Article
Biochemistry & Molecular Biology
Giovanna Cesaro, Heloisa Tramontin da Soler, Eloise Pavao Guerra-Slompo, Ahmed Haouz, Pierre Legrand, Nilson Ivo Tonin Zanchin, Beatriz Gomes Guimaraes
Summary: Rrp44/Dis3 is a conserved eukaryotic ribonuclease that plays a role in processing and degradation of almost all types of RNA. Depletion of Rrp44 in Trypanosoma brucei blocks maturation of ribosomal RNA, leading to disruption of ribosome synthesis and inhibition of cell proliferation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jinjin Li, Weiwei Wang, Jinhong Yuan, Jinyu Xu, Lifei He, Xinying Zhang, Haiyan Zhang
Summary: Plants have evolved sophisticated mechanisms to adjust to nutrient deficiency or excess. The COPT2 protein in Arabidopsis thaliana is transcriptionally regulated in response to changing levels of cellular copper, but its activity is subject to degradation in response to excess copper. The protein's stability is affected by proteasome inhibition and its ubiquitination status, highlighting the complexity of plasma membrane transporter degradation mechanisms.
Article
Plant Sciences
Kirsty McCready, Victoria Spencer, Francisco Jacome-Blasquez, Jamie Burnett, Itzel Margarita Viveros Sanchez, Zara Riches, Minsung Kim
Summary: The TOR protein kinase is crucial for plantlet formation in Kalanchoe spp., as it regulates nutrient utilization, energy supply, and development pathways. It interacts with auxin pathways to control plantlet root development.
Article
Plant Sciences
Jia Feng, Laichao Cheng, Zhenying Zhu, Feiqi Yu, Cheng Dai, Zhongchi Liu, Wen-Wu Guo, Xiao-Meng Wu, Chunying Kang
Summary: Axillary bud development plays a crucial role in plant architecture, and the genetic control of runner production in strawberry remains largely unknown. This study identified a mutant lam lacking axillary meristems, which was found to be associated with a gene encoding a GRAS transcription factor. Furthermore, functional studies revealed the sequential role of LAM with gibberellic acid in stamen and runner formation, providing insights into the molecular regulation of these structures in strawberry.
Article
Plant Sciences
Lihong Li, Melaku Garsamo, Jing Yuan, Xiaojin Wang, Susan H. Lam, Kranthi Varala, Leonor C. Boavida, Yun Zhou, Xing Liu
Summary: The activity of CAND1 is essential for cells or tissues expressing a high level of F-box proteins, and it plays an important role in maintaining normal cellular functions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Ikuko Maejima, Taichi Hara, Satoshi Tsukamoto, Hiroyuki Koizumi, Takeshi Kawauchi, Tomoko Akuzawa, Rika Hirai, Hisae Kobayashi, Inoya Isobe, Kazuo Emoto, Hidetaka Kosako, Ken Sato
Summary: RAB35 is a multifunctional small GTPase that regulates endocytic recycling, cytoskeletal rearrangement, and cytokinesis. Our findings revealed that RAB35 is required for precise neuronal distribution in the developing hippocampus by regulating the expression of cell adhesion molecules, thereby influencing spatial memory. Mice with a brain-specific knockout of the Rab35 GTPase exhibit defects in spatial memory and hippocampal lamination, outlining a key role for Rab35 in hippocampal development and function.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shangyu Gao, Yu Sun, Xiaoguang Chen, Changhua Zhu, Xiaoye Liu, Wenlei Wang, Lijun Gan, Yanwu Lu, Frank Schaarschmidt, Marco Herde, Claus-Peter Witte, Mingjia Chen
Summary: This study reveals that free 5-methylcytidine (5mC) and 5-methyl-2'-deoxycytidine are produced from nucleic acid turnover in Arabidopsis thaliana. The degradation process of these cytidines, which is unclear in eukaryotes, is elucidated. It is found that 5-methyluridine (5mU) is mainly generated from RNA, and most 5mU is directly released from RNA without a 5mC intermediate. The removal of 5mU is shown to be an important function of pyrimidine degradation in many organisms, protecting RNA from stochastic m(5)U modification in plants.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Plant Sciences
Sofia Shevtsov-Tal, Corinne Best, Roei Matan, Sam A. Chandran, Gregory G. Brown, Oren Ostersetzer-Biran
Summary: Plant organellar introns have degenerated during evolution, relying on host-acting protein cofactors for splicing, which may link cellular signals with respiratory functions.
Article
Plant Sciences
Kosuke Moriwaki, Shuichi Yanagisawa, Koh Iba, Juntaro Negi
Summary: In this study, two crucial cis-acting elements, 5'-CACGAGA-3 'and 5'-CACATGTTTCCC-3', were found to be essential for the guard cell-specific expression of SCAP1 and HT1. The findings reveal a novel transcriptional regulatory mechanism that synchronously promotes the expression of multiple genes required for stomatal maturation and function.
