Article
Biochemistry & Molecular Biology
Jannik Jahn, Antonia Bollensdorf, Christopher Kalischer, Robin Piecha, Jana Weiss-Mueller, Phani Sankar Potru, Tamara Russ, Bjoern Spittau
Summary: It has been found that TGF beta regulates the activation of microglia by inhibiting the expression of Cd74 in these cells. Additionally, silencing of TGF beta signaling leads to upregulation of CD74, further demonstrating the importance of microglial TGF beta signaling in regulating microglia activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xin Zhou, Junhong Li, Ludvig J. Backman, Patrik Danielson
Summary: This study investigated the effects of IL-1 beta and TGF beta 1 on corneal stromal cell differentiation. IL-1 beta treatment reduced the keratocyte phenotype, while TGF beta 1 treatment induced differentiation towards myofibroblasts. The crosstalk between IL-1 beta and TGF beta 1 was mediated by the NF-kappa B signaling pathway, with IL-1 beta inhibiting TGF beta 1-induced keratocyte-myofibroblast differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wen-qing Tian, Si-yu Chen, Feng-ning Chuan, Wen-rui Zhao, Bo Zhou
Summary: TINAGL1 plays an important role in diabetic wound healing and its expression is regulated by high glucose. Administration of exogenous TINAGL1 can promote wound healing in diabetic mice. These findings provide new insights for the treatment of diabetic wounds.
Article
Multidisciplinary Sciences
Aristides Lopez-Marquez, Carlos Carrasco-Lopez, Andrea Martinez-Cano, Pascale Lemoine, Christophe E. Pierreux, Pilar Santisteban
Summary: This study investigates the role of the transcription factor Sox9 in the differentiation of thyroid follicular cells. The results show that TSH and TGF beta signals regulate the expression of Sox9, and the thyroid transcription factor Pax8 also plays a role in the transcription of Sox9.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Xiaoshuang Yang, Jianting Guo, Wei Li, Chunrui Li, Xilin Zhu, Ying Liu, Xiaopan Wu
Summary: We found that ATF6 can inhibit the expression of PPM1H mRNA and protein in hepatoma cells and mice with liver Atf6 knockdown. Immunohistochemical analysis of tumor tissues from 134 HCC patients showed higher expression of PPM1H in adjacent para-cancer tissues than in HCC tissues. PPM1H exhibited a suppressive effect on HCC progression by inhibiting proliferation, migration, and invasion of hepatoma cells.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biology
Elena Sanchez-Escabias, Jose A. Guerrero-Martinez, Jose C. Reyes
Summary: This study investigates the co-transcriptional splicing (CTS) efficiency of different introns within the same gene using nascent chromatin-associated RNA-sequencing data. The results show that the CTS efficiency tends to be similar between different introns of a gene, indicating a coordinated mechanism. Furthermore, the study demonstrates that the TGF beta signal transduction pathway can regulate the general CTS efficiency and impact mature mRNA levels. Overall, CTS efficiency is a gene-specific characteristic that can be controlled to regulate gene expression.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Junwon Kim, Mark D. Rose
Summary: In Saccharomyces cerevisiae, the p21-activated kinase Cla4p regulates polarized morphogenesis and cytokinesis. Cla4p kinase activity is down-regulated during mating, and this is achieved through phosphorylation of Cla4p at multiple sites, including Cla4p-S186, which reduces its kinase activity. Fus3p counteracts Cla4p kinase activity during mating by phosphorylating Fus2p at different sites to promote nuclear export and block import.
Article
Obstetrics & Gynecology
Rafiatu Azumah, Menghe Liu, Katja Hummitzsch, Nicole A. Bastian, Monica D. Hartanti, Helen F. Irving-Rodgers, Richard A. Anderson, Raymond J. Rodgers
Summary: This study investigates the changes in TGF beta signalling during foetal ovary development and its potential influence on the expression of PCOS candidate genes. The findings suggest that TGF beta 1 inhibits the expression of certain PCOS candidate genes in foetal ovarian fibroblasts, while increasing the expression of an AR cofactor. The study highlights the importance of TGF beta signalling in the development of PCOS.
