Article
Immunology
Lydia Verlaat, Katarina Riesner, Martina Kalupa, Beate Jung, Sarah Mertlitz, Constanze Schwarz, Joerg Mengwasser, Claudine Fricke, Olaf Penack
Summary: Despite progress in allo-HCT, cGvHD remains a major hurdle, and improved pre-clinical models are needed. We developed two murine cGvHD models that resemble the clinical manifestations of cGvHD in patients. These models exhibited diverse signs of cGvHD in target organs and decelerated immune cell reconstitution. They can provide a deeper understanding of cGvHD pathology and facilitate the translation of experimental findings into human allo-HCT.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lingyun Li, Kyrylo Krasnykov, David Homolka, Pascal Gos, Mateusz Mendel, Richard J. Fish, Radha Raman Pandey, Ramesh S. Pillai
Summary: The YTHDC2 protein plays a crucial role in mammalian fertility by binding to specific sites on the 3' UTRs of mouse testicular RNA targets. The loss of its 3'-> 5' RNA helicase activity leads to infertility in mice. Furthermore, its weak helicase activity is enhanced through interaction with the exoribonuclease XRN1. Studies on ythdc2 mutant zebrafish demonstrate its conserved role in animal germ cell development.
Article
Ecology
Qingsong Liu, Ted C. J. Turlings, Yunhe Li
Summary: Resource partitioning is crucial for the transition from competition to mutualism in coexisting species. However, this is not the case for two major pest insects of rice. These herbivores mutually benefit from each other by preferentially infesting the same host plants and utilizing them cooperatively through plant-mediated mechanisms.
TRENDS IN ECOLOGY & EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Uta Gehlsen, Daniela Stary, Martina Maass, Katarina Riesner, Gwen Musial, Michael E. Stern, Olaf Penack, Philipp Steven
Summary: oGVHD is a rapidly progressing autoimmune disease following hematopoietic stem cell transplantation that can lead to severe eye inflammation. Limited treatment options exist, making it crucial to develop new therapeutic approaches. A murine model with corneal epitheliopathy, lacrimal gland inflammation, and blepharitis was described, along with the observation of corneal lymphangiogenesis, suggesting potential therapeutic and diagnostic uses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Psychology, Biological
Kathelijne Koops, Aly Gaspard Soumah, Kelly L. van Leeuwen, Henry Didier Camara, Tetsuro Matsuzawa
Summary: This study conducted field experiments to investigate whether chimpanzees can individually (re-)innovate nut cracking. The results showed that chimpanzees did not independently (re-)innovate nut cracking under ecologically valid conditions, supporting the hypothesis that chimpanzee nut cracking is a product of social learning.
NATURE HUMAN BEHAVIOUR
(2022)
Letter
Medicine, General & Internal
Anna R. Schreiber, Dara L. Aisner, Jonathan A. Gutman
Summary: This article reports a case of fetal-induced graft-versus-host disease, in which the mother developed fever, pancytopenia, and rash 3 weeks after the delivery of twin sons that included transfusion.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Review
Immunology
Renata Auricchio, Riccardo Troncone
Summary: Celiac disease is an autoimmune disorder triggered by gluten, with prevention strategies focusing on identifying at-risk individuals and eliminating risk factors. Research has shown that both genetic and environmental factors play a role in the development of the disease, suggesting that future interventions could involve protection from infections, manipulation of microbiota, and modulation of T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Zhigang Liu, Qiang Zeng, Xinchuan Chen, Chuan He, Fangfang Wang, Ting Liu
Summary: Donor peritoneal-derived cells can attenuate graft-versus-host disease (GVHD) caused by bone marrow transplantation by regulating cytokine levels and immunocyte ratios.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biophysics
Jeremy L. Ramdial, Rohtesh S. Mehta, Rima M. Saliba, Amin M. Alousi, Qaiser Bashir, Chitra Hosing, Partow Kebriaei, Amanda L. Olson, Betul Oran, Muzaffar H. Qazilbash, Samer A. Srour, Borje S. Andersson, Richard E. Champlin, Uday Popat
Summary: The study identified that the majority of patients experiencing NRM between days 101 and 365 post-HCT had a history of grade II-IV acute GVHD, which was the leading cause of death. Acute GVHD was found to be the only significant predictor of NRM between days 101 and 365 in multivariate analysis. Measures to reduce the risk of acute GVHD could help lower the risk of NRM at 1 year and improve overall survival.
