Review
Neurosciences
Pragnya Chakraborty, Gaiti Hasan
Summary: Key components of endoplasmic reticulum (ER) Ca2+ release and store-operated Ca2+ entry (SOCE) are expressed in all metazoan cells. This review presents evidence on how these two mechanisms impact neuronal physiology and their interaction with ion channels in neurons. The careful regulation of neural SOCE is essential due to the detrimental effects of Ca2+ imbalance on neurons. The study also discusses different mechanisms of SOCE regulation in Drosophila and mammalian neurons, and their significance in human neurodegenerative diseases.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Multidisciplinary Sciences
Kyle E. Harvey, Shiqi Tang, Emily K. LaVigne, Evan P. S. Pratt, Gregory H. Hockerman
Summary: In this study, the researchers found that RyR2 is required for the regulation of insulin content and glucose-stimulated insulin secretion in INS-1 cells, partly through its interaction with IRBIT. The experiments showed that the absence of RyR2 led to a decrease in store-operated Ca2+ entry and PLC activity, as well as an increase in cellular PIP2 levels. Furthermore, RyR2 was found to regulate beta-cell electrical activity by controlling Ca-v current density and SK channel activation.
Article
Computer Science, Artificial Intelligence
Randall Clark, Lawson Fuller, Jason A. Platt, Henry D. I. Abarbanel
Summary: This article introduces how to use methods from nonlinear dynamics and interpolation techniques from applied mathematics to construct discrete time dynamical rules that forecast observed neuron properties accurately, which is crucial for the development of neural network models and biological networks.
NEURAL COMPUTATION
(2022)
Article
Biochemistry & Molecular Biology
Laura Marongiu, Francesca Mingozzi, Clara Cigni, Roberta Marzi, Marco Di Gioia, Massimiliano Garre, Dario Parazzoli, Laura Sironi, Maddalena Collini, Reiko Sakaguchi, Takashi Morii, Mariacristina Crosti, Monica Moro, Stephane Schurmans, Tiziano Catelani, Rany Rotem, Miriam Colombo, Stephen Shears, Davide Prosperi, Ivan Zanoni, Francesca Granucci
Summary: Recognition of LPS by DCs involves the receptor complex CD14 and TLR4, and subsequent Ca2+ mobilization and NFAT activation require IP3R3 and ITPKB. Targeting ITPKB may offer a promising approach for anti-inflammatory therapies aimed at inhibiting specific DC functions.
Article
Multidisciplinary Sciences
Nagendra Babu Thillaiappan, Holly A. Smith, Peace Atakpa-Adaji, Colin W. Taylor
Summary: The study demonstrates that the KRas-induced KRAP protein tethers a subset of IP3 receptors to actin, enabling them to evoke calcium signals. Loss of KRAP results in the disappearance of Ca2+ puffs and a global response to increased cytosolic Ca2+ concentration from more intense stimulation.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Rishav Mitra, Gaiti Hasan
Summary: Recent studies have shown that store-operated Ca2+ entry plays an important role in regulating neuronal function in Drosophila and mouse, with changes in ion channel function observed in neurons when this entry is lost. These changes are specific to neuronal subtypes. Gene expression studies in the mouse brain have also revealed differences in the expression of genes involved in ER-store Ca2+ release and store-operated Ca2+ entry across different neuronal classes. Loss of store-operated Ca2+ entry in neurons affects neuronal gene expression profiles, including genes encoding ion channels. Further research is needed to understand the functional significance of store-operated Ca2+ entry in specific neuronal subtypes and in the context of neurodegenerative syndromes.
CURRENT OPINION IN NEUROBIOLOGY
(2022)
Article
Cell Biology
Ana M. Rossi, Andrew M. Riley, Genevieve Dupont, Taufiq Rahman, Barry V. L. Potter, Colin W. Taylor
Summary: Inositol 1,4,5-trisphosphate receptors (IP(3)Rs) are intracellular Ca2+ channels linking extracellular stimuli to Ca2+ signals. Research shows that quantal responses to low IP3 concentrations do not require heterogeneous Ca2+ stores, and IP(3)Rs exhibit incremental responses to IP3 stimulation. This suggests that Ca2+ signals evoked by IP3 pulses arise from rapid activation and inactivation of a small fraction of IP(3)Rs.
