Article
Virology
Xiaohui Zhao, Huijun Yuan, Hang Yang, Yan Liu, Meng Xun, Xiaozhen Li, Tingting Fan, Bo Wu, Shangrui Guo, Hongliang Wang
Summary: EV71 is a common pathogen causing hand, foot, and mouth disease in young children, and it is associated with severe neurological complications. This study found that the ER-resident acetyltransferase NAT8 is a host factor for EV71 infection and promotes viral replication by interacting with viral proteins.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Namasthee Harris-Gauthier, Annika Traa, Abdelrahman AlOkda, Alibek Moldakozhayev, Ulrich Anglas, Sonja K. Soo, Jeremy M. Van Raamsdonk
Summary: Mild impairment of mitochondrial function can increase lifespan in genetic model organisms. The upregulation of genes involved in the mitochondrial thioredoxin system, mediated by the mitochondrial unfolded protein response, is specifically required for the longevity of mitochondrial mutants. The mitochondrial thioredoxin system also plays a role in stress resistance.
Article
Medicine, Research & Experimental
Jean Bastin, Marine Sroussi, Ivan Nemazanyy, Pierre Laurent-Puig, Sophie Mouillet-Richard, Fatima Djouadi
Summary: This study investigated the expression and function of mitochondrial Complex I (CI) in different molecular subtypes of colorectal cancer (CRC), and found that CI is downregulated in CMS4 subtype, which is associated with higher mitochondrial ROS production. The study also revealed that promoting or scavenging mitochondrial ROS mitigates migration of CMS4 cells. Furthermore, clinical data showed that downregulated CI expression in CMS4 subtype is associated with poor prognosis.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Neurosciences
Tingting Yan, Yan Zhao, Zhongyu Jiang, Jiyang Chen
Summary: This study investigated the role of mitophagy in cytotoxicity induced by acetaldehyde, a toxic metabolite of ethanol. The findings showed that acetaldehyde treatment induced mitophagy and cytotoxicity in SH-SY5Y cells. The overactive mitophagy and oxidative stress were identified as important mechanisms contributing to acetaldehyde-induced cytotoxicity. Autophagy inhibitors and an antioxidant could alleviate the cytotoxic effects.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Jyoti Tanwar, Suman Saurav, Reelina Basu, Jaya Bharti Singh, Anshu Priya, Maitreyee Dutta, Uma Santhanam, Manoj Joshi, Stephen Madison, Archana Singh, Nirmala Nair, Rajesh S. Gokhale, Rajender K. Motiani
Summary: Inter-organellar communication plays a crucial role in regulating cellular physiology, and MFN2 is a key regulator involved in this process. Our study reveals that MFN2 negatively regulates melanogenesis, and its knockout leads to enhanced melanin synthesis, possibly through the modulation of mitochondrial ROS levels.