BONE MARROW TRANSPLANTATION
(2021)
Article
Biochemical Research Methods
Eran Stark, Amir Levi, Horacio G. Rotstein
Summary: Resonance is the maximal response of a neuronal system to periodic inputs in a limited frequency band. This study investigates how network resonance is generated and how it depends on the properties of network building blocks. The researchers find that network resonance can be inherited from resonances at other levels of organization or be intrinsically generated by combining mechanisms across distinct levels. At all levels of organization, interactions between low- and high-pass filters generate the observed resonance.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Medicine, General & Internal
J. Kellett, S. B. Bogh, U. Ekelund, M. Brabrand
Summary: The study found that ECG abnormalities increase linearly with age, especially between 30 and 70 years old. Patients aged 20-29 with abnormal ECG are more than four times likely to die within a year compared to those with normal ECG. After 70 years old, the relationship weakens, but for younger individuals, ECG abnormalities result in even higher mortality rates.
QJM-AN INTERNATIONAL JOURNAL OF MEDICINE
(2022)
Article
Plant Sciences
Fabiola Muro-Villanueva, Hoon Kim, John Ralph, Clint Chapple
Summary: Lignin is a major obstacle for biomass saccharification, but recent research has found that genetically altering lignin by knocking out two subunits of Mediator can alleviate the growth inhibition phenotype. This alteration leads to the synthesis of a lignin polymer derived from p-coumaryl alcohol.
Article
Immunology
Melrine Pereira, Natasha Ting Lee, Jonathan Noonan, Abbey E. H. Willcox, Ilaria Calvello, Smitha Rose Georgy, Carly Selan, Joanne S. Chia, Wayne Hauw, Xiaowei Wang, Karlheinz Peter, Simon C. Robson, Harshal H. Nandurkar, Maithili Sashindranath
Summary: Allogenic hematopoietic stem cell transplant can result in sinusoidal obstruction syndrome and graft-versus-host disease. Endothelial activation is an early sign of acute GvHD and SOS in a clinically relevant mouse model based on myeloablative chemotherapy. Preventing endothelial activation may be a viable therapeutic strategy to prevent GvHD.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Shota Shimizu, Shinri Sato, Hiroko Taniguchi, Eisuke Shimizu, Jingliang He, Shunsuke Hayashi, Kazuno Negishi, Yoko Ogawa, Shigeto Shimmura
Summary: The study observed the dynamics of inflammatory cells in the cornea of chronic GVHD model mice, discovering that neovascularization occurred in all eyes at 1 week after BMT but almost all vessels disappeared at 2 weeks after BMT. Additionally, infiltration of globular cells and tortuosity and branching of nerves were found in both cGVHD mice and human cGVHD patients.
Review
Immunology
Qingxiao Song, Ubaydah Nasri, Defu Zeng
Summary: This review provides an overview of the current understanding of the pathogenesis of steroid-refractory gut graft-versus-host disease, advanced technologies for human studies, and potential novel therapeutic options.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Renan V. H. de Carvalho, Djalma S. Lima-Junior, Caroline de Oliveira, Dario S. Zamboni
Summary: The New World species of intracellular protozoan parasites in the Leishmania genus can cause mucocutaneous leishmaniases. The presence of Leishmania RNA virus (LRV) in Leishmania guyanensis (L.g.) exacerbates disease and leads to the development of mucocutaneous disease. Research has shown that LRV inhibits caspase-11 activation by Leishmania, shedding light on the mechanisms through which LRV promotes disease exacerbation.
