Article
Infectious Diseases
Tae Kwon Kim, Jessica Waldman, Freddy Ibanez-Carrasco, Lucas Tirloni, Camila Waltero, Christiano Calixo, Gloria R. Braz, Albert Mulenga, Itabajara da Silva Vaz, Carlos Logullo
Summary: This study identified and selected stable reference genes in the R. microplus genome and evaluated their expression stability during embryogenesis. Results showed that Rpl4, Elf1a, and GAPDH are suitable internal controls for normalizing qPCR during R. microplus embryogenesis.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Endocrinology & Metabolism
Bulent Ayas, Seda Kirmizikan, Adem Kocaman, Bahattin Avci
Summary: The study aimed to investigate the effects of metformin treatment at different dosage levels on rat offspring ovaries and uteruses during the intrauterine period. The results showed that gestational exposure to high dose metformin may lead to decreased estradiol production in the offspring rats, resulting in reduced endometrial thickness.
GYNECOLOGICAL ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Adomar Laurindo Neto, Felipe Lopes Ruas, Bruno Silva Espirito Santo, Ana Clara Degan Mattos, Junior Borges Silva, Marcos Flavio Diniz Jr, Danilo Fonseca Cardoso Pereira, Izabelle Pereira Lacerda, Jose Oliveira Carvalho, Guilherme Pugliesi
Summary: This study tested the impact of supplementing long-acting P4 at different times during the initial dioestrus on the pregnancy rates of dairy and beef recipients undergoing fixed-time embryo transfer. The results showed that using iP4 during FTET can enhance the pregnancy rates of beef recipients, although the effectiveness of iP4 supplementation on pregnancy maintenance may vary under different experimental conditions.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Chemistry, Analytical
Bailey F. McCarthy Riley, Hao T. Mai, Thomas H. Linz
Summary: This report presents a single-liposome analysis method that combines digital PCR and quantitative PCR techniques to measure the copy numbers of DNA within liposomes and assess their heterogeneity. Compared to traditional bulk analysis methods, this new approach reveals more population heterogeneity and can improve the uniformity of liposomal biopharmaceutical formulations.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jan Tesarik
Summary: Antioxidant treatment is increasingly used to slow down aging processes and improve female fertility, but potential overmedication without medical supervision can lead to adverse effects. Further research and personalized prescription are needed for optimal outcomes.
Article
Obstetrics & Gynecology
Alexa Bishop, Judith E. Cartwright, Guy S. Whitley
Summary: Stanniocalcin-1 (STC-1) is a glycoprotein hormone with wide expression in female reproductive tissues, playing a vital role in processes such as ovarian follicular development, blastocyst implantation, and placental development. Dysregulation of STC-1 is associated with severe reproductive disorders, highlighting the need for further research on its regulation and function.
HUMAN REPRODUCTION UPDATE
(2021)
Article
Biochemical Research Methods
Nirmal Kumar Sampathkumar, Venkat Krishnan SundaramID, Prakroothi S. Danthi, Rasha Barakat, Shiden Solomon, Mrityunjoy Mondal, Ivo Carre, Tatiana El Jalkh, Aida Padilla-Ferrer, Julien Grenier, Charbel Massaad, Jacqueline C. Mitchell
Summary: The choice of reference genes heavily influences the assessment of differential gene expression by qPCR. Recent studies suggest using RNA-Seq to identify stable genes and statistical approaches to determine the best reference genes. However, this study demonstrates that the statistical approach to determine the best reference genes from commonly used candidates is more important than preselecting stable genes from RNA-Seq data.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Plant Sciences
Michaela Jung, Beat Keller, Morgane Roth, Maria Jose Aranzana, Annemarie Auwerkerken, Walter Guerra, Mehdi Al-Rifai, Mariusz Lewandowski, Nadia Sanin, Marijn Rymenants, Frederique Didelot, Christian Dujak, Carolina Font i Forcada, Andrea Knauf, Francois Laurens, Bruno Studer, Helene Muranty, Andrea Patocchi
Summary: The study using the multi-environment apple reference population demonstrated genetic architecture and genomic predictive abilities for 30 quantitative traits in apple; GWAS revealed 59 stable and 277 location-specific associations, 69.2% of which were novel; genotype by environment interactions accounted for up to 24% of the phenotypic variability.
