Article
Plant Sciences
Miroslav Ovecka, Jiri Sojka, Michaela Ticha, George Komis, Jasim Basheer, Cintia Marchetti, Olga Samajova, Lenka Kubenova, Jozef Samaj
Summary: The documentation of plant growth and development requires integrative and scalable approaches. Recent developments in mesoscopy, microscopy and nanoscopy methods have been applied to imaging of plant subcellular compartments, cells, tissues, and organs. However, the current LSFM and SRM modalities have limitations and there is a trend towards designing integrated microscopic modalities for broader multiscale plant imaging.
Article
Multidisciplinary Sciences
Caio Vaz Rimoli, Cesar Augusto Valades-Cruz, Valentina Curcio, Manos Mavrakis, Sophie Brasselet
Summary: Single-molecule localization microscopy does not provide orientation information of proteins in cells. The authors propose a method called 4polar-STORM combining STORM with four-polarisation image splitting to measure single molecule orientation and wobbling in cells.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yicong Wu, Xiaofei Han, Yijun Su, Melissa Glidewell, Jonathan S. Daniels, Jiamin Liu, Titas Sengupta, Ivan Rey-Suarez, Robert Fischer, Akshay Patel, Christian Combs, Junhui Sun, Xufeng Wu, Ryan Christensen, Corey Smith, Lingyu Bao, Yilun Sun, Leighton H. Duncan, Jiji Chen, Yves Pommier, Yun-Bo Shi, Elizabeth Murphy, Sougata Roy, Arpita Upadhyaya, Daniel Colon-Ramos, Patrick La Riviere, Hari Shroff
Summary: Researchers have enhanced the performance of confocal microscopy using four approaches: developing compact line scanners, combining multi-view imaging, adapting techniques from structured illumination microscopy, and utilizing deep learning. They successfully improved imaging resolution, reduced phototoxicity, and demonstrated these advancements on over 20 different samples.
Review
Spectroscopy
Shawn Yoshida, Lydia Kisley
Summary: The extracellular matrix (ECM) is a crucial biophysical environment involved in physiological processes. Super-resolution fluorescence microscopy offers the potential to investigate local, nanoscale, physicochemical variations in the ECM, providing insights into molecular-level ECM processes and guiding future biomedical research directions.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biotechnology & Applied Microbiology
Kanuj Mishra, Juan Pablo Fuenzalida-Werner, Francesca Pennacchietti, Robert Janowski, Andriy Chmyrov, Yuanhui Huang, Christian Zakian, Uwe Klemm, Ilaria Testa, Dierk Niessing, Vasilis Ntziachristos, Andre C. Stiel
Summary: Reversibly photo-switchable proteins have been utilized for super-resolution and optoacoustic imaging methods, and in this study, a prototype of a photo-switchable Ca2+ sensor was constructed based on GCaMP5G. The molecular mechanisms of the sensor were described at the structural level, demonstrating the ability to image calcium and other analytes at super-resolution and in vivo.
NATURE BIOTECHNOLOGY
(2022)
Article
Cell Biology
Yun-Bin Lee, Minkyo Jung, Jeesoo Kim, Afandi Charles, Wanda Christ, Jiwoong Kang, Myeong-Gyun Kang, Chulhwan Kwak, Jonas Klingstrom, Anna Smed-Sorensen, Jong-Seo Kim, Ji Young Mun, Hyun-Woo Rhee
Summary: This study used a super-resolution proximity labeling (SR-PL) method to investigate the interactions between SARS-CoV-2 and host cells. The results showed that RNF5 interacts with ORF3a and promotes its protein degradation, leading to a reduction in SARS-CoV-2 infection rate.
Article
Chemistry, Multidisciplinary
Hongru Li, Jinrui Zhang, Yan Shi, Guanfang Zhao, Haijiao Xu, Mingjun Cai, Jing Gao, Hongda Wang
Summary: This study uses super-resolution imaging to reveal the distribution changes of the insulin receptor during cell membrane activation. The findings show that insulin resistance inhibits receptor clustering and there is a highly co-localized relationship between the insulin receptor and the skeletal protein beta II-spectrin.
