Article
Engineering, Biomedical
Abderrahman Tamoud, Fahmi Zairi, Amar Mesbah, Fahed Zairi
Summary: This study developed a fully three-dimensional human annulus fibrosus model to predict the regional anisotropic multiaxial damage of the intervertebral disc. By identifying model parameters using experimental data and considering effective interactions between adjacent layers and chemical-induced volumetric strain, a multi-layered annulus model was constructed. The model predictions were analyzed under various loading modes and compared to experimental data for validation, showing potential for predicting deformation-induced damage and failure under multiaxial loading histories.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Joana Caldeira, Adam Celiz, Nicolas Newell
Summary: This study proposed a biomechanical testing method to evaluate the ability of bioadhesives to seal annulus fibrosus tears. The results showed that the tough hydrogel adhesive could effectively restore the burst pressure of intervertebral discs, providing a new approach for the treatment of disc degeneration.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Engineering, Biomedical
Dalin Wang, Alon Lai, Jennifer Gansau, Philip Nasser, Yunsoo Lee, Damien M. Laudier, James C. Iatridis
Summary: This study established an ex-vivo rat lumbar endplate injury model and characterized the effects of endplate injury on motion segment biomechanical properties. Compared to annulus fibrosus injury, endplate injury had the greatest impact on axial biomechanical properties, while annulus fibrosus injury primarily affected torsional properties.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Cell & Tissue Engineering
Ana P. Peredo, Sarah E. Gullbrand, Harvey E. Smith, Robert L. Mauck
Summary: Intervertebral disc herniation can cause neural compression, pain, and loss of function, with surgical intervention carrying risks of reherniation and further degeneration. Traditional repair methods fail to address rupture of the annulus fibrosus, highlighting the need to mobilize cellular players for improved healing outcomes.
TISSUE ENGINEERING PART B-REVIEWS
(2021)
Article
Engineering, Biomedical
Chen Liu, Yu Li, Yu Zhang, Hongguang Xu
Summary: The study synthesized DAFM/PECUU-blended fibrous scaffolds using a coaxial electrospinning method to culture AFSCs, and found that with increased elasticity of the scaffold material, collagen type I gene expression increased, while collagen type II and aggrecan gene expression showed the opposite trend.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Engineering, Biomedical
Han Wang, Di Wang, Beier Luo, Dong Wang, Haoruo Jia, Pandi Peng, Qiliang Shang, Jianxin Mao, Chu Gao, Ye Peng, Lu Gan, Junjie Du, Zhuojing Luo, Liu Yang
Summary: This study decoded the mouse AF cell atlas, identified a new population of AF cells, and proposed a novel strategy for AF reconstruction based on composite hydrogels, demonstrating the potential for successful reconstruction of AF defects.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Ketao Wang, Ji Li, Yuxing Wang, Yaqiang Wang, Yuanyuan Qin, Fei Yang, Mingzhu Zhang, Heng Zhu, Zhongli Li
Summary: The study demonstrated the efficacy of a triple-optimized PRP + CPC construct for cartilage regeneration by enhancing biomechanical properties and suitable biochemical signals. Both in vitro and in vivo experiments showed that the construct could generate tissue resembling cartilage, with better biomechanical recovery compared to other constructs.
BIOACTIVE MATERIALS
(2021)
Review
Orthopedics
Ana P. Peredo, Sarah E. Gullbrand, Robert L. Mauck, Harvey E. Smith
Summary: Intervertebral disc (IVD) herniations, caused by annulus fibrosus (AF) tears, are common among adults in the third to fifth decade of life. Symptomatic herniations can lead to back pain, numbness, and tingling, resulting in extreme physical disability. Despite advancements in surgical techniques, challenges remain in effectively restoring the native tissue structure and function of the AF.
