Review
Chemistry, Analytical
Piliang Xiang, Yu Yang, Huang Chen, Apeng Chen, Shaorong Liu
Summary: Significant progresses have been made recently in liquid chromatography using <= 5-mu m-i.d. open tubular columns, enabling peak capacities of 2000+, efficiencies of 10+ million plates/m, and millisecond separations. This review article summarizes the latest advancements, procedures for preparing columns, selecting experimental components, and discusses the future prospects of this technique.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Natalia G. P. Santos, Deyber A. V. Medina, Fernando M. Lancas
Summary: This study explores the application of WCOT column in LC-MS/MS. The column preparation process encompasses column pretreatment, silanization, stationary phase coating, and immobilization. Various parameters that may affect column efficiency, including stationary phase composition, length, internal diameter, mass, and injection volume, are evaluated. The optimized WCOT column, with a length of 8 m, an inner diameter of 25 μm, OV-210 mass of 2.5% m/v, and injection volume of 100 nL, coupled with a commercial trapping column in the nanoLC system, demonstrates improved separation resolution.
Article
Chemistry, Analytical
Aaron James F. Reyes, Reta Birhanu Kitata, Mira Anne C. dela Rosa, Yi-Ting Wang, Pei-Yi Lin, Pan-Chyr Yang, Assaf Friedler, Shlomo Yitzchaik, Yu-Ju Chen
Summary: The dysregulation of phosphorylation-mediated signaling plays a critical role in the development of diseases. A sensitive and rapid substrate-specific kinase assay has been developed using site-specific peptide reporter and MRM-MS platform, allowing for precise monitoring of disease states. This approach offers a rapid and sensitive method for targeted quantification of substrate-specific kinase activity in small amounts of samples, with high accuracy and reproducibility.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Kendall R. Johnson, Michal Gregus, Alexander R. Ivanov
Summary: In this study, the combination of high-field asymmetric waveform ion mobility spectrometry (FAIMS) with ultrasensitive capillary electrophoresis hyphenated with tandem mass spectrometry (CE-MS/MS) was used for deep proteome coverage of low nanogram amounts of digested cell lysates. The optimized CE-FAIMS-MS/MS method showed significant improvements in protein and peptide identification, as well as detection of multiphosphorylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Analytical
Wei Hu, Wei Zhou, Chenlu Wang, Zichun Liu, Zilin Chen
Summary: The development of a high-throughput and rapid screening analytical method is in high demand for anti-doping and clinical point-of-care analysis. This study developed an online direct coupling in-tube solid-phase microextraction-mass spectrometry method, which proved to be effective for target analysis in complex sample matrixes.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Fujia Wang, Tim Veth, Marije Kuipers, Maarten Altelaar, Kelly E. Stecker
Summary: A successful mass spectrometry-based phosphoproteomics analysis relies on effective sample preparation strategies. The performance of the S-Trap protocol for phosphoproteomics studies is unclear, and the addition of phosphoric acid (PA) in the existing protocol is detrimental to downstream phosphopeptide enrichment. This study systematically evaluates the performance of the S-Trap digestion for proteomics and phosphoproteomics, and demonstrates that an optimized S-Trap approach, with trifluoroacetic acid substituted for PA, is a simple and effective method for phosphoproteomics sample preparation.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Claudia Venditti, Valentina Biagioni, Alessandra Adrover, Stefano Cerbelli
Summary: The impact of transversal flow on separation resolution in Open-Tubular Liquid Chromatography (OTLC) is analyzed. The results show that the presence of transversal flows can reduce the dependence of column length on the Peclet number.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Azad Eshghi, Xiaofeng Xie, Darryl Hardie, Michael X. Chen, Fabiana Izaguirre, Rachael Newman, Ying Zhu, Ryan T. Kelly, David R. Goodlett
Summary: In this study, three cell isolation and two proteomic sample preparation methods were compared for single-cell and near single-cell analysis. The CellenONE device was the only method that could reliably isolate single cells and measure hemoglobin levels comparable to the calculated SI reference range. Additionally, using multiple red blood cells obtained from multiple blood donors proved to be a more efficient strategy for measuring protein heterogeneity compared to single-cell analysis.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Holly R. Smith, Emily H. Canessa, Runia Roy, Rita Spathis, Michel Shamoon Pour, Yetrib Hathout
Summary: This study used mass spectrometry to detect pathogen proteins in black-legged ticks, and found that these ticks carried various pathogens, including Lyme disease and Rickettsia. Analysis of individual ticks revealed the presence of different pathogens.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Pelin Yildiz, Sureyya Ozcan
Summary: Recent advances in proteomics technologies have allowed for high-throughput analysis of thousands of proteins. In this study, the correlation between protein concentration and corresponding unique peptide responses was investigated under conventional proteolytic digestion conditions. The results indicate that reproducible peptide signals could be misleading in clinical studies and that peptide selection could significantly impact the protein level outcome. This study is the first to explore quantitative protein-peptide correlations in biological samples using all unique peptides representing the same protein, and it opens up a discussion on peptide-based proteomics.