Article
Biophysics
Jianan Dai, Zihan Wu, Di Li, Guishan Peng, Guannan Liu, Ri Zhou, Chenguang Wang, Xu Yan, Fangmeng Liu, Peng Sun, Ji Zhou, Geyu Lu
Summary: A new fluorescent probe called Lipi-Deep Red has been developed for super-resolution imaging of lipid droplets (LDs), allowing for visualization and study of LDs dynamics and interaction with mitochondria at the nanoscale level. This probe offers strong deep red/NIR emission, high LDs specificity, and excellent photostability, enabling precise monitoring and visualization of LD fusion processes and the discovery of two contact models between LDs and mitochondria.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
G. M. Jonaid, William J. Dearnaley, Michael A. Casasanta, Liam Kaylor, Samantha Berry, Madeline J. Dukes, Michael S. Spilman, Jennifer L. Gray, Deborah F. Kelly
Summary: Liquid-phase electron microscopy (LP-EM) is a new exciting field in materials imaging, offering unprecedented views of molecular processes. Insights from LP-EM studies complement other high-resolution techniques, and expanding this technology into 3D realm presents future opportunities.
ADVANCED MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Nicole C. Robb
Summary: Diseases caused by viruses, such as the COVID-19 pandemic, pose significant challenges to human society. Super-resolution imaging technologies have enabled the direct visualization of virus structures at the nanoscale, providing new insights into their morphology and structure.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Koen van den Dries, Jack Fransen, Alessandra Cambi
Summary: Correlative light and electron microscopy (CLEM) is a powerful imaging approach that combines light and electron microscopy, and has wide applications in biology. This review summarizes the important developments in CLEM for biological research, with a focus on the combination of fluorescence microscopy and electron microscopy.
Article
Chemistry, Physical
Milan Vala, Lukasz Bujak, Antonio Garcia Marin, Kristyna Holanova, Verena Henrichs, Marcus Braun, Zdenek Lansky, Marek Piliarik
Summary: Research reveals that microtubule disassembly is a discrete process, suggesting a stochastic mechanism of switching from disassembly to assembly phase.
Review
Optics
Mingwei Tang, Yubing Han, Danchen Jia, Qing Yang, Ji-Xin Cheng
Summary: Far-field chemical microscopy, with molecular electronic or vibrational fingerprint information, offers a new perspective for studying three-dimensional biological, material, and chemical systems. Despite the limitation of optics, recent advances in super-resolution techniques have overcome this hurdle, pushing the boundaries of far-field chemical microscopy in terms of spatial resolution. This technique finds applications in various fields including biomedical research, material characterization, environmental study, cultural heritage conservation, and integrated chip inspection.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Biochemical Research Methods
Linjing Fang, Fred Monroe, Sammy Weiser Novak, Lyndsey Kirk, Cara R. Schiavon, Seungyoon B. Yu, Tong Zhang, Melissa Wu, Kyle Kastner, Alaa Abdel Latif, Zijun Lin, Andrew Shaw, Yoshiyuki Kubota, John Mendenhall, Zhao Zhang, Gulcin Pekkurnaz, Kristen Harris, Jeremy Howard, Uri Manor
Summary: Point-scanning imaging systems are widely used for high-resolution cellular and tissue imaging, but optimizing resolution, speed, sample preservation, and signal-to-noise ratio simultaneously is challenging. The use of deep learning-based supersampling, known as point-scanning super-resolution (PSSR) imaging, can mitigate these limitations. PSSR facilitates high-resolution, fast, and sensitive image acquisition with otherwise unattainable resolution.