Article
Biochemistry & Molecular Biology
Chih-Hsing Hung, Yi-Ching Lin, Yi-Giien Tsai, Yu-Chih Lin, Chia-Hong Kuo, Mei-Lan Tsai, Chao-Hung Kuo, Wei-Ting Liao
Summary: Acrylamide promoted mitochondria-specific ROS generation, increased gene expression of mitochondrial respiratory chain complex II SDHA, induced histone modifications and upregulated PINK1 expression, leading to a macrophage M2 polarization shift in THP-1 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Chunchen Wu, Luzhi Zeng, Wenfu Yi, Yuanjiu Miao, Yihan Liu, Qiming Wang, Shi Liu, Guoping Peng, Zhenhua Zheng, Jianbo Xia
Summary: This study demonstrates that echovirus infection can induce autophagy, which promotes viral replication and VP1 expression in host cells. The mechanism involves the regulation of the mTOR/ULK1 signaling pathway, providing insight into the potential role of autophagy in echovirus infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Jaskaran Kaur, Yogita Rawat, Vikas Sood, Neha Periwal, Deepak Kumar Rathore, Shrikant Kumar, Niraj Kumar, Sankar Bhattacharyya
Summary: In this study, it was found that Dengue virus can infect human megakaryocytes and suppress platelet biogenesis. Differentiating megakaryocyte cells were shown to support higher viral replication, while the virus did not increase its entry into the cells. Dengue replication inhibited the accumulation of intracellular reactive oxygen species (ROS) by enhancing the activity of the transcription factor NFE2L2. Interestingly, modulation of NFE2L2 activity had opposite effects on intracellular ROS and virus replication. Additionally, differentiated cells with intracellular virus replication showed reduced levels of surface markers compared to uninfected differentiated cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hyun-Jung Park, Sun-Young Yoon, Jung-Nam Park, Jae-Hee Suh, Hye-Seon Choi
Summary: This study found that doxorubicin-induced bone loss is partly caused by the upregulation of autophagy in osteoclasts (OCs). Doxorubicin induces autophagy in OCs through the mitochondrial reactive oxygen species (mROS)/transient receptor potential mucolipin 1 (TRPML1)/transcription factor EB (TFEB) axis pathway. The results show that doxorubicin-induced autophagy increases the area of OCs and bone resorption activity, while decreasing the number of OCs through apoptosis, resulting in bone loss. The autophagy inhibitor 3-methyladenine (3-MA) reverses the effects of doxorubicin by reducing the area of OCs, thus reversing doxorubicin-induced bone loss.
Article
Virology
Wanling Zhang, Haiyan Yang, Zhengyun Liu, Shengyu Wang, Tianyang Chen, Hong Song, Yunbin Xu, Fajin Li, Guo Luo, Huan Wang
Summary: EV71 infection leads to changes in mitochondrial morphology and dynamics in human neuroblastoma cells, resulting in mitochondrial dysfunction that may contribute to nervous system dysfunction.
Article
Pharmacology & Pharmacy
Da Eun Lee, Hyeon Woong Kang, So Yi Kim, Myeong Jin Kim, Jae Woong Jeong, Woosol Chris Hong, Sungsoon Fang, Hyung Sun Kim, Yun Sun Lee, Hyo Jung Kim, Joon Seong Park
Summary: This study demonstrates that the combination of ivermectin and gemcitabine effectively suppresses pancreatic cancer by inhibiting cell proliferation and inducing cell apoptosis through cell cycle arrest and mitochondrial dysfunction. In vivo experiments also support the tumor-inhibiting effect of this combination treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Andrey Y. Y. Vinokurov, Vladislav O. O. Soldatov, Evgenia S. S. Seregina, Angelina I. I. Dolgikh, Pavel A. A. Tagunov, Andrey V. V. Dunaev, Marina Y. Y. Skorkina, Alexey V. V. Deykin, Andrey Y. Y. Abramov
Summary: Alterations in function of HPRT lead to overproduction of uric acid and symptoms of LNS. HPRT1 deficiency inhibits mitochondrial respiration and increases ROS production, but does not induce oxidative stress or decrease the level of antioxidant glutathione.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Plant Sciences
Yuqin Zhang, Yuanyuan Hu, Zhiqin Wang, Xiaodi Lin, Zihui Li, Yafang Ren, Jie Zhao