Article
Engineering, Biomedical
Yi Zheng, Borui Xue, Bin Wei, Bing Xia, Shengyou Li, Xue Gao, Yiming Hao, Yitao Wei, Lingli Guo, Haining Wu, Yujie Yang, Xueli Gao, Beibei Yu, Yongfeng Zhang, Shijie Yang, Zhuojing Luo, Teng Ma, Jinghui Huang
Summary: The repair of annulus fibrosus (AF) defect after discectomy in the intervertebral disc (IVD) has been a challenge due to hostile microenvironments, particularly compression and hypoxia. This study developed a oxygen-releasing scaffold using perfluorotributylamine (PFTBA) core-shell fibers to promote endogenous repair in the AF. The oxygen released from the PFTBA fibers protected annulus fibrosus stem cells (AFSCs) from hypoxia-induced apoptosis and promoted proliferation, migration, and extracellular matrix (ECM) production in AFSCs. In vivo studies showed that the oxygen-supplying fibers improved disc degeneration after discectomy by enhancing disc height and morphological integrity.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Shruthi Karnam, Nikolai P. Skiba, Ponugoti Rao
Summary: This study characterized lenses derived from dystrophin-deficient mice and found changes in the distribution of Dp71 and other integral membrane proteins, resulting in decreased lens stiffness. The reductions in DAPC levels in dystrophin-deficient lenses indicate the importance of dystrophin in maintaining lens clarity and function.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Materials Science, Multidisciplinary
Zetao Wang, Huitong Luo, Zhiyu Zhou, Zhongyuan He, Shuangli Zhu, Dingguo Li, Huichang Gao, Xiaodong Cao
Summary: A multifunctional silk fibroin cryogel scaffold loaded with annulus fibrosus cell-derived exosomes was constructed to inhibit intervertebral disc degeneration and promote regeneration of defects by regulating the immune microenvironment and scavenging ROS. It shows potential application for AF defect repair.
APPLIED MATERIALS TODAY
(2022)
Article
Biotechnology & Applied Microbiology
Rongrong Deng, Ran Kang, Xiaoyu Jin, Zihan Wang, Xin Liu, Qing Wang, Lin Xie
Summary: In this study, it was found that the sticky Fib-T-G biological gel could effectively repair AF fissures in rats and increase the expression of AF-related proteins and mechanotransduction-related proteins. Furthermore, mechanical strain was shown to promote the differentiation of AF-hMSCs, and this effect could be regulated by inhibiting or overexpressing the RhoA/ROCK1 pathway.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Minhao Zhou, Erin S. Archibeck, Yarah Feteih, Yousuf Abubakr, Grace D. O'Connell
Summary: Growing clinical evidence suggests a correlation between diabetes and more frequent and severe intervertebral disc failure. This study used a combined experimental-computational approach to evaluate the effect of advanced glycation end-products (AGEs) on annulus fibrosus (AF) tensile mechanics. The results showed that AGEs increase AF bulk tensile mechanical properties at the risk of tissue mechanical failure or catabolic remodeling. Computational results indicate that crosslinks along the fiber direction account for the increased tissue stiffness with glycation.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Qifan Yu, Feng Han, Zhangqin Yuan, Zhuang Zhu, Changjiang Liu, Zhengdong Tu, Qianping Guo, Runze Zhao, Weidong Zhang, Huan Wang, Haijiao Mao, Bin Li, Caihong Zhu
Summary: In this study, researchers developed a nanofibrous scaffold loaded with fucoidan, a natural marine polysaccharide with anti-inflammatory and antioxidative functions, to alleviate the adverse microenvironment of intervertebral disc degeneration (IDD). The results showed that the fucoidan-loaded scaffold significantly decreased the expression of genes and proteins related to inflammation and oxidative stress. Moreover, it reduced the degradation of extracellular matrix and promoted disc repair, indicating promising potential for IDD treatment.