Article
Biochemistry & Molecular Biology
Ellen Kiarely Souza, Filipe S. Pereira-Dutra, Matheus A. Rajao, Felipe Ferraro-Moreira, Taynna C. Goltara-Gomes, Tamires Cunha-Fernandes, Julia da Cunha Santos, Elisa B. Prestes, Warrison A. Andrade, Dario S. Zamboni, Marcelo T. Bozza, Patricia T. Bozza
Summary: The study found that LD formation in macrophages infected with Salmonella Typhimurium rapidly increased, depending on the viability of Salmonella and T3SS activity, and regulated by TLR signaling. LD accumulation occurs through TLR2-dependent signaling and is counter-regulated by TLR4.
MOLECULAR MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Tiago J. Costa, Simone R. Potje, Thais F. C. Fraga-Silva, Julio A. Da Silva-Neto, Paula R. Barros, Daniel Rodrigues, Mirele R. Machado, Ronaldo B. Martins, Rosangela A. Santos-Eichler, Maira N. Benatti, Keyla S. G. de Sa, Carlos Eduardo L. Almado, Italo A. Castro, Marjorie C. Pontelli, Leonardo La Serra, Fernando S. Carneiro, Christiane Becari, Paulo Louzada-Junior, Rene D. R. Oliveira, Dario S. Zamboni, Eurico Arruda, Maria Auxiliadora-Martins, Fernanda R. C. Giachini, Vania L. D. Bonato, Natasha E. Zachara, Gisele F. Bomfim, Rita C. Tostes
Summary: This study demonstrates that SARS-CoV-2 infection impairs mitochondrial function and activates TLR9 signaling in endothelial cells, leading to inflammatory responses and endothelial dysfunction. Targeting mitochondrial metabolic pathways may offer new therapeutic strategies for COVID-19.
VASCULAR PHARMACOLOGY
(2022)
Article
Oncology
Sabrina S. Batah, Maira N. Benatti, Li Siyuan, Wagner M. Telini, Jamile O. Barboza, Marcelo B. Menezes, Tales R. Nadai, Keyla S. G. Sa, Chirag M. Vaswani, Sahil Gupta, Dario S. Zamboni, Danilo T. Wada, Rodrigo T. Calado, Rene D. R. Oliveira, Paulo Louzada-Junior, Maria Auxiliadora-Martins, Flavio P. Veras, Larissa D. Cunha, Thiago M. Cunha, Rodrigo Luppino-Assad, Marcelo L. Balancin, Sirlei S. Morais, Ronaldo B. Martins, Eurico Arruda, Fernando Chahud, Marcel Koenigkam Santos, Andrea A. Cetlin, Fernando Q. Cunha, Claudia dos Santos, Vera L. Capelozzi, Junya Fukuoka, Rosane Duarte Achcar, Alexandre T. Fabro
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Marjorie C. Pontelli, Italo A. Castro, Ronaldo B. Martins, Leonardo La Serra, Flavio P. Veras, Daniele C. Nascimento, Camila M. Silva, Ricardo S. Cardoso, Roberta Rosales, Rogerio Gomes, Thais M. Lima, Juliano P. Souza, Brenda C. Vitti, Diego B. Caetite, Mikhael H. F. de Lima, Spencer D. Stumpf, Cassandra E. Thompson, Louis-Marie Bloyet, Juliana E. Toller-Kawahisa, Marcela C. Giannini, Leticia P. Bonjorno, Maria I. F. Lopes, Sabrina S. Batah, Li Siyuan, Rodrigo Luppino-Assad, Sergio C. L. Almeida, Fabiola R. Oliveira, Maira N. Benatti, Lorena L. F. Pontes, Rodrigo C. Santana, Fernando C. Vilar, Maria Auxiliadora-Martins, Pei-Yong Shi, Thiago M. Cunha, Rodrigo T. Calado, Jose C. Alves-Filho, Dario S. Zamboni, Alexandre T. Fabro, Paulo Louzada-Junior, Rene D. R. Oliveira, Sean P. J. Whelan, Fernando Q. Cunha, Eurico Arruda