Summary: This study investigates the biological functions of AtTIM22-2 in Arabidopsis. Loss of AtTIM22-2 function leads to defects in embryo and endosperm development, resulting in seed abortion. AtTIM22-2 interacts with AtTIM23-2, AtB14.7, and AT5G27395, participating in mitochondrial biogenesis and maintaining mitochondrial functions and ROS homeostasis.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Medicine, Research & Experimental
Xiaoyan Zhang, Jinfang Hao, Chenxi Sun, Jianping Du, Qian Han, Qingshan Li
Summary: Total astragalosides (ASTs) from Astragalus membranaceus have a protective effect against EV71 infection by inhibiting cell apoptosis and pyroptosis, as well as suppressing EV71 replication and release.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2022)
Article
Cell Biology
Yanhong Wang, Xinyu Mang, Xuran Li, Zhengyu Cai, Fei Tan
Summary: This study investigated the effect and mechanism of cold atmospheric plasma (CAP) on the proliferation and apoptosis of lung cancer and colon cancer cells. The results showed that CAP treatment inhibited tumor cell proliferation and induced apoptosis through the activation of the mitochondrial pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Allergy
Chih-Yung Chiu, Mei-Ling Cheng, Meng-Han Chiang, Yu-Lun Kuo, Ming-Han Tsai, Chun-Che Chiu, Gigin Lin
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2019)
Article
Medicine, General & Internal
Huan-Tang Lin, Mei-Ling Cheng, Chi-Jen Lo, Gigin Lin, Shu-Fu Lin, Jiun-Ting Yeh, Hung-Yao Ho, Jr-Rung Lin, Fu-Chao Liu
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Yu-Chieh Huang, Mei-Ling Cheng, Hsiang-Yu Tang, Chi-Yao Huang, Kuan-Ming Chen, Jong-Shyan Wang
Summary: This study explores the impact of eccentric cycling training (ECT) on erythrocyte antioxidative capacity and metabolism in sedentary males. The results indicate that ECT enhances antioxidative capacity, promotes oxygen release, and mitigates oxidative stress induced by exercise, suggesting the beneficial effects of ECT on overall health and performance.
Article
Biochemistry & Molecular Biology
Ying-Chieh Lai, Ching-Yi Hsieh, Kuan-Ying Lu, Cheng-Hsuan Sung, Hung-Yao Ho, Mei-Ling Cheng, Albert P. Chen, Shu-Hang Ng, Fang-Hsin Chen, Gigin Lin
Summary: The study utilized a new imaging technique, DNP C-13-MRI, to explore the metabolic effects of radiation on cancer cells and immune cells. The results showed significant changes in glycolytic flux rates in different types of cells after irradiation, suggesting the potential clinical utility of DNP C-13-MRI for future treatments.
Article
Cell Biology
Chia-Ni Lin, Ming-Shi Shiao, Mei-Ling Cheng, Chiung-Mei Chen, Hung-Chou Kuo
Summary: This study aimed to present serum metabolite profiles of patients with Acute Intermittent Porphyria (AIP) and identify specific metabolites that could discriminate between AIP, asymptomatic HMBS mutation carriers, and healthy individuals. The results showed significant differences in 15 variables between AIP patients and normal controls, suggesting potential biomarkers for monitoring AIP symptoms.
Article
Cell Biology
Kuo-Hsuan Chang, Mei-Ling Cheng, Hsiang-Yu Tang, Cheng-Yu Huang, Hsiu-Chuan Wu, Chiung-Mei Chen
Summary: Using targeted metabolomics analysis, this study identified new blood biomarker candidates for Parkinson's disease (PD) and found significant correlations between these biomarkers and disease progression and clinical manifestations of PD.
Article
Cell Biology
Mei-Ling Cheng, Hsiang-Yu Tang, Pei-Ting Wu, Cheng-Hung Yang, Chi-Jen Lo, Jui-Fen Lin, Hung-Yao Ho
Summary: 7-Ketocholesterol induces changes in lipid metabolism in cardiac cells, leading to alterations in levels of lipid substances and particularly enhancing cholesteryl ester accumulation. Transcriptomic analysis reveals differential expression of genes involved in lipid metabolic processes in treated cells, suggesting a reprogramming of lipid metabolism through altered gene transcription may be the underlying mechanism.