HORTICULTURE RESEARCH
(2022)
Article
Genetics & Heredity
Hua Cao, Han Li, Lin Lu, Yulu Ji, Lulin Ma, Shenchong Li
Summary: This study selected and evaluated the suitability of ten candidate RGs for normalizing leaf color-related genes' expression levels via qRT-PCR. The stability rankings analysis disclosed that EF1 alpha exhibited the highest stability and was chosen as the most reliable reference gene. The results offer key genetic resources for the functional characterization of leaf color-related genes and will pave the way for molecular dissection of leaf color mutations in D. officinale.
Review
Veterinary Sciences
Ting-Yu Cheng, Jeffrey J. Zimmerman, Luis G. Gimenez-Lirola
Summary: In qPCR assays for oral fluid specimens, the use of internal reference genes has not been fully explored or validated, which hampers the reliability and reproducibility of the results.
ANIMAL HEALTH RESEARCH REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima
Summary: The study aimed to identify suitable putative internal control genes in milk sEVs for qRT-PCR data normalization. TUB, ETFDH, and ACTB were identified as highly stable putative internal control genes in milk sEVs, enhancing the robustness of qRT-PCR. Their stability in sEVs under normal or healthy conditions suggests their potential as reliable internal control genes for qRT-PCR in bovine milk sEVs.
Article
Environmental Sciences
Kazuyoshi Koike, Ryo Honda, Masataka Aoki, Ryoko Yamamoto-Ikemoto, Kazuaki Syutsubo, Norihisa Matsuura
Summary: In this study, internal standard genes (ISGs) were developed and added to samples for amplicon sequencing, allowing for the conversion of read counts into absolute gene numbers. The method was applied to environmental samples, enabling analysis of phylogeny, functional diversity, and absolute quantities of genes.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Saqib Rahman, Zhenzhen Zhao, Muhammad Umair Sial, Yanning Zhang, Hongyun Jiang
Summary: This study confirmed the stability of Actin and 18S as reference genes for normalizing the expression of target genes in Myzus persicae under different conditions. Both genes were effective in normalizing the expression levels of target genes under insecticide treatment and starvation. However, Actin was more stable than 18S at the developmental stages of M. persicae.
Article
Agriculture, Dairy & Animal Science
Ola Adel, Sally Ibrahim, Karima Mahmoud, Samaa M. Galal, Mohamed Fathi, Adel A. M. Seida
Summary: This study investigated gene expression profiles in pregnant and non-pregnant she-camels and found that relevant genes to uterine extra-cellular matrix remodeling (ITGB4, SLCO2A1, FOS, and JUN), uterine tissue vascularization and placental formation (VEGFA, PGF, and PDGFA), embryonic growth and development (IGF1 and PTEN), as well as cell death of uterine tissue (BCL2), were up-regulated in pregnant she-camels compared to non-pregnant ones. In addition, higher progesterone levels and lower estradiol17-beta levels were observed in pregnant she-camels. This suggests that precise regulation of genes related to implantation, matrix formation, vascularization, and placental formation is crucial for successful pregnancy in she-camels.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Multidisciplinary Sciences
Jing Tong, Manman Hu, Beibei Han, Yanhai Ji, Baoju Wang, Hao Liang, Mingchi Liu, Zhanhui Wu, Ning Liu
Summary: Chinese chive has unique flavor, nutrient, and medicinal values, and investigating its molecular mechanism on flavor formation and metabolic pathways is crucial. This study identified GLY1 and SKP1 as the most stable reference genes for normalization, highlighting the importance of validating reference gene stability before use. Using at least two reference genes for normalization can provide more accurate results, with the combination of GLY1 and SKP1 showing reliable normalization results in qRT-PCR experiments.
