Article
Chemistry, Applied
Chang Liu, Yahui Guo, Yuliang Cheng, He Qian
Summary: This study demonstrates that torularhodin can improve gut health by slowly releasing in the colon, increasing the diversity and species abundance of gut microbiota, and regulating metabolic pathways, thus maintaining the balance of the host-gut ecology.
FOOD HYDROCOLLOIDS
(2023)
Article
Biotechnology & Applied Microbiology
Zhi-tao Li, Yu-ying Wang, Hang-yan Ji, Yun Jiang, Min-jie Gao, Xiao-bei Zhan, Zhengyu Jin
Summary: Research on the influence of food nutrition and medicine on the human gut microbiota is of great interest. This study introduces a new in-vitro simulation colon reactor (SCR) as an important tool for evaluating food function. The SCR proves to be effective in breaking up semisolids, simulating gut peristaltic patterns, and promoting the growth of gut microbiota.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Microbiology
Leli Wang, Yiru Zhang, Juan Xu, Chuni Wang, Lanmei Yin, Qiang Tu, Huansheng Yang, Jia Yin
Summary: The abuse of antibiotics in veterinary field is a serious health challenge, leading to environmental selection pressure on bacteria and the rapid spread of antibiotic resistance genes. However, the discovery and application of cost-effective alternatives to antibiotics in pig production is slow. This study identified diverse secondary metabolite genes in porcine intestines, and found variations in microbial biosynthetic gene clusters (BGCs) composition between the ileum and the colon. It also highlighted the importance of exploring the diversity and composition differences of BGCs in gut microbiota for promoting healthy swine production.
Article
Chemistry, Medicinal
Yang Liu, Siyuan Xu, Qiudie Cai, Dawei Li, Hongye Li, Weidong Yang
Summary: In this study, we investigated the interaction between okadaic acid (OA) and gut microbiota using an in vitro fermentation experiment. We found that OA can reshape the bacterial composition and lead to the production of various metabolites. Our findings confirmed the direct interaction between OA and gut bacteria, providing valuable evidence for understanding the metabolic process and complex toxicity of OA.
Review
Veterinary Sciences
Sriniwas Pandey, Eun Sol Kim, Jin Ho Cho, Minho Song, Hyunok Doo, Sheena Kim, Gi Beom Keum, Jinok Kwak, Sumin Ryu, Yejin Choi, Juyoun Kang, Jeong Jae Lee, Hyeun Bum Kim
Summary: Non-digestible carbohydrates in pig's diet, including non-starch polysaccharides, resistant starch, and non-digestible oligosaccharides, play a significant role in swine gut microbial ecology and overall health. Although rich in energy, swine lack the enzymes necessary for their degradation and rely on gut microbes for energy production. However, limited research has been conducted on the bacteria involved in this process, highlighting the importance of investigating and understanding their role in non-digestible carbohydrate breakdown for pig nutrition and health.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Food Science & Technology
Yuxiao Liao, Zhao Peng, Shiyin Xu, Zitong Meng, Dan Li, Xiaolei Zhou, Rui Zhang, Shaojun Shi, Liping Hao, Liegang Liu, Wei Yang
Summary: This study investigates whether deoxynivalenol (DON) can induce intestinal damage through gut microbiota in mice. The results show that DON exposure causes colon tissue damage, disruption of tight junction protein expression, increased oxidative stress and apoptosis in mice. DON exposure also alters the composition and diversity of the gut microbiota as well as the contents of fecal metabolites. Only a decrease in specific gene expression levels in the colon is observed after fecal microbiota transplantation. Additionally, depleting the gut microbiota in mice also results in histological damage and disrupted tight junction protein expression in the colon after DON exposure.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Chu Wan, Kaizhang Wu, Xingyu Lu, Fang Fang, Yaqian Li, Yumin Zhao, Shubo Li, Jie Gao
Summary: The study found that different culture media have varying effects on the bacterial community structure and metabolites, with the AMB medium being the most effective in maintaining a stable bacterial community structure and producing fewer metabolites and short-chain fatty acids. Culturing with AMB medium for 48 hours is considered the most suitable in vitro model for human gut microbiota fermentation, providing an alternative approach for diet and health research.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Nidhi Sori, Nafee Chundanga Poyil, Mahejibin Khan
Summary: This study investigated the effects of Kodo millet (KOM) and Kutki millet (KUM) on gut microbiota and metabolites in human fecal samples. The results showed that different millet groups had varying effects on gut microbiota and metabolites, with different abundances of specific metabolites. The study also identified correlations between beneficial metabolites and specific bacterial genera, and confirmed the upregulation of various metabolic pathways in millet groups.