ACTA BIOMATERIALIA
(2022)
Article
Biotechnology & Applied Microbiology
K. Josh Briar, John G. McMorran, Diane E. Gregory
Summary: The separation or delamination between layers of the intervertebral disc can contribute to degeneration development, and the rate of separation affects the mechanical response of the interlamellar matrix. The study found that the adhesion strength between layers varies with different separation rates, and increased rates of separation may lead to non-uniform strength, which can potentially cause microtrauma and delamination.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Hanghang Fang, Yongjie Sha, Liang Yang, Jingjing Jiang, Lichen Yin, Jiaying Li, Bin Li, Bert Klumperman, Zhiyuan Zhong, Fenghua Meng
Summary: By nano-formulating hydroxychloroquine and designing it as a macrophage-targeted nanotherapeutic, it is possible to achieve highly effective treatment for rheumatoid arthritis. This therapeutic approach can induce repolarization of macrophages towards an anti-inflammatory phenotype, effectively reducing inflammation, and has shown significant therapeutic effects in mouse models.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Jiayuan Wang, Huan Wang, Yong Wang, Zhao Liu, Zexi Li, Jiaying Li, Qixin Chen, Qingchen Meng, Wenmiao Will Shu, Junxi Wu, Can Xiao, Fengxuan Han, Bin Li
Summary: Vascularization is a critical factor in bone tissue engineering, and lack of endothelialization remains a challenge. This study developed a technique to fabricate endothelialized biomimetic microvessels using microfluidic technology, which showed promising results in promoting osteogenesis and bone regeneration both in vitro and in vivo. These findings suggest that endothelialized BMVs could be an effective strategy in bone tissue engineering.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Multidisciplinary
Jiaying Li, Fengxuan Han, Jinjin Ma, Huan Wang, Jun Pan, Guangbao Yang, He Zhao, Jiayue Zhao, Jinbo Liu, Zhuang Liu, Bin Li
Summary: This study visualizes the spatial and temporal profile of ROS in the bone injury microenvironment using photoacoustic imaging technique for the first time. It demonstrates that the levels of H2O2 increase significantly upon bone injury, peak at a certain stage of bone healing, and gradually decrease towards baseline level. Regulating ROS with hollow manganese dioxide nanoparticles (hMNPs) can promote cell growth and repair in bone injury environments.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Biomedical
Tao Gui, Lijun Luo, Bonirath Chhay, Leilei Zhong, Yulong Wei, Lutian Yao, Wei Yu, Jun Li, Charles L. Nelson, Andrew Tsourkas, Ling Qin, Zhiliang Cheng
Summary: Oxidative stress and reactive oxygen species play important roles in the development and progression of osteoarthritis (OA). This study utilized SOD-loaded nanoparticles to deliver antioxidant enzymes to mouse knee joints, demonstrating the accumulation of nanoparticles in the synovium and reduction of oxidative damage. Intra-articular injections of the nanoparticles were effective in attenuating OA initiation and progression, via reduction of reactive oxygen species production and catabolic protease synthesis.
Article
Anatomy & Morphology
Deva D. Chan, Maleeha Mashiatulla, Jun Li, Ryan D. Ross, Meghana Pendyala, Amit Patwa, Mark W. Grinstaff, Anna Plaas, D. Rick Sumner
Summary: This study uses contrast-enhanced micro-computed tomography to track the spatiotemporal patterns of cartilage and bone degeneration in a mouse model of osteoarthritis. The findings provide important insights for the development of targeted therapies for osteoarthritis.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2023)
Article
Engineering, Biomedical
Zhao Liu, Huan Wang, Zhangqin Yuan, Qiang Wei, Fengxuan Han, Song Chen, Hao Xu, Jiaying Li, Jiayuan Wang, Zexi Li, Qixin Chen, Jerry Fuh, Lin Ding, Hui Wang, Bin Li
Summary: In this study, high-resolution polycaprolactone (PCL) scaffolds were successfully fabricated using a new electrohydrodynamic 3D printing technique, allowing for accurate simulation of the native annulus fibrosus (AF) structure. In vivo experiments showed that the tissue-engineered intervertebral discs (TE-IVDs) constructed with the 3D printed AF scaffold maintained disc height, reduced loss of nucleus pulposus (NP) water content, and partially restored the biomechanical function of the intervertebral disc.
Article
Engineering, Biomedical
Jinjin Ma, Jiaying Li, Xingran Wang, Meimei Li, Wenwen Teng, Zihan Tao, Jile Xie, Yanxia Ma, Qin Shi, Bin Li, Saijilafu
Summary: In this study, GDNF-loaded PDA nanoparticle-based anisotropic scaffolds were developed for spinal cord repair. The mesoporous PDA nanoparticles efficiently scavenged ROS and promoted microglia M2 polarization, inhibiting inflammatory response and providing a favorable microenvironment for nerve cell survival. Furthermore, the GDNF encapsulated in the nanoparticles promoted axon regeneration and locomotor functional recovery.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Orthopedics
Heng Sun, Huan Wang, Weidong Zhang, Haijiao Mao, Bin Li
Summary: Two functional cell subpopulations in the annulus fibrosus (AF) were identified, including one with stemness characteristics and another associated with vascularization during intervertebral disc degeneration (IDD). These findings provide potential cell sources and regulation targets for IDD treatment and tissue repair.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2023)
Article
Materials Science, Multidisciplinary
Weidong Zhang, Huan Wang, Genglei Chu, Li Yu, Feng Han, Qifan Yu, Youhua Wang, Bin Li
Summary: In this study, micropatterned collagen scaffolds were fabricated to mimic the microstructural features of annulus fibrosus (AF) and mechanically stimulate bone marrow mesenchymal stromal cells (BMSCs). The aligned cells showed enhanced expression of matrix anabolism-related genes and proteins under mechanical loading. Furthermore, Caveolin-1 (CAV1) was found to mediate the nuclear translocation of Yes-associated protein (YAP) in response to external mechanical cues. The biomimetic collagen membranes formed angle-ply and multilayered tissues that showed improved restoration of the disc in a rat model.