Summary: SARS-CoV-2 infection can lead to lymphocytopenia and infect immune cells such as monocytes, B cells, and T cells, resulting in T cell apoptosis. Additionally, SARS-CoV-2 is frequently detected in monocytes and B cells from COVID-19 patients.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Biology
Ana C. G. Salina, Douglas dos-Santos, Tamara S. Rodrigues, Marlon Fortes-Rocha, Edismauro G. Freitas-Filho, Daniel L. Alzamora-Terrel, Icaro M. S. Castro, Thais F. C. Fraga da Silva, Mikhael H. F. de Lima, Daniele C. Nascimento, Camila M. Silva, Juliana E. Toller-Kawahisa, Amanda Becerra, Samuel Oliveira, Diego B. Caetite, Leticia Almeida, Adriene Y. Ishimoto, Thais M. Lima, Ronaldo B. Martins, Flavio Veras, Natalia B. do Amaral, Marcela C. Giannini, Leticia P. Bonjorno, Maria I. F. Lopes, Maira N. Benatti, Sabrina S. Batah, Rodrigo C. Santana, Fernando C. Vilar, Maria A. Martins, Rodrigo L. Assad, Sergio C. L. de Almeida, Fabiola R. de Oliveira, Eurico Arruda Neto, Thiago M. Cunha, Jose C. Alves-Filho, Vania L. D. Bonato, Fernando Q. Cunha, Alexandre T. Fabro, Helder Nakaya, Dario S. Zamboni, Paulo Louzada-Junior, Rene D. R. Oliveira, Larissa D. Cunha
Summary: This study reveals that the engulfment of SARS-CoV-2-infected apoptotic cells by macrophages exacerbates inflammatory cytokine production and impairs the ability of macrophages to perform continual efferocytosis. Lung monocytes and macrophages from severe COVID-19 patients also show compromised efferocytic capacity.
Article
Multidisciplinary Sciences
Leticia de Almeida, Alexandre L. N. da Silva, Tamara S. Rodrigues, Samuel Oliveira, Adriene Y. Ishimoto, Amanda A. Seribelli, Amanda Becerra, Warrison A. Andrade, Marco A. Ataide, Camila C. S. Caetano, Keyla S. G. de Sa, Natalia Pelisson, Ronaldo B. Martins, Juliano de Paula Souza, Eurico Arruda, Sabrina S. Batah, Ricardo Castro, Fabiani G. Frantz, Fernando Q. Cunha, Thiago M. Cunha, Alexandre T. Fabro, Larissa D. Cunha, Paulo Louzada-Junior, Rene D. R. de Oliveira, Dario S. Zamboni
Summary: This study identifies niclosamide as a potential FDA-approved drug for treating COVID-19 by inhibiting inflammasome activation and SARS-CoV-2 replication. Niclosamide shows strong inhibitory effects on inflammasome activation in human monocytes and PBMCs from COVID-19 patients in vitro, as well as in an in vivo mouse model of SARS-CoV-2 infection.