Article
Microbiology
Austin Campbell, Kristi Gdanetz, Alexander W. W. Schmidt, Thomas M. M. Schmidt
Summary: Hydrogen gas (H-2) produced during carbohydrate fermentation in the human gut microbiome can modulate fermentation and may differentiate individual microbiomes and metabolites. High concentrations of H-2 stimulate the production of anti-inflammatory butyrate, while gut methanogenesis decreases butyrate production and impacts the competitive fitness of butyrate producers.
Article
Food Science & Technology
Ville M. Koistinen, Maria Hedberg, Lin Shi, Anders Johansson, Otto Savolainen, Marko Lehtonen, Anna-Marja Aura, Kati Hanhineva, Rikard Landberg
Summary: This study used metabolomics to identify seven microbial metabolites commonly produced during fermentation and found that a mixture of these metabolites exhibited stronger inhibition against bacteria compared to individual compounds. The results suggest that metabolites produced by LAB may modulate local microbial ecology through synergistic effects.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Chemistry, Applied
Haidi Xu, Bradley L. Reuhs, Thaisa M. Cantu-Jungles, Yunus E. Tuncil, Amandeep Kaur, Anton Terekhov, Eric C. Martens, Bruce R. Hamaker
Summary: This study investigated the relationship between fine structure and fermentability of Corn arabinoxylan (CAX) by the human gut microbiota. The analysis revealed an organized structural feature of CAX, with complex branching patterns. Contrary to conventional views, smaller fiber structures with higher branch complexity fermented slower.
CARBOHYDRATE POLYMERS
(2022)
Article
Food Science & Technology
Ying Yang, Wenyang Tao, Wanyi Zhou, Jingrui Li, Jianrong Xing, Mengfan Luo, Quanqing Tan
Summary: This study used an in vitro fermentation system to investigate the effects of long-term/short-term DOLP treatment on the gut microbiota. The results suggest that continuous consumption for 5 days is the most effective duration for adjusting the gut microbiota.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biochemical Research Methods
Ying Wang, Qin Jiang, Yilin Geng, Yuren Hu, Yue Tang, Jixiang Li, Junmei Zhang, Wolfgang Mayer, Shanmei Liu, Hong-Yu Zhang, Xianghua Yan, Zaiwen Feng
Summary: Gut microbiota plays a crucial role in pig development and health, and is associated with differences in feed efficiency. A Swine Gut Microbiota Federated Query Platform (SGMFQP) is presented, which provides convenient and efficient query service about swine feeding and gut microbiota. The system is based on a Swine Gut Microbiota Ontology (SGMO) and utilizes a template-based query interface, a federated query engine, and a workflow orchestration mechanism to retrieve information from multiple data sources.
Article
Chemistry, Applied
Chuan Zhang, Leilei Yu, Qixiao Zhai, Ruohan Zhao, Jianxin Zhao, Hao Zhang, Wei Chen, Fengwei Tian
Summary: This study utilized an in vitro fermentation system combined with microbiome and metabolome technologies to investigate the interaction between heparin and the gut microbiota. The findings indicate that heparin can be utilized by the gut microbiota, resulting in the production of significant amounts of short chain fatty acids and a decrease in pH. Furthermore, the addition of heparin influenced the relative abundance of specific bacterial species in the gut.
Article
Biochemistry & Molecular Biology
Xincheng Wu, Xiaojun Huang, Wanning Ma, Mingzhi Li, Jiajia Wen, Chunhua Chen, Liandi Liu, Shaoping Nie
Summary: This study compared the intestinal immunomodulatory mechanisms of nine representative bioactive polysaccharides. The results revealed that these polysaccharides promote intestinal immunity through different ways, such as alleviating immune suppression in intestinal mucosal T cells, improving the intestinal microenvironment, promoting the colonization of beneficial bacteria, inhibiting the colonization of harmful bacteria, and alleviating the accumulation of certain metabolites in the large intestine. These findings provide a guideline for the development of superior intestinal immunomodulatory polysaccharides.