APPLIED MATERIALS TODAY
(2023)
Article
Engineering, Biomedical
Yifeng Xia, Jingnan An, Jiaying Li, Wenxing Gu, Yifan Zhang, Songsong Zhao, Cenzhu Zhao, Yang Xu, Bin Li, Zhiyuan Zhong, Fenghua Meng
Summary: This study found that transferrin-guided polymersomes (TPs) significantly enhance the targetability, potency, and safety of the PLK1 inhibitor volasertib (Vol) against acute myeloid leukemia (AML). Vol-loaded TPs (TPVol) exhibited the highest cellular uptake, effective down-regulation of p-PLK1, p-PTEN, and p-AKT, and superior apoptotic activity compared to free Vol in leukemic cells. TPVol showed 6-fold higher AUC and notable accumulation in AML-residing bone marrow after intravenous injection. In an orthotopic leukemic model, TPVol significantly reduced leukemic cell proportions in peripheral blood, bone marrow, liver, and spleen, enhanced mouse survival rate, and impeded bone loss. This transferrin-guided nano-delivery strategy appears to be an interesting approach for the development of novel treatments for AML.
BIOACTIVE MATERIALS
(2023)
Review
Engineering, Biomedical
Dachuan Liu, Chen Cui, Weicheng Chen, Jiaxu Shi, Bin Li, Song Chen
Summary: This review provides an overview of commonly used biodegradable bone cements, such as calcium phosphates, calcium sulfates, and organic-inorganic composites. The possible degradation mechanism and clinical performance of these cements are summarized. The aim is to inspire and provide references for researchers in this field.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Song Chen, Huan Wang, Dachuan Liu, Jianzhong Bai, Havard Jostein Haugen, Bin Li, Hongji Yan
Summary: The design principle of osteogenic bone grafts has shifted from immunological inertness to limiting foreign body response to combined osteoimmunomodulatory activity. The newly developed immunomodulatory mucin hydrogels have shown low complement activation and suppressed macrophage release and activation after implantation. However, their immunoregulatory activity has not been studied in the context of tissue repair.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Shenghao Wang, Wenbo He, Huan Wang, Dachuan Liu, Miao Wang, Huilin Yang, Guoqing Pan, Bin Li
Summary: In this study, a dynamic hydrogel that mimics the antimicrobial and reparative properties of hematoma was designed for treating infected bone fractures. The hydrogel rapidly eradicated bacteria, improved bone regeneration, and restored the local inflammatory microenvironment. These findings suggest that the use of hematoma-like dynamic hydrogel could revolutionize surgical strategies against susceptible bone fractures.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Wenmin Guo, Huanhuan Xu, Dachuan Liu, Li Dong, Ting Liang, Bin Li, Bin Meng, Song Chen
Summary: The stress distribution of six lattice-inspired structures was investigated using finite element simulations, and scaffolds with pre-designed structures were prepared using selective laser sintering (SLS) technology. Scaffolds with face-centered cubic (FCC) structures exhibited the highest compressive strength. Composite scaffolds composed of polylactic acid/anhydrous calcium hydrogen phosphate (PLA/DCPA) showed good mechanical properties and bioactivity, promoting cell proliferation significantly and showing excellent osteogenic performance. FCC structures are promising for bone tissue engineering.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Biomedical
Qiang Wei, Dachuan Liu, Genglei Chu, Qifan Yu, Zhao Liu, Jiaying Li, Qingchen Meng, Weishan Wang, Fengxuan Han, Bin Li
Summary: This study developed an injectable hydrogel for annulus fibrosus (AF) repair by combining polyethylene glycol diacrylate (PEGDA) and decellularized annulus fibrosus matrix (DAFM). Transforming growth factor-beta 1 (TGF-beta 1) was also incorporated into the hydrogel. The hydrogel showed improved mechanical strength, facilitated AF cell migration, adhesion, proliferation, and increased extracellular matrix (ECM) production. In vivo experiments showed that the hydrogel effectively repaired AF defects and restored biomechanical properties of the disc.
BIOACTIVE MATERIALS
(2023)