Article
Plant Sciences
Yun-Ji Shin, Ulrike Vavra, Richard Strasser
Summary: The study examined the degradation pathways of misfolded glycoproteins in Arabidopsis. The results suggest that severely misfolded proteins are targeted to ERAD, while less severely misfolded proteins are transported to the vacuole for degradation through the efficient ER export signal provided by the GPI anchor.
Article
Plant Sciences
Wei-Chih Lin, Ya-Huei Chen, Shin-Yuan Gu, Hwei-Ling Shen, Kai-Chau Huang, Wen-Dar Lin, Men-Chi Chang, Ing-Feng Chang, Chwan-Yang Hong, Wan-Hsing Cheng
Summary: Plant chloroplast RNA splicing and ribosome maturation (CRM)-domain-containing proteins are involved in splicing of group I or II introns in chloroplasts, with their functions in mitochondria less clear. The study found that Arabidopsis thaliana CFM6, a protein with a single CRM domain, plays a crucial role in mitochondrial complex I biogenesis and normal plant growth by specifically participating in the splicing of nad5 intron 4. Mutations in CFM6 lead to severe growth defects and splicing failures in nad5 intron 4.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Weihan Fu, Zheng Cui, Jia Guo, Xiayu Cui, Guomao Han, Yunpeng Zhu, Jinju Hu, Xiaoling Gao, Yeqing Li, Min Xu, Aigen Fu, Fei Wang
Summary: Excess light can damage photosystem II (PSII) where electron transfer occurs. This study focuses on high light (HL) responsive immunophilin genes in green algae and identifies a chloroplast lumen-localized immunophilin, CYN28, as essential for HL tolerance. Lack of CYN28 leads to HL hypersensitivity, reduced accumulation of PSII supercomplexes, and compromised PSII repair. The study also reveals the role of CYN28 in regulating the turnover of thylakoid FtsH, a key protein involved in PSII repair, through its peptidyl-prolyl isomerase (PPIase) activity.
Article
Multidisciplinary Sciences
Seyed A. R. Mousavi, Adrienne E. Dubin, Wei-Zheng Zeng, Adam M. Coombs, Khai Do, Darian A. Ghadiri, William T. Keenan, Chennan Ge, Yunde Zhao, Ardem Patapoutian
Summary: PIEZO1 in Arabidopsis thaliana plays an important role in root mechanotransduction, establishing PIEZOs as physiologically relevant mechanosensitive ion channels across animal and plant kingdoms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Carla Brillada, Ooi-Kock Teh, Franck Anicet Ditengou, Chil-Woo Lee, Till Klecker, Bushra Saeed, Giulia Furlan, Marco Zietz, Gerd Hause, Lennart Eschen-Lippold, Wolfgang Hoehenwarter, Justin Lee, Thomas Ott, Marco Trujillo
Summary: Exo70B2 acts as a subunit of the exocyst in the immune response pathway and is involved in regulating transport into vacuoles through interactions with ATG8 and dependence on autophagy. Phosphorylation of Exo70B2 affects its localization and interaction with ATG8, ultimately impacting effector-triggered immunity and sensitivity to BTH. This study provides insights into the molecular mechanisms that divert Exo70B2 from secretion to autophagy for degradation in order to modulate secretory activity during immune responses.
Article
Cell Biology
Ji-Hyun Bae, Jong Hyun Kim
Summary: The control of exosome release plays a significant role in regulating cell survival in TSC-null cells, with mTORC1 inhibition increasing exosome biogenesis and secretion to enhance cell viability under glucose deprivation conditions.