Review
Food Science & Technology
Palni Kundra, Carole Rachmuhl, Christophe Lacroix, Annelies Geirnaert
Summary: In patients with inflammatory bowel diseases (IBD), low dietary micronutrient intake and deficiencies are common, which may also affect the functioning of the gut microbiota. There is a need for further research to understand the mechanisms of micronutrient-microbiome-host interactions and their role in alleviating inflammation in IBD.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Microbiology
Alejandro Ramirez Garcia, Jianbo Zhang, Anna Greppi, Florentin Constancias, Esther Wortmann, Muriel Wandres, Katherine Hurley, Alberto Pascual-Garcia, Hans-Joachim Ruscheweyh, Shana J. Sturla, Christophe Lacroix, Clarissa Schwab
Summary: The study found that GDH activity is a common trait of intestinal microbiota determined by a few key taxa, with Anaerobutyricum hallii identified as a key species in GDH metabolism. Supplementation of Anaerobutyricum hallii can enhance GDH activity and acrolein release, facilitating the transformation of HCA and reducing fermentation activity.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Nutrition & Dietetics
Josep Bassaganya-Riera, Elliot M. Berry, Ellen E. Blaak, Barbara Burlingame, Johannes le Coutre, Willem van Eden, Ahmed El-Sohemy, J. Bruce German, Dietrich Knorr, Christophe Lacroix, Maurizio Muscaritoli, David C. Nieman, Michael Rychlik, Andrew Scholey, Mauro Serafini
Summary: In the past five years, significant progress has been made in nutrition science, such as the integration of Sustainable Development Goals and nutritional science, as well as research on the Human Microbiome. Looking ahead to the next five years, emphasis on health and sustainability will be the main focus of nutrition science.
FRONTIERS IN NUTRITION
(2021)
Article
Microbiology
Nize Otaru, Kun Ye, Denisa Mujezinovic, Laura Berchtold, Florentin Constancias, Fabian A. Cornejo, Adam Krzystek, Tomas de Wouters, Christian Braegger, Christophe Lacroix, Benoit Pugin
Summary: Research investigated GABA production of Bacteroides and its protective role in acid stress, revealing the widespread presence of the GAD system in Bacteroides and its potential as the only amino acid-dependent acid tolerance system in the genus.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Elora Fournier, Charlene Roussel, Alessandra Dominicis, Delphine Ley, Marie-Agnes Peyron, Valerie Collado, Muriel Mercier-Bonin, Christophe Lacroix, Monique Alric, Tom Van de Wiele, Christophe Chassard, Lucie Etienne-Mesmin, Stephanie Blanquet-Diot
Summary: This article reviews the development of child gut physiology and the use of in vitro models to study it. Research has shown that the child digestive tract undergoes significant changes in the first few years of life, including colonization of gut microbiota and exposure to environmental compounds. In vitro models are increasingly used due to ethical and technical limitations, and can simulate different stages of digestion from the oral phase to the colon compartment. These models have important applications in nutritional, pharmaceutical, and microbiological studies.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Nutrition & Dietetics
Teresa Demuth, Veronica Edwards, Lea Bircher, Christophe Lacroix, Laura Nystrom, Annelies Geirnaert
Summary: The study found that the structural alterations of soluble arabinoxylan (AX) from wheat bran and rye flour can affect in vitro human colon fermentation, with wheat bran AX fermenting faster than rye flour AX. Increased levels of bound phenolic acids resulting from processing were identified as inhibiting factors for AX fermentation kinetics.