HUMAN REPRODUCTION
(2022)
Article
Immunology
Xinying Guo, Negar Khosraviani, Sneha Raju, Joshya Singh, Nikki Zamani Farahani, Madlene Abramian, Victor J. Torres, Kathryn L. Howe, Jason E. Fish, Andras Kapus, Warren L. Lee
Summary: English Summary: ACKR1 is a cell surface protein expressed on erythrocytes and endothelial cells, and is involved in regulating innate immunity. Exposure to whole blood induces ACKR1 mRNA and protein expression in primary human lung microvascular endothelial cells, which requires contact with neutrophils. ACKR1 expression is regulated by NF-kappa B, and the protein is rapidly secreted by extracellular vesicles upon removal of blood. Endogenous ACKR1 does not signal upon stimulation with IL-8 or CXCL1. These findings provide a simple method for inducing endogenous endothelial ACKR1 protein and facilitate further functional studies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Zi-Yang Zhang, Dilshan S. Harischandra, Ruifang Wang, Shivani Ghaisas, Janet Y. Zhao, Thomas P. McMonagle, Guixin Zhu, Kenzo D. Lacuarta, Jianing Song, John Q. Trojanowski, Hong Xu, Virginia M. Y. Lee, Xiaolu Yang
Summary: The down-regulation of TRIM11 is implicated in the pathogenesis of tauopathies, and restoring TRIM11 expression can effectively ameliorate pathological changes and cognitive impairments in animal models of tauopathies.
Article
Multidisciplinary Sciences
Stefanie Fruhwurth, Line S. Reinert, Carl Oberg, Marcelina Sakr, Marcus Henricsson, Henrik Zetterberg, Soren R. Paludan
Summary: Immunological control of viral infections in the brain, especially through the TREM2 pathway, is crucial for protection against herpes simplex virus type 1 (HSV1) infection and maintenance of brain integrity. The down-regulation of TREM2 expression in HSV1-infected microglia leads to impaired antiviral response and decreased ability to phagocytose infected neurons. This study highlights the importance of TREM2 in the immune defense against viral infections and raises questions about its role in neurological pathologies.
Article
Cell Biology
Xiufeng Chu, Xiang Xiao, Guangchuan Wang, Ahmed Uosef, Xiaohua Lou, Preston Arnold, Yixuan Wang, Gangcheng Kong, Mou Wen, Laurie J. Minze, Xian C. C. Li
Summary: This study shows that the cytolytic activity of Gasdermin D N-terminal pore-forming domain (GD-NT) is negatively regulated by AMP-activated protein kinase (AMPK). AMPK phosphorylates GD-NT at serine 46, which alters its oligomerization and inhibits its pore-forming ability. The negative regulation of GD-NT by AMPK impacts its anti-tumor activity and promotes aggressive tumor growth.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Viera Horvathova Kajabova, Andrea Soltysova, Lucia Demkova, Paulina Plesnikova, Darina Lyskova, Alena Furdova, Pavel Babal, Bozena Smolkova
Summary: Our study found that KIT positivity was associated with a more than 17-fold increase in the risk of poor prognosis in uveal melanoma, and a strong negative correlation was identified between mRNA expression and DNA methylation values for 12 of 20 analyzed positions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Elizabeth D. Howell, Amanda D. Yzaguirre, Peng Gao, Raphael Lis, Bing He, Melike Lakadamyali, Shahin Rafii, Kai Tan, Nancy A. Speck
Summary: The study highlights the role of RUNX1 in hematopoiesis, showing its ability to recruit TGFβ signaling effectors in the endothelial-to-hematopoietic transition. The efficiency of this transition is influenced by chromatin changes in endothelial cells at different developmental stages.