Article
Biochemistry & Molecular Biology
Tomoya Uchimura, Hidetoshi Sakurai
Summary: The study showed that STIM1-Orai1 is a molecular target of store-operated Ca2+ channels (SOCs) and modulating its activity effectively ameliorated the decline in contractile performance in DMD.SOCs play a major role in causing dystrophin deficiency-dependent Ca2+ overload, and targeting STIM1-Orai1-mediated Ca2+ overload is an effective approach to rescuing contractile phenotypes in DMD.
Article
Multidisciplinary Sciences
Navid Paknejad, Vinay Sapuru, Richard K. Hite
Summary: IP3R receptors are endoplasmic reticulum Ca2+ channels that exhibit biphasic dependence on cytosolic Ca2+, leading to Ca2+ oscillations that regulate various cellular processes. This study reveals that Ca2+ binding does not induce explicit conformational changes, but biases a complex conformational landscape of the receptor. High-affinity Ca2+ binding allows the receptor to escape a low-energy resting state and activate through a series of preactivated states. At high Ca2+ concentrations, the receptor enters an inhibited state stabilized by a low-affinity Ca2+ binding site.
NATURE COMMUNICATIONS
(2023)
Article
Biophysics
Victor Nicolai Friedhoff, Benjamin Lindner, Martin Falcke
Summary: This article investigates the randomness and properties of Ca2+ signaling in cells. The researchers found that the generation of Ca2+ spikes follows certain patterns and can be described as a process from one state to another. They proposed a theoretical model to explain the differences in spike behavior between different cell types and stimulating agonists, as well as the variability observed in experiments.
BIOPHYSICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Joseph Minicucci, Molly Alfond, Angelo Demuro, David Gerberry, Joe Latulippe
Summary: This study developed a mathematical model describing a proposed mechanism by which iA beta Os stimulate PLC to produce IP3, leading to abnormal release of Ca2+. The research found a dose-dependent influence of iA beta Os on IP3-mediated Ca2+ signaling, and also revealed the impact of IP3 production on A beta-driven solution patterns in a dose-dependent manner.
Article
Physics, Fluids & Plasmas
Irina Bashkirtseva, Lev Ryashko
Summary: The stochastic excitability of spiking oscillatory regimes in calcium kinetics is investigated using the Li-Rinzel conceptual model. The probabilistic mechanisms of noise-induced generation of large-amplitude oscillations in parametric zones are studied numerically and analytically. The phenomenon of coherence resonance is discussed, and a parametric statistical description of interspike intervals is applied. The role of confidence ellipses and separatrices in determining sub- and supercritical regions is demonstrated.
Article
Cell Biology
Sou Inagaki, Yoshiaki Suzuki, Keisuke Kawasaki, Rubii Kondo, Yuji Imaizumi, Hisao Yamamura
Summary: In this study, the role of mitochondrial fusion protein 2 (Mfn2) in the coupling of sarcoplasmic reticulum (SR) and mitochondria in vascular smooth muscle cells (VSMCs) was examined. It was found that Mfn2 is responsible for tethering mitochondria to SR, which regulates calcium signaling, ATP production, and cell proliferation in VSMCs.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Valerio Farfariello, Dmitri Gordienko, Lina Mesilmany, Yasmine Touil, Emmanuelle Germain, Ingrid Fliniaux, Emilie Desruelles, Dimitra Gkika, Morad Roudbaraki, George Shapovalov, Lucile Noyer, Mathilde Lebas, Laurent Allart, Nathalie Zienthal-Gelus, Oksana Iamshanova, Franck Bonardi, Martin Figeac, William Laine, Jerome Kluza, Philippe Marchetti, Bruno Quesnel, Daniel Metzger, David Bernard, Jan B. Parys, Loic Lemonnier, Natalia Prevarskaya
Summary: This study reveals the mechanism of how alterations in mitochondrial function and calcium signaling contribute to cellular senescence and tumor growth. The downregulation of TRPC3 protein in senescent fibroblasts leads to increased mitochondrial calcium load and oxidative phosphorylation, promoting cellular senescence. The downregulation of TRPC3 in stromal cells also affects secretome and tumor progression, highlighting its importance in cancer development.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Kana Aoki, Shota Harada, Keita Kawaji, Kenji Matsuzawa, Seiichi Uchida, Junichi Ikenouchi
Summary: The cytoplasm in mammalian cells is considered homogeneous. Here authors report that the cytoplasmic fluidity is regulated in the blebbing cells, which is regulated by calcium concentration in the expanding blebs and involves the STIM-Orai1 pathway.
NATURE COMMUNICATIONS
(2021)