SCIENTIFIC REPORTS
(2021)
Article
Gastroenterology & Hepatology
Daniel Sepulveda-Crespo, Ana Trevino-Nakoura, Jose M. Bellon, Beatriz Ardizone Jimenez, Maria A. Jimenez-Sousa, Amanda Fernandez-Rodriguez, Isidoro Martinez, Salvador Resino
Summary: The diagnostic performance of the HCVcAg assay in monitoring the efficacy of HCV treatment with DAAs is poor at weeks 2 and 4, fair at end-of-treatment, and excellent for sustained virological response.
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Microbiology
Daniel Sepulveda-Crespo, Ana Trevino-Nakoura, Jose M. Bellon, Maria A. Jimenez-Sousa, Pablo Ryan, Isidoro Martinez, Amanda Fernandez-Rodriguez, Salvador Resino
Summary: The standard algorithm for diagnosing hepatitis C virus (HCV) infection involves an HCV antibody test for screening and a nucleic acid amplification test (NAAT) for confirmation. The accuracy of the Abbott ARCHITECT HCV Ag assay was evaluated in people living with HIV/AIDS (PLWHA), showing good performance in high-prevalence settings but poor in low-prevalence settings. It was excellent in excluding active HCV infection.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Virology
Ana Virseda-Berdices, Leyre Concostrina-Martinez, Oscar Martinez-Gonzalez, Rafael Blancas, Salvador Resino, Pablo Ryan, Maria Martin-Vicente, Oscar Brochado-Kith, Natalia Blanca-Lopez, Maria J. Mallol Poyato, Blanca Lopez Matamala, Carmen Martin Parra, Maria A. Jimenez-Sousa, Amanda Fernandez-Rodriguez
Summary: Increasing age is associated with severity and higher mortality of COVID-19. Telomere shortening is associated with higher risk of infections and may be used to identify those patients who are more likely to die. This study evaluated the association between relative telomere length (RTL) and COVID-19 mortality. Lower RTL was associated with a higher risk of death in women at 30 and 90 days.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Medicine, General & Internal
J. Castilla, M. Garcia Cenoz, R. Abad, L. Sanchez-Cambronero, N. Lorusso, C. Izquierdo, S. Canellas Llabres, J. Roig, A. Malvar, F. Gonzalez Carril, A. L. D. Boone, J. Perez Martin, M. J. Rodriguez Recio, A. Galmes, A. Caballero, A. Garcia Rojas, F. Juanas, M. Nieto, L. J. Viloria Raymundo, E. Martinez Ochoa, A. Rivas, D. Castrillejo, D. Moreno Perez, A. Martinez, E. Borras, A. Sanchez Gomez, E. Pastor, V Nartallo, J. M. Arteagoitia, B. Alvarez-Fernandez, R. Garcia Pina, S. Fernandez Arribas, J. Vanrell, S. Garcia Hernandez, R. M. Mendoza, M. Mendez, M. M. Lopez-Tercero, A. Fernandez-Rodriguez, A. Blanco, F. J. Carrillo de Albornoz, J. Ruiz Olivares, R. Ruiz-Montero, A. Limia, J. A. Navarro-Alonso, J. A. Vazquez, A. Barricarte
Summary: This study assessed the effectiveness of a protein-based meningococcal vaccine in preventing invasive meningococcal disease in Spanish children. The results showed that complete vaccination with the vaccine was 76% effective against invasive meningococcal disease caused by any serogroup, and partial vaccination was 54% effective.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Medicine, Research & Experimental