Article
Gastroenterology & Hepatology
Lena Allweiss, Sofia Gass, Katja Giersch, Anne Groth, Janine Kah, Tassilo Volz, Gianna Rapp, Anja Schoebel, Ansgar W. Lohse, Susanne Polywka, Sven Pischke, Eva Herker, Maura Dandri, Marc Luetgehetmann
JOURNAL OF HEPATOLOGY
(2016)
Article
Multidisciplinary Sciences
Paula Ruibal, Lisa Oestereich, Anja Luedtke, Beate Becker-Ziaja, David M. Wozniak, Romy Kerber, Misa Korva, Mar Cabeza-Cabrerizo, Joseph A. Bore, Fara Raymond Koundouno, Sophie Duraffour, Romy Weller, Anja Thorenz, Eleonora Cimini, Domenico Viola, Chiara Agrati, Johanna Repits, Babak Afrough, Lauren A. Cowley, Didier Ngabo, Julia Hinzmann, Marc Mertens, Ines Vitoriano, Christopher H. Logue, Jan Peter Boettcher, Elisa Pallasch, Andreas Sachse, Amadou Bah, Katja Nitzsche, Eeva Kuisma, Janine Michel, Tobias Holm, Elsa-Gayle Zekeng, Isabel Garcia-Dorival, Roman Woelfel, Kilian Stoecker, Erna Fleischmann, Thomas Strecker, Antonino Di Caro, Tatjana Avsic-Zupanc, Andreas Kurth, Silvia Meschi, Stephane Mely, Edmund Newman, Anne Bocquin, Zoltan Kis, Anne Kelterbaum, Peter Molkenthin, Fabrizio Carletti, Jasmine Portmann, Svenja Wolff, Concetta Castilletti, Gordian Schudt, Alexandra Fizet, Lisa J. Ottowell, Eva Herker, Thomas Jacobs, Birte Kretschmer, Ettore Severi, Nobila Ouedraogo, Mar Lago, Anabel Negredo, Leticia Franco, Pedro Anda, Stefan Schmiedel, Benno Kreuels, Dominic Wichmann, Marylyn M. Addo, Ansgar W. Lohse, Hilde De Clerck, Carolina Nanclares, Sylvie Jonckheere, Michel Van Herp, Armand Sprecher, Gao Xiaojiang, Mary Carrington, Osvaldo Miranda, Carlos M. Castro, Martin Gabriel, Patrick Drury, Pierre Formenty, Boubacar Diallo, Lamine Koivogui, N'Faly Magassouba, Miles W. Carroll, Stephan Guenther, Cesar Munoz-Fontela
Article
Cell Biology
Kathrin Roesch, Marcel Kwiatkowski, Sarah Hofmann, Anja Schoebel, Cordula Gruettner, Marcus Wurlitzer, Hartmut Schlueter, Eva Herker
Article
Multidisciplinary Sciences
Kathrin Roesch, Marcel Kwiatkowski, Hartmut Schlueter, Eva Herker
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2017)
Article
Biochemistry & Molecular Biology
Sarah Hofmann, Matthias Krajewski, Christina Scherer, Verena Scholz, Valerie Mordhorst, Pavel Truschow, Anja Schoebel, Rudolph Reimer, Dominik Schwudke, Eva Herker
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2018)
Article
Gastroenterology & Hepatology
Sebastian Lunemann, Anja Schoebel, Janine Kah, Pia Fittje, Angelique Hoelzemer, Annika E. Langeneckert, Leonard U. Hess, Tobias Poch, Gloria Martrus, Wilfredo F. Garcia-Beltran, Christian Koerner, Annerose E. Ziegler, Laura Richert, Karl J. Oldhafer, Julian Schulze zur Wiesch, Christoph Schramm, Maura Dandri, Eva Herker, Marcus Altfeld
Article
Multidisciplinary Sciences
Anja Schoebel, Kathrin Roesch, Eva Herker
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Susan Lassen, Cordula Gruettner, Van Nguyen-Dinh, Eva Herker
JOURNAL OF CELL SCIENCE
(2019)
Review
Biochemistry & Molecular Biology
Hanna Bley, Anja Schoebel, Eva Herker
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Cell Biology
Mathias Beller, Eva Herker, Joachim Fuellekrug
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2020)
Article
Gastroenterology & Hepatology
Thomas Horvatits, Jan-Erik Wissmann, Reimar Johne, Martin H. Groschup, Ashish K. Gadicherla, Julian Schulze Zur Wiesch, Martin Eiden, Daniel Todt, Rudolph Reimer, Lisa Daehnert, Anja Schoebel, Karoline Horvatits, Rabea Luebke, Christine Wolschke, Francis Ayuk, Meike Rybczynski, Ansgar W. Lohse, Marylyn M. Addo, Eva Herker, Marc Luetgehetmann, Eike Steinmann, Sven Pischke
Summary: The study found HEV-RNA in the seminal plasma and semen of chronically infected patients, with viral loads >2 logs higher than in the serum, indicating the male reproductive system as a niche of HEV persistence in chronic infection. Additionally, HEV particles in the ejaculate did not morphologically differ from serum-derived particles, suggesting a potential route for HEV transmission.