Article
Immunology
Marco A. Ataide, Graziele Z. Manin, Samuel S. Oliveira, Rhanoica O. Guerra, Dario S. Zamboni
Summary: Flagellin-induced activation of NAIP/NLRC4 inflammasome and pyroptosis play critical roles in restricting Legionella pneumophila infection. In this study, we found temporal coordination of two independent innate immunity pathways, inflammasome activation and CCR2-mediated recruitment of Mo-DC, in controlling the infection. The inflammasome activation is important at the early stage of infection, while Mo-DC recruitment is crucial for efficient bacterial clearance at the late stage. Mo-DC emergence depends on CCR2-signaling and is independent of inflammasome activation and pyroptosis. Additionally, Mo-DC compartment produces the most cytokines among monocyte-derived cells in the infected lung. The simultaneous ablation of CCR2 and NLRC4 axes results in aggravated bacterial burden in the lung, indicating the interplay between inflammasome activation and CCR2-mediated immune response in restricting bacterial infection.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Oncology
Sandra P. Palma Albornoz, Thais F. C. Fraga-Silva, Renan V. H. de Carvalho, Tamara S. Rodrigues, Ana Flavia Gembre, Romulo Silva de Oliveira, Fernanda Mesquita de Souza, Giseli Furlan Correa, Leandra N. Z. Ramalho, Daniela Carlos, Danilo C. de Almeida, Niels O. S. Camara, Dario S. Zamboni, Viviani Nardini Takahashi, Carlos A. Sorgi, Lucia H. Faccioli, Alexandra Medeiros, Diego Luis Costa, Vania L. D. Bonato
Summary: A low-grade and persistent inflammation in obesity requires the participation of NLRP3 and cell death. However, during obesity-tuberculosis comorbidity, NLRP3 signaling may play a detrimental role by inducing excessive inflammation. The study shows that NLRP3 signaling promotes cell death without affecting bacterial loads.
JOURNAL OF PATHOLOGY
(2023)
Article
Microbiology
Raiany A. Santos, Daiane M. Cerqueira, Dario S. Zamboni, Sergio C. Oliveira
Summary: This study reveals the key role of caspase-7 in cell death during Brucella infection and highlights the important function of caspase-8 in inflammasome activation and pyroptosis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
N. B. Amaral, T. S. Rodrigues, M. C. Giannini, M. I. Lopes, L. P. Bonjorno, P. I. S. O. Menezes, S. M. Dib, S. L. G. Gigante, M. N. Benatti, U. C. Rezek, L. L. Emrich-Filho, B. A. Sousa, S. C. L. Almeida, R. Luppino-Assad, F. P. Veras, A. H. Schneider, L. O. S. Leiria, L. D. Cunha, J. C. Alves-Filho, T. M. Cunha, E. Arruda, C. H. Miranda, A. Pazin-Filho, M. Auxiliadora-Martins, M. C. Borges, B. A. L. Fonseca, V. R. Bollela, C. M. Del-Ben, F. Q. Cunha, R. C. Santana, F. C. Vilar, D. S. Zamboni, P. Louzada-Junior, R. D. R. Oliveira
Summary: This study evaluated whether colchicine treatment was associated with the inhibition of NLRP3 inflammasome activation in patients with COVID-19. The results showed that colchicine reduced the need for supplemental oxygen and the length of hospitalization, and lowered the serum levels of Casp1p20 and IL-18 in the first two days of treatment.
INFLAMMATION RESEARCH
(2023)
Article
Immunology
Tamara S. Rodrigues, Camila C. S. Caetano, Keyla S. G. de Sa, Leticia Almeida, Amanda Becerra, Augusto Goncalves, Leticia de Sousa Lopes, Samuel Oliveira, Danielle P. A. Mascarenhas, Sabrina S. Batah, Bruna M. Silva, Giovanni F. Gomes, Ricardo Castro, Ronaldo B. Martins, Jonathan Avila, Fabiani G. Frantz, Thiago M. Cunha, Eurico Arruda, Fernando Q. Cunha, Helder Nakaya, Larissa D. Cunha, Alexandre T. Fabro, Paulo Louzada-Junior, Rene D. R. de Oliveira, Dario S. Zamboni
Summary: SARS-CoV-2 infection activates the NLRP3 inflammasome and leads to inflammation and severe COVID-19. Human caspase-4 (mouse caspase-11) is upregulated and activated by SARS-CoV-2, contributing to NLRP3 activation. In vivo studies using transgenic hACE2 humanized mice show that hACE2 Casp11(-/-) mice are protected from disease development, with reduced lung damage, clinical symptoms, and mortality. Analysis of human samples from fatal COVID-19 cases reveals the expression of CASP4 in patient lungs, which correlates with inflammasome components and inflammatory mediators. These findings suggest CASP4/11 as a potential target for therapeutic interventions in COVID-19.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Keyla S. G. de Sa, Luana A. Amaral, Tamara S. Rodrigues, Adriene Y. Ishimoto, Warrison A. C. de Andrade, Leticia de Almeida, Felipe Freitas-Castro, Sabrina S. Batah, Sergio C. Oliveira, Monica T. Pastorello, Alexandre T. Fabro, Dario S. Zamboni
Summary: In this study, it is demonstrated that Gasdermin-D (GSDMD) is activated during the early stages of Leishmania infection, promoting NLRP3 inflammasome activation without causing cell death. GSDMD is cleaved into a noncanonical fragment, indicating the subversion of host response by Leishmania. Skin biopsies from patients with leishmaniasis also show activation of NLRP3 inflammasome and GSDMD.