GENES & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Ming Li, Ziming Liu, Jia Song, Tian Wang, Hongjie Wang, Yanan Wang, Jiguang Guo
Summary: This study identified key pathways and genes involved in the initiation and progression of colorectal cancer (CRC) using bioinformatics methods. The extracellular tumor microenvironment and bile metabolism pathways were found to play critical roles in CRC. ADH1C was identified as a prognostic predictor for CRC.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shana Claeys, Geertrui Denecker, Kaat Durinck, Bieke Decaesteker, Liselot M. Mus, Siebe Loontiens, Suzanne Vanhauwaert, Kristina Althoff, Caroline Wigerup, Daniel Bexell, Emmy Dolman, Kai-Oliver Henrich, Lea Wehrmann, Ellen M. Westerhout, Jean-Baptiste Demoulin, Candy Kumps, Tom Van Maerken, Genevieve Laureys, Christophe Van Neste, Bram De Wilde, Olivier De Wever, Frank Westermann, Rogier Versteeg, Jan J. Molenaar, Sven Pahlman, Johannes H. Schulte, Katleen De Preter, Frank Speleman
Article
Dermatology
Guillaume Dachy, Ronald R. de Krijger, Sylvie Fraitag, Ivan Theate, Benedicte Brichard, Suma B. Hoffman, Louis Libbrecht, Florence A. Arts, Pascal Brouillard, Miikka Vikkula, Nisha Limaye, Jean-Baptiste Demoulin
Article
Biochemistry & Molecular Biology
Muntasir Billah, Anisyah Ridiandries, Usaid K. Allahwala, Harshini Mudaliar, Anthony Dona, Stephen Hunyor, Levon M. Khachigian, Ravinay Bhindi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Immunology
Martijn J. Schuijs, Shaun Png, Arianne C. Richard, Anastasia Tsyben, Gregory Hamm, Julie Stockis, Celine Garcia, Silvain Pinaud, Ashley Nicholls, Xavier Romero Ros, Jing Su, Matthew D. Eldridge, Angela Riedel, Eva M. Serrao, Hans-Reimer Rodewald, Matthias Mack, Jacqueline D. Shields, E. Suzanne Cohen, Andrew N. J. McKenzie, Richard J. A. Goodwin, Kevin M. Brindle, John C. Marioni, Timotheus Y. F. Halim
Article
Oncology
Simone Hettmer, Guillaume Dachy, Guido Seitz, Abbas Agaimy, Catriona Duncan, Marjolijn Jongmans, Steffen Hirsch, Iris Kventsel, Uwe Kordes, Ronald R. de Krijger, Markus Metzler, Orli Michaeli, Karolina Nemes, Anna Poluha, Tim Ripperger, Alexandra Russo, Stephanie Smetsers, Monika Sparber-Sauer, Eveline Stutz, Franck Bourdeaut, Christian P. Kratz, Jean-Baptiste Demoulin
Summary: Infantile myofibromatosis (IM) presents a wide clinical spectrum, ranging from inconspicuous solitary soft tissue nodules to life-threatening complications. Familial IM follows an autosomal dominant mode of inheritance and is linked to PDGFRB germline variants. Mutant receptors remain sensitive to tyrosine kinase inhibitors and require a second hit for full activation.
Letter
Dermatology
A. Onoufriadis, B. Boulouadnine, G. Dachy, T. Higashino, H. Y. Huang, C. K. Hsu, M. A. Simpson, K. Bork, J. B. Demoulin, J. A. McGrath
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Emilie Guerit, Florence Arts, Guillaume Dachy, Boutaina Boulouadnine, Jean-Baptiste Demoulin
Summary: PDGFRA and PDGFRB are classical proto-oncogenes encoding receptor tyrosine kinases, with mutations linked to various tumors and diseases. Research has validated the effectiveness of tyrosine kinase inhibitors targeting PDGF receptors, such as imatinib, as a treatment for certain conditions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Geriatrics & Gerontology
Morgane M. Thibaut, Martina Sboarina, Martin Roumain, Sarah A. Potgens, Audrey M. Neyrinck, Florence Destree, Justine Gillard, Isabelle A. Leclercq, Guillaume Dachy, Jean-Baptiste Demoulin, Anne Tailleux, Sophie Lestavel, Marialetizia Rastelli, Amandine Everard, Patrice D. Cani, Paolo E. Porporato, Audrey Loumaye, Jean-Paul Thissen, Giulio G. Muccioli, Nathalie M. Delzenne, Laure B. Bindels
Summary: This study identified alterations in bile acid metabolism and hepatobiliary secretion in cancer cachexia, with an increase in systemic inflammation contributing to impairments in the hepatobiliary transport system. Targeting bile acids using cholestyramine reduced hepatic inflammation, while reducing interleukin 6 levels counteracted changes in gene expression related to hepatobiliary transport, bile acid synthesis, and inflammation. Serum bile acid levels were increased in cachectic cancer patients and strongly correlated with systemic inflammation.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Multidisciplinary Sciences
Karsten Bach, Sara Pensa, Marija Zarocsinceva, Katarzyna Kania, Julie Stockis, Silvain Pinaud, Kyren A. Lazarus, Mona Shehata, Bruno M. Simoes, Alice R. Greenhalgh, Sacha J. Howell, Robert B. Clarke, Carlos Caldas, Timotheus Y. F. Halim, John C. Marioni, Walid T. Khaled
Summary: The study demonstrates that mutations in BRCA1 can lead to aberrant differentiation of luminal progenitors towards a partial secretory luminal cell phenotype, occurring in early stages of tumour initiation and in breast cells from BRCA1 carriers.
