Article
Cell Biology
Pamina Weber, Doris Baltus, Aline Jatho, Oliver Drews, Laura C. Zelarayan, Thomas Wieland, Susanne Lutz
Summary: The loss of RhoGEF17 disrupts cell-cell and cell-substrate interactions in endothelial cells, preventing cell death and blocking cell proliferation. Non-canonical beta-catenin signaling and Akt activation may play a role in this process.
Article
Neurosciences
Femke M. Feringa, Annemiek A. van Berkel, Anushka Nair, Matthijs Verhage
Summary: Depletion of presynaptic proteins MUNC18-1 or syntaxin-1 triggers an atypical, staged cell death pathway characterized by consecutive neurite retraction, ultimately leading to, but not driven by, apoptosis.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Cell Biology
Hongchen Yan, Jun-Jie Xu, Ilyas Ali, Wei Zhang, Ming Jiang, Guiping Li, Yong Teng, Guangxun Zhu, Yafei Cai
Summary: CDK5RAP3 plays a crucial role in cell growth and interacts with RPL26 to regulate the mTOR pathway. Its deficiency can lead to instability in cell growth and cell cycle regulation.
CELL PROLIFERATION
(2022)
Article
Cell Biology
Cyriel Sebastiaan Olie, Adan Pinto-Fernandez, Andreas Damianou, Iolanda Vendrell, Hailiang Mei, Bianca den Hamer, Erik van der Wal, Jessica C. de Greef, Vered Raz, Benedikt M. Kessler
Summary: The ubiquitin proteasomal system plays a critical role in muscle physiology, but the function of deubiquitinating enzymes (DUBs) in muscle cells remains poorly understood. Through a genetic screen, we discovered that the depletion of USP18, a DUB, affects muscle cell differentiation. USP18 regulates differentiation initiation and maintenance independently of ISG15 and the ISG response. Alterations in gene networks and protein expression profiles were observed in the absence of USP18, leading to disrupted calcium homeostasis and reduced contractile force.
CELL DEATH & DISEASE
(2023)
Review
Medical Laboratory Technology
Tian Zeng, Yang Guan, Yu-kun Li, Qing Wu, Xiao-jun Tang, Xin Zeng, Hui Ling, Juan Zou
Summary: MCM6 plays a significant role in the development of hepatocellular carcinoma by regulating cell cycle, proliferation, and metastasis. Enhanced expression of MCM6 contributes to cancer progression, while inhibition of MCM6 may be an effective treatment method. Further research on the carcinogenic functions of MCM6 could offer new insights into cancer biology and lead to novel approaches for cancer diagnosis and treatment.
CLINICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Toshiki Akui, Kei Fujiwara, Gaku Sato, Masahiro Takinoue, Shin-ichiro M. Nomura, Nobuhide Doi
Summary: Biochemical systems in living cells have an optimal concentration ratio among each constituent element to maintain functionality. However, in systems with complex interactions and feedbacks, the concentration shift of the entire system can greatly change its activity. This study uses a TX-TL system to illustrate the nonlinear relationship between system concentration and activity, showing how concentration shifts can act as a switch for system activity and influence its function.
Article
Cell Biology
Vijay Swahari, Ayumi Nakamura, Emilie Hollville, Hume Stroud, Jeremy M. Simon, Travis S. Ptacek, Matthew Beck, Cornelius Flowers, Jiami Guo, Charlotte Plestant, Jie Liang, C. Lisa Kurtz, Matt Kanke, Scott M. Hammond, You-Wen He, E. S. Anton, Praveen Sethupathy, Sheryl S. Moy, Michael E. Greenberg, Mohanish Deshmukh
Summary: The miR-29 family is significantly induced during the late stages of brain maturation, and plays a crucial role in restricting CH methylation in the brain through targeting of Dnmt3a. Deletion of miR-29 results in neurological deficits, premature lethality, and increased DNMT3A expression, highlighting the importance of CH methylation regulation for normal brain maturation.
Review
Biochemistry & Molecular Biology
Kewal Kumar Mahapatra, Soumya Ranjan Mishra, Bishnu Prasad Behera, Shankargouda Patil, David A. Gewirtz, Sujit Kumar Bhutia
Summary: Lysosomes are crucial organelles responsible for degrading cellular cargo and maintaining cellular homeostasis, playing a critical role in the connection between autophagy and endocytosis. Damage to the lysosomal membrane can lead to selective lysosomal degradation pathway and trigger lysophagy in severe cases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Genetics & Heredity
Katherine J. J. Wozniak, Peter E. E. Burby, Jayakrishnan Nandakumar, Lyle A. A. Simmons
Summary: The study investigates the role of kinase CcrZ in regulating DNA replication initiation in Bacillus subtilis. The researchers identified the ccrZ gene and found that its deletion results in sensitivity to DNA damage. They demonstrate that increased expression of ccrZ leads to over-initiation of DNA replication. The crystal structure analysis reveals a major restructuring of the substrate-binding site, indicating that CcrZ has activity on novel substrates rather than choline. This study provides important insights into the function of bacterial kinases in regulating DNA replication initiation.
