Article
Biochemical Research Methods
Jack Taylor, Kevin Patio, Gabriele De Rubis, Micheal B. Morris, Christian Evenhuis, Michael Johnson, Mary Bebawy
Summary: This study examined the role of alpha II-spectrin in vesiculation of malignant and non-malignant plasma membrane, using high resolution confocal microscopy to evaluate its subcellular distribution. The results showed distinct differences in cortical spectrin localization between malignant and non-malignant cells at rest, suggesting that the redistribution of cortical alpha II-spectrin supports plasma membrane-derived EV biogenesis in malignant cells.
Article
Multidisciplinary Sciences
Arkadiusz Miazek, Michal Zalas, Joanna Skrzymowska, Bryan A. Bogin, Krzysztof Grzymajlo, Tomasz M. Goszczynski, Zachary A. Levine, Jon S. Morrow, Michael C. Stankewich
Summary: The study identified a strain of mice with a single spectrin mutation that affects its affinity to calmodulin and increases sensitivity to calpain proteolysis, leading to neurological abnormalities and lethality. This highlights the importance of substrate-level regulation of spectrin cleavage for neuronal integrity and suggests a potential role of spectrin damage in various neurodegenerative disorders.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Pedro Monteiro, David Remy, Eline Lemerle, Fiona Routet, Anne-Sophie Mace, Chloe Guedj, Benoit Ladoux, Stephane Vassilopoulos, Christophe Lamaze, Philippe Chavrier
Summary: This study reveals the unique distribution of caveolae and invadosomes along collagen fibrils, and the reciprocal interplay between these two structures in coordinating cell adhesion and extracellular matrix remodeling.
NATURE CELL BIOLOGY
(2023)
Article
Biology
Sharon R. Stevens, Colleen M. Longley, Yuki Ogawa, Lindsay H. Teliska, Anithachristy S. Arumanayagam, Supna Nair, Juan A. Oses-Prieto, Alma L. Burlingame, Matthew D. Cykowski, Mingshan Xue, Matthew N. Rasband
Summary: AnkR is highly enriched in Pv(+) fast-spiking interneurons in mice and humans, and is associated with cytoskeletal proteins, cell adhesion molecules (CAMs), and perineuronal nets (PNNs). Loss of AnkR leads to disruption of PNNs, decreases in anxiety-like behaviors, and changes in the excitability and firing properties of Pv(+) fast-spiking interneurons, which is accompanied by a reduction in Kv3.1b K+ channels. AnkR plays a crucial role in organizing ion channels, CAMs, and PNNs in interneurons and at nodes of Ranvier.
Article
Cell Biology
Huawei Zhang, Sunlong Li, Jiajie Lu, Jie Jin, Gaosheng Zhu, Libo Wang, Yingzhao Yan, Linjie He, Ben Wang, Xiangyang Wang, Huachen Yu
Summary: The study showed that alpha-Cyperone effectively inhibited inflammation and extracellular matrix degradation through multiple pathways, making it a potential drug for treating osteoarthritis.
Correction
Multidisciplinary Sciences
Arkadiusz Miazek, Michal Zalas, Joanna Skrzymowska, Bryan A. Bogin, Krzysztof Grzymajlo, Tomasz M. Goszczynski, Zachary A. Levine, Jon S. Morrow, Michael C. Stankewich
Summary: An amendment to this paper has been published and can be accessed via a link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Consuelo Ibar, Krishna Chinthalapudi, Sarah M. Heissler, Kenneth D. Irvine
Summary: Scientists have found that Drosophila beta-heavy spectrin, encoded by the karst gene, regulates Hippo signaling through the Jub biomechanical pathway by influencing cytoskeletal tension. They also discovered that it interacts with myosin, providing new insights into the mechanisms of cell shape change.
Article
Multidisciplinary Sciences
Joao Vasco Ferreira, Ana da Rosa Soares, Jose Ramalho, Catarina Maximo Carvalho, Maria Helena Cardoso, Petra Pintado, Ana Sofia Carvalho, Hans Christian Beck, Rune Matthiesen, Monica Zuzarte, Henrique Girao, Guillaume van Niel, Paulo Pereira
Summary: This article describes a mechanism in which proteins containing a KFERQ motif are loaded into a specific type of exosomes dependent on the membrane protein LAMP2A. This mechanism facilitates the transfer of hypoxia signaling to normoxic cells and provides a method for interorgan transfer of exosomes by tagging proteins with KFERQ-like sequences.
Article
Dentistry, Oral Surgery & Medicine
Hongrong Zhang, Weihong Wang, Yemei Qian, Lanlan Zhang
Summary: This study aimed to investigate the effects of key pathogenic genes and extracellular matrix (ECM) on osteogenic differentiation injaw ameloblastoma (AB). Essential genes for AB were identified through whole-exome sequencing and microarray datasets analysis, and the expression of key genes and their encoded proteins in AB tissues was explored. ABdECM tissues were generated and the osteogenic development of PDLSCs was mimicked to simulate the AB tumor microenvironment. The study found that abnormal ECM proteins encoded by COL4A2, COL1A2, FBN1, and HPSE genes can cause disturbance in the ECM environment of AB and promote bone resorption.