JOURNAL OF HEPATOLOGY
(2021)
Article
Cell Biology
Eva Herker, Gabrielle Vieyres, Mathias Beller, Natalie Krahmer, Maria Bohnert
Summary: Lipid droplets, once seen as inert fat drops, are now recognized as vital organelles with key functions in lipid biology and cellular communication. Diseases factors mutated in hereditary disorders have been linked to LD contact site proteins, indicating the importance of LD-organelle contacts in disease development and progression. Additionally, LD contact sites are dynamically rearranged in response to infections and pathological metabolic conditions, suggesting their crucial role in disease pathways.
TRENDS IN CELL BIOLOGY
(2021)
Article
Microbiology
Anja Schobel, Van Nguyen-Dinh, Gerald G. Schumann, Eva Herker
Summary: HCV infection increases levels of endogenous L1 protein and restricts retrotransposition of engineered L1 reporter elements by inducing stress granules.
Review
Cell Biology
Van Nguyen-Dinh, Eva Herker
Summary: Intracellular pathogens rely heavily on host cell organelles and metabolites for successful infection and replication. Positive-strand RNA viruses often induce alterations to the membrane system to shield their replication intermediates and accumulate necessary factors for efficient replication, while also preventing antiviral effectors from accessing. The specific structure and source of membranes induced by these viruses differ in plant and animal cells, but the principle remains consistent across species.
Review
Cell Biology
Didac Carmona-Gutierrez, Maria Anna Bauer, Andreas Zimmermann, Andres Aguilera, Nicanor Austriaco, Kathryn Ayscough, Rena Balzan, Shoshana Bar-Nun, Antonio Barrientos, Peter Belenky, Marc Blondel, Ralf J. Braun, Michael Breitenbach, William C. Burhans, Sabrina Buttner, Duccio Cavalieri, Michael Chang, Katrina F. Cooper, Manuela Corte-Real, Vitor Costa, Christophe Cullin, Ian Dawes, Jorn Dengjel, Martin B. Dickman, Tobias Eisenberg, Birthe Fahrenkrog, Nicolas Fasel, Kai-Uwe Frohlich, Ali Gargouri, Sergio Giannattasio, Paola Goffrini, Campbell W. Gourlay, Chris M. Grant, Michael T. Greenwood, Nicoletta Guaragnella, Thomas Heger, Juergen Heinisch, Eva Herker, Johannes M. Herrmann, Sebastian Hofer, Antonio Jimenez-Ruiz, Helmut Jungwirth, Katharina Kainz, Dimitrios P. Kontoyiannis, Paula Ludovico, Stephen Manon, Enzo Martegani, Cristina Mazzoni, Lynn A. Megeney, Chris Meisinger, Jens Nielsen, Thomas Nystrom, Heinz D. Osiewacz, Tiago F. Outeiro, Hay-Oak Park, Tobias Pendl, Dina Petranovic, Stephane Picot, Peter Polcic, Ted Powers, Mark Ramsdale, Mark Rinnerthaler, Patrick Rockenfeller, Christoph Ruckenstuhl, Raffael Schaffrath, Maria Segovia, Fedor F. Severin, Amir Sharon, Stephan J. Sigrist, Cornelia Sommer-Ruck, Maria Joao Sousa, Johan M. Thevelein, Karin Thevissen, Vladimir Titorenko, Michel B. Toledano, Mick Tuite, F. -Nora Voegtle, Benedikt Westermann, Joris Winderickx, Silke Wissing, Stefan Woelfl, Zhaojie J. Zhang, Richard Y. Zhao, Bing Zhou, Lorenzo Galluzzi, Guido Kroemer, Frank Madeo