Article
Biochemistry & Molecular Biology
Paleerath Peerapen, Wanida Boonmark, Visith Thongboonkerd
Summary: In this study, the interacting complexes of the apical membrane (ApANXA1) and cytosolic (cyANXA1) forms of ANXA1 in renal epithelial cells under high-calcium condition were characterized using proteomic and bioinformatic approaches. The results showed that the interacting proteins had both common and unique characteristics and properties. This study provides insights into the biochemistry, functions, and pathophysiology of CaOx kidney stone formation induced by high-calcium condition.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemical Research Methods
Fanni Bugyi, Gabor Toth, Kinga Bernadett Kovacs, Laszlo Drahos, Lilla Turiak
Summary: Efficient phosphoproteomic analysis of small amounts of biological samples requires careful enrichment and purification steps. This study evaluated the performance of various self-packed and commercial centrifugal SPE cartridges/spin tips, and identified several methods that provided excellent results in terms of sample purification, reducing sample loss, and increasing the detection and recovery of phosphopeptides.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Alexey S. Kononikhin, Alexander G. Brzhozovskiy, Anna E. Bugrova, Natalia V. Chebotareva, Natalia V. Zakharova, Savva Semenov, Anatoliy Vinogradov, Maria I. Indeykina, Sergey Moiseev, Irina M. Larina, Evgeny N. Nikolaev
Summary: Targeted quantitative urine proteome analysis by mass spectrometry (MS) is a promising tool for early diagnosis of chronic kidney disease (CKD) and can replace invasive biopsy.
Article
Chemistry, Analytical
Yue Sun, Yu Liang, Chao Wang, Baofeng Zhao, Zhen Liang, Xiaodan Zhang, Yukui Zhang, Lihua Zhang
Summary: Comprehensive characterization of membrane proteins at the level of proteoforms in complex biological samples by top-down mass spectrometry (MS) is of vital importance in revealing their precise functions. However, severe peak broadening in the separation of hydrophobic membrane proteins, caused by resistance to mass transfer and strong adsorption on separation materials, leads to MS spectra overlap and signal suppression, which makes against the in-depth research on membrane proteoforms.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yingyan Zhou, Guangsheng Guo, Xiayan Wang
Summary: Chromatography theory suggests that ultranarrow-bore open tubular liquid chromatography holds advantages in small sample requirement and high separation efficiency, but also presents challenges. Research efforts aim to mature its application in ultra-small sample analysis like single-cell and subcellular organelles omics research.
CHINESE JOURNAL OF CHEMISTRY
(2022)
Article
Toxicology
Marianne Skov-Skov Bergh, Inger Lise Bogen, Elisabeth Nerem, Ariane Wohlfarth, Steven Ray Wilson, Ase Marit Leere Oiestad
Summary: This study aimed to discover and elucidate the major metabolites of three novel fentanyl analogues, providing important markers for confirming the intake of these compounds in forensic cases.