Article
Multidisciplinary Sciences
A. Kaidow, N. Ishii, S. Suzuki, T. Shiina, K. Endoh, Y. Murakami, H. Kasahara
Summary: It has been discovered that cells with chromosome damage and defective recombinase activity can survive under appropriate oxidative stress levels. By reducing intracellular reactive oxygen species (ROS) levels, cells become sensitive to ROS rather than chronic chromosome damage. Additionally, expression of the Hsp33 protein enhances cell survival and maintains an appropriate oxidative stress level in cells with oxidative damage, rescuing cell growth.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Physical
Teng Liu, Pei Pei, Wenhao Shen, Lin Hu, Kai Yang
Summary: Traditional radiotherapy (RT) is limited by radioresistance, while immunotherapy has low response rate and high cost. The combination of radiotherapy and immunotherapy in radioimmunotherapy shows promise in eliminating cancer cells with specificity, efficiency, and safety. RT-induced immunogenic cell death (ICD) plays a crucial role in stimulating systemic immune response against cancer, and this review discusses the origin, concept, signaling pathways, and enhancing strategies of RT-induced ICD for radioimmunotherapy. It also attempts to forecast future directions for clinical applications based on published research and underlying mechanisms.
Article
Biochemistry & Molecular Biology
Rene Huber, Mareike Diekmann, Leonie Hoffmeister, Friederike Kuhl, Bastian Welz, Korbinian Brand
Summary: The research investigated the impact of MARCKS on monocytic cell functions, revealing that MARCKS is an essential molecule for ROS production in monocytic cells and is involved in a signaling cascade for ROS formation.
Article
Biochemistry & Molecular Biology
Pritha Choudhury, Atish Barua, Anup Roy, Rudradip Pattanayak, Maitree Bhattacharyya, Prosenjit Saha
Summary: This study demonstrates the potential of eugenol as an effective chemopreventive agent in inhibiting lung cancer progression, especially targeting drug-resistant and virulent cancer cell subpopulations. By affecting regulator molecules like beta-catenin, eugenol induces apoptosis and suppresses cell proliferation in lung tissue, exhibiting exceptional chemopreventive qualities. The molecular mechanism involves the restriction of beta-catenin nuclear transportation and suppression of CSC markers, reducing the aggressive nature of lung carcinoma.
Article
Multidisciplinary Sciences
Joo-Won Kim, Dong Wha Min, Dasom Kim, Joohee Kim, Min Jung Kim, Hyangsoon Lim, Ji-Yun Lee
Summary: Ferroptosis can be induced through various mechanisms such as inhibiting antioxidant enzymes and lipid peroxidation. This study focused on investigating the effects of ferroptosis inducers on non-small cell lung cancer (NSCLC) cells and found that RSL3 was more effective than erastin in inducing cell death. The sensitivity of NSCLC cells to RSL3-induced death was dependent on GPX4 expression levels and was reversed by a ferroptosis inhibitor. RSL3 induced ferroptosis by promoting lipid peroxidation and disrupting the GSH conversion.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ferhat Buke, Jacopo Grilli, Marco Cosentino Lagomarsino, Gregory Bokinsky, Sander J. Tans
Summary: Cell size is regulated by growth and division. Bacteria achieve size homeostasis by dividing when growth adds a constant size since birth, a principle known as the adder principle. In this study, the researchers found that the concentration of guanosine tetraphosphate (ppGpp) in cells not only controls the growth rate, but also regulates cell division and steady-state cell size. The added size and steady-state birth size correlate consistently with the ppGpp concentration, rather than with the growth rate.