Article
Plant Sciences
Jun Sakaguchi, Tomonao Matsushita, Yuichiro Watanabe
PLANT CELL AND ENVIRONMENT
(2019)
Article
Plant Sciences
Nyet-Cheng Chiam, Tomoyo Fujimura, Ryosuke Sano, Nobuhiro Akiyoshi, Ryoko Hiroyama, Yuichiro Watanabe, Hiroyasu Motose, Taku Demura, Misato Ohtani
PLANT AND CELL PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Masayuki Tsuzuki, Kazutaka Futagami, Masaki Shimamura, Chikako Inoue, Kan Kunimoto, Takashi Oogami, Yuki Tomita, Keisuke Inoue, Takayuki Kohchi, Shohei Yamaoka, Takashi Araki, Takahiro Hamada, Yuichiro Watanabe
Article
Multidisciplinary Sciences
Masayuki Tsuzuki, Shriya Sethuraman, Adriana N. Coke, M. Hafiz Rothi, Alan P. Boyle, Andrzej T. Wierzbicki
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Microbiology
Pamela Gan, Ryoko Hiroyama, Ayako Tsushima, Sachiko Masuda, Arisa Shibata, Akiko Ueno, Naoyoshi Kumakura, Mari Narusaka, Trinh Xuan Hoat, Yoshihiro Narusaka, Yoshitaka Takano, Ken Shirasu
Summary: This study analyzed the genome features of different pathogenic Colletotrichum strains using comparative genomics, identifying associations between related effector gene clusters and genome organization, as well as evidence of exchange between genomic compartments. Additionally, it showed a tendency for correlated gene expression during infection in orthologous gene clusters.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Plant Sciences
Jinlian Chen, Yoshihiro Inoue, Naoyoshi Kumakura, Kazuyuki Mise, Ken Shirasu, Yoshitaka Takano
Summary: The study revealed that the effectors NLP1 and NIS1 from C. orbiculare show different cell death-inducing activities in different plant species, indicating distinct responses to these effectors in the plants infected by the fungus.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
M. Hafiz Rothi, Masayuki Tsuzuki, Shriya Sethuraman, Andrzej T. Wierzbicki
Summary: Non-coding transcription is crucial for heterochromatin formation, with Pol V in Arabidopsis thaliana transcribing long non-coding RNAs to facilitate locus-specific establishment of RdDM. DNA methylation enhances Pol V transcription, with a mutual reinforcement between DNA methylation and Pol V transcription maintaining RdDM. Loss of DNA methylation at specific loci in certain mutants affects Pol V transcription, indicating a complex crosstalk between RdDM and other silencing pathways.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Mayumi Hori, Katsumi Shozugawa, Kenji Sugimori, Yuichiro Watanabe
Summary: The comprehensive tap water hardness assessment in Japan showed that the water was relatively soft with low-mineral content compared to 27 other countries. Variations in hardness were observed across different regions, with the distribution system's water pipes having minimal impact on tap water hardness.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Ayako Tsushima, Mari Narusaka, Pamela Gan, Naoyoshi Kumakura, Ryoko Hiroyama, Naoki Kato, Shunji Takahashi, Yoshitaka Takano, Yoshihiro Narusaka, Ken Shirasu
Summary: In this study, conserved effectors were identified and characterized in Colletotrichum fungi. One of these effectors, CEC3, was found to induce nuclear expansion and cell death during infection, suggesting a potential role in promoting host cell death. These results indicate that CEC3 proteins could be a novel class of core effectors with functional conservation in the genus Colletotrichum.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Masayuki Tsuzuki, Kanako Inoue, Akinori Kiba, Kouhei Ohnishi, Kenji Kai, Yasufumi Hikichi
Summary: The Gram-negative bacterium Ralstonia pseudosolanacearum strain OE1-1 attaches to tomato roots, activates quorum sensing, and forms biofilms to infect xylem vessels. Quorum sensing involves the induction and activation of the transcriptional regulator PhcA, and is associated with the production of secondary metabolites and exopolysaccharides. These components contribute to the positive feedback loop of quorum sensing.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biology
Mingming Chen, Naoyoshi Kumakura, Hironori Saito, Ryan Muller, Madoka Nishimoto, Mari Mito, Pamela Gan, Nicholas T. Ingolia, Ken Shirasu, Takuhiro Ito, Yuichi Shichino, Shintaro Iwasaki
Summary: A parasitic fungus has been found to overcome the toxicity of antimicrobial compounds produced by plants through a specific amino acid substitution. This study reveals the molecular mechanisms of fungal resistance against plant defense mechanisms.
Article
Plant Sciences
Katsuma Yonehara, Naoyoshi Kumakura, Takayuki Motoyama, Nobuaki Ishihama, Jean-Felix Dallery, Richard O'Connell, Ken Shirasu
Summary: Researchers have developed an efficient method for studying the factors involved in the pathogenicity of Colletotrichum higginsianum, a hemibiotrophic pathogen causing anthracnose disease on crucifer hosts. By combining CRISPR/Cas9 and a URA3-based marker recycling system, they successfully targeted two melanin biosynthesis genes, resulting in mutants with deficient melanization and loss of pathogenicity.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Chika Takemura, Wakana Senuma, Masayuki Tsuzuki, Yuki Terazawa, Kanako Inoue, Masanao Sato, Akinori Kiba, Kouhei Ohnishi, Kenji Kai, Yasufumi Hikichi
Summary: Research shows that the protein ChpA regulates QS-dependent genes and affects the behavior in host plant roots and virulence of pathogenic bacterium Ralstonia pseudosolanacearum OE1-1.
MOLECULAR PLANT PATHOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Halina Pietrykowska, Alisha Alisha, Bharti Aggarwal, Yuichiro Watanabe, Misato Ohtani, Artur Jarmolowski, Izabela Sierocka, Zofia Szweykowska-Kulinska
Summary: Functional regulatory non-coding RNAs (ncRNAs) play a vital role in plant growth and development. The liverwort Marchantia polymorpha serves as an excellent model species for studying the evolution of regulatory ncRNAs and proteins involved in ncRNA biogenesis. Recent studies on Marchantia have revealed the similarities and differences in the production and function of ncRNAs between bryophytes and other land plants. Understanding the molecular mechanisms controlling ncRNA-directed developmental processes requires further functional studies.
PLANT MOLECULAR BIOLOGY
(2023)