Article
Chemistry, Applied
Carolina Zulian Boeira, Maria Augusta de Carvalho Silvello, Rafael Diaz Remedi, Ana Carla Penteado Feltrin, Lucielen Oliveira Santos, Jaqueline Garda-Buffon
Summary: This study aimed to evaluate the mitigation of NIV in alcoholic fermentation using MF. The GSH content and PO activity were increased with the presence of NIV, and after 48 hours of fermentation, the alcohol profile was altered. By the end of the fermentation, NIV was mitigated by 56.5%. This method shows promise in reducing NIV contamination, although the mycotoxin can affect the chemical characteristics of the final product.
Article
Plant Sciences
Jiaojie Zhao, Weishuai Bi, Shuqing Zhao, Jun Su, Mengyu Li, Lisong Ma, Xiumei Yu, Xiaodong Wang
Summary: The study demonstrates that overexpression of TaLTP3 in wheat enhances resistance against leaf rust pathogen infection through the activation of pathogenesis-related protein TaPR1a and multiple plant hormone pathways. The physical association between TaLTP3 and TaPR1a in the apoplast leads to increased accumulation of reactive oxygen species during plant defense responses, providing new insights into the functional roles of PR proteins in plant defense mechanisms.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sina Bader, Julia Wilmers, Teona Ontikatze, Violetta Ritter, Verena Jendrossek, Justine Rudner
Summary: Tumor hypoxia is a major driver of resistance to chemotherapy and radiotherapy. The pro-oxidative drug dihydroartemisinin (DHA) has shown to efficiently target both normoxic and hypoxic cancer cells. The mechanism behind DHA-mediated cytotoxicity in hypoxia is different from that in normoxia, with a complex interplay of apoptosis, autophagy, and cell death pathways.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Carolyn Chee, Chris E. Shannon, Aisling Burns, Anna L. Selby, Daniel Wilkinson, Kenneth Smith, Paul L. Greenhaff, Francis B. Stephens
Summary: Increasing muscle total carnitine content in older men can enhance fat oxidation and intramyocellular lipid utilization during exercise. However, this intervention did not lead to improvements in resting insulin-stimulated whole-body or skeletal muscle glucose disposal. Further research is needed to explore the potential metabolic benefits of carnitine supplementation in older adults with impaired lipid turnover.
Article
Microbiology
Xudong Tian, Guillaume Manat, Elise Gasiorowski, Rodolphe Auger, Samia Hicham, Dominique Mengin-Lecreulx, Ivo Gomperts Boneca, Thierry Touze
Summary: This study reveals that Escherichia coli has intrinsic resistance to polymyxin B through the simultaneous activation of the PhoPQ and PmrAB regulatory systems under intricate environmental cues. Among various LPS modifications, only EptA and ArnT-dependent decorations are required for polymyxin B resistance, while compromising the innate resistance of E. coli to deoxycholate. The balance between EptA- and LpxT-dependent decorations on lipid A is critical for the lifestyle of E. coli.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Nicholas de Jager, Varsa Shukla, Anna Koprivova, Martin Lycka, Lorina Bilalli, Yanrong You, Juergen Zeier, Stanislav Kopriva, Daniela Ristova
Summary: Sulfur is an essential mineral nutrient for plant growth and development. Its content in leaves is tightly connected with sulfate uptake, concentration of sulfate and phosphate anions, and synthesis of glucosinolates and glutathione. Sulfur homeostasis in Arabidopsis thaliana is regulated by biotic and abiotic stressors, leading to large variation in sulfur content among different accessions.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Jie Shen, Xi Wang, Huifang Song, Mingyang Wang, Tianzeng Niu, Haiying Lei, Cheng Qin, Ake Liu
Summary: This study investigated the response of sunflowers to drought stress and rehydration through physiological measurements and transcriptomics. The results showed that drought increased ROS accumulation in sunflowers, but rehydration led to a decrease in ROS levels. The analysis of differentially expressed genes revealed the involvement of genes related to plant hormone signal transduction and secondary metabolite biosynthesis in sunflower drought resistance.
Article
Oncology
Jaewang Lee, Jong-Lyel Roh
Summary: Cancer cells reprogram their metabolism to a mesenchymal state, making them resistant to therapy but susceptible to ferroptosis induction. Ferroptosis is a regulated cell death process based on iron-dependent lipid peroxidation. Glutathione peroxidase 4 (GPX4) is a key regulator of ferroptosis and its synthesis and expression can be controlled through various levels of regulation. Targeting GPX4 may be a promising strategy for inducing ferroptosis and killing therapy-resistant cancer cells.
Article
Plant Sciences
Zeeshan Z. Banday, Nicolas M. Cecchini, DeQuantarius J. Speed, Allison T. Scott, Claire Parent, Ciara T. Hu, Rachael C. Filzen, Elinam Agbo, Jean T. Greenberg
Summary: Hybrid proline-rich proteins play broad and distinct roles in immunity, development, and growth responses to microbes. They are localized at plastid membranes and other sites, and regulate the transport of signaling molecules.
