Article
Biochemistry & Molecular Biology
Dong Gun Kim, Hak Sung Kim, Yoonjoo Choi, Razvan Costin Stan
Summary: Fever is a common symptom of infectious diseases. At mild fever temperatures, the binding affinity between SARS-CoV-2 and neutralizing antibodies improves, and the antibodies may exert mechanical effects on the spike protein, hindering viral infectivity.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Molly Kozminsky, Thomas R. Carey, Lydia L. Sohn
Summary: DNA-directed patterning offers versatility in immobilizing and segregating lipid-based nanoparticles for subsequent analysis, providing a wide variety of custom assays for the characterization of any lipid-based nanoparticle with high control and precision.
Article
Immunology
Nathalie Vanderheijden, Annelies Stevaert, Jiexiong Xie, Xiaolei Ren, Cyril Barbezange, Sam Noppen, Isabelle Desombere, Bruno Verhasselt, Peter Geldhof, Nick Vereecke, Veerle Stroobants, Dayoung Oh, Merijn Vanhee, Lieve M. J. Naesens, Hans J. Nauwynck
Summary: Broadly protective therapeutic antibodies are urgently needed to combat SARS-CoV-2 variants. Researchers have found that antibodies formed during coronavirus infection can target multiple coronaviruses. These antibodies can bind to both alpha- and betacoronaviruses, but their neutralizing activity against SARS-CoV-2 is weak. However, they may still play a role in influencing the severity of coronavirus infection and disease through Fc-mediated effector functions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Fortunato Ferrara, M. Frank Erasmus, Sara D'Angelo, Camila Leal-Lopes, Andre A. Teixeira, Alok Choudhary, William Honnen, David Calianese, Deli Huang, Linghan Peng, James E. Voss, David Nemazee, Dennis R. Burton, Abraham Pinter, Andrew R. M. Bradbury
Summary: This study demonstrates that highly potent human neutralizing antibodies against the SARS-CoV-2 spike protein can be generated directly from a semisynthetic naive antibody library. These antibodies have comparable affinities, developability properties, and neutralization activities to those from immune sources, providing a potential alternative to traditional immunization methods.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Wen-Ching Lin, Hao-Cheng Tang, Han Ying Wang, Chia-Yi Kao, You-Chiun Chang, Athena Hsu Li, Shi-Bing Yang, Kurt Yun Mou
Summary: This study introduces a unique mutagenesis method called the reverse Kunkel method, which combines the advantages of user-defined mutagenesis and random mutagenesis. By coupling with phage display and yeast display selections, the method successfully generates improved antibodies with enhanced affinity and immunostaining performance.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Romany J. McLure, Sheena E. Radford, David J. Brockwell
Summary: Directed evolution is a powerful tool for engineering new functions in biomolecules and gaining insights into protein behavior. It accelerates the process of natural evolution through multiple rounds of gene diversification and selection.
TRENDS IN CHEMISTRY
(2022)
Article
Immunology
Ramin Sedaghat Herati, David A. Knorr, Laura A. Vella, Luisa Victoria Silva, Lakshmi Chilukuri, Sokratis A. Apostolidis, Alexander C. Huang, Alexander Muselman, Sasikanth Manne, Oliva Kuthuru, Ryan P. Staupe, Sharon A. Adamski, Senthil Kannan, Raj K. Kurupati, Hildegund C. J. Ertl, Jeffrey L. Wong, Stylianos Bournazos, Suzanne McGettigan, Lynn M. Schuchter, Ritesh R. Kotecha, Samuel A. Funt, Martin H. Voss, Robert J. Motzer, Chung-Han Lee, Dean F. Bajorin, Tara C. Mitchell, Jeffrey Ravetch, E. John Wherry
Summary: Anti-PD-1 immunotherapy has dynamic effects on noncancer immune responses, enhancing the Tfh-B cell axis response to influenza vaccination and potentially affecting immune predisposition.