Article
Biochemistry & Molecular Biology
Ying-Chang Tung, Mei-Ling Cheng, Lung-Sheng Wu, Hsiang-Yu Tang, Cheng-Yu Huang, Gwo-Jyh Chang, Chi-Jen Chang
Summary: This study investigates the changes and reversibility of cardiac energetics in a rat model of cardiac volume overload (VO) using transcriptomics and metabolomics analyses. The findings suggest that glycolysis is downregulated and redirected towards the hexosamine biosynthetic pathway (HBP) and O-linked-N-acetylglucosaminylation in the compensated hypertrophy (CH) phase, and further suppressed in the progression to heart failure (HF). Derangements in fatty acid oxidation become prominent only in HF. Correction of VO can reverse the differential expression of genes involved in glycolysis, HBP, and fatty acid oxidation in CH but not in HF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Kuan-Hsing Chen, Li-Fang Chou, Cheng-Chieh Hung, Hsiang-Yu Tang, Mei-Ling Cheng, Huang-Yu Yang, Hsiang-Hao Hsu, Ya-Chung Tian, Chih-Wei Yang
Summary: Analysis of murine model data revealed that urinary levels of L-carnitine and acetyl-L-carnitine were significantly elevated in L. interrogans-infected mice, involved in multiple metabolic pathways, suggesting their potential as metabolic modulators in leptospiral infection-induced kidney injury.
Article
Pharmacology & Pharmacy
Yueh-Hsiang Huang, Yi-Hong Wu, Hsiang-Yu Tang, Szu-Tah Chen, Chih-Ching Wang, Wan-Jing Ho, Yi-Hsuan Lin, Geng-Hao Liu, Pei-Yeh Lin, Chi-Jen Lo, Yuan-Ming Yeh, Mei-Ling Cheng
Summary: This study found that the concentrated herbal extract formula YH1 improved blood glucose and lipid control in patients with type 2 diabetes and hyperlipidemia by modulating microbiota constituents and altering fecal and plasma bile acid compositions.
Article
Biochemistry & Molecular Biology
Li-Chung Chiu, Hsiang-Yu Tang, Chun-Ming Fan, Chi-Jen Lo, Han-Chung Hu, Kuo-Chin Kao, Mei-Ling Cheng
Summary: Acute respiratory distress syndrome (ARDS) is characterized by dysregulated immune-inflammatory responses and high mortality. This study found that the activation of the kynurenine pathway, a metabolic pathway, is associated with mortality in ARDS patients. Plasma kynurenine levels and the kynurenine/tryptophan ratio were higher in non-survivors compared to survivors. Urine metabolic profiling also revealed higher tryptophan degradation in non-survivors. These findings suggest that the kynurenine pathway could serve as a prognostic marker and a target for therapeutic interventions in ARDS.
Article
Medicine, General & Internal
Huan-Tang Lin, Mei-Ling Cheng, Chi-Jen Lo, Gigin Lin, Fu-Chao Liu
Summary: The study identifies altered metabolic markers in the cerebrospinal fluid and plasma of diabetic patients with DKD, which could be valuable for monitoring the progression of DKD.
Article
Biochemistry & Molecular Biology
Mei-Ling Cheng, Cheng-Hung Yang, Pei-Ting Wu, Yi-Chin Li, Hao-Wei Sun, Gigin Lin, Hung-Yao Ho
Summary: 7-Ketocholesterol (7KCh), the major oxidized product of cholesterol, induces cellular oxidative damage. This study investigated the physiological responses of cardiomyocytes to 7KCh. Treatment with 7KCh inhibited the growth of cardiac cells and their mitochondrial oxygen consumption, while also resulting in compensatory increase in mitochondrial mass and adaptive metabolic remodeling. The accumulation of malonyl-CoA was found to play a role in the growth inhibitory effect of 7KCh.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hsiang-Yu Tang, Jyh-En Huang, Ming-Tong Tsau, Chi-Jen Chang, Ying-Chang Tung, Gigin Lin, Mei-Ling Cheng
Summary: Volume overload and heart failure have an impact on metabolic changes in the kidney, including purine metabolism, taurine metabolism, glycine, serine, and threonine metabolism, glycerophospholipid metabolism, and histidine metabolism. Metabolic profiles are more sensitive than clinical parameters in responding to kidney damage in HF.
Article
Biochemistry & Molecular Biology
Hsiang-Yu Tang, Chao-Hung Wang, Hung-Yao Ho, Jui-Fen Lin, Chi-Jen Lo, Cheng-Yu Huang, Mei-Ling Cheng