Article
Microbiology
Yajing Song, Zhen Zhou, Jing Gu, Junmei Yang, Jiaoyu Deng
Summary: This study revealed that the resistance of E. coli to trimethoprim (TMP) is associated with the deletion of genes related to glutathione metabolism, leading to activation of drug resistance mechanisms when reducing the periplasmic glutathione content in E. coli.
MICROBIOLOGY SPECTRUM
(2021)
Article
Plant Sciences
Fali Li, Ying Ma, Yuying Yi, Meijuan Ren, Luqi Li, Ying Chen, Ao Li, Sirui Han, Haiqing Tang, Honglei Jia, Xiaofeng Wang, Jisheng Li
Summary: A study reveals that nitric oxide (NO) increases cellulose content by S-nitrosylation of cellulose synthase 1 (CESA1) and CESA9, thereby promoting cell wall biosynthesis in plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agronomy
Yuqing Li, Usman Ali, Zhouxiao Cao, Chenghao Zeng, Mengying Xiao, Fang Wei, Xuan Yao, Liang Guo, Shaoping Lu
Summary: The BnaA09.FAX1 gene plays an important role in improving seed oil accumulation and plant growth in Brassica napus, while the function of BnaFAX1 may be compensated by other homologous genes of BnaFAX1 and other BnaFAX genes in the mutants.
MOLECULAR BREEDING
(2022)
Article
Reproductive Biology
Kelli F. Malott, Samantha Reshel, Laura Ortiz, Ulrike Luderer
Summary: Glutathione (GSH) deficiency leads to increased oxidative stress, reduced mitochondrial membrane potential, and aberrant lipid storage in oocytes, resulting in decreased oocyte competence.
BIOLOGY OF REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Francisco M. Gamez-Arjona, Stefania Vitale, Aline Voxeur, Susanne Dora, Sascha Mueller, Gloria Sancho-Andres, Juan Carlos Montesinos, Antonio Di Pietro, Clara Sanchez-Rodriguez
Summary: This study reveals the role of cellulase activity in fungal pathogen colonization. The researchers found that while the pathogen Fo does not require complete cellulase arsenal to infect the host, reducing cellulase activity has a severe negative impact on its saprophytic growth and conidia production during the final stages of the infection cycle.
Article
Microbiology
Zi Wang, Ju-Hong Chen, Ling-Shuai Wang, Juan Ding, Ming-Wen Zhao, Rui Liu
Summary: This study revealed, for the first time, the mechanism through which protein phosphatase regulates Ganoderma lingzhi polysaccharide biosynthesis. Silencing the GlPP2C1 gene increased the content of polysaccharides and enhanced cell wall resistance in Ganoderma lingzhi.
Editorial Material
Plant Sciences
Amey Redkar, Mugdha Sabale, Antonio Di Pietro
Summary: Plant vascular diseases are tissue-specific systemic infections caused by bacterial and fungal pathogens adapted to thrive in xylem vessels. Recent research reveals that the switch from non-vascular to vascular pathogenesis in the phytopathogenic bacterium Xanthomonas is determined by a single gene encoding a plant cell wall-degrading hydrolase.
TRENDS IN PLANT SCIENCE
(2021)
Article
Food Science & Technology
Guixia Hao, Susan McCormick, Helene Tiley, Thomas Usgaard
Summary: The study demonstrates that transgenic Arabidopsis expressing FgTri101 can detoxify trichothecenes and excrete the acetylated toxins out of plant cells, providing plant protection.
Article
Cell Biology
Laurine M. Gilles, Andrea R. M. Calhau, Veronica La Padula, Nathanael M. A. Jacquier, Claire Lionnet, Jean-Pierre Martinant, Peter M. Rogowsky, Thomas Widiez
Summary: Phospholipases play a crucial role in plant reproduction, especially in the development of male gametes and sexual reproduction. The mutation of phospholipase NOT-LIKE-DAD affects the attachment to the pollen endo-plasma membrane through lipid anchoring and electrostatic interactions, where phosphatidylinositol-4,5-bisphosphate is enriched, defining a specific polar domain critical for plant reproduction and gamete formation.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Plant Sciences
William T. Hay, Susan P. McCormick, Martha M. Vaughan
Summary: This study reveals that atmospheric CO2 concentration and temperature have significant impacts on Fusarium graminearum infection in wheat and corn, with lower CO2 and cooler temperatures leading to higher toxin accumulation, while warmer temperatures suppress pathogen growth and toxin accumulation.
Article
Food Science & Technology
Hina Shanakhat, Susan P. McCormick, Mark Busman, Joseph O. Rich, Matthew G. Bakker
Summary: This study characterized the transformation products of deoxynivalenol using a combination of fungal laccase and a chemical mediator. The results showed that alcohol groups on deoxynivalenol were oxidized to ketones, and the chemical mediator formed a covalent bond with the molecule. Understanding these chemical modifications is crucial for effective remediation of contaminated grain.