NATURE COMMUNICATIONS
(2021)
Review
Cardiac & Cardiovascular Systems
Alexandre Persu, Piotr Dobrowolski, Heather L. Gornik, Jeffrey W. Olin, David Adlam, Michel Azizi, Pierre Boutouyrie, Rosa Maria Bruno, Marion Boulanger, Jean-Baptiste Demoulin, Santhi K. Ganesh, Tomasz J. Guzik, Magdalena Januszewicz, Jason C. Kovacic, Mariusz Kruk, Peter de Leeuw, Bart L. Loeys, Marco Pappaccogli, Melanie H. A. M. Perik, Emmanuel Touze, Patricia Van der Niepen, Daan J. L. Van Twist, Ewa Warchol-Celinska, Aleksander Prejbisz, Andrzej Januszewicz
Summary: Fibromuscular dysplasia (FMD) is a non-atherosclerotic vascular disease that predominantly affects women. Its exact cause is still poorly understood, but it is likely to involve a combination of genetic and environmental factors, leading to arterial fibrosis, cellular hyperplasia, and distortion of the arterial wall architecture. FMD can affect various arteries throughout the body, not just the renal and cerebrovascular arteries.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Emeline Bollaert, Melissa Claus, Virginie Vandewalle, Sandrine Lenglez, Ahmed Essaghir, Jean-Baptiste Demoulin, Violaine Havelange
Summary: The study found that miR-15a-5p induces chemoresistance in AML cells by inhibiting daunorubicin-induced autophagy, suggesting that this miRNA could be a promising therapeutic target for chemoresistant AML patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Guillaume Dachy, Sylvie Fraitag, Boutaina Boulouadnine, Sabine Cordi, Jean-Baptiste Demoulin
Summary: The study identified different gene fusions in myofibroma patients, including a novel COL4A1-VEGFD fusion, which provides new insights for the diagnosis and treatment of myofibroma.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Oncology
Melanie Gaignage, Xuhao Zhang, Julie Stockis, Olivier Dedobbeleer, Camille Michiels, Perrine Cochez, Laure Dumoutier, Pierre G. Coulie, Sophie Lucas
Summary: The transmembrane protein GARP binds latent TGF-beta 1 to form complexes on the surface of Tregs, B-cells, and platelets, which release active TGF-beta 1 upon stimulation. Blocking TGF-beta 1 activation by Tregs with anti-GARP:TGF-beta 1 mAbs overcomes resistance to PD1/PD-L1 blockade in murine tumors. Furthermore, antibody-mediated blockade of GARP:TGF-beta 1 or genetic deletion of Garp in Tregs, B cells, or platelets does not alter immune responses in mice, suggesting potential for cancer immunotherapy without impairing other immune defenses.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Cell Biology
Audrey Nedelec, Emilie M. Guerit, Guillaume Dachy, Sandrine Lenglez, Lok San Wong, Florence A. Arts, Jean-Baptiste Demoulin
Summary: Penttinen syndrome is a rare progeroid disorder caused by mutations in the PDGF receptor beta gene. This study demonstrates that the PDGFRB p.Val665Ala variant has unique features at the molecular level, which may be associated with the symptoms of Penttinen syndrome.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Connor H. O'Meara, Zuhayr Jafri, Levon M. Khachigian
Summary: Immune checkpoint inhibitor (ICI) therapy has greatly improved the treatment of various cancers, including head and neck cancers. However, there are still challenges in achieving a durable response and overcoming toxic effects. A deeper understanding of the interactions between immune cells, tumor cells, and the tumor microenvironment is necessary to develop more effective therapies. This article explores the strengths and limitations of current ICI therapy in head and neck squamous cell carcinoma (HNSCC), as well as emerging small-molecule immunotherapies and the potential of neutrophil extracellular traps in controlling tumor progression and metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