Sergio Salguero, Oscar Brochado-Kith, Ana Virseda Verdices, Juan Berenguer, Juan Gonzalez-Garcia, Isidoro Martinez, Cristina Diez, Victor Hontanon, Leire Perez-Latorre, Amanda Fernandez-Rodriguez, Maria Angeles Jimenez-Sousa, Salvador Resino
Summary: We identified a gene expression signature associated with advanced cirrhosis with high risk for clinically significant portal hypertension (CSPH) in HIV/HCV coinfected patients through analyzing the gene expression profile of peripheral blood mononuclear cells (PBMCs). A cross-sectional study on 68 patients was conducted, and liver stiffness measurement (LSM) was used for stratification. We found 60 differentially expressed transcripts between patients with LSM < 12.5 kPa and >= 25 kPa, and two of them were validated in a different cohort.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Violeta Lara-Aguilar, Daniel Valle-Millares, Celia Crespo-Bermejo, Sergio Grande-Garcia, Manuel Llamas-Adan, Maria Engracia Cortijo-Alfonso, Luz Martin-Carbonero, Lourdes Dominguez, Pablo Ryan, Ignacio de los Santos, Sofia Bartolome-Sanchez, Erick Joan Vidal-Alcantara, Maria Angeles Jimenez-Sousa, Amanda Fernandez-Rodriguez, Veronica Briz
Summary: This study examined the senescence profiles in people living with HIV who have acute or chronic Hepatitis C. The results showed that the profiles improved after HCV elimination, either spontaneously or with direct-acting antivirals.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Erola Pairo-Castineira, Konrad Rawlik, Andrew D. Bretherick, Ting Qi, Yang Wu, Isar Nassiri, Glenn A. McConkey, Marie Zechner, Lucija Klaric, Fiona Griffiths, Wilna Oosthuyzen, Athanasios Kousathanas, Anne Richmond, Jonathan Millar, Clark D. Russell, Tomas Malinauskas, Ryan Thwaites, Kirstie Morrice, Sean Keating, David Maslove, Alistair Nichol, Malcolm G. Semple, Julian Knight, Manu Shankar-Hari, Charlotte Summers, Charles Hinds, Peter Horby, Lowell Ling, Danny McAuley, Hugh Montgomery, Peter J. M. Openshaw, Colin Begg, Timothy Walsh, Albert Tenesa, Carlos Flores, Jose A. Riancho, Augusto Rojas-Martinez, Pablo Lapunzina, Jian Yang, Chris P. Ponting, James F. Wilson, Veronique Vitart, Malak Abedalthagafi, Andre D. Luchessi, Esteban J. Parra, Raquel Cruz, Angel Carracedo, Angie Fawkes, Lee Murphy, Kathy Rowan, Alexandre C. Pereira, Andy Law, Benjamin Fairfax, Sara Clohisey Hendry, J. Kenneth Baillie
Summary: This study analyzed genetic data from 24,202 severe COVID-19 cases and identified potentially druggable targets, including inflammatory signaling, monocyte-macrophage activation and endothelial permeability, immunometabolism, and host factors required for viral entry and replication.
Review
Virology
Daniel Sepulveda-Crespo, Ana Trevino-Nakoura, Jose M. Bellon, Amanda Fernandez-Rodriguez, Pablo Ryan, Isidoro Martinez, Maria A. Jimenez-Sousa, Salvador Resino
Summary: This meta-analysis evaluated the diagnostic performance of the Abbott ARCHITECT HCV Ag assay for diagnosing active hepatitis C. The assay showed excellent sensitivity, specificity, and accuracy. However, its diagnostic utility may be limited in low prevalence settings, and further confirmation is recommended.
REVIEWS IN MEDICAL VIROLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Rafael Suarez-Mesa, Roger Ros-Freixedes, Houda Laghouaouta, Ramona N. Pena, Byron Hernandez-Ortiz, Iang Rondon-Barragan, Joan Estany
Summary: Dissecting genetic variation is important for conservation success. This study investigated the genomic variation of Colombian Creole pigs and identified breed-specific variants in genes related to adaptive and economic traits. The findings suggest that Colombian Creole pigs are not exempt from selective introgression of other breeds and provide insights for effective breeding and conservation.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Psychiatry
M. Alemany-Navarro, S. Diz-de Almeida, R. Cruz, J. A. Riancho, A. Rojas-Martinez, P. Lapunzina, C. Flores, A. Carracedo
Summary: Despite individual variation in the clinical presentation of COVID-19, schizophrenia patients show a higher risk and severity of the disease compared to control counterparts. The genetic risk for schizophrenia, but not for bipolar disorder or depression, is associated with a higher risk of SARS-CoV-2 infection and COVID-19 severity, especially among women. However, the predictive accuracy is limited.
TRANSLATIONAL PSYCHIATRY
(2023)
Correction
Medicine, Research & Experimental
Sergio Salguero, Oscar Brochado-Kith, Ana Virseda Verdices, Juan Berenguer, Juan Gonzalez-Garcia, Isidoro Martinez, Cristina Diez, Victor Hontanon, Leire Perez-Latorre, Amanda Fernandez-Rodriguez, Maria Angeles Jimenez-Sousa, Salvador Resino
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Ruben Martin-Escolano, Ana Virseda-Berdices, Juan Berenguer, Juan Gonzalez-Garcia, Oscar Brochado-Kith, Amanda Fernandez-Rodriguez, Cristina Diez, Victor Hontanon, Salvador Resino, Maria Angeles Jimenez-Sousa
Summary: This study aimed to evaluate the association between plasma immune checkpoint proteins at baseline with hepatic steatosis index increase at the end of follow-up in HIV/HCV-coinfected patients. The results showed that higher levels of immune checkpoint proteins BTLA, CD137, CD80, GITR, LAG-3, and PD-L1 before HCV therapy were associated with a long-term increase in hepatic steatosis index after successful HCV therapy, suggesting a potential predictive role for early detection of progression towards steatosis in HIV/HCV-coinfected patients.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Virology
Violeta Lara-Aguilar, Celia Crespo-Bermejo, Manuel Llamas-Adan, Sergio Grande-Garcia, Maria Engracia Cortijo-Alfonso, Luz Martin-Carbonero, Lourdes Dominguez, Pablo Ryan, Ignacio de los Santos, Sofia Bartolome-Sanchez, Daniel Valle-Millares, Maria Angeles Jimenez-Sousa, Veronica Briz, Amanda Fernandez-Rodriguez
Summary: Coinfection with HCV and HIV leads to immune activation, inflammation, oxidative stress, and premature senescence. Chronic HCV infection in PLWHIV is associated with increased nitrate concentration and secretion of senescence-associated factors.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Cell Biology
Patricia Castro-Santos, Augusto Rojas-Martinez, Jose A. Riancho, Pablo Lapunzina, Carlos Flores, Angel Carracedo, Roberto Diaz-Pena, Scourge Cohort Group
Summary: We investigated the relationship between HLA polymorphisms and susceptibility to SARS-CoV-2 infection and disease severity. Genotyping data from 9373 COVID-19-positive cases and 5943 population controls were analyzed. The study found associations between HLA-B*14:02 and HLA-C*08:02 alleles with a lower risk of COVID-19 infection, and HLA-A*11:01 and HLA-C*04:01 alleles with disease severity.
Article
Agriculture, Dairy & Animal Science
Houda Laghouaouta, Lorenzo Fraile, Rafael Suarez-Mesa, Roger Ros-Freixedes, Joan Estany, Ramona Natacha Pena
Summary: This study provides new insights into the genetic make-up of resilience in growing pigs by identifying genomic regions and candidate genes associated with resilience indicators. The findings suggest that there are novel genomic regions associated with two resilience indicators (increment BW and increment HP) in pigs, which harbour potential candidate genes involved in immune response and growth pathways, highlighting the strong relationship between resilience and immune response.
GENETICS SELECTION EVOLUTION
(2022)