Article
Biotechnology & Applied Microbiology
Rosa E. Cardoza, Susan P. McCormick, Inmaculada Izquierdo-Bueno, Natalia Martinez-Reyes, Laura Lindo, Daren W. Brown, Isidro G. Collado, Robert H. Proctor, Santiago Gutierrez
Summary: The current study identified aspinolide biosynthetic genes (asp1 and asp2) in Trichoderma arundinaceum and found that they are part of a gene cluster shared with Aspergillus ochraceus. Deletion of these genes confirmed their essential role in aspinolide production. Furthermore, a mutation in asp2 led to increased inhibition of fungal growth and coincided with increased HA production, suggesting a trade-off between the two metabolites.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Yannick Fierlej, Nathanael M. A. Jacquier, Loic Guille, Jeremy Just, Emilie Montes, Christelle Richard, Jeanne Loue-Manifel, Nathalie Depege-Fargeix, Antoine Gaillard, Thomas Widiez, Peter M. Rogowsky
Summary: In this study, a maize protoplast system coupled with NGS analysis and a novel bioinformatics pipeline was established to evaluate the efficiency of CRISPR/Cas9 tools. The results showed a correlation between mutation types observed in protoplasts and those obtained after stable transformation. Additionally, factors influencing genome editing efficiency were identified, and phenotypic analysis was conducted on base-edited maize plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Rosa E. Cardoza, Susan P. McCormick, Laura Lindo, Sara Mayo-Prieto, David Gonzalez-Cazon, Natalia Martinez-Reyes, Guzman Carro-Huerga, Alvaro Rodriguez-Gonzalez, Robert H. Proctor, Pedro A. Casquero, Santiago Gutierrez
Summary: This article explores the importance of farnesol in Trichoderma physiology and ecology. The study reveals that the overexpression of dpp1, the gene responsible for farnesol production, leads to changes in squalene and ergosterol levels but does not affect antifungal activity. Additionally, it is found that a dpp1-overexpressing transformant acts as a sensitizing agent, up-regulating plant defense genes in the presence of a fungal plant pathogen. The toxic effects of farnesol on Trichoderma and plants are also examined.
Article
Plant Sciences
Jin Hoon Jang, Hae Seong Seo, Thomas Widiez, Ok Ran Lee
Summary: Producing haploid plants that inherit chromosomes from only one parent can greatly improve breeding efficiency. In monocots, a mutated pollen-specific phospholipase A can induce in vivo haploid induction, but a functional orthologous gene has not been identified in dicots. In Arabidopsis, loss-of-function of gynoecium-expressed phospholipase AII triggers maternal haploid plants, suggesting that altered localization of PIN1 in female organs may play a role in maternal haploid induction.
Letter
Plant Sciences
Nathanael M. A. Jacquier, Andrea R. M. Calhau, Yannick Fierlej, Jean-Pierre Martinant, Peter M. Rogowsky, Laurine M. Gilles, Thomas Widiez
Article
Plant Sciences
Gerit Bethke, Yadong Huang, Goetz Hensel, Shane Heinen, Chaochih Liu, Skylar R. Wyant, Xin Li, Maureen B. Quin, Susan Mccormick, Peter L. Morrell, Yanhong Dong, Jochen Kumlehn, Silvio Salvi, Franz Berthiller, Gary J. Muehlbauer
Summary: Through studying 496 barley accessions, it was found that the UGT13248 gene plays a role in resistance to Fusarium head blight (FHB) by converting DON to D3G, a nontoxic form; two mutant alleles (T368I and H369Y) were identified that increase FHB severity and reduce D3G production.
Article
Plant Sciences
William T. Hay, James A. Anderson, David F. Garvin, Susan P. Mccormick, Mark Busman, Martha M. Vaughan
Summary: This study demonstrates that wheat with the Fhb1 quantitative trait locus (QTL) provides reliable resistance to Fusarium head blight (FHB) at elevated CO2 levels. However, the presence of the Fhb1 QTL in more FHB-resistant wheat leads to increased disease spread and mycotoxin contamination.
Article
Plant Sciences
Guixia Hao, Nicholas A. Rhoades, Susan McCormick
Summary: This study found that chitin and laminarin can induce different responses in wheat, enhancing the production of reactive oxygen species (ROS). This study provides novel information for enhancing plant resistance against fungal infections.
Meeting Abstract
Cell Biology
Nathanael Jacquier, Andrea Calhau, Chloe Plagnard, Laurine Gilles, Peter M. Rogowsky, Jean-Pierre Martinant, Thomas Widiez
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2022)
Article
Agronomy
Jose M. Segui-Simarro, Nathanael M. A. Jacquier, Thomas Widiez
Summary: Doubled haploids (DH) have become a powerful tool used by breeding companies to produce pure lines for hybrid seed production. The biotechnological revolution has led to the development of multiple approaches for producing haploids and DHs in various species, offering flexibility and reliability in the process.
DOUBLED HAPLOID TECHNOLOGY, VOL. 1: General Topics, Alliaceae, Cereals
(2021)