Christ, Mrunal Sakharkar, Margaret E. Ackerman, Jonas Klingstrom, Mattias N. E. Forsell, Laura M. Walker
Summary: Heterologous prime-boost immunization strategies have the potential to enhance the efficacy of COVID-19 vaccines. A study found that heterologous vaccination induced stronger neutralizing antibody and memory B cell responses, as well as better efficacy against variant strains. The study also revealed that heterologous vaccination can enhance the quality of B cell responses.
Article
Immunology
Ulrika Marking, Sebastian Havervall, Nina Greilert-Norin, Henry Ng, Kim Blom, Peter Nilsson, Mia Phillipson, Sophia Hober, Charlotta Nilsson, Sara Mangsbo, Wanda Christ, Jonas Klingstrom, Max Gordon, Mikael Aberg, Charlotte Thalin
Summary: Heterologous primary SARS-CoV-2 vaccination with ChAd and BNT elicits a stronger initial immune response compared to homologous vaccination with ChAd, particularly in terms of antibody response. However, although the differences in humoral responses remain over 6 months, the difference in SARS-CoV-2 specific T cell responses are no longer significant three months after vaccination.
Letter
Medicine, General & Internal
Sebastian Havervall, Ulrika Marking, Julia Svensson, Nina Greilert-Norin, Philip Bacchus, Peter Nilsson, Sophia Hober, Max Gordon, Kim Blom, Jonas Klingstrom, Mikael Aberg, Anna Smed-Sorensen, Charlotte Thalin
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Editorial Material
Virology
Jens H. Kuhn, Steven B. Bradfute, Charles H. Calisher, Boris Klempa, Jonas Klingstrom, Lies Laenen, Gustavo Palacios, Connie S. Schmaljohn, Nicole D. Tischler, Piet Maes
Summary: The official classification of viruses by the ICTV requires complete or nearly complete genome sequences to be deposited in GenBank. This requirement is fairly new, resulting in fragmented or absent genomic sequence information for many classified viruses. This poses challenges for taxon-wide phylogenetic analyses, especially for viruses with segmented genomes. To address this issue for Hantaviridae, the community is urged to provide additional sequence information for incompletely sequenced classified viruses by mid-June 2023 to prevent possible declassification.
Letter
Health Care Sciences & Services
Kim Blom, Oscar Bladh, Sebastian Havervall, Ulrika Marking, Nina Greilert Norin, Jessica J. Alm, Jonas Klingstrom, Charlotte Thalin
LANCET REGIONAL HEALTH-EUROPE
(2023)
Article
Virology
Wanda Christ, Jonas Klingstrom, Janne Tynell
Summary: The integrated stress response (ISR) is activated in response to SARS-CoV-2 infection, triggering translational arrest and inhibiting stress granule formation. However, the stress-responsive transcription factors ATF4 and CHOP are not induced. Different SARS-CoV-2 variants show variant-specific differences in ISR activation, suggesting potential impacts on pathogenesis and treatment strategies.