Article
Environmental Sciences
Jiayuan Zhang, Miao Zhang, Jian Zhang, Fang Wang, Yingchun Wang, Linlin Zheng
Summary: In this study, the syntaxin gene RtSYP121 was isolated and functionally characterized from Reaumuria trigyna. It was found that RtSYP121 can enhance the Cd tolerance of plants by regulating vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Biochemistry & Molecular Biology
Hyelim Jeon, Cecile Segonzac
Summary: The endomembrane system plays a critical role in the plant response to pathogen infection by supporting the synthesis and transport of immunity-related proteins and antimicrobial compounds. During bacterial infection in plant cells, major modifications occur in the endomembrane system. This review highlights the dynamics of these cellular compartments and their essential contributions to plant defense responses, as well as their emerging roles in bacterial pathogenicity, particularly focusing on the secreted effectors of Pseudomonas syringae, Xanthomonas spp., and Ralstonia solanacearum.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Review
Biochemistry & Molecular Biology
Ilaria Cela, Beatrice Dufrusine, Claudia Rossi, Alberto Luini, Vincenzo De Laurenzi, Luca Federici, Michele Sallese
Summary: KDELRs are ubiquitous receptors involved in cell quality control and stress responses. Mutations in KDELRs are associated with various diseases, making them potential therapeutic targets.
Review
Cell Biology
Yiran Sun, Chenchen Yan, Libo He, Shixie Xiang, Pan Wang, Zhonghua Li, Yuanzhao Chen, Jie Zhao, Ye Yuan, Wang Wang, Xiaowei Zhang, Pan Su, Yunfang Su, Jinlian Ma, Jiangyan Xu, Quekun Peng, Huifen Ma, Zhishen Xie, Zhenqiang Zhang
Summary: Alzheimer's disease (AD), a chronic and progressive neurodegenerative disease, poses a serious threat to the health of the elderly. The microscopic features of the AD brain are amyloid plaques and neurofibrillary tangles. Despite extensive research, there are still no effective drugs to halt the progression of AD. Ferroptosis, a type of programmed cell death, has been shown to promote the development of AD, and inhibiting neuronal ferroptosis can improve cognitive impairment. Calcium (Ca2+) dyshomeostasis has been found to be closely related to AD pathology, driving ferroptosis through various pathways. This paper reviews the roles of ferroptosis and Ca2+ in AD pathology and suggests that targeting the homeostasis of Ca2+ to restrain ferroptosis may be an innovative approach for AD treatment.
AGEING RESEARCH REVIEWS
(2023)
Editorial Material
Cell Biology
Hana Popelka, Daniel J. J. Klionsky
Summary: A recent study used cryo-electron tomography combined with computational analysis to gain insights into autophagosome biogenesis in yeast cells. This approach provided new information on autophagic structures, their contacts with organelles, membrane sources, and transition mechanisms. These findings open new avenues for autophagy research and highlight the potential of cryo-ET in cell biology.
Article
Plant Sciences
Fangfang Niu, Changyang Ji, Zizhen Liang, Rongfang Guo, Yixuan Chen, Yonglun Zeng, Liwen Jiang
Summary: ADP-ribosylation factor (ARF) family proteins play a central role in regulating vesicular traffic and organelle structures in eukaryotes. This study characterized the function of a unique ARF protein, ARFD1B, in Arabidopsis. It was found that ARFD1B is required for cell plate formation, maintenance of Golgi morphology, and plant growth in Arabidopsis by interacting with COAT PROTEIN COMPLEX I components.
Article
Multidisciplinary Sciences
Kwontae You, Lingfei Wang, Chih-Hung Chou, Kai Liu, Toru Nakata, Alok Jaiswal, Junmei Yao, Ariel Lefkovith, Abdifatah Omar, Jacqueline G. Perrigoue, Jennifer E. Towne, Aviv Regev, Daniel B. Graham, Ramnik J. Xavier
Summary: This study used single-cell RNA sequencing and genome-scale CRISPR screening to reveal the transcriptional states and functional roles associated with adaptive and terminal UPR in the mouse intestinal epithelium. It identified QRICH1 as a key effector of the UPR pathway, controlling a transcriptional program associated with translation and secretory networks crucial for cell fate in response to pathological ER stress.
Review
Plant Sciences
Stephan Clemens
Summary: Nearly 10% of plant proteins belong to the zinc proteome and require zinc as a catalyst or structural element, with the majority of protein-bound zinc in eukaryotic cells found in the cytosol. Various metabolites and peptides serve as zinc buffers to supply zinc to enzymes, transporters, or sensor proteins, with zinc acquisition mediated by specific proteins and zinc transportation controlled by different mechanisms. Recent studies in mammalian cells have revealed the regulatory roles of zinc in modulating cellular zinc pools and influencing protein interactions and signaling cascade activities.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Yaobin Liu, Rukmini Mukherjee, Florian Bonn, Thomas Colby, Ivan Matic, Marius Glogger, Mike Heilemann, Ivan Dikic
Summary: The SidE family of Legionella effectors, particularly SdeA, catalyzes non-canonical phosphoribosyl-linked ubiquitination of host proteins to disrupt Golgi integrity. This disruption prevents Golgi linking proteins GRASP55 and GRASP65 from clustering and forming oligomeric structures on multiple serine residues, impacting the host secretory pathway. The Golgi manipulation strategy by Legionella highlights how the bacterium hijacks the secretory pathway to promote bacterial infection.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biochemistry & Molecular Biology
Richard Van Krieken, Yuan-Li Tsai, Anthony J. Carlos, Dat P. Ha, Amy S. Lee
Summary: Recent research has revealed that ER chaperones such as GRP78 can escape from the ER and traffic to the cell surface in colon and lung cancer via a non-traditional endosomal transport pathway mediated by Rab GTPases. This unconventional translocation mechanism is driven by membrane fusion between ER-derived vesicles and endosomes, requiring specific SNARE proteins and co-chaperones regulated by ER stress-induced signaling pathways.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Oncology
Linlin Zhang, Furong Yan, Liyang Li, Huirong Fu, Dongli Song, Duojiao Wu, Xiangdong Wang
Summary: The communication between endoplasmic reticulum (ER) and mitochondria (Mt) is crucial for maintaining cellular microenvironments, metabolisms, signaling activities, and cell-cell communication. Understanding the molecular mechanisms of ER-Mt communication is essential for identifying and developing specific biomarkers and therapeutic targets for human diseases. The inhibitors and modulators of ER-Mt communication are categorized according to therapeutic targets, and advancements in biotechnologies will provide new insights into the molecular mechanisms of ER-Mt communication.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Danette Kowal Seiler, Jesse C. Hay
Summary: Fluorescent proteins (FPs) have become essential tools for studying the constitutive secretory pathway in live cells since their discovery over fifty years ago. Genetically encoded chemical tags and innovative methods have also contributed to the understanding of sequential trafficking steps in the secretory pathway.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Cell Biology
Ana Catarina Pereira, Nuno Madeira, Sofia Morais, Antonio Macedo, Maria Teresa Cruz, Claudia M. F. Pereira
Summary: This study investigates the role of SERCA inhibition on NLRP3 inflammasome activation in human monocytes. The findings suggest that SERCA inhibition does not activate the NLRP3 inflammasome in innate immune cells. Additionally, the study reveals that SERCA inhibition increases mitochondrial calcium content and fusion, preventing NLRP3 inflammasome activation.
Article
Oncology
Michael P. Ludwig, Matthew D. Galbraith, Neetha Paul Eduthan, Amanda A. Hill, Michael R. Clay, Cristiam Moreno Tellez, Breelyn A. Wilky, Anthony Elias, Joaquin M. Espinosa, Kelly D. Sullivan
Summary: Liposarcoma is a common soft-tissue sarcoma with potential therapeutic targets such as MDM2 and CDK4. This study focuses on the response of liposarcoma to the MDM2 inhibitor nutlin-3, and identifies the involvement of proteostasis network nodes in the mechanism. The combination of nutlin-3 and proteasome inhibitors shows promising apoptosis induction, with the ATF4/CHOP stress response axis playing a crucial role. Activation of the unfolded protein response enhances the efficacy of nutlin-3. In vivo models further confirm the synergistic effects of idasanutlin and carfilzomib in liposarcoma growth.
Article
Biochemistry & Molecular Biology
Leena Haataja, Anoop Arunagiri, Anis Hassan, Kaitlin Regan, Billy Tsai, Balamurugan Dhayalan, Michael A. Weiss, Ming Liu, Peter Arvan
Summary: Efficient export of proinsulin from the endoplasmic reticulum requires the formation of specific disulfide bonds, while disrupting certain pairings may improve trafficking in some mutants. Mutations causing Mutant INS gene-induced Diabetes of Youth (MIDY) often result in perturbed proinsulin disulfide bond formation, with potential for allele-specific pharmacological targeting in the future.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
M. Zhang, X. Zhu, Y. Zhang, Y. Cai, J. Chen, S. Sivaprakasam, A. Gurav, W. Pi, L. Makala, J. Wu, B. Pace, D. Tuan-Lo, V. Ganapathy, N. Singh, H. Li
CELL DEATH AND DIFFERENTIATION
(2015)
Article
Biochemistry & Molecular Biology
K. Kramer, J. Wu, D. L. Crowe
Article
Oncology
Jianchun Wu, David L. Crowe
Article
Genetics & Heredity
Yafei Cai, Wenhu Pi, Satish Sivaprakasam, Xiaobin Zhu, Mingsheng Zhang, Jijun Chen, Levi Makala, Chunwan Lu, Jianchu Wu, Yong Teng, Betty Pace, Dorothy Tuan, Nagendra Singh, Honglin Li
Article
Oncology
Angelica M. Lagunas, Jianchun Wu, David L. Crowe
Meeting Abstract
Oncology
Angelica M. Lagunas, Jianchun Wu, David L. Crowe
Meeting Abstract
Oncology
Jianchun Wu, David L. Crowe
Article
Cell Biology
Jianchun Wu, Hai Jiang, Shouqing Luo, Mingsheng Zhang, Yinghua Zhang, Fei Sun, Shuang Huang, Honglin Li
Article
Oncology
Rowena Wan, Jianchun Wu, Kaitrin K. Baloue, David L. Crowe
INTERNATIONAL JOURNAL OF ONCOLOGY
(2013)
Article
Oncology
Jianchun Wu, David L. Crowe
INTERNATIONAL JOURNAL OF CANCER
(2019)
Article
Oncology
Angelica M. Lagunas, Marybeth Francis, Nisha B. Maniar, Gergana Nikolova, Jianchun Wu, David L. Crowe
MOLECULAR CANCER RESEARCH
(2019)
Article
Oncology
Jianchun Wu, David L. Crowe
MOLECULAR CANCER RESEARCH
(2020)
Meeting Abstract
Oncology
Jianchun Wu, David L. Crowe
Meeting Abstract
Oncology
J. Wu, D. Crowe
Meeting Abstract
Oncology
D. Crowe, K. Kramer, J. Wu