Article
Multidisciplinary Sciences
Nandhini Rajagopal, Shikha Nangia
Summary: This study investigates the molecular assembly of claudins, specifically claudin-5 and claudin-15, to determine their structural and functional properties. The research findings suggest that each member of the claudin family has a unique strand architecture that requires systematic molecular-level analysis.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Physiology
Alejandro E. Garcia-Flores, Christine M. Gross, Evgeny A. Zemskov, Qing Lu, Kim Tieu, Ting Wang, Stephen M. Black
Summary: Mechanical strain contributes to ventilator-induced lung injury through the loss of pulmonary endothelial tight junctions. Overexpression of SOX18 can protect endothelial barrier function and attenuate inflammatory lung injury. Enhancing SOX18 expression may prove a useful therapy to treat patients with ventilator-induced lung injury.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Oliver Brookes, Sonja Boland, Rene Lai Kuen, Dorian Miremont, Jamileh Movassat, Armelle Baeza-Squiban
Summary: Research on the development of an air-liquid interface model of alveolar epithelium incorporating features of type I and type II epithelial cells showed promising results in terms of cell morphology and barrier function. The coculture model demonstrated stable barrier properties similar to native alveolar epithelium, indicating its potential for representing a broader range of functions.
Article
Cell Biology
Daniel Maucher, Birte Schmidt, Julia Schumann
Summary: Dysfunction of the endothelial barrier is central in inflammatory processes like sepsis and atherosclerosis. In this study, cytokine-induced weakening of the endothelial barrier was associated with changes in the transcriptome and upregulation of miR-29a-3p, miR-29b-3p, and miR-155-5p, which target central adhesion proteins' mRNAs. This suggests a posttranscriptional mechanism regulated by inflammatory cytokines.
Article
Microbiology
Kenneth Smith, Lori Garman, Kathleen Norris, Jennifer Muther, Angie Duke, Renata J. M. Engler, Michael R. Nelson, Limone C. Collins, Christina Spooner, Carla Guthridge, Judith A. James
Summary: Research has found that some individuals vaccinated with anthrax vaccine may produce antibodies with poor neutralizing capabilities, potentially due to increased IgG4 to IgG1 ratios, low antibody avidity, and insufficient antibody targeting specific regions. Future vaccine formulations and vaccination schedules should aim to improve these deficiencies.
Article
Multidisciplinary Sciences
Laura-Sophie Beier, Ayk Waldow, Saeed Khomeijani Farahani, Roman Mannweiler, Sabine Vidal-Y-Sy, Johanna M. Brandner, Jorg Piontek, Dorothee Gunzel
Summary: The formation of tight junction (TJ) is crucial for epidermal barrier function. Manipulation of TJ barriers in reconstructed human epidermis (RHE) was achieved through knockdown of claudin-1 and -4 or by using claudin-binding fusion proteins. The study demonstrates the critical role of claudin-1 in regulating paracellular permeability in the viable epidermis.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Joel Brunner, Sakthikumar Ragupathy, Gerrit Borchard
Summary: Research focuses on modulating intercellular tight junctions to enhance drug absorption and bioavailability, with challenges in commercialization. Target-specific permeation enhancers have potential clinical applications.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Pharmacology & Pharmacy
Simona Federica Spampinato, Sara Merlo, Yasuteru Sano, Takashi Kanda, Maria Angela Sortino
Summary: Sphingosine 1 phosphate (S1P) is a bioactive sphingolipid with important physiological and pathological functions. The drug siponimod (BAF-312) has been approved for secondary progressive MS treatment by targeting S1P1 and S1P5 receptors, showing potential to strengthen BBB properties and provide additional effects for MS treatment.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Cell Biology
Peter Baluk, Keisuke Shirakura, Dietmar Vestweber, Donald M. Mcdonald
Summary: Endothelial cells of mammalian blood vessels exhibit multiple levels of heterogeneity along the vascular tree and among different organs, and further heterogeneity is caused by blood flow turbulence and variations in shear stress. This study discovered new levels of functional mosaicism in the endothelial cells of the aorta and vena cava, where blood flow dynamics are known to be heterogeneous.
CELL AND TISSUE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Alessandro Berselli, Giulio Alberini, Fabio Benfenati, Luca Maragliano
Summary: Tight junctions (TJs) are multi-protein complexes that seal the paracellular space between adjacent endothelial or epithelial cells. The Claudin-5 (Cldn5) protein plays a crucial role in the blood-brain barrier (BBB) and little is known about its TJ assemblies. In this study, molecular dynamics simulations were used to investigate the permeation of ions and water through different Cldn5 structures, including a pathogenic mutation. The findings provide insights into the selectivity of BBB's paracellular transport and validate the structural models.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Review
Multidisciplinary Sciences
Erika Wakayama, Taiki Kuzu, Keisuke Tachibana, Ryuichi Hirayama, Yoshiaki Okada, Masuo Kondoh
Summary: The blood-brain barrier poses a challenge to drug delivery to the central nervous system. Among the components of the tight junctions in the blood-brain barrier, claudin-5 has been studied extensively as a target for enhancing drug permeation into the brain. However, sustained knockdown of claudin-5 is lethal in mice and administration of an anti-claudin-5 antibody can cause convulsions in nonhuman primates.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Desislava Kolchakova, Dzhemal Moten, Tsvetelina Batsalova, Balik Dzhambazov
Summary: Claudins play a crucial role in cell migration and metastasis, making them potential therapeutic targets for cancer treatment. Experimental results show that anti-claudin-12 antibodies and inhibitory peptides can significantly reduce the migration and proliferation of claudin-12 expressing cells, suggesting their potential in inhibiting cancer cell migration.
Article
Immunology
Umida Ganieva, Sylvia Schneiderman, Pengli Bu, Kenneth Beaman, Svetlana Dambaeva
Summary: IL-22 plays a crucial role in the regeneration of the uterine endometrium after inflammation-triggered abortion. IL-22 deficiency may be associated with secondary infertility and delayed uterine clearance, possibly mediated by the regulation of tight junction proteins and epithelial-mesenchymal transition markers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biophysics
Minghan Li, Shanchuan Chen, Yunjiao Wang, Shaoxia Zhang, Dandan Song, Rong Tian, Jia Geng, Liang Wang
Summary: In this study, a nanopore platform was fabricated to monitor intercellular protein transport and detect the activity of anthrax lethal factor (aLF) at a single molecule level. The aLF activity was successfully inhibited using screening approaches of protein-metal ion acquisition and other condition controlment. Ca2+, Mg2+, Mn2+, Ni2+ in collaboration with Zn2+ were found to promote aLF activity, while Cd2+, Co2+, Cu2+ showed inhibitory effects. The results also revealed that the speed of aLF endopeptidase activity with different ions functions as the nanopore signal frequency in a linear manner. In addition, the strength of the proton or hydroxide, as well as adapted temperature and gamma-ray, played integral roles in inhibiting aLF activity.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Gastroenterology & Hepatology
Roberta Lais Mendonca de Mattos, Danilo Toshio Kanno, Fabio Guilherme Campos, Geovanna Pacciulli Pereira, Mateus Magami Yoshitani, Andress de Godoy Delben, Jose Aires Pereira, Carlos Augusto Real Martinez
Summary: The study found that the content of claudin-3 and occludin in the cell membranes of neoplastic tissue in patients with colorectal cancer is reduced.
JOURNAL OF GASTROINTESTINAL SURGERY
(2022)
Article
Cell Biology
Allison J. Greaney, Nolan K. Maier, Stephen H. Leppla, Mahtab Moayeri
JOURNAL OF LEUKOCYTE BIOLOGY
(2016)
Article
Microbiology
Annabel Guichard, Prashant Jain, Mahtab Moayeri, Ruth Schwartz, Stephen Chin, Lin Zhu, Beatriz Cruz-Moreno, Janet Z. Liu, Bernice Aguilar, Andrew Hollands, Stephen H. Leppla, Victor Nizet, Ethan Bier
Article
Immunology
Pan Tao, Marthandan Mahalingam, Jingen Zhu, Mahtab Moayeri, Michelle L. Kirtley, Eric C. Fitts, Jourdan A. Andersson, William S. Lawrence, Stephen H. Leppla, Ashok K. Chopra, Venigalla B. Rao
FRONTIERS IN IMMUNOLOGY
(2017)
Article
Chemistry, Medicinal
Guan-Sheng Jiao, Seongjin Kim, Mahtab Moayeri, April Thai, Lynne Cregar-Hernandez, Linda McKasson, Sean O'Malley, Stephen H. Leppla, Alan T. Johnson
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2018)
Article
Immunology
Catherine E. Vrentas, Robert G. Schaut, Paola M. Boggiatto, Steven C. Olsen, Fayyaz S. Sutterwala, Mahtab Moayeri
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2018)
Article
Immunology
Eduardo P. Amaral, Nicolas Riteau, Mahtab Moayeri, Nolan Maier, Katrin D. Mayer-Barber, Rosana M. Pereira, Silvia L. Lage, Andre Kubler, William R. Bishai, Maria R. D'Imperio-Lima, Alan Sher, Bruno B. Andrade
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Microbiology
Pan Tao, Marthandan Mahalingam, Jingen Zhu, Mahtab Moayeri, Jian Sha, William S. Lawrence, Stephen H. Leppla, Ashok K. Chopra, Venigalla B. Rao
Article
Cardiac & Cardiovascular Systems
Xizhong Cui, Wanying Xu, Pranita Neupane, Andie Weiser-Schlesinger, Ray Weng, Benjamin Pockros, Yan Li, Mahtab Moayeri, Stephen H. Leppla, Yvonne Fitz, Peter Q. Eichacker
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Catherine E. Vrentas, Paola M. Boggiatto, Steven C. Olsen, Stephen H. Leppla, Mahtab Moayeri
Article
Cell Biology
Allison J. Greaney, Makayla K. Portley, Danielle O'Mard, Devorah Crown, Nolan K. Maier, Megan A. Mendenhall, Katrin D. Mayer-Barber, Stephen H. Leppla, Mahtab Moayeri
JOURNAL OF LEUKOCYTE BIOLOGY
(2020)
Article
Microbiology
Megan A. Mendenhall, Shihui Liu, Makayla K. Portley, Danielle O'Mard, Rasem Fattah, Roman Szabo, Thomas H. Bugge, Jaspal S. Khillan, Stephen H. Leppla, Mahtab Moayeri
NATURE MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Carly Merritt, Elizabeth M. Chun, Rasem J. Fattah, Lakmali M. Silva, Quinn Q. Ma, Mahtab Moayeri, Dennis Paliga, Sebastian Neumann, Rolf Heumann, Stephen H. Leppla, Thomas H. Bugge
Summary: This study presents a method to visualize anthrax toxin intoxication in animals and reveals the differential roles of CMG-2 and TEM-8 receptors in intoxication of different organs. It also demonstrates that CMG-2 is the dominant receptor on leukocytes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Immunology
Anita Verma, Mahtab Moayeri, Christopher P. Mocca, Danielle O'Mard, Qian Ma, Stephen H. Leppla, Drusilla L. Burns
Summary: The study reveals that the CMG2 receptor plays a predominant role in mediating the toxin-neutralizing antibody response to anthrax protective antigen (PA), while the TEM8 receptor has a weaker effect.
Article
Cardiac & Cardiovascular Systems
Dante A. Suffredini, Yan Li, Wanying Xu, Mahtab Moayeri, Stephen Leppla, Yvonne Fitz, Xizhong Cui, Peter Q. Eichacker
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2017)
Article
Microbiology
Andrea B. Keefer, Eugenia K. Asare, Andrei P. Pomerantsev, Mahtab Moayeri, Craig Martens, Stephen F. Porcella, Susan Gottesman, Stephen H. Leppla, Catherine E. Vrentas