Article
Physiology
Reut Shainer, Vardit Kram, Tina M. Kilts, Li Li, Andrew D. Doyle, Inbal Shainer, Daniel Martin, Carl G. Simon, Jinyang Zeng-Brouwers, Liliana Schaefer, Marian F. Young, Genomics and Computational Biol Core
Summary: Biglycan is a small proteoglycan found in the extracellular matrix and plays an important role in bone development. Our study reveals that biglycan is involved in osteoblast maturation during embryonic development, affecting bone integrity and strength. Deletion of the biglycan gene reduces the inflammatory response after fracture, leading to impaired periosteal expansion and callus formation. Additionally, biglycan is crucial for the cartilage phase preceding bone formation, as its absence leads to accelerated bone development with high levels of osteopontin, which negatively impacts bone structural integrity.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Elke Pach, Maike Kuemper, Julia E. Fromme, Jan Zamek, Fabian Metzen, Manuel Koch, Cornelia Mauch, Paola Zigrino
Summary: The deletion of MMP14 in dermal fibroblasts leads to the deposition of an extracellular matrix that inhibits melanoma growth, with collagen XIV identified as a key regulator in melanoma progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Xiaoyi Liu, Ryan M. M. Burke, Janet K. K. Lighthouse, Cameron D. D. Baker, Ronald A. A. Dirkx Jr, Brian Kang, Yashoswini Chakraborty, Deanne M. M. Mickelsen, Jennifer J. J. Twardowski, Stephano S. S. Mello, John M. M. Ashton, Eric M. M. Small
Summary: This study reveals a mechanism regulating cardiac fibroblast accumulation and ECM secretion, orchestrated in part by p53-dependent cell cycle control that governs the timing and extent of fibrosis in left ventricular pressure overload.
CIRCULATION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Andy Chan, Sophia Ma, Bret J. Pearson, Danny Chan
Summary: The extracellular matrix plays a crucial role in the maintenance, regeneration, and differentiation of stem cells within their niche. By studying the collagen gene family in the freshwater planarian Schmidtea mediterranea, researchers have identified a key gene, col4-1, which influences the proliferation of neoblasts. Furthermore, evidence suggests that an interaction between type IV collagen, the discoidin domain receptor, and neuregulin-7 regulate the balance of symmetric and asymmetric division of neoblasts through the NRG-7/EGFR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Yaru Guo, Siqin Ma, Mingming Xu, Yan Wei, Xuehui Zhang, Ying Huang, Ying He, Boon Chin Heng, Lili Chen, Xuliang Deng
Summary: Angiogenesis is crucial in tissue engineering and regenerative medicine, and the interplay between ECs and the ECM plays a significant role in tip cell specification. HtrA3 protein is shown to be involved in this process, promoting tip cell morphogenesis through ECM degradation and integrin beta 1-related signaling pathways. This study suggests that HtrA3 could potentially serve as a therapeutic agent for enhancing angiogenesis and targeting pathological angiogenesis.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Cell Biology
Qunshan Lu, Peilai Liu, Zhuang Miao, Desu Luo, Songlin Li, Mei Lu
Summary: Our study demonstrates that SIRT1 can inhibit EGFR ubiquitination by down-regulating PTEN, thereby preventing extracellular matrix degradation and activating chondrocyte autophagy. Knockdown of SIRT1 accelerates the progression of osteoarthritis in the pathological process.