Article
Neurosciences
Sung Young Park, Sang-Ho Song, Brandon Palmateer, Akash Pal, Eric D. Petersen, Gabrielle P. Shall, Ryan M. Welchko, Keiji Ibata, Atsushi Miyawaki, George J. Augustine, Ute Hochgeschwender
JOURNAL OF NEUROSCIENCE RESEARCH
(2020)
Article
Multidisciplinary Sciences
Mikiko Fukuda, Asako Sakaue-Sawano, Chikako Shimura, Makoto Tachibana, Atsushi Miyawaki, Yoichi Shinkai
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Margarete Diaz-Cuadros, Daniel E. Wagner, Christoph Budjan, Alexis Hubaud, Oscar A. Tarazona, Sophia Donelly, Arthur Michaut, Ziad Al Tanoury, Kumiko Yoshioka-Kobayashi, Yusuke Niino, Ryoichiro Kageyama, Atsushi Miyawaki, Jonathan Touboul, Olivier Pourquie
Article
Multidisciplinary Sciences
Kumiko Yoshioka-Kobayashi, Marina Matsumiya, Yusuke Niino, Akihiro Isomura, Hiroshi Kori, Atsushi Miyawaki, Ryoichiro Kageyama
Article
Multidisciplinary Sciences
Shunji Nakano, Muneki Ikeda, Yuki Tsukada, Xianfeng Fei, Takamasa Suzuki, Yusuke Niino, Rhea Ahluwalia, Ayana Sano, Rumi Kondo, Kunio Ihara, Atsushi Miyawaki, Koichi Hashimoto, Tetsuya Higashiyama, Ikue Mori
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Hiroyuki Katayama, Hiroshi Hama, Koji Nagasawa, Hiroshi Kurokawa, Mayu Sugiyama, Ryoko Ando, Masaaki Funata, Nobuyo Yoshida, Misaki Homma, Takanori Nishimura, Megumu Takahashi, Yoko Ishida, Hiroyuki Hioki, Yoshiyuki Tsujihata, Atsushi Miyawaki
Article
Chemistry, Medicinal
Nobuo Kitada, Ryohei Saito, Rika Obata, Satoshi Iwano, Kazuma Karube, Atsushi Miyawaki, Takashi Hirano, Shojiro A. Maki
Review
Biotechnology & Applied Microbiology
Satoshi Furukawa, Aiko Nagamatsu, Mitsuru Nenoi, Akira Fujimori, Shizuko Kakinuma, Takanori Katsube, Bing Wang, Chizuru Tsuruoka, Toshiyuki Shirai, Asako J. Nakamura, Asako Sakaue-Sawano, Atsushi Miyawaki, Hiroshi Harada, Minoru Kobayashi, Junya Kobayashi, Takekazu Kunieda, Tomoo Funayama, Michiyo Suzuki, Tatsuo Miyamoto, Jun Hidema, Yukari Yoshida, Akihisa Takahashi
BIOMED RESEARCH INTERNATIONAL
(2020)
Article
Multidisciplinary Sciences
Shuo Chen, Linmeng He, Arthur J. Y. Huang, Roman Boehringer, Vincent Robert, Marie E. Wintzer, Denis Polygalov, Adam Z. Weitemier, Yanqiu Tao, Mingxiao Gu, Steven J. Middleton, Kana Namiki, Hiroshi Hama, Ludivine Therreau, Vivien Chevaleyre, Hiroyuki Hioki, Atsushi Miyawaki, Rebecca A. Piskorowski, Thomas J. McHugh
Article
Surgery
Yoshiharu Kono, Takeaki Ishizawa, Norihiro Kokudo, Yugo Kuriki, Ryu J. Iwatate, Mako Kamiya, Yasuteru Urano, Akiko Kumagai, Hiroshi Kurokawa, Atsushi Miyawaki, Kiyoshi Hasegawa
WORLD JOURNAL OF SURGERY
(2020)
Article
Materials Science, Multidisciplinary
Felix Sima, Hiroyuki Kawano, Masahiko Hirano, Atsushi Miyawaki, Kotaro Obata, Daniela Serien, Koji Sugioka
ADVANCED MATERIALS TECHNOLOGIES
(2020)
Review
Multidisciplinary Sciences
Ken Takahashi, Hideyuki Takahashi, Takuya Furuichi, Masatsugu Toyota, Makoto Furutani-Seiki, Takeshi Kobayashi, Haruko Watanabe-Takano, Masahiro Shinohara, Takuro Numaga-Tomita, Asako Sakaue-Sawano, Atsushi Miyawaki, Keiji Naruse
Summary: Gravity determines the shape and functions of body tissues in plants and animals, and cellular response to gravity is an active process of mechanotransduction. Despite sharing common mechanisms, each species has its own way of sensing and responding to gravity. Understanding cellular responses to gravity is important not only for life on Earth, but also for laying the foundation for living in space in the age of Mars exploration.
Proceedings Paper
Nanoscience & Nanotechnology
Daniela Serien, Hiroyuki Kawano, Atsushi Miyawaki, Koji Sugioka
LASER-BASED MICRO- AND NANOPROCESSING XIV
(2020)
Meeting Abstract
Biophysics
Yoh Shitashima, Atsushi Miyawaki
BIOPHYSICAL JOURNAL
(2020)