FORENSIC TOXICOLOGY
(2021)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Steven Ray Wilson, Elsa Lundanes
Summary: This review focuses on the application of nLC-MS in sample preparation, particularly through online upfront devices and online reaction systems to improve the sensitivity and robustness of samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Malgorzata Zawadzka, Beatrix Racz, Dario Ambrosini, Carl Henrik Goerbitz, Jens Preben Morth, Alistair L. Wilkins, Anja Osteby, Katja Benedikte Presto Elgstoen, Elsa Lundanes, Frode Rise, Amund Ringvold, Steven Ray Wilson
Summary: Research discovered an unknown UV-absorbing compound in the aqueous humor of geese, yet the protective compound X was not found. It is possible that the level of UV protective compound in goose aqueous humor is lower than previously identified compounds, or that it may have degraded during the isolation and chromatographic purification processes used.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Froydis Sved Skottvoll, Frederik Andre Hansen, Sean Harrison, Ida Sneis Boger, Ago Mrsa, Magnus Saed Restan, Matthias Stein, Elsa Lundanes, Stig Pedersen-Bjergaard, Aleksandra Aizenshtadt, Stefan Krauss, Gareth Sullivan, Inger Lise Bogen, Steven Ray Wilson
Summary: Liver organoids can be effectively analyzed using electromembrane extraction (EME) for precise drug development and toxicity screening. Multiwell EME technology allows for simple and repeatable monitoring of drug metabolism kinetics, and is compatible with chromatographic separation methods. Liver organoids and EME are well-suited for mass spectrometry (MS)-based measurements.
ANALYTICAL CHEMISTRY
(2021)
Article
Hematology
Yun Zhang, Lichong Shen, Katja Dreissigacker, Honglin Zhu, Thuong Trinh-Minh, Xianyi Meng, Cuong Tran-Manh, Clara Dees, Alexandru-Emil Matei, Chih-Wei Chen, Markus Ditschkowski, Stefan Krauss, Julia Winkler, Daniel Wolff, Mirjana Ziemer, Andreas Beilhack, Sigrid Karrer, Wolfgang Herr, Andreas Mackensen, Georg Schett, Bernd M. Spriewald, Joerg H. W. Distler
Summary: The inhibition of canonical WNT signaling plays an important role in the prevention and treatment of sclerodermatous cGVHD, with little impact on the graft-versus-leukemia effect. This treatment effectively prevents the development of clinical and histological features in sclGVHD models, suggesting potential translational applications due to the availability of clinically-used pyrvinium and ongoing clinical trials for tankyrase inhibitors.
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Elsa Lundanes, Tuula A. Nyman, Frode S. Berven, Steven Ray Wilson
Summary: Single-cell analysis provides in-depth insights into diseases and diagnostics, with liquid chromatography playing a crucial role. The development of novel sample preparation techniques and improvements in mass spectrometry are enhancing the promise of single-cell analysis. However, technical challenges and the need for higher throughput and robustness may lead to the reinvention of alternative nano LC column formats.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Hanne Bendiksen Skogvold, Elise Mork Sandas, Anja Osteby, Camilla Lokken, Helge Rootwelt, Per Ola Ronning, Steven Ray Wilson, Katja Benedikte Presto Elgstoen
Summary: A single LC-MS method was developed for DBS metabolite analysis in clinical applications such as newborn screening, allowing for simultaneous analysis of a wide range of metabolites. The method utilized a diphenyl column, a multi-linear solvent gradient, and tailored MS settings to enhance sensitivity and reproducibility for diverse metabolites. The performance of the method was suitable for both untargeted and targeted approaches in clinically relevant experiments.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Multidisciplinary Sciences
Line Mygland, Shoshy Alam Brinch, Martin Frank Strand, Petter Angell Olsen, Aleksandra Aizenshtadt, Kaja Lund, Nina Therese Solberg, Max Lycke, Tor Espen Thorvaldsen, Sandra Espada, Dorna Misaghian, Christian M. Page, Oleg Agafonov, Stale Nygard, Nai-Wen Chi, Eva Lin, Jenille Tan, Yihong Yu, Mike Costa, Stefan Krauss, Jo Waaler
Summary: Small-molecule tankyrase 1 and tankyrase 2 (TNKS1/2) inhibitors have shown to be effective antitumor agents in specific tumor cell lines, with the ability to inhibit key signaling pathways and reduce expression of crucial oncogenes, ultimately impairing cell growth. Additionally, these inhibitors induce the accumulation of beta-catenin degradasomes containing TNKS1/2, which interact with YAP and angiomotin proteins, leading to attenuation of YAP signaling. Further research is needed to fully evaluate the potential of TNKS inhibitors in cancer treatment.
Article
Chemistry, Analytical
Christine Olsen, Elisa Wiborg, Elsa Lundanes, Shadab Abadpour, Hanne Scholz, Steven Ray Wilson
Summary: The research focuses on investigating tools for coupling pancreas-on-a-chip systems with mass spectrometry. By using an inter-chain cleavage reactor and nano liquid chromatography-mass spectrometry system, the researchers achieved on-line cleavage of insulin's disulfide bonds and obtained robust fragmentation mass spectra. The use of a silica monolith in the nano liquid chromatography format improved chromatographic performance compared to particle-packed columns.
SEPARATION SCIENCE PLUS
(2022)
Article
Chemistry, Analytical
Stian Kogler, Aleksandra Aizenshtadt, Sean Harrison, Froydis Sved Skottvoll, Henriette Engen Berg, Shadab Abadpour, Hanne Scholz, Gareth Sullivan, Bernd Thiede, Elsa Lundanes, Inger Lise Bogen, Stefan Krauss, Hanne Roberg-Larsen, Steven Ray Wilson
Summary: Organoids, laboratory-grown organ models developed from stem cells, have been successfully coupled with liquid chromatography-mass spectrometry (LC-MS) to enable continuous tracking of drug metabolism.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hanne Bendiksen Skogvold, Helge Rootwelt, Leon Reubsaet, Katja Benedikte Presto Elgstoen, Steven Ray Wilson
Summary: Dried blood spot samples offer a simple and safe option for diagnostics, with liquid chromatography-mass spectrometry being a versatile measurement tool. These samples provide valuable information for metabolomics, xenobiotic analysis, and proteomics. Targeted analysis of small molecules is the main application, but there are emerging applications in untargeted metabolomics and proteomics. Applications range from newborn screening to disease diagnostics and monitoring, as well as research into the effects of diet, exercise, xenobiotics, and doping. A variety of dried blood spot products and liquid chromatography-mass spectrometry methods are available, including novel approaches like on-paper sample preparation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Chemistry, Analytical
Stian Kogler, Kristina Saeterdal Komurcu, Christine Olsen, Jun-ya Shoji, Froydis Sved Skottvoll, Stefan Krauss, Steven Ray Wilson, Hanne Roberg-Larsen
Summary: Organoids and organ-on-a-chip devices are important tools for studying various aspects of organs. This review focuses on the application of mass spectrometry in studying organoids and organ-on-a-chip-derived material. It discusses the use of liquid chromatography-mass spectrometry (LC-MS) for proteomics, metabolomics/lipidomics, and hormones, as well as mass spectrometry imaging (MSI) of organoids. The analysis has provided insights into the development and disease of organs, potentially replacing or complementing animal models.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Michal Gregus, Alexander R. Ivanov, Steven Ray Wilson
Summary: Ultralow flow LC utilizes ultra-narrow bore columns and flow rates ranging from pL/min to low nL/min. It provides high sensitivity and resolution, making it suitable for analyzing scarce biological samples. Recent advancements in dedicated instrumentation, technical approaches, and higher throughput have facilitated the application of ultralow flow LC to the analysis of amount-limited samples. This review discusses the types of ultralow flow LC technology, highlights representative applications in bioanalysis, and explores future developments.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Engineering, Biomedical
Jun-ya Shoji, Richard P. Davis, Christine L. Mummery, Stefan Krauss
Summary: Organoids and cells in organ-on-chip platforms replicate higher-level anatomical, physiological, or pathological states of tissues and organs. A meta-analysis based on a quality-controlled text-mining algorithm is performed to map advances in this emerging field. The analysis covers academic publications in the literature and preprint databases published after 2010, and provides a robust framework for researchers to interrogate with their own questions.
ADVANCED HEALTHCARE MATERIALS
(2023)
