Article
Chemistry, Applied
Li Zhong, Rui Wang, Qin-Hui Wen, Jian Li, Jia-Wei Lin, Xin-An Zeng
Summary: The study found that gingerols can form stable complexes with BSA, significantly improving their solubility and stability. By binding with BSA, gingerols enhanced their solubility and antioxidant capacity, providing a promising strategy for enhancing the bioavailability and efficacy of gingerols.
Review
Biochemistry & Molecular Biology
Xinhao Xu, Jinyu Hu, Huaqian Xue, Yingying Hu, Ya-nan Liu, Guanyang Lin, Liangle Liu, Ren-ai Xu
Summary: This paper reviews the applications of human and bovine serum albumin in biomedical engineering, including their structural differences, extraction methods, characteristics, functions, applications, and future research directions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Xu Li, Yue Yuan, Rui Zhao, Di Shao, Shuyun Bi
Summary: The study investigated the interaction characteristics of marbofloxacin with bovine and human serum albumins, finding that the complex formation was characterized by static quenching with high binding constants. The binding site of marbofloxacin on the proteins was identified to be at site I, driven primarily by hydrogen bonds and van der Waals forces.
Article
Chemistry, Analytical
Sung-Bae Kim, Genta Kamiya, Tadaomi Furuta, Nobuo Kitada, Shojiro A. Maki
Summary: Synthetic coelenterazine (CTZ) indicators were developed to quantitatively study human and bovine serum albumins (HSA and BSA) with high specificity. The CTZ indicators emit luminescence with specific modifications, and their colors and peak positions can determine the origins and mixing levels of albumins. The study also found that serum albumins catalyze the CTZ indicators and this catalytic reaction can be efficiently inhibited by specific inhibitors.
Article
Chemistry, Multidisciplinary
Jialiang Li, Jiqiang Fan, Yan Gao, Shuodan Huang, Di Huang, Jiachen Li, Xiaoyu Wang, Helder A. Santos, Pingping Shen, Bing Xia
Summary: The development of mitochondria-targeted bovine serum albumins (BSAs) conjugated with fluorescent, lipophilic, and cationic rhodamine 110 molecules enhances the proinflammatory phenotype of macrophages and inhibits their anti-inflammatory phenotype. Porous silicon nanocarriers further boost the immunomodulation of mitochondria-targeted BSAs, accompanied by the secretion of proinflammatory mediators. The mechanism involves the delivery of mitochondria-targeted BSA into macrophages to generate mitochondrial ROS and trigger proinflammatory signaling transduction pathways.
Article
Biochemistry & Molecular Biology
Takahiko Matsushita, Hinako Yamochi, Shinzo Omiya, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: Polyacrylamides incorporating serine, aspartic acid, and histidine, the amino acids in the catalytic triad of natural serine protease chymotrypsin, were synthesized and their protein cleavage activity was examined. SDS-PAGE analysis revealed that some of the synthesized ternary copolymers were able to cleave bovine and human serum albumins. Homopolymers incorporating a single type of amino acid also showed protein cleavage abilities. These findings suggest that polymers functionalized with amino acids have potential as proteolytic artificial enzymes.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Alexandra N. Kovacs, Gabor Katona, Adam Juhasz, Gyorgy T. Balogh, Edit Csapo
Summary: The effective applicability of serum albumin-hyaluronic acid (HyA) conjugates as potential drug delivery colloidal particles for encapsulating ibuprofen (IBU) has been demonstrated. These protein/polysaccharide colloidal carriers show improved dissolution, intestinal permeability, and flux features compared to unformulated IBU, making them a more controlled, lower dose loading, and gastric mucosa-sparing therapeutic solution in high-dose IBU therapy.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Inorganic & Nuclear
Martyna Szymanska, Maciej Kubicki, Giovanni N. Roviello, Giuseppe Consiglio, Marta A. Fik-Jaskolka, Violetta Patroniak
Summary: This study reports on a new versatile ligand and its complexes with Cu+ and Ni2+. The metal complexes have potential utility as versatile metal-based protein and DNA binders in the field of new drug discovery.