Article
Biochemistry & Molecular Biology
Gennady Verkhivker, Steve Agajanian, Ryan Kassab, Keerthi Krishnan
Summary: By integrating conformational dynamics, binding energetics, and allosteric interactions, this study explores the mechanism of ultrapotent antibodies neutralizing the Omicron variant, suggesting that the patterns of escape mutants may be determined by multiple factors including protein stability, binding affinity, and long-range interactions.
Article
Biochemistry & Molecular Biology
Monica L. Fernandez-Quintero, Clarissa A. Seidler, Patrick K. Quoika, Klaus R. Liedl
Summary: Sharks and other cartilaginous fish are phylogenetically oldest living organisms with antibodies as part of their adaptive immune system. The research on V-NAR antibodies reveals a substantial rigidification upon affinity maturation, leading to a decrease in flexibility and an increase in stabilizing interactions. Additionally, the V-NAR antibodies follow the concept of conformational selection, with a strong population shift towards the native binding pose upon affinity maturation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Immunology
Damiano Buratto, Abhishek Saxena, Qun Ji, Guang Yang, Sergio Pantano, Francesco Zonta
Summary: SARS-CoV-2 infects humans by binding to the hACE2 receptor with its spike glycoprotein, and mutations can impact the binding affinity, leading to the emergence of variants. It is crucial to rapidly assess the characteristics of these variants to understand their potential effects and evolutionary pressures.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Ali Zhang, Hannah D. Stacey, Michael R. D'Agostino, Yona Tugg, Art Marzok, Matthew S. Miller
Summary: Neutralizing antibodies play a crucial role in protecting against SARS-CoV-2 infection and have been suggested as a useful indicator for vaccine trials and population surveys. Besides directly neutralizing the virus, antibodies can also interact with immune cells and complement through their Fc domains. The outcome of these interactions depends on various factors, including antibody isotype-Fc receptor combinations and post-translational modifications. Understanding the roles of Fc-dependent antibody effector functions in SARS-CoV-2 infection has implications for the development of future vaccines and therapeutics.
NATURE REVIEWS IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Susanne Ghandili, Martin Schoenlein, Christian Wiessner, Heiko Becher, Marc Luetgehetmann, Thomas Theo Brehm, Julian Schulze zur Wiesch, Carsten Bokemeyer, Marianne Sinn, Katja C. Weisel, Lisa B. Leypoldt
Summary: This study reported on the serological response after SARS-CoV-2 vaccination in patients with plasma-cell-related disorders, showing that antibody titers increased significantly after prime-boost vaccination in these patients compared to a healthy control group. A threshold of 30 CD19+ B lymphocytes per microliter was validated as predictive for antibody development. Despite certain negative factors, most myeloma patients were able to develop antibodies after vaccination.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemical Research Methods
Claudia Duranti, Elena Lastraioli, Jessica Iorio, Chiara Capitani, Laura Carraresi, Leonardo Gonnelli, Annarosa Arcangeli
Summary: Protein engineering has made significant advancements in biotechnology and pharmaceuticals, particularly with the development of engineered antibody subclasses. The single chain diabody format offers versatility in a variety of applications, but challenges include optimizing production, improving expression systems, purification procedures, and stability. Fine-tuning recombinant antibody expression through choosing the right protein expression host and adjusting expression protocols is crucial for success.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Biochemistry & Molecular Biology
Jorge A. Lerma Romero, Christian Meyners, Nicole Rupp, Felix Hausch, Harald Kolmar
Summary: This study used protein engineering to identify mutants with high affinity to conformation-specific FKBP51 selective ligands by randomizing selected regions of its ligand-binding domain. These improved mutants are valuable tools for discovering novel selective ligands and understanding the conformational dynamics and ligand binding mechanics of the FKBP51 binding pocket.
PROTEIN ENGINEERING DESIGN & SELECTION
(2023)
Review
Microbiology
Taruna Anand, Nitin Virmani, Bidhan C. Bera, Rajesh K. Vaid, Medhavi Vashisth, Priyanka Bardajatya, Ashok Kumar, Bhupendra N. Tripathi
Summary: Phage display is an important and effective molecular biology technique that has been widely used for screening vaccine candidates and developing diagnostic tools. This versatile technique has been modified over the years to target various pathogens, including coronaviruses such as SARS-CoV-2, motivating researchers to search for therapeutic candidates.
CURRENT MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michael B. Battles, Johannes P. Langedijk, Polina Furmanova-Hollenstein, Supranee Chaiwatpongsakorn, Heather M. Costello, Leen Kwanten, Luc Vranckx, Paul Vink, Steffen Jaensch, Tim H. M. Jonckers, Anil Koul, Eric Arnoult, Mark E. Peeples, Dirk Roymans, Jason S. McLellan
NATURE CHEMICAL BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Dirk Roymans, Sarhad S. Alnajjar, Michael B. Battles, Panchan Sitthicharoenchai, Polina Furmanova-Hollenstein, Peter Rigaux, Joke Van den Berg, Leen Kwanten, Marcia Van Ginderen, Nick Verheyen, Luc Vranckx, Steffen Jaensch, Eric Arnoult, Richard Voorzaat, Jack M. Gallup, Alejandro Larios-Mora, Marjolein Crabbe, Dymphy Huntjens, Pierre Raboisson, Johannes P. Langedijk, Mark R. Ackermann, Jason S. McLellan, Sandrine Vendeville, Anil Koul
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Daiyin Tian, Michael B. Battles, Syed M. Moin, Man Chen, Kayvon Modjarrad, Azad Kumar, Masaru Kanekiyo, Kevin W. Graepel, Noor M. Taher, Anne L. Hotard, Martin L. Moore, Min Zhao, Zi-Zheng Zheng, Ning-Shao Xia, Jason S. McLellan, Barney S. Graham
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Michael B. Battles, Vicente Mas, Eduardo Olmedillas, Olga Cano, Monica Vazquez, Laura Rodriguez, Jose A. Melero, Jason S. McLellan
NATURE COMMUNICATIONS
(2017)
Article
Medicine, Research & Experimental
Harald Rouha, Adriana Badarau, Zehra C. Visram, Michael B. Battles, Bianka Prinz, Zoltan Magyarics, Gabor Nagy, Irina Mirkina, Lukas Stulik, Manuel Zerbs, Michaela Jaegerhofer, Barbara Maierhofer, Astrid Teubenbacher, Ivana Dolezilkova, Karin Gross, Srijib Banerjee, Gerhild Zauner, Stefan Malafa, Jakub Zmajkovic, Sabine Maier, Robert Mabry, Eric Krauland, K. Dane Wittrup, Tillman U. Gerngross, Eszter Nagy
Article
Biotechnology & Applied Microbiology
Min Du, Michael B. Battles, Juergen H. Nett
Article
Microbiology
Harrison G. Jones, Michael B. Battles, Chun-Chi Lin, Siro Bianchi, Davide Corti, Jason S. McLellan
Article
Multidisciplinary Sciences
C. Garrett Rappazzo, Longping Tse, Chengzi Kaku, Daniel Wrapp, Mrunal Sakharkar, Deli Huang, Laura M. Deveau, Thomas J. Yockachonis, Andrew S. Herbert, Michael B. Battles, Cecilia M. O'Brien, Michael E. Brown, James C. Geoghegan, Jonathan Belk, Linghang Peng, Linlin Yang, Yixuan Hou, Trevor D. Scobey, Dennis R. Burton, David Nemazee, John M. Dye, James E. Voss, Bronwyn M. Gunn, Jason S. McLellan, Ralph S. Baric, Lisa E. Gralinski, Laura M. Walker
Summary: The engineered antibody ADG-2 shows enhanced neutralization breadth and potency against a wide range of sarbecoviruses, providing complete protection in SARS and COVID-19 mouse models. Structural and biochemical studies reveal that ADG-2 targets a highly conserved epitope through a unique angle of approach.