NATURE COMMUNICATIONS
(2023)
Article
Critical Care Medicine
Camila Meirelles S. Silva, Carlos Wagner S. Wanderley, Flavio Protasio Veras, Augusto Veloso Goncalves, Mikhael Haruo Fernandes Lima, Juliana Escher Toller Kawahisa, Giovanni Freitas Gomes, Daniele Carvalho Nascimento, Valter V. Silva Monteiro, Isadora Marques Paiva, Cicero Jose Luiz Ramos Almeida, Diego Brito Caetite, Juliana Costa Silva, Maria Isabel Fernandes Lopes, Leticia Pastorelli Bonjorno, Marcela Cavichioli Giannini, Natalia Brasil Amaral, Maira Nilson Benatti, Rodrigo Carvalho Santana, Luis Eduardo Alves Damasceno, Bruna Manuella Souza Silva, Ayda Henriques Schneider, Icaro Maia Santos Castro, Juan Carlo Santos Silva, Amanda Pereira Vasconcelos, Tiago Tomazini Goncalves, Sabrina Setembre Batah, Tamara Silva Rodrigues, Victor Ferreira Costa, Marjorie Cornejo Pontelli, Ronaldo B. Martins, Timna Varela Martins, Danillo Lucas Alves Esposito, Guilherme Cesar Martelossi Cebinelli, Benedito Antonio Lopes da Fonseca, Luiz Osorio Silveira Leiria, Larissa Dias Cunha, Eurico Arruda, Helder Nakaia, Alexandre Todorovic Fabro, Rene D. R. Oliveira, Dario S. Zamboni, Paulo Louzada-Junior, Thiago Mattar Cunha, Jose Carlos Farias Alves-Filho, Fernando Queiroz Cunha
Summary: This study reveals that SARS-CoV-2 virus directly activates GSDMD, leading to the production of NETs and organ damage in COVID-19. Single-cell transcriptome analysis shows increased expression of GSDMD and inflammasome-related genes in COVID-19 patients. Treatment with disulfiram inhibits NETs release and reduces organ damage in a mouse model of SARS-CoV-2 infection.
Article
Immunology
Mikhael Haruo Fernandes de Lima, Carlos Hiroji Hiroki, Vanessa de Fatima Borges, Guilherme Cesar Martelossi Cebinelli, Jessica Santos, Marcos Henrique Rosa, Camila Meirelles S. Silva, Carlos Wagner S. Wanderley, Augusto Velozo Goncalves, Gustavo Fernando Silva Quirino, Dario S. Zamboni, Thiago M. Cunha, Jose-Carlos A. Filho, Fernando Q. Cunha
Summary: Our study demonstrates that interleukin 33 is pivotal in selectively expanding the T-cell immunoglobulin and ITIM domain (TIGIT)(+) T-regulatory cell repertoire in a STAT6 and M2 macrophage-dependent fashion.
JOURNAL OF INFECTIOUS DISEASES
(2022)