FRONTIERS IN NUTRITION
(2021)
Article
Microbiology
Benoit Pugin, Serafina Pluss, Denisa Mujezinovic, Rikke C. Nielsen, Christophe Lacroix
Summary: The study evaluated UV-spectrophotometric and H2O2-based fluorescent assays to identify uricase activity in different bacterial species. The results showed that the UV-spectrophotometric method using whole bacterial suspension is a feasible and high-throughput approach for screening uricase activity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Julia Isenring, Marc J. A. Stevens, Christoph Jans, Christophe Lacroix, Annelies Geirnaert
Summary: This study investigates the colonization process of Lactiplantibacillus plantarum in the in vitro colonic community, showing that colonization levels are dependent on the microbiota features rather than the introduction parameters. The study also demonstrates that the carrying capacity decreases over time and is correlated with richness and evenness. The findings provide important insights into colonizer-microbiota interactions and probiotic strategies.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Sabrine Naimi, Severine Zirah, Anna Greppi, Christophe Lacroix, Sylvie Rebuffat, Ismail Fliss
Summary: This study evaluated the impact of MccJ25 on the composition and metabolic activity of the swine colonic microbiota in vitro. The results showed that MccJ25 only caused subtle changes in microbial diversity and metabolome, while rifampicin induced significant modification in amino acid levels. Although further validation is needed in vivo, this study provides a first proof of concept for considering MccJ25 as an alternative to antibiotics for veterinary and farming applications, considering its pathogen-selective and potent inhibitory activity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Van T. Pham, Anna Greppi, Christophe Chassard, Christian Braegger, Christophe Lacroix
Summary: This study provides important insights into the colonization of functional groups involved in lactate metabolism and butyrate production in the first two years of life. The research revealed dynamic changes in the gut microbiota of infants, with a switch from Veillonella producing propionate in the first year to Anaerobutyrycum hallii producing butyrate in the second year. Additionally, the weaning period between 6 and 10 months was identified as a significant time for an increase in butyrate producers and fecal butyrate concentration. Correlation analyses also suggested the metabolic cross-feeding of hydrogen in infants, which is a new finding in this study.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Medicinal
Alejandro Ramirez Garcia, Katherine Hurley, Giovanni Marastoni, Mederic Diard, Sophie Hofer, Anna Greppi, Wolf-Dietrich Hardt, Christophe Lacroix, Shana J. Sturla, Clarissa Schwab
Summary: GDH-competent microbes have the ability to produce acrolein from glycerol, which can result in the formation of DNA adducts. However, high concentrations of glycerol are required for this process.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Nutrition & Dietetics
Vanesa Natalin Rocha Martin, Christophe Del'Homme, Christophe Chassard, Clarissa Schwab, Christian Braegger, Annick Bernalier-Donadille, Christophe Lacroix
Summary: In recent years, the relationship between gut microbiota and host health has been a major focus of research. Using animal models inoculated with infant fecal samples, researchers have found that an imbalance in gut microbiota and abnormal lactate metabolism appear to be the main causes of infant colic. They also identified a correlation between excessive H-2 production and certain bacteria in the infant gut, suggesting that an overproduction of H-2 may contribute to colic. Additionally, supplementation of specific bacteria, such as Cutibacterium avidum P279, was found to reduce colic symptoms.
FRONTIERS IN NUTRITION
(2022)
Article
Nutrition & Dietetics
Palni Kundra, Annelies Geirnaert, Benoit Pugin, Paola Morales Martinez, Christophe Lacroix, Anna Greppi
Summary: This study investigated the B12 production of human fecal microbiota and the effects of different levels of B12 on composition and activity. The results showed that healthy human gut microbial communities have the capacity to produce B12 independently of dietary intake, and supplementation of exogenous B12 may have limited impact on the gut microbial community composition and function.
FRONTIERS IN NUTRITION
(2022)
Article
Gastroenterology & Hepatology
Fabienne Kurt, Gabriel E. Leventhal, Marianne Rebecca Spalinger, Laura Anthamatten, Philipp Rogalla von Bieberstein, Carmen Menzi, Markus Reichlin, Marco Meola, Florian Rosenthal, Gerhard Rogler, Christophe Lacroix, Tomas de Wouters
Summary: By selecting nine strains and continuous co-culturing, a stable microbial consortium that emulates the central metabolic pathways of carbohydrate fermentation in the healthy human gut microbiota was constructed. The function-based consortium showed an efficacy comparable to FMT in counteracting acute colitis, while an equivalent mix of strains failed to match FMT. The approach of combining a bottom-up functional design with continuous co-cultivation is proposed as a powerful strategy to produce robust functionally designed synthetic consortia for therapeutic use.
Article
Biotechnology & Applied Microbiology
Lea Bircher, Alain M. Sourabie, Marijana Paurevic, Janina Hochuli, Annelies Geirnaert, Chloe Navas, Benoit Drogue, Christophe Lacroix
Summary: In this study, we evaluated different yeast-based nutrients and B-vitamins for the cultivation of Faecalibacterium duncaniae A2-165. The results showed that yeast extracts promoted the growth of this microorganism, while yeast peptones and yeast cell wall product lacked essential growth factors. Additionally, B5 vitamin was identified as a strong growth promoter. Selecting nutritionally suitable yeast-based nutrients and combining them with other key nutrients can optimize the production of this microorganism with high yields.
MICROBIAL BIOTECHNOLOGY
(2023)