CELL DEATH DISCOVERY
(2022)
Article
Cell Biology
Justyna Lisowska, Claudia Jasmin Roedel, Sandra Manet, Yekaterina A. Miroshnikova, Cyril Boyault, Emmanuelle Planus, Richard De Mets, Hsiao-Hui Lee, Olivier Destaing, Hichem Mertani, Gwenola Boulday, Elisabeth Tournier-Lasserve, Martial Balland, Salim Abdelilah-Seyfried, Corinne Albiges-Rizo, Eva Faurobert
JOURNAL OF CELL SCIENCE
(2018)
Article
Multidisciplinary Sciences
Carlos Pardo-Pastor, Fanny Rubio-Moscardo, Marina Vogel-Gonzalez, Selma A. Serra, Alexandros Afthinos, Sanela Mrkonjic, Olivier Destaing, Juan F. Abenza, Jose M. Fernandez-Fernandez, Xavier Trepat, Corinne Albiges-Rizo, Konstantinos Konstantopoulos, Miguel A. Valverde
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Medicine, Research & Experimental
Cecile Otten, Jessica Knox, Gwenola Boulday, Mathias Eymery, Marta Haniszewski, Martin Neuenschwander, Silke Radetzki, Ingo Vogt, Kristina Haehn, Coralie De Luca, Cecile Cardoso, Sabri Hamad, Carla Igual Gil, Peter Roy, Corinne Albiges-Rizo, Eva Faurobert, Jens P. von Kries, Monica Campillos, Elisabeth Tournier-Lasserve, W. Brent Derry, Salim Abdelilah-Seyfried
EMBO MOLECULAR MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Asma Boudria, Cherine Abou Faycal, Tao Jia, Stephanie Gout, Michelle Keramidas, Chloe Didier, Nicolas Lemaitre, Sandra Manet, Jean-Luc Coll, Anne-Claire Toffart, Denis Moro-Sibilot, Corinne Albiges-Rizo, Veronique Josserand, Eva Faurobert, Christian Brambilla, Elisabeth Brambilla, Sylvie Gazzeri, Beatrice Eymin
Article
Cell Biology
Richard De Mets, Irene Wang, Martial Balland, Christiane Oddou, Philippe Moreau, Bertrand Fourcade, Corinne Albiges-Rizo, Antoine Delon, Olivier Destaing
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Multidisciplinary Sciences
K. Hennig, I. Wang, P. Moreau, L. Valon, S. DeBeco, M. Coppey, Y. A. Miroshnikova, C. Albiges-Rizo, C. Favard, R. Voituriez, M. Balland
Article
Hematology
Abdoul K. Dembele, Claudine Lapoumeroulie, Mor Diaw, Oumarou Tessougue, Lucile Offredo, Dapa A. Diallo, Saliou Diop, Jacques Elion, Yves Colin-Aronovicz, Pierre-Louis Tharaux, Xavier Jouven, Marc Romana, Brigitte Ranque, Caroline Le Van Kim
Summary: This study analyzed blood cell-derived microparticles in stable SCD patients living in sub-Saharan Africa, revealing associations between microparticles and complications such as retinopathy, priapism, and leg ulcers. Furthermore, the study suggests a weak relevance of the hyper-hemolysis versus hyper-viscous paradigm in Africa.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Article
Hematology
Mickael Marin, Camille Roussel, Michael Dussiot, Papa A. Ndour, Olivier Hermine, Yves Colin, Alan Gray, Matt Landrigan, Caroline Le Van Kim, Pierre A. Buffet, Pascal Amireault
Summary: Rejuvenation of red blood cells restores intracellular ATP levels, reduces phosphatidylserine (PS) exposure, and decreases the proportion of morphologically altered red blood cells accumulated during storage. Rejuvenation also improves the filterability of red blood cells and prevents their adhesion to endothelial cells.
Article
Surgery
Johannes Klaeger, Farsad Eskandary, Georg A. Boehmig, Nicolas Kozakowski, Alexander Kainz, Yves Colin Aronovicz, Jean-Pierre Cartron, Stephan Segerer, Heinz Regele
Summary: The study revealed that upregulation of DARC protein is associated with antibody-mediated rejection in renal allografts, but is not specific as a diagnostic marker, and does not correlate with graft survival rates.
TRANSPLANT INTERNATIONAL
(2021)
Article
Engineering, Biomedical
Adria Sales, Valia Khodr, Paul Machillot, Line Chaar, Laure Fourel, Amaris Guevara-Garcia, Elisa Migliorini, Corinne Albiges-Rizo, Catherine Picart
Summary: By studying the presentation of matrix-bound BMPs on films of controlled stiffness, it was found that different BMP members have specific effects on cell adhesion and differentiation of skeletal progenitors. This response involves certain BMP receptors and beta chain integrins and is dependent on the stiffness of the substrate.
Article
Cell Biology
Alexander Kyumurkov, Anne-Pascale Bouin, Mathieu Boissan, Sandra Manet, Francesco Baschieri, Mathilde Proponnet-Guerault, Martial Balland, Olivier Destaing, Myriam Regent-Kloeckner, Claire Calmel, Alice Nicolas, Francois Waharte, Philippe Chavrier, Guillaume Montagnac, Emmanuelle Planus, Corinne Albiges-Rizo
Summary: ICAP-1 regulates integrin endocytosis by interacting with NME2, a key regulator of dynamin-dependent clathrin-coated pits fission. The control of clathrin-mediated integrin endocytosis by an integrin inhibitor is a unique mechanism to finely tune physical forces at focal adhesions. The impact of internalization on cellular mechanics and its mechanism remains elusive.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ziad Ibrahim, Tao Wang, Olivier Destaing, Nicola Salvi, Naghmeh Hoghoughi, Clovis Chabert, Alexandra Rusu, Jinjun Gao, Leonardo Feletto, Nicolas Reynoird, Thomas Schalch, Yingming Zhao, Martin Blackledge, Saadi Khochbin, Daniel Panne
Summary: This article investigates how acetylation reading and writing enable the binding of the oncogenic fusion protein BRD4-NUT with the acetylase p300, resulting in self-organizing reactions that enable long-range intra- and interchromosomal interactions.
NATURE COMMUNICATIONS
(2022)