DALTON TRANSACTIONS
(2022)
Article
Spectroscopy
Farid Nasiri, Gholamreza Dehghan, Masoomeh Shaghaghi, Siavash Datmalchi, Mehrdad Iranshahi
Summary: The study revealed that geranyloxycoumarin (AUR) forms a ground state complex with bovine serum albumin (BSA) primarily through hydrogen bonds and van der Waals interactions. AUR binds to subdomain IIIB of BSA, and the secondary structure of BSA remains unchanged in the presence of AUR.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Ola Grabowska, Sergey A. Samsonov, Lech Chmurzynski, Dariusz Wyrzykowski, Krzysztof Zamojc
Summary: In this study, the binding interactions between highly negative-charged ions (hexacyanoferrates(II/III)) and bovine and human serum albumins (BSA and HSA) were investigated using various techniques. The results showed that hexacyanoferrates(II/III) effectively quenched the intrinsic fluorescence of the albumins through a static mechanism. The strength of the interactions depended on the type of albumin and could be explained by enthalpy-driven processes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Pharmacology & Pharmacy
Sreedhanya SreedharanNair, Krishnaraj Kooramattom Unni, Swaroop Sasidharanpillai, Satheesh Kumar, Charuvila T. Aravindakumar, Usha K. Aravind
Summary: This study investigates the interaction between a pyridine derivative called HDN and serum albumins. The results reveal a moderate binding affinity of HDN with albumins and minimal impact on the protein's secondary structure. This suggests the potential application of HDN as an effective drug.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Yan Tian, Chunhu Li, Fanyu Zeng, Chao Yu, Zhining Xia, Yike Huang
Summary: This study developed a method combining equilibrium dialysis with HPLC and UPLC-MS/MS to study the interactions between multi-components and protein in traditional Chinese medicine (TCM). The experiment showed that the binding affinity of flavones and flavonols with protein was stronger than dihydroflavonoids, and glycoside substitution reduced the binding affinity. Competitive interactions were found among the four flavonoids studied. The method was successfully applied to study the binding of multiple components in Radix Scutellariae (RS) extract with protein.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Dongmei Wu, Michal Gucwa, Mateusz P. Czub, David R. Cooper, Ivan G. Shabalin, Remi Fritzen, Swati Arya, Ulrich Schwarz-Linek, Claudia A. Blindauer, Wladek Minor, Alan J. Stewart
Summary: Serum albumin-Co2+ interactions are clinically important and play a role in cobalt toxicity and the diagnosis of myocardial ischemia. Crystallographic structures of human and equine serum albumin in complex with Co2+ were obtained, revealing prominent metal-binding sites. Mutagenesis and ITC studies characterized the Co2+ binding sites on human serum albumin and showed the presence of weak-affinity Co2+ binding sites. Fatty acids were found to reduce the Co2+ binding affinity. These findings provide a comprehensive understanding of Co2+ binding to serum albumin.
Article
Biochemistry & Molecular Biology
Xu Li, Xiaoyue Sun, Rui Zhao, Di Shao, Shuyun Bi
Summary: The study investigated the interactions of sulfaclozine sodium monohydrate with bovine and human serum albumins using multi-spectroscopy and molecular docking techniques. The main force maintaining the stability of the compound was found to be electrostatic attraction based on thermodynamic parameters. Results from fluorescence measurements and molecular docking showed that SSM interacted with BSA/HSA predominantly at site I.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Engineering, Chemical
Wei Shi, Shun-Nan Zhu, Hong Xu, Kai-Bin Li, Si-Qi Zhang, Tian-Yi Zhang, Chao-Ying Fang, Xiao-Bin Zhang, De-Man Han
Summary: This study explored the potential of dual-ligand hydrophobic charge induction chromatography for simultaneous recovery of bovine serum albumin and bovine immunoglobulin G. Results showed that dual-ligand mode had stable coupling efficiency ratio and broke through the adsorption capacity limit of single-ligand mode. It exhibited good adsorption performance for both components, confirming its effectiveness as a strategy for biological separation.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Alexander V. Fonin, Sergey A. Silonov, Anna S. Fefilova, Olesya V. Stepanenko, Anastasia A. Gavrilova, Alexey V. Petukhov, Anna E. Romanovich, Anna L. Modina, Tatiana S. Zueva, Evgeniy M. Nedelyaev, Nadejda M. Pleskach, Mirya L. Kuranova, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov
Summary: The study revealed that the C-terminal domains of PML isoforms are capable of forming dynamic liquid droplet compartments, regardless of the ordered N-terminal RBCC motifs. This suggests a significant role of non-specific interactions in the liquid-liquid phase separation leading to the formation of PML bodies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anna S. Fefilova, Alexander Fonin, Innokentii E. Vishnyakov, Irina M. Kuznetsova, Konstantin K. Turoverov
Summary: Stress is an inevitable part of life, and organisms have developed mechanisms to combat stress, including the assembly of stress-induced membraneless organelles. These organelles serve to protect the genetic and protein material of cells during stressful conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Olga V. Stepanenko, Irina M. Kuznetsova, Konstantin K. Turoverov, Olesya V. Stepanenko
Summary: Understanding the photophysical properties and stability of near-infrared fluorescent proteins based on bacterial phytochromes is crucial for designing efficient fluorescent probes. It has been found that the covalent binding of the natural ligand biliverdin to these proteins leads to higher quantum yield of fluorescence and increased stability due to rigid fixation of the chromophore in the chromophore-binding pocket.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Irina M. Kuznetsova, Konstantin K. Turoverov
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Anna S. Fefilova, Iuliia A. Antifeeva, Anastasia A. Gavrilova, Konstantin K. Turoverov, Irina M. Kuznetsova, Alexander Fonin
Summary: The discovery of intrinsically disordered proteins (IDPs) has opened up a new era in understanding cellular compartmentalization. Liquid-liquid phase separation (LLPS) plays a major role in the spatial-temporal organization of intracellular space, leading to the formation of membrane-less organelles (MLOs). MLOs are highly dynamic, multicomponent biological condensates that fine-tune the regulation of intracellular processes. IDPs are essential for the assembly and functioning of MLOs. Stress-induced MLOs and the modification of existing MLOs in response to stress factors are described in this review.
Review
Biochemistry & Molecular Biology
Anastasia A. Gavrilova, Anna S. Fefilova, Innokentii E. Vishnyakov, Irina M. Kuznetsova, Konstantin K. Turoverov, Vladimir N. Uversky, Alexander V. Fonin
Summary: At the beginning of the 21st century, it became clear that there were significant changes in the concept of living matter and intracellular organization. Phase transitions of biopolymers, such as intrinsically disordered proteins and RNA, play a crucial role in the spatiotemporal organization of intracellular space. Stress-induced reorganization of intracellular space forms membrane-less organelles, such as nuclear A-bodies and nuclear stress bodies, through liquid-liquid phase separation of intrinsically disordered proteins and non-coding RNA. This review focuses on the functions, structure, and formation mechanism of these membrane-less organelles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Olga Povarova, Iuliia A. Antifeeva, Alexander Fonin, Konstantin K. Turoverov, Irina M. Kuznetsova
Summary: To date, it has been shown that liquid-liquid phase separation (LLPS) plays a role in various cellular processes, providing a new perspective on the spatiotemporal organization of the cell. This new paradigm helps answer long-standing unanswered questions, particularly in the regulation of cytoskeleton assembly/disassembly and actin polymerization. Coacervates of actin-binding proteins formed during LLPS integrate with G-actin to initiate polymerization, while liquid droplet coacervates formed by signaling proteins on the inner side of the cell membrane intensify the activity of actin-binding proteins involved in actin polymerization, such as N-WASP and Arp2/3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yakov I. I. Mokin, Anastasia A. A. Gavrilova, Anna S. S. Fefilova, Irina M. M. Kuznetsova, Konstantin K. K. Turoverov, Vladimir N. N. Uversky, Alexander V. V. Fonin
Summary: In this study, the formation and functioning of membrane-less organelles, A-bodies and nSBs, were investigated. It was found that these organelles have different morphology and function, as well as differences in protein composition and propensity for liquid-liquid phase separation (LLPS). Weak interactions play a crucial role in the formation and functioning of these important organelles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sergey A. Silonov, Eugene Y. Smirnov, Irina M. Kuznetsova, Konstantin K. Turoverov, Alexander V. Fonin
Summary: In this review, the recent progress in understanding the role of individual domains of PML protein, its post-translational modifications, and polyvalent nonspecific interactions in the formation of PML bodies is highlighted. A new hypothetical model of PML body formation is proposed based